Restriction map for
pFN21ASDA1719
(Circular) (Six-base) MAPSORT of: pFN21ASDA1719.seq Check: 7508 from: 1 to: 7858
pFN21ASDA1719 7858 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3163 3520 4183 3163
Size: 357 663 6838
Fragments arranged by size:
6838 663 357
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2469 5668 278
Size: 53 83 186 936 933 3199 2468
Fragments arranged by size:
3199 2468 936 933 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5201 1937
Size: 3264 4594
Acc65I G'GTAC_C
Cuts at: 5179 5179
Size: 7858
AclI AA'CG_TT
Cuts at: 1563 4146 5985 6358 1563
Size: 2583 1839 373 3063
Fragments arranged by size:
3063 2583 1839 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2621 3947 4019 4238 5921 7305 7573 1632
Size: 989 1326 72 219 1683 1384 268 1917
Fragments arranged by size:
1917 1683 1384 1326 989 268 219 72
AfeI AGC'GCT
Cuts at: 4004 4004
Size: 7858
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7661
AflIII A'CryG_T
Cuts at: 1203 6757 7734 1203
Size: 5554 977 1327
Fragments arranged by size:
5554 1327 977
AgeI A'CCGG_T
Cuts at: 6689 6689
Size: 7858
AhdI GACnn_n'nnGTC
Cuts at: 4663 6587 4663
Size: 1924 5934
AleI CACnn'nnGTG
Cuts at: 1166 2165 1166
Size: 999 6859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7858
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 7826
AlwNI CAG_nnn'CTG
Cuts at: 2792 3604 4604 5601 7173 2792
Size: 812 1000 997 1572 3477
Fragments arranged by size:
3477 1572 1000 997 812
ApaI G_GGCC'C
Cuts at: 2073 4418 5116 2073
Size: 2345 698 4815
Fragments arranged by size:
4815 2345 698
ApaLI G'TGCA_C
Cuts at: 3371 5915 7071 3371
Size: 2544 1156 4158
Fragments arranged by size:
4158 2544 1156
ApoI r'AATT_y
Cuts at: 1493 5167 5393 5545 7654 1493
Size: 3674 226 152 2109 1697
Fragments arranged by size:
3674 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6412 160
Size: 872 5380 1606
Fragments arranged by size:
5380 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7858
AvaI C'yCGr_G
Cuts at: 1870 1943 4446 4614 4739 5183 1870
Size: 73 2503 168 125 444 4545
Fragments arranged by size:
4545 2503 444 168 125 73
BamHI G'GATC_C
Cuts at: 2325 3257 5188 5555 2325
Size: 932 1931 367 4628
Fragments arranged by size:
4628 1931 932 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2219 2516 2927 3683 3815
Size: 325 217 627 432 297 411 756 132
Cuts at: 3815 3974 4106 4718 5035 5179 6634 618
Size: 159 132 612 317 144 1455 1842
Fragments arranged by size:
1842 1455 756 627 612 432 411 325
317 297 217 159 144 132 132
BanII G_rGCy'C
Cuts at: 1391 2037 2073 2597 2893 3326 3768 4418 4512
Size: 646 36 524 296 433 442 650 94
Cuts at: 4512 4598 5116 5177 1391
Size: 86 518 61 4072
Fragments arranged by size:
4072 650 646 524 518 442 433 296
94 86 61 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3177 4662 928
Size: 971 1278 1485 4124
Fragments arranged by size:
4124 1485 1278 971
BbvCI CC'TCA_GC
Cuts at: 3413 4484 4688 3413
Size: 1071 204 6583
Fragments arranged by size:
6583 1071 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6049 6083 1903
Size: 34 4112 34 3678
Fragments arranged by size:
4112 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5750 6960 5750
Size: 1210 6648
BclI T'GATC_A
Cuts at: 1548 2175 3771 5073 1548
Size: 627 1596 1302 4333
Fragments arranged by size:
4333 1596 1302 627
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3037 3361 3620
Size: 107 122 71 631 605 1365 324 259
Cuts at: 3620 4158 4480 6469 136
Size: 538 322 1989 1525
Fragments arranged by size:
1989 1525 1365 631 605 538 324 322
259 122 107 71
BglII A'GATC_T
Cuts at: 7853 7853
Size: 7858
BlpI GC'TnA_GC
Cuts at: 2036 2621 4169 5275 2036
Size: 585 1548 1106 4619
Fragments arranged by size:
