Restriction map for
pFN21ASDA1712
(Circular) (Six-base) MAPSORT of: pFN21ASDA1712.seq Check: 9967 from: 1 to: 5899
pFN21ASDA1712 5899 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 1937
Size: 1305 4594
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2112 2384 2685 3962 5346 5614 1632
Size: 480 272 301 1277 1384 268 1917
Fragments arranged by size:
1917 1384 1277 480 301 272 268
AflII C'TTAA_G
Cuts at: 820 1017 2765 820
Size: 197 1748 3954
Fragments arranged by size:
3954 1748 197
AflIII A'CryG_T
Cuts at: 1203 4798 5775 1203
Size: 3595 977 1327
Fragments arranged by size:
3595 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2055 2087 2055
Size: 32 5867
AlwNI CAG_nnn'CTG
Cuts at: 2486 3642 5214 2486
Size: 1156 1572 3171
Fragments arranged by size:
3171 1572 1156
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 2915 3208 3434 3586 5695 1493
Size: 1422 293 226 152 2109 1697
Fragments arranged by size:
2109 1697 1422 293 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 3224 1870
Size: 73 1281 4545
Fragments arranged by size:
4545 1281 73
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3220 4675 618
Size: 325 217 627 1433 1455 1842
Fragments arranged by size:
1842 1455 1433 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2217 3218 1391
Size: 826 1001 4072
Fragments arranged by size:
4072 1001 826
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2390 2949 928
Size: 971 491 559 3878
Fragments arranged by size:
3878 971 559 491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2581 3791 5001 2581
Size: 1210 1210 3479
Fragments arranged by size:
3479 1210 1210
BclI T'GATC_A
Cuts at: 1548 2269 1548
Size: 721 5178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4510 136
Size: 107 122 71 631 605 2838 1525
Fragments arranged by size:
2838 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 2691 3316 2691
Size: 625 5274
Bme1580I G_kGCm'C
Cuts at: 1790 3960 5116 1790
Size: 2170 1156 2573
Fragments arranged by size:
2573 2170 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5899
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3630 4588 442
Size: 416 53 83 399 24 2213 958 1753
Fragments arranged by size:
2213 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4559 1239
Size: 3320 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4024 4889 5187 4024
Size: 865 298 4736
Fragments arranged by size:
4736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2118 4562 882
Size: 617 619 2444 2219
Fragments arranged by size:
2444 2219 619 617
BsaAI yAC'GTr
Cuts at: 493 1204 3196 3690 493
Size: 711 1992 494 2702
Fragments arranged by size:
2702 1992 711 494
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3706 4088 4723 275
Size: 53 83 186 936 2173 382 635 1451
Fragments arranged by size:
2173 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4332 4730 5004 5151 5562 4332
Size: 398 274 147 411 4669
Fragments arranged by size:
4669 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2055 2085 1178
Size: 30 165 30 652 30 4992
Fragments arranged by size:
4992 652 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5899
BseYI C'CCAG_C
Cuts at: 2128 5102 5598 2128
Size: 2974 496 2429
Fragments arranged by size:
2974 2429 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2712 1163
Size: 573 976 4350
Fragments arranged by size:
4350 976 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3349 4110 5138 1540
Size: 275 186 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2217 3960 4045 5116 2073
Size: 144 1743 85 1071 2856
Fragments arranged by size:
2856 1743 1071 144 85
BsmI GAATG_Cn'
Cuts at: 2756 3410 3503 2756
Size: 654 93 5152
Fragments arranged by size:
5152 654 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2217 3218 3960 4045 5116 1391
Size: 399 283 144 1001 742 85 1071 2174
Fragments arranged by size:
2174 1071 1001 742 399 283 144 85
BspEI T'CCGG_A
Cuts at: 5562 5562
Size: 5899
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2499 3254 844
Size: 1655 755 3489
Fragments arranged by size:
3489 1655 755
BsrBI CCG'CTC
Cuts at: 1394 1823 3786 1394
Size: 429 1963 3507
Fragments arranged by size:
3507 1963 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2470 4394 4568 65
Size: 1183 1222 1924 174 1396
Fragments arranged by size:
1924 1396 1222 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4543 4730 1700
Size: 110 2733 187 2869
Fragments arranged by size:
2869 2733 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5899
BssSI C'ACGA_G
Cuts at: 3959 4971 3959
Size: 1012 4887
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5899
BstBI TT'CG_AA
Cuts at: 2007 4703 2007
Size: 2696 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894
