Restriction map for
pFN21ASDA1720
(Circular) (Six-base) MAPSORT of: pFN21ASDA1720.seq Check: 5529 from: 1 to: 5908
pFN21ASDA1720 5908 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3718 278
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3251 1937
Size: 1314 4594
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 4035 4408 1563
Size: 2472 373 3063
Fragments arranged by size:
3063 2472 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2103 3033 3971 5355 5623 1632
Size: 471 930 938 1384 268 1917
Fragments arranged by size:
1917 1384 938 930 471 268
AfeI AGC'GCT
Cuts at: 2424 2424
Size: 5908
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5711
AflIII A'CryG_T
Cuts at: 1203 4807 5784 1203
Size: 3604 977 1327
Fragments arranged by size:
3604 1327 977
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2475 1184
Size: 1291 4617
AlwNI CAG_nnn'CTG
Cuts at: 2728 3651 5223 2728
Size: 923 1572 3413
Fragments arranged by size:
3413 1572 923
ApaI G_GGCC'C
Cuts at: 2879 2994 2879
Size: 115 5793
ApaLI G'TGCA_C
Cuts at: 3965 5121 3965
Size: 1156 4752
ApoI r'AATT_y
Cuts at: 1493 3217 3443 3595 5704 1493
Size: 1724 226 152 2109 1697
Fragments arranged by size:
2109 1724 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4462 160
Size: 872 3430 1606
Fragments arranged by size:
3430 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 2463 2640 2915 3233 1870
Size: 73 520 177 275 318 4545
Fragments arranged by size:
4545 520 318 275 177 73
AvrII C'CTAG_G
Cuts at: 2105 2105
Size: 5908
BamHI G'GATC_C
Cuts at: 2191 2292 3238 3605 2191
Size: 101 946 367 4494
Fragments arranged by size:
4494 946 367 101
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2844 3144 3180 3229 4684
Size: 325 217 627 1057 300 36 49 1455
Cuts at: 4684 618
Size: 1842
Fragments arranged by size:
1842 1455 1057 627 325 300 217 49
36
BanII G_rGCy'C
Cuts at: 1391 2034 2114 2706 2879 2994 3169 3227 1391
Size: 643 80 592 173 115 175 58 4072
Fragments arranged by size:
4072 643 592 175 173 115 80 58
BbeI G_GCGC'C
Cuts at: 2848 3148 2848
Size: 300 5608
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2666 928
Size: 971 767 4170
Fragments arranged by size:
4170 971 767
BbvCI CC'TCA_GC
Cuts at: 2634 2634
Size: 5908
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4099 4133 1903
Size: 34 2162 34 3678
Fragments arranged by size:
3678 2162 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4519 136
Size: 107 122 71 631 605 2847 1525
Fragments arranged by size:
2847 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5903 5903
Size: 5908
BlpI GC'TnA_GC
Cuts at: 2028 2400 2484 3029 3325 2028
Size: 372 84 545 296 4611
Fragments arranged by size:
4611 545 372 296 84
Bme1580I G_kGCm'C
Cuts at: 1790 2879 2994 3969 5125 1790
Size: 1089 115 975 1156 2573
Fragments arranged by size:
2573 1156 1089 975 115
BmgBI CAC'GTC
Cuts at: 1318 3103 1318
Size: 1785 4123
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2108 2707 3639
Size: 416 53 83 399 24 691 599 932
Cuts at: 3639 4597 442
Size: 958 1753
Fragments arranged by size:
1753 958 932 691 599 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2151 2750 2780 2834 2990 4568 1239
Size: 912 599 30 54 156 1578 2579
Fragments arranged by size:
2579 1578 912 599 156 54 30
Bpu10I CC'TnA_GC
Cuts at: 2391 2634 2775 2391
Size: 243 141 5524
Fragments arranged by size:
5524 243 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2690 4033 4898 5196 2690
Size: 1343 865 298 3402
Fragments arranged by size:
3402 1343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 4571 882
Size: 617 552 2520 2219
Fragments arranged by size:
2520 2219 617 552
BsaAI yAC'GTr
Cuts at: 493 1204 2526 3205 3699 493
Size: 711 1322 679 494 2702
Fragments arranged by size:
2702 1322 711 679 494
BsaBI GATnn'nnATC
Cuts at: 5902 5902
Size: 5908
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2493 2845 3145 3715
Size: 53 83 186 936 960 352 300 570
Cuts at: 3715 4097 4732 275
Size: 382 635 1451
Fragments arranged by size:
1451 960 936 635 570 382 352 300
186 83 53
BsaWI w'CCGG_w
Cuts at: 2667 4341 4739 5013 5160 5571 2667
Size: 1674 398 274 147 411 3004
Fragments arranged by size:
3004 1674 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5683
Fragments arranged by size:
5683 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2148 2192 2222 2252 2747 2878 2987 1186
Size: 962 44 30 30 495 131 109 4107
Fragments arranged by size:
4107 962 495 131 109 44 30 30
BseYI C'CCAG_C
Cuts at: 3124 5111 5607 3124
Size: 1987 496 3425
Fragments arranged by size:
3425 1987 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5335
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3358 4119 5147 1540
Size: 275 186 1357 761 1028 2301
Fragments arranged by size:
2301 1357 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2220 2604 2706 2739 3081 3969 4054 5125
Size: 186 384 102 33 342 888 85 1071
Cuts at: 5125 2034
Size: 2817
Fragments arranged by size:
2817 1071 888 384 342 186 102 85
33
BsmI GAATG_Cn'
Cuts at: 3419 3512 3419
Size: 93 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2114 2220 2604 2706 2739 2879
Size: 399 244 80 106 384 102 33 140
Cuts at: 2879 2994 3081 3169 3227 3969 4054 5125 1391
Size: 115 87 88 58 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 384 244 140 115
106 102 88 87 85 80 58 33
BspEI T'CCGG_A
Cuts at: 2667 5571 2667
Size: 2904 3004
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2561 2758 3063 3263 844
Size: 1717 197 305 200 3489
Fragments arranged by size:
3489 1717 305 200 197
BsrBI CCG'CTC
Cuts at: 1394 1823 2437 2581 2596 3795 1394
Size: 429 614 144 15 1199 3507
Fragments arranged by size:
3507 1199 614 429 144 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 4403 4577 65
Size: 1183 3155 174 1396
Fragments arranged by size:
3155 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4552 4739 1700
Size: 110 2742 187 2869
Fragments arranged by size:
2869 2742 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5908
BssSI C'ACGA_G
Cuts at: 3968 4980 3968
Size: 1012 4896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2536 794
Size: 1742 4166
BstBI TT'CG_AA
Cuts at: 2007 4712 2007
Size: 2705 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstYI r'GATC_y
Cuts at: 1972 2191 2292 2724 3238 3605 3990 4007 5459
Size: 219 101 432 514 367 385 17 1452
Cuts at: 5459 5545 5557 5903 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 514 432 385 367 346 219
101 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2796 2848 2895 3072 3169 513
Size: 552 957 774 52 47 177 97 3252
Fragments arranged by size:
3252 957 774 552 177 97 52 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5169
Fragments arranged by size:
5169 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 3424 4218 4238 2158
Size: 1266 794 20 3828
Fragments arranged by size:
3828 1266 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4915 809
Size: 507 3599 1802
Fragments arranged by size:
3599 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3115 3355 4244
Size: 54 1425 50 275 18 1285 240 889
Cuts at: 4244 5873 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1285 889 275 240 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3355 1537
Size: 275 1543 4090
Fragments arranged by size:
4090 1543 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2571 3838 1353
Size: 765 453 1267 3423
Fragments arranged by size:
3423 1267 765 453
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2585 3177 4495 4869 5015 387
Size: 1064 138 996 592 1318 374 146 1280
Fragments arranged by size:
1318 1280 1064 996 592 374 146 138
EcoICRI GAG'CTC
Cuts at: 2032 2704 2032
Size: 672 5236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2103 2151 2750 2780 2834 2990 3033
Size: 393 471 48 599 30 54 156 43
Cuts at: 3033 3971 4568 5355 5623 1239
Size: 938 597 787 268 1524
Fragments arranged by size:
1524 938 787 599 597 471 393 268
156 54 48 43 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2133 2225 2287 2133
Size: 92 62 5754
Fragments arranged by size:
5754 92 62
EcoO109I rG'GnC_Cy
Cuts at: 2876 2912 2990 2876
Size: 36 78 5794
Fragments arranged by size:
5794 78 36
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2600 2600
Size: 5908
FspI TGC'GCA
Cuts at: 3038 4414 3038
Size: 1376 4532
HaeII r_GCGC'y
Cuts at: 1678 2426 2848 2887 3148 5055 1678
Size: 748 422 39 261 1907 2531
Fragments arranged by size:
2531 1907 748 422 261 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2269 2301 4549 4581 4623
Size: 32 135 32 697 32 2248 32 42
Cuts at: 4623 4655 1373
Size: 32 2626
Fragments arranged by size:
2626 2248 697 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3252 3498 669
Size: 988 281 1314 246 3079
Fragments arranged by size:
3079 1314 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KasI G'GCGC_C
Cuts at: 2844 3144 2844
Size: 300 5608
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 5022 5206 1305
Size: 888 2829 184 2007
Fragments arranged by size:
2829 2007 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3866 4225 4384 518
Size: 648 2700 359 159 2042
Fragments arranged by size:
2700 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2263 2850 2940 3324 4001 5149 5394 1606
Size: 657 587 90 384 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 657 587 384 245 90
NarI GG'CG_CC
Cuts at: 2845 3145 2845
Size: 300 5608
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4399
Fragments arranged by size:
4399 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5908
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5908
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 2452 3266 4811 1132
Size: 97 1223 814 1545 2229
Fragments arranged by size:
2229 1545 1223 814 97
PasI CC'CwG_GG
Cuts at: 1348 2872 1348
Size: 1524 4384
PciI A'CATG_T
Cuts at: 4807 4807
Size: 5908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2728 2872 3124 1108
Size: 1620 144 252 3892
Fragments arranged by size:
3892 1620 252 144
PfoI T'CCnGG_A
Cuts at: 2044 2727 2044
Size: 683 5225
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PmlI CAC'GTG
Cuts at: 2526 2526
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 5490 5522 3977
Size: 32 1481 32 4363
Fragments arranged by size:
4363 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2912 2912
Size: 5908
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 3478 3478
Size: 5908
PspOMI G'GGCC_C
Cuts at: 2875 2990 2875
Size: 115 5793
PstI C_TGCA'G
Cuts at: 830 2658 3060 3260 830
Size: 1828 402 200 3478
Fragments arranged by size:
3478 1828 402 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5908
PvuII CAG'CTG
Cuts at: 2263 2263
Size: 5908
SacI G_AGCT'C
Cuts at: 2034 2706 2034
Size: 672 5236
SacII CC_GC'GG
Cuts at: 2851 2851
Size: 5908
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SapI GCTCTTCn'nnn_
Cuts at: 2118 2571 2118
Size: 453 5455
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 4156 5868 4156
Size: 1712 4196
SexAI A'CCwGG_T
Cuts at: 2247 2247
Size: 5908
SfcI C'TryA_G
Cuts at: 826 1045 2258 2297 2654 3056 3256 4391 5072
Size: 219 1213 39 357 402 200 1135 681
Cuts at: 5072 5263 826
Size: 191 1471
Fragments arranged by size:
1471 1213 1135 681 402 357 219 200
191 39
SfoI GGC'GCC
Cuts at: 2846 3146 2846
Size: 300 5608
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5908
SmaI CCC'GGG
Cuts at: 1872 3235 1872
Size: 1363 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2463 2705 4012 4913 5175 820
Size: 197 926 520 242 1307 901 262 1553
Fragments arranged by size:
1553 1307 926 901 520 262 242 197
SnaBI TAC'GTA
Cuts at: 493 3205 3699 493
Size: 2712 494 2702
Fragments arranged by size:
2712 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 2452 3266 1132
Size: 1320 814 3774
Fragments arranged by size:
3774 1320 814
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 3090 1517
Size: 1573 4335
StyI C'CwwG_G
Cuts at: 513 1065 2022 2105 2745 3347 513
Size: 552 957 83 640 602 3074
Fragments arranged by size:
3074 957 640 602 552 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3935 3952 4105 4290 4735 325
Size: 1255 2355 17 153 185 445 1498
Fragments arranged by size:
2355 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4154 5866 96
Size: 274 80 33 51 1443 2177 1712 138
Fragments arranged by size:
2177 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2510 2542 5868 5900 2510
Size: 32 3326 32 2518
Fragments arranged by size:
3326 2518 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3101 1316
Size: 1785 4123
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XhoI C'TCGA_G
Cuts at: 1943 2463 1943
Size: 520 5388
XmaI C'CCGG_G
Cuts at: 1870 3233 1870
Size: 1363 4545
XmnI GAAnn'nnTTC
Cuts at: 4037 4037
Size: 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3716 276
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI
SfiI SrfI SwaI XcmI