4619 1548 1106 585
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2521 2535 3018 3102 3375 3818 4040
Size: 283 448 14 483 84 273 443 222
Cuts at: 4040 4356 4418 4454 4721 5116 5919 7075 1790
Size: 316 62 36 267 395 803 1156 2573
Fragments arranged by size:
2573 1156 803 483 448 443 395 316
283 273 267 222 84 62 36 14
BmgBI CAC'GTC
Cuts at: 1318 5002 1318
Size: 3684 4174
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4350 5589 6547
Size: 416 53 83 399 24 2933 1239 958
Cuts at: 6547 442
Size: 1753
Fragments arranged by size:
2933 1753 1239 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3338 1056
Size: 2282 5576
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3967 4667 3428
Size: 539 700 6619
Fragments arranged by size:
6619 700 539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2073 2417 2922 3020 3325 3413 3419 3659
Size: 834 344 505 98 305 88 6 240
Cuts at: 3659 3842 3983 4221 4682 4718 4952 6518 1239
Size: 183 141 238 461 36 234 1566 2579
Fragments arranged by size:
2579 1566 834 505 461 344 305 240
238 234 183 141 98 88 36 6
Bpu10I CC'TnA_GC
Cuts at: 3413 4484 4688 3413
Size: 1071 204 6583
Fragments arranged by size:
6583 1071 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2975 4139 5983 6848 7146 2354
Size: 621 1164 1844 865 298 3066
Fragments arranged by size:
3066 1844 1164 865 621 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2039 2251 2898 3374 3399 3452 3959
Size: 617 540 212 647 476 25 53 507
Cuts at: 3959 4199 4271 6521 882
Size: 240 72 2250 2219
Fragments arranged by size:
2250 2219 647 617 540 507 476 240
212 72 53 25
BsaAI yAC'GTr
Cuts at: 493 1204 2563 3694 4117 5155 5649 493
Size: 711 1359 1131 423 1038 494 2702
Fragments arranged by size:
2702 1359 1131 1038 711 494 423
BsaBI GATnn'nnATC
Cuts at: 7852 7852
Size: 7858
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2466 2783 3612 3885
Size: 53 83 186 936 933 317 829 273
Cuts at: 3885 5665 6047 6682 275
Size: 1780 382 635 1451
Fragments arranged by size:
1780 1451 936 933 829 635 382 317
273 186 83 53
BsaWI w'CCGG_w
Cuts at: 2826 3933 6291 6689 6963 7110 7521 2826
Size: 1107 2358 398 274 147 411 3163
Fragments arranged by size:
3163 2358 1107 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2390 2420 3632 3662 4194
Size: 30 165 30 987 30 1212 30 532
Cuts at: 4194 4224 4799 4829 1178
Size: 30 575 30 4207
Fragments arranged by size:
4207 1212 987 575 532 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2526 3079 3310 4574 4577 4580 4634
Size: 973 367 553 231 1264 3 3 54
Cuts at: 4634 4823 1186
Size: 189 4221
Fragments arranged by size:
4221 1264 973 553 367 231 189 54
3 3
BseYI C'CCAG_C
Cuts at: 2197 3084 3239 3261 3724 4300 4537 4597 5132
Size: 887 155 22 463 576 237 60 535
Cuts at: 5132 7061 7557 2197
Size: 1929 496 2498
Fragments arranged by size:
2498 1929 887 576 535 496 463 237
155 60 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2627 2707 2879 3062 3080 4745 4838
Size: 573 891 80 172 183 18 1665 93
Cuts at: 4838 1163
Size: 4183
Fragments arranged by size:
4183 1665 891 573 183 172 93 80
18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 4166 5123 5308 6069 7097
Size: 275 186 853 1312 957 185 761 1028
Cuts at: 7097 1540
Size: 2301
Fragments arranged by size:
2301 1312 1028 957 853 761 275 186
185
BsiHKAI G_wGCw'C
Cuts at: 3326 3375 3399 5919 6004 7075 3326
Size: 49 24 2520 85 1071 4109
Fragments arranged by size:
4109 2520 1071 85 49 24
BsmI GAATG_Cn'
Cuts at: 2136 4128 5369 5462 2136
Size: 1992 1241 93 4532
Fragments arranged by size:
4532 1992 1241 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2473 4897 2473
Size: 2424 5434
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2073 2521 2535 2597 2893 3018
Size: 399 247 36 448 14 62 296 125
Cuts at: 3018 3102 3326 3375 3399 3768 3818 4040 4356
Size: 84 224 49 24 369 50 222 316
Cuts at: 4356 4418 4454 4512 4598 4721 5116 5177 5919
Size: 62 36 58 86 123 395 61 742
Cuts at: 5919 6004 7075 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 448 399 395 369 316
296 247 224 222 125 123 86 85
84 62 62 61 58 50 49 36
36 24 14
BspEI T'CCGG_A
Cuts at: 2826 3933 7521 2826
Size: 1107 3588 3163
Fragments arranged by size:
3588 3163 1107
BspHI T'CATG_A
Cuts at: 4710 5747 7477 4710
Size: 1037 1730 5091
Fragments arranged by size:
5091 1730 1037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3163 3520 4183 4981 5213 844
Size: 2319 357 663 798 232 3489
Fragments arranged by size:
3489 2319 798 663 357 232
BsrBI CCG'CTC
Cuts at: 1394 1823 2426 2886 4468 5745 1394
Size: 429 603 460 1582 1277 3507
Fragments arranged by size:
3507 1582 1277 603 460 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3561 6353 6527 65
Size: 1183 2313 2792 174 1396
Fragments arranged by size:
2792 2313 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4161 4345 4758 6502 6689 1700
Size: 110 2351 184 413 1744 187 2869
Fragments arranged by size:
2869 2351 1744 413 187 184 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7858
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7858
BssSI C'ACGA_G
Cuts at: 2693 3479 5918 6930 2693
Size: 786 2439 1012 3621
Fragments arranged by size:
3621 2439 1012 786
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3370 3898 794
Size: 2576 528 4754
Fragments arranged by size:
4754 2576 528
BstBI TT'CG_AA
Cuts at: 2007 6662 2007
Size: 4655 3203
BstEII G'GTnAC_C
Cuts at: 1184 4846 1184
Size: 3662 4196
BstXI CCAn_nnnn'nTGG
Cuts at: 2528 2668 2875 2971 3349 4117 2528
Size: 140 207 96 378 768 6269
Fragments arranged by size:
6269 768 378 207 140 96
BstYI r'GATC_y
Cuts at: 1972 2325 2802 3003 3257 3930 5188 5555 5940
Size: 353 477 201 254 673 1258 367 385
Cuts at: 5940 5957 7409 7495 7507 7853 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1258 673 477 385 367 353
346 254 201 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3253 3384 3876 4957 513
Size: 552 2188 131 492 1081 3414
Fragments arranged by size:
3414 2188 1081 552 492 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7119
Fragments arranged by size:
7119 683 56
BtsI GCAGTG_nn'
Cuts at: 2690 2941 3057 3127 3685 3911 5374 6168 6188
Size: 251 116 70 558 226 1463 794 20
Cuts at: 6188 2690
Size: 4360
Fragments arranged by size:
4360 1463 794 558 251 226 116 70
20
ClaI AT'CG_AT
Cuts at: 1554 2922 1554
Size: 1368 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2442 2477 4745 4780 529
Size: 35 1878 35 2268 35 3607
Fragments arranged by size:
3607 2268 1878 35 35 35
DraI TTT'AAA
Cuts at: 5162 5162
Size: 7858
DraIII CAC_nnn'GTG
Cuts at: 4846 4933 4846
Size: 87 7771
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2788 4061 6865 809
Size: 507 1472 1273 2804 1802
Fragments arranged by size:
2804 1802 1472 1273 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2670 2724 2973
Size: 54 1425 50 275 18 840 54 249
Cuts at: 2973 3029 3124 3160 3744 3843 4134 4163 4392
Size: 56 95 36 584 99 291 29 229
Cuts at: 4392 4753 5046 5305 6194 7823 8
Size: 361 293 259 889 1629 43
Fragments arranged by size:
1629 1425 889 840 584 361 293 291
275 259 249 229 99 95 56 54
54 50 43 36 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 4163 5305 1537
Size: 275 2351 1142 4090
Fragments arranged by size:
4090 2351 1142 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5788 1353
Size: 4435 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2075 2333 3307 3674 3848 6445
Size: 1064 138 486 258 974 367 174 2597
Cuts at: 6445 6819 6965 387
Size: 374 146 1280
Fragments arranged by size:
2597 1280 1064 974 486 374 367 258
174 146 138
EcoICRI GAG'CTC
Cuts at: 3324 3324
Size: 7858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2417 2621 2922 3020 3325 3413
Size: 393 441 344 204 301 98 305 88
Cuts at: 3413 3419 3659 3842 3947 3983 4019 4221 4238
Size: 6 240 183 105 36 36 202 17
Cuts at: 4238 4682 4718 4952 5921 6518 7305 7573 1239
Size: 444 36 234 969 597 787 268 1524
Fragments arranged by size:
1524 969 787 597 444 441 393 344
305 301 268 240 234 204 202 183
105 98 88 36 36 36 17 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2477 4190 4856 4978 5048 2477
Size: 1713 666 122 70 5287
Fragments arranged by size:
5287 1713 666 122 70
EcoO109I rG'GnC_Cy
Cuts at: 2070 2756 3039 3088 3913 4013 4297 4414 4422
Size: 686 283 49 825 100 284 117 8
Cuts at: 4422 4861 4984 5113 5129 2070
Size: 439 123 129 16 4799
Fragments arranged by size:
4799 825 686 439 284 283 129 123
117 100 49 16 8
EcoRI G'AATT_C
Cuts at: 5167 5545 5167
Size: 378 7480
FseI GG_CCGG'CC
Cuts at: 4165 4165
Size: 7858
FspI TGC'GCA
Cuts at: 6364 6364
Size: 7858
HaeII r_GCGC'y
Cuts at: 1678 4006 7005 1678
Size: 2328 2999 2531
Fragments arranged by size:
2999 2531 2328
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2895 2927 3449 3481 4194
Size: 32 135 32 1323 32 522 32 713
Cuts at: 4194 4226 4649 4681 6499 6531 6573 6605 1373
Size: 32 423 32 1818 32 42 32 2626
Fragments arranged by size:
2626 1818 1323 713 522 423 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4996 5202 5448 669
Size: 988 281 3058 206 246 3079
Fragments arranged by size:
3079 3058 988 281 246 206
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7858
HpaI GTT'AAC
Cuts at: 5448 5448
Size: 7858
KpnI G_GTAC'C
Cuts at: 5183 5183
Size: 7858
MfeI C'AATT_G
Cuts at: 5457 5457
Size: 7858
MluI A'CGCG_T
Cuts at: 7734 7734
Size: 7858
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 3804 6972 7156 1305
Size: 969 1530 3168 184 2007
Fragments arranged by size:
3168 2007 1530 969 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2672 2726 2975 3162 3845
Size: 54 1425 343 840 54 249 187 683
Cuts at: 3845 4136 4755 10
Size: 291 619 3113
Fragments arranged by size:
3113 1425 840 683 619 343 291 249
187 54 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2526 2933 4115 5816 6175 6334
Size: 648 999 361 407 1182 1701 359 159
Cuts at: 6334 518
Size: 2042
Fragments arranged by size:
2042 1701 1182 999 648 407 361 359
159
MspA1I CmG'CkG
Cuts at: 1606 2641 3028 3076 3084 3417 4168 4541 5274
Size: 1035 387 48 8 333 751 373 733
Cuts at: 5274 5951 7099 7344 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1035 751 733 677 387 373
333 245 48 8
NaeI GCC'GGC
Cuts at: 4163 4347 4163
Size: 184 7674
NcoI C'CATG_G
Cuts at: 513 1065 3876 513
Size: 552 2811 4495
Fragments arranged by size:
4495 2811 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7858
NgoMIV G'CCGG_C
Cuts at: 4161 4345 4161
Size: 184 7674
NheI G'CTAG_C
Cuts at: 1052 3334 1052
Size: 2282 5576
NotI GC'GGCC_GC
Cuts at: 5305 5305
Size: 7858
NspI r_CATG'y
Cuts at: 1132 1229 4639 5216 6761 1132
Size: 97 3410 577 1545 2229
Fragments arranged by size:
3410 2229 1545 577 97
PasI CC'CwG_GG
Cuts at: 1348 2241 2797 4018 4418 1348
Size: 893 556 1221 400 4788
Fragments arranged by size:
4788 1221 893 556 400
PciI A'CATG_T
Cuts at: 6757 6757
Size: 7858
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3680 4604 1108
Size: 2572 924 4362
Fragments arranged by size:
4362 2572 924
PfoI T'CCnGG_A
Cuts at: 4905 4905
Size: 7858
PmeI GTTT'AAAC
Cuts at: 5162 5162
Size: 7858
PmlI CAC'GTG
Cuts at: 3694 4117 3694
Size: 423 7435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 5927 5959 7440 7472 3632
Size: 32 2263 32 1481 32 4018
Fragments arranged by size:
4018 2263 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2756 3088 3913 4013 4297 4422 4861 4984 2756
Size: 332 825 100 284 125 439 123 5630
Fragments arranged by size:
5630 825 439 332 284 125 123 100
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7858
PsiI TTA'TAA
Cuts at: 5428 5428
Size: 7858
PspOMI G'GGCC_C
Cuts at: 2069 4414 5112 2069
Size: 2345 698 4815
Fragments arranged by size:
4815 2345 698
PspXI vC'TCGA_Gb
Cuts at: 4739 4739
Size: 7858
PstI C_TGCA'G
Cuts at: 830 3134 3531 4194 4801 4992 5210 830
Size: 2304 397 663 607 191 218 3478
Fragments arranged by size:
3478 2304 663 607 397 218 191
PvuI CG_AT'CG
Cuts at: 2001 5123 2001
Size: 3122 4736
PvuII CAG'CTG
Cuts at: 3076 4541 3076
Size: 1465 6393
SacI G_AGCT'C
Cuts at: 3326 3326
Size: 7858
SalI G'TCGA_C
Cuts at: 1936 5200 1936
Size: 3264 4594
SanDI GG'GwC_CC
Cuts at: 2756 3088 4013 2756
Size: 332 925 6601
Fragments arranged by size:
6601 925 332
SbfI CC_TGCA'GG
Cuts at: 4194 4992 5210 4194
Size: 798 218 6842
Fragments arranged by size:
6842 798 218
ScaI AGT'ACT
Cuts at: 6106 7818 6106
Size: 1712 6146
SexAI A'CCwGG_T
Cuts at: 3915 3915
Size: 7858
SfcI C'TryA_G
Cuts at: 826 1045 3130 3527 4190 4797 4988 5206 6341
Size: 219 2085 397 663 607 191 218 1135
Cuts at: 6341 7022 7213 826
Size: 681 191 1471
Fragments arranged by size:
2085 1471 1135 681 663 607 397 219
218 191 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3037 3037
Size: 7858
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7858
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7858
SmaI CCC'GGG
Cuts at: 1872 4448 5185 1872
Size: 2576 737 4545
Fragments arranged by size:
4545 2576 737
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2369 2954 4154 4739 5962 6863
Size: 197 926 426 585 1200 585 1223 901
Cuts at: 6863 7125 820
Size: 262 1553
Fragments arranged by size:
1553 1223 1200 926 901 585 585 426
262 197
SnaBI TAC'GTA
Cuts at: 493 5155 5649 493
Size: 4662 494 2702
Fragments arranged by size:
4662 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7858
SphI G_CATG'C
Cuts at: 1132 5216 1132
Size: 4084 3774
SspI AAT'ATT
Cuts at: 5 52 5782 5
Size: 47 5730 2081
Fragments arranged by size:
5730 2081 47
StuI AGG'CCT
Cuts at: 1517 4074 4323 4474 4750 4965 1517
Size: 2557 249 151 276 215 4410
Fragments arranged by size:
4410 2557 276 249 215 151
StyI C'CwwG_G
Cuts at: 513 1065 3876 4763 4830 5297 513
Size: 552 2811 887 67 467 3074
Fragments arranged by size:
3074 2811 887 552 467 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2301 4516 5885 5902 6055 6240 6685
Size: 1255 721 2215 1369 17 153 185 445
Cuts at: 6685 325
Size: 1498
Fragments arranged by size:
2215 1498 1369 1255 721 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6104 7816 96
Size: 274 80 33 51 1443 4127 1712 138
Fragments arranged by size:
4127 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2900 2932 3209 3241 7818 7850 2900
Size: 32 277 32 4577 32 2908
Fragments arranged by size:
4577 2908 277 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3463 3542 4982 1316
Size: 2147 79 1440 4192
Fragments arranged by size:
4192 2147 1440 79
XbaI T'CTAG_A
Cuts at: 5194 5194
Size: 7858
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2968 4087 2968
Size: 1119 6739
XhoI C'TCGA_G
Cuts at: 1943 4739 1943
Size: 2796 5062
XmaI C'CCGG_G
Cuts at: 1870 4446 5183 1870
Size: 2576 737 4545
Fragments arranged by size:
4545 2576 737
XmnI GAAnn'nnTTC
Cuts at: 5987 5987
Size: 7858
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2467 5666 276
Size: 53 83 186 936 933 3199 2468
Fragments arranged by size:
3199 2468 936 933 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PsrI RsrII SacII SapI SfoI SrfI SwaI