Size: 1257 367 385 17 1452 86 12 346
Cuts at: 5894 1972
Size: 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2521 2521
Size: 5899
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4390
Fragments arranged by size:
4390 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5160
Fragments arranged by size:
5160 683 56
BtsI GCAGTG_nn'
Cuts at: 3415 4209 4229 3415
Size: 794 20 5085
Fragments arranged by size:
5085 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3346 4235 5864
Size: 54 1425 50 275 18 1516 889 1629
Cuts at: 5864 8
Size: 43
Fragments arranged by size:
1629 1516 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3346 1537
Size: 275 1534 4090
Fragments arranged by size:
4090 1534 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 2880 3829 1353
Size: 735 792 949 3423
Fragments arranged by size:
3423 949 792 735
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4486 4860 5006 387
Size: 1064 138 2897 374 146 1280
Fragments arranged by size:
2897 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 2215
Size: 5899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2384 2685 3962 4559 5346 5614
Size: 393 480 272 301 1277 597 787 268
Cuts at: 5614 1239
Size: 1524
Fragments arranged by size:
1524 1277 787 597 480 393 301 272
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2063 2063
Size: 5899
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
EcoRV GAT'ATC
Cuts at: 2500 2500
Size: 5899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2780 2780
Size: 5899
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 5046 1678
Size: 3368 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 4540 4572 4614
Size: 32 135 32 887 32 2049 32 42
Cuts at: 4614 4646 1373
Size: 32 2626
Fragments arranged by size:
2626 2049 887 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2575 3243 3489 669
Size: 988 281 637 668 246 3079
Fragments arranged by size:
3079 988 668 637 281 246
HindIII A'AGCT_T
Cuts at: 748 2047 2258 748
Size: 1299 211 4389
Fragments arranged by size:
4389 1299 211
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5013 5197 1305
Size: 3708 184 2007
Fragments arranged by size:
3708 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4077
Fragments arranged by size:
4077 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3857 4216 4375 518
Size: 648 2691 359 159 2042
Fragments arranged by size:
2691 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3315 3992 5140 5385 1606
Size: 1709 677 1148 245 2120
Fragments arranged by size:
2120 1709 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4390
Fragments arranged by size:
4390 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 3257 4802 1132
Size: 97 2028 1545 2229
Fragments arranged by size:
2229 2028 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5899
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3127 1108
Size: 2019 3880
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2055 2087 3968 4000 5481 5513 2055
Size: 32 1881 32 1481 32 2441
Fragments arranged by size:
2441 1881 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 2195 2283 3469 2195
Size: 88 1186 4625
Fragments arranged by size:
4625 1186 88
PstI C_TGCA'G
Cuts at: 830 2482 3163 3251 830
Size: 1652 681 88 3478
Fragments arranged by size:
3478 1652 681 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
SacI G_AGCT'C
Cuts at: 2217 2217
Size: 5899
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SfcI C'TryA_G
Cuts at: 826 1045 2478 3034 3159 3247 4382 5063 5254
Size: 219 1433 556 125 88 1135 681 191
Cuts at: 5254 826
Size: 1471
Fragments arranged by size:
1471 1433 1135 681 556 219 191 125
88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 3226 1872
Size: 1354 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2765 4003 4904 5166 820
Size: 197 926 822 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 822 262 197
SnaBI TAC'GTA
Cuts at: 493 3196 3690 493
Size: 2703 494 2702
Fragments arranged by size:
2703 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5899
StyI C'CwwG_G
Cuts at: 513 1065 2022 3338 513
Size: 552 957 1316 3074
Fragments arranged by size:
3074 1316 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3926 3943 4096 4281 4726 325
Size: 1255 2346 17 153 185 445 1498
Fragments arranged by size:
2346 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3038 4145 5857
Size: 274 80 33 51 1443 1061 1107 1712
Cuts at: 5857 96
Size: 138
Fragments arranged by size:
1712 1443 1107 1061 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5859 5891 5859
Size: 32 5867
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 3224 1870
Size: 1354 4545
XmnI GAAnn'nnTTC
Cuts at: 4028 4028
Size: 5899
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI