Restriction map for
pFN21ASDA1722
(Circular) (Six-base) MAPSORT of: pFN21ASDA1722.seq Check: 7615 from: 1 to: 9226
pFN21ASDA1722 9226 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7036 278
Size: 53 83 186 936 5500 2468
Fragments arranged by size:
5500 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2949 3438 6569 1937
Size: 1012 489 3131 4594
Fragments arranged by size:
4594 3131 1012 489
Acc65I G'GTAC_C
Cuts at: 6547 6547
Size: 9226
AclI AA'CG_TT
Cuts at: 1563 7353 7726 1563
Size: 5790 373 3063
Fragments arranged by size:
5790 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4190 4418 5530 7289 8673 8941 1632
Size: 2558 228 1112 1759 1384 268 1917
Fragments arranged by size:
2558 1917 1759 1384 1112 268 228
AflII C'TTAA_G
Cuts at: 820 1017 2674 4720 6092 820
Size: 197 1657 2046 1372 3954
Fragments arranged by size:
3954 2046 1657 1372 197
AflIII A'CryG_T
Cuts at: 1203 8125 9102 1203
Size: 6922 977 1327
Fragments arranged by size:
6922 1327 977
AgeI A'CCGG_T
Cuts at: 8057 8057
Size: 9226
AhdI GACnn_n'nnGTC
Cuts at: 7955 7955
Size: 9226
AleI CACnn'nnGTG
Cuts at: 1166 2750 1166
Size: 1584 7642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2375 2768 5504 1184
Size: 1191 393 2736 4906
Fragments arranged by size:
4906 2736 1191 393
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6291 6323 6291
Size: 32 9194
AlwNI CAG_nnn'CTG
Cuts at: 3424 6484 6969 8541 3424
Size: 3060 485 1572 4109
Fragments arranged by size:
4109 3060 1572 485
ApaI G_GGCC'C
Cuts at: 5577 5577
Size: 9226
ApaLI G'TGCA_C
Cuts at: 7283 8439 7283
Size: 1156 8070
ApoI r'AATT_y
Cuts at: 1493 2052 2315 3146 3494 3784 6110 6535 6761
Size: 559 263 831 348 290 2326 425 226
Cuts at: 6761 6913 9022 1493
Size: 152 2109 1697
Fragments arranged by size:
2326 2109 1697 831 559 425 348 290
263 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4121 4260 7780 160
Size: 872 3089 139 3520 1606
Fragments arranged by size:
3520 3089 1606 872 139
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9226
AvaI C'yCGr_G
Cuts at: 1870 1943 2155 3849 4935 6551 1870
Size: 73 212 1694 1086 1616 4545
Fragments arranged by size:
4545 1694 1616 1086 212 73
BamHI G'GATC_C
Cuts at: 6556 6923 6556
Size: 367 8859
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5767 5978 6359 6547 8002
Size: 325 217 627 3980 211 381 188 1455
Cuts at: 8002 618
Size: 1842
Fragments arranged by size:
3980 1842 1455 627 381 325 217 211
188
BanII G_rGCy'C
Cuts at: 1391 3877 5577 6354 6545 1391
Size: 2486 1700 777 191 4072
Fragments arranged by size:
4072 2486 1700 777 191
BbeI G_GCGC'C
Cuts at: 6363 6363
Size: 9226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5022 5229 5661 6127 928
Size: 971 3123 207 432 466 4027
Fragments arranged by size:
4027 3123 971 466 432 207
BbvCI CC'TCA_GC
Cuts at: 2195 2707 3290 2195
Size: 512 583 8131
Fragments arranged by size:
8131 583 512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2128 2162 4874 4908 7417 7451 1903
Size: 34 191 34 2712 34 2509 34 3678
Fragments arranged by size:
3678 2712 2509 191 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3238 7118 8328 3238
Size: 3880 1210 4136
Fragments arranged by size:
4136 3880 1210
BclI T'GATC_A
Cuts at: 1548 2326 5920 1548
Size: 778 3594 4854
Fragments arranged by size:
4854 3594 778
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4622 7837 136
Size: 107 122 71 631 605 2950 3215 1525
Fragments arranged by size:
3215 2950 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4818 9221 4818
Size: 4403 4823
BlpI GC'TnA_GC
Cuts at: 3552 6643 3552
Size: 3091 6135
Bme1580I G_kGCm'C
Cuts at: 1790 5346 5577 5772 6320 7287 8443 1790
Size: 3556 231 195 548 967 1156 2573
Fragments arranged by size:
3556 2573 1156 967 548 231 195
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9226
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2257 5392 6460
Size: 416 53 83 399 24 840 3135 1068
Cuts at: 6460 6957 7915 442
Size: 497 958 1753
Fragments arranged by size:
3135 1753 1068 958 840 497 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4200 4341 1056
Size: 3144 141 5941
Fragments arranged by size:
5941 3144 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6226 6226
Size: 9226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2465 2675 2913 3362 3446 3560 5090
Size: 1052 174 210 238 449 84 114 1530
Cuts at: 5090 5775 7886 1239
Size: 685 2111 2579
Fragments arranged by size:
2579 2111 1530 1052 685 449 238 210
174 114 84
Bpu10I CC'TnA_GC
Cuts at: 2195 2707 3104 3290 2195
Size: 512 397 186 8131
Fragments arranged by size:
8131 512 397 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2540 2795 2990 4743 4835 7351 8216 8514 2540
Size: 255 195 1753 92 2516 865 298 3252
Fragments arranged by size:
3252 2516 1753 865 298 255 195 92
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2696 3170 6232 7889 882
Size: 617 1197 474 3062 1657 2219
Fragments arranged by size:
3062 2219 1657 1197 617 474
BsaAI yAC'GTr
Cuts at: 493 1204 4634 6523 7017 493
Size: 711 3430 1889 494 2702
Fragments arranged by size:
3430 2702 1889 711 494
BsaBI GATnn'nnATC
Cuts at: 4362 9220 4362
Size: 4858 4368
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3980 6261 6360 7033
Size: 53 83 186 936 2447 2281 99 673
Cuts at: 7033 7415 8050 275
Size: 382 635 1451
Fragments arranged by size:
2447 2281 1451 936 673 635 382 186
99 83 53
BsaWI w'CCGG_w
Cuts at: 3771 5535 5580 7659 8057 8331 8478 8889 3771
Size: 1764 45 2079 398 274 147 411 4108
Fragments arranged by size:
4108 2079 1764 411 398 274 147 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 2205 2235 3612
Size: 30 165 30 768 30 4 30 1377
Cuts at: 3612 3642 4532 4562 5321 5351 6065 6095 1178
Size: 30 890 30 759 30 714 30 4309
Fragments arranged by size:
4309 1377 890 768 759 714 165 30
30 30 30 30 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4155 4763 5606 5680 1186
Size: 2969 608 843 74 4732
Fragments arranged by size:
4732 2969 843 608 74
BseYI C'CCAG_C
Cuts at: 2437 3557 3701 3709 4078 4803 5515 5569 6368
Size: 1120 144 8 369 725 712 54 799
Cuts at: 6368 6495 8429 8925 2437
Size: 127 1934 496 2738
Fragments arranged by size:
2738 1934 1120 799 725 712 496 369
144 127 54 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4428 4490 4673 1163
Size: 573 2692 62 183 5716
Fragments arranged by size:
5716 2692 573 183 62
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3764 6070 6676 7437 8465 1540
Size: 275 186 1763 2306 606 761 1028 2301
Fragments arranged by size:
2306 2301 1763 1028 761 606 275 186
BsiHKAI G_wGCw'C
Cuts at: 3738 3877 5858 7287 7372 8443 3738
Size: 139 1981 1429 85 1071 4521
Fragments arranged by size:
4521 1981 1429 1071 139 85
BsmI GAATG_Cn'
Cuts at: 6737 6830 6737
Size: 93 9133
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3738 3877 5346 5577 5772 5858 6320
Size: 399 1948 139 1469 231 195 86 462
Cuts at: 6320 6354 6545 7287 7372 8443 1391
Size: 34 191 742 85 1071 2174
Fragments arranged by size:
2174 1948 1469 1071 742 462 399 231
195 191 139 86 85 34
BspEI T'CCGG_A
Cuts at: 5580 8889 5580
Size: 3309 5917
BspHI T'CATG_A
Cuts at: 6192 7115 8845 6192
Size: 923 1730 6573
Fragments arranged by size:
6573 1730 923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4974 6581 844
Size: 4130 1607 3489
Fragments arranged by size:
4130 3489 1607
BsrBI CCG'CTC
Cuts at: 1394 1823 2265 2644 4951 7113 1394
Size: 429 442 379 2307 2162 3507
Fragments arranged by size:
3507 2307 2162 442 429 379
BsrDI GCAATG_nn'
Cuts at: 65 1248 7721 7895 65
Size: 1183 6473 174 1396
Fragments arranged by size:
6473 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4083 4569 7870 8057 1700
Size: 110 2273 486 3301 187 2869
Fragments arranged by size:
3301 2869 2273 486 187 110
BsrGI T'GTAC_A
Cuts at: 96 3881 5493 96
Size: 3785 1612 3829
Fragments arranged by size:
3829 3785 1612
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9226
BssSI C'ACGA_G
Cuts at: 7286 8298 7286
Size: 1012 8214
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6082 6484 794
Size: 5288 402 3536
Fragments arranged by size:
5288 3536 402
BstBI TT'CG_AA
Cuts at: 2007 8030 2007
Size: 6023 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9226
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2611 5216 2029
Size: 582 2605 6039
Fragments arranged by size:
6039 2605 582
BstYI r'GATC_y
Cuts at: 1972 2714 4396 4818 5185 6556 6923 7308 7325
Size: 742 1682 422 367 1371 367 385 17
Cuts at: 7325 8777 8863 8875 9221 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1682 1452 1371 742 422 385 367
367 346 86 17 12
BstZ17I GTA'TAC
Cuts at: 3439 3439
Size: 9226
Bsu36I CC'TnA_GG
Cuts at: 3932 5256 5687 3932
Size: 1324 431 7471
Fragments arranged by size:
7471 1324 431
BtgI C'CryG_G
Cuts at: 513 1065 3182 3997 5292 5660 5970 6384 513
Size: 552 2117 815 1295 368 310 414 3355
Fragments arranged by size:
3355 2117 1295 815 552 414 368 310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8487
Fragments arranged by size:
8487 683 56
BtsI GCAGTG_nn'
Cuts at: 5024 6320 6482 6742 7536 7556 5024
Size: 1296 162 260 794 20 6694
Fragments arranged by size:
6694 1296 794 260 162 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9191
DraI TTT'AAA
Cuts at: 3331 3613 4837 6530 3331
Size: 282 1224 1693 6027
Fragments arranged by size:
6027 1693 1224 282
DraIII CAC_nnn'GTG
Cuts at: 2302 2302
Size: 9226
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5630 6017 8233 809
Size: 507 4314 387 2216 1802
Fragments arranged by size:
4314 2216 1802 507 387
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3761 3858 5723
Size: 54 1425 50 275 18 1931 97 1865
Cuts at: 5723 6673 7562 9191 8
Size: 950 889 1629 43
Fragments arranged by size:
1931 1865 1629 1425 950 889 275 97
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3761 6673 1537
Size: 275 1949 2912 4090
Fragments arranged by size:
4090 2912 1949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3168 3375 4055 4911 5981 7156 1353
Size: 1815 207 680 856 1070 1175 3423
Fragments arranged by size:
3423 1815 1175 1070 856 680 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5127 7813 8187 8333 387
Size: 1064 138 3538 2686 374 146 1280
Fragments arranged by size:
3538 2686 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3875 3875
Size: 9226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2465 2675 2913 3362 3446 3560
Size: 393 659 174 210 238 449 84 114
Cuts at: 3560 4190 4418 5090 5530 5775 7289 7886 8673
Size: 630 228 672 440 245 1514 597 787
Cuts at: 8673 8941 1239
Size: 268 1524
Fragments arranged by size:
1524 1514 787 672 659 630 597 449
440 393 268 245 238 228 210 174
114 84
EcoNI CCTnn'n_nnAGG
Cuts at: 6095 6161 6095
Size: 66 9160
EcoO109I rG'GnC_Cy
Cuts at: 5573 5573
Size: 9226
EcoRI G'AATT_C
Cuts at: 2052 3146 6535 6913 2052
Size: 1094 3389 378 4365
Fragments arranged by size:
4365 3389 1094 378
EcoRV GAT'ATC
Cuts at: 4987 4987
Size: 9226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3860 4330 3860
Size: 470 8756
FspI TGC'GCA
Cuts at: 7732 7732
Size: 9226
HaeII r_GCGC'y
Cuts at: 1678 4698 6363 8373 1678
Size: 3020 1665 2010 2531
Fragments arranged by size:
3020 2531 2010 1665
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 3733 3765 3859
Size: 32 135 32 599 32 1530 32 94
Cuts at: 3859 3891 4935 4967 5072 5104 5429 5461 5588
Size: 32 1044 32 105 32 325 32 127
Cuts at: 5588 5620 5887 5919 7867 7899 7941 7973 1373
Size: 32 267 32 1948 32 42 32 2626
Fragments arranged by size:
2626 1948 1530 1044 599 325 267 135
127 105 94 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4132 4187 6570 6816 669
Size: 988 281 2194 55 2383 246 3079
Fragments arranged by size:
3079 2383 2194 988 281 246 55
HindIII A'AGCT_T
Cuts at: 748 2393 748
Size: 1645 7581
HpaI GTT'AAC
Cuts at: 4132 6816 4132
Size: 2684 6542
KasI G'GCGC_C
Cuts at: 6359 6359
Size: 9226
KpnI G_GTAC'C
Cuts at: 6551 6551
Size: 9226
MfeI C'AATT_G
Cuts at: 6825 6825
Size: 9226
MluI A'CGCG_T
Cuts at: 9102 9102
Size: 9226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5427 8340 8524 1305
Size: 4122 2913 184 2007
Fragments arranged by size:
4122 2913 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3860 10
Size: 54 1425 343 2028 5376
Fragments arranged by size:
5376 2028 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2072 2609 2750 5214 6233 7184
Size: 648 861 45 537 141 2464 1019 951
Cuts at: 7184 7543 7702 518
Size: 359 159 2042
Fragments arranged by size:
2464 2042 1019 951 861 648 537 359
159 141 45
MspA1I CmG'CkG
Cuts at: 1606 2405 2437 3705 5412 5662 5880 6642 7319
Size: 799 32 1268 1707 250 218 762 677
Cuts at: 7319 8467 8712 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1707 1268 1148 799 762 677 250
245 218 32
NaeI GCC'GGC
Cuts at: 4085 4085
Size: 9226
NarI GG'CG_CC
Cuts at: 6360 6360
Size: 9226
NcoI C'CATG_G
Cuts at: 513 1065 3182 5292 513
Size: 552 2117 2110 4447
Fragments arranged by size:
4447 2117 2110 552
NdeI CA'TA_TG
Cuts at: 387 4878 387
Size: 4491 4735
NgoMIV G'CCGG_C
Cuts at: 4083 4083
Size: 9226
NheI G'CTAG_C
Cuts at: 1052 4196 4337 1052
Size: 3144 141 5941
Fragments arranged by size:
5941 3144 141
NotI GC'GGCC_GC
Cuts at: 6673 6673
Size: 9226
NspI r_CATG'y
Cuts at: 1132 1229 3699 6034 6089 6584 8129 1132
Size: 97 2470 2335 55 495 1545 2229
Fragments arranged by size:
2470 2335 2229 1545 495 97 55
PasI CC'CwG_GG
Cuts at: 1348 5608 1348
Size: 4260 4966
PciI A'CATG_T
Cuts at: 8125 8125
Size: 9226
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2698 3424 5935 1108
Size: 1590 726 2511 4399
Fragments arranged by size:
4399 2511 1590 726
PfoI T'CCnGG_A
Cuts at: 3364 4296 4707 3364
Size: 932 411 7883
Fragments arranged by size:
7883 932 411
PmeI GTTT'AAAC
Cuts at: 6530 6530
Size: 9226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7295 7327 8808 8840 7295
Size: 32 1481 32 7681
Fragments arranged by size:
7681 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9226
PsiI TTA'TAA
Cuts at: 5616 6796 5616
Size: 1180 8046
PspOMI G'GGCC_C
Cuts at: 5573 5573
Size: 9226
PstI C_TGCA'G
Cuts at: 830 2364 3259 4971 5910 6578 830
Size: 1534 895 1712 939 668 3478
Fragments arranged by size:
3478 1712 1534 939 895 668
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9226
PvuII CAG'CTG
Cuts at: 2405 2437 3705 2405
Size: 32 1268 7926
Fragments arranged by size:
7926 1268 32
SacI G_AGCT'C
Cuts at: 3877 3877
Size: 9226
SacII CC_GC'GG
Cuts at: 5663 5663
Size: 9226
SalI G'TCGA_C
Cuts at: 1936 6568 1936
Size: 4632 4594
SapI GCTCTTCn'nnn_
Cuts at: 5981 5981
Size: 9226
SbfI CC_TGCA'GG
Cuts at: 4971 6578 4971
Size: 1607 7619
ScaI AGT'ACT
Cuts at: 7474 9186 7474
Size: 1712 7514
SexAI A'CCwGG_T
Cuts at: 4320 4320
Size: 9226
SfcI C'TryA_G
Cuts at: 826 1045 2360 2835 2987 3255 4698 4792 4903
Size: 219 1315 475 152 268 1443 94 111
Cuts at: 4903 4967 5906 6574 7709 8390 8581 826
Size: 64 939 668 1135 681 191 1471
Fragments arranged by size:
1471 1443 1315 1135 939 681 668 475
268 219 191 152 111 94 64
SfoI GGC'GCC
Cuts at: 6361 6361
Size: 9226
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9226
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9226
SmaI CCC'GGG
Cuts at: 1872 4937 6553 1872
Size: 3065 1616 4545
Fragments arranged by size:
4545 3065 1616
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 2674 2810 3005 4720 4758
Size: 197 926 612 119 136 195 1715 38
Cuts at: 4758 4814 6092 7330 8231 8493 820
Size: 56 1278 1238 901 262 1553
Fragments arranged by size:
1715 1553 1278 1238 926 901 612 262
197 195 136 119 56 38
SnaBI TAC'GTA
Cuts at: 493 6523 7017 493
Size: 6030 494 2702
Fragments arranged by size:
6030 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9226
SphI G_CATG'C
Cuts at: 1132 6089 6584 1132
Size: 4957 495 3774
Fragments arranged by size:
4957 3774 495
SspI AAT'ATT
Cuts at: 5 52 3518 5674 7150 5
Size: 47 3466 2156 1476 2081
Fragments arranged by size:
3466 2156 2081 1476 47
StuI AGG'CCT
Cuts at: 1517 2924 4616 4901 1517
Size: 1407 1692 285 5842
Fragments arranged by size:
5842 1692 1407 285
StyI C'CwwG_G
Cuts at: 513 1065 3182 3374 5203 5292 5312 5359 6665
Size: 552 2117 192 1829 89 20 47 1306
Cuts at: 6665 513
Size: 3074
Fragments arranged by size:
3074 2117 1829 1306 552 192 89 47
20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2108 3563 3696 4842 5372 6472 7253
Size: 1255 528 1455 133 1146 530 1100 781
Cuts at: 7253 7270 7423 7608 8053 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1455 1255 1146 1100 781 530 528
445 185 153 133 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 3881 5493
Size: 274 80 33 51 1443 441 1463 1612
Cuts at: 5493 5832 7472 9184 96
Size: 339 1640 1712 138
Fragments arranged by size:
1712 1640 1612 1463 1443 441 339 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 9186 9218 2205
Size: 32 6949 32 2213
Fragments arranged by size:
6949 2213 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2945 1316
Size: 1629 7597
XbaI T'CTAG_A
Cuts at: 3085 5449 6562 3085
Size: 2364 1113 5749
Fragments arranged by size:
5749 2364 1113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 2725 5299 5548 2117
Size: 608 2574 249 5795
Fragments arranged by size:
5795 2574 608 249
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9226
XmaI C'CCGG_G
Cuts at: 1870 4935 6551 1870
Size: 3065 1616 4545
Fragments arranged by size:
4545 3065 1616
XmnI GAAnn'nnTTC
Cuts at: 3225 3495 3971 7355 3225
Size: 270 476 3384 5096
Fragments arranged by size:
5096 3384 476 270
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7034 276
Size: 53 83 186 936 5500 2468
Fragments arranged by size:
5500 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SanDI SfiI SrfI SwaI