Restriction map for
pFN21ASDA1709
(Circular) (Six-base) MAPSORT of: pFN21ASDA1709.seq Check: 7070 from: 1 to: 7804
pFN21ASDA1709 7804 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3685 3685
Size: 7804
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5614 278
Size: 53 83 186 936 4078 2468
Fragments arranged by size:
4078 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5147 1937
Size: 3210 4594
Acc65I G'GTAC_C
Cuts at: 5125 5125
Size: 7804
AclI AA'CG_TT
Cuts at: 1563 5931 6304 1563
Size: 4368 373 3063
Fragments arranged by size:
4368 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2166 3549 3609 3807 4289 4508 4907 5105
Size: 534 1383 60 198 482 219 399 198
Cuts at: 5105 5867 7251 7519 1632
Size: 762 1384 268 1917
Fragments arranged by size:
1917 1384 1383 762 534 482 399 268
219 198 198 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7607
AflIII A'CryG_T
Cuts at: 1203 2148 6703 7680 1203
Size: 945 4555 977 1327
Fragments arranged by size:
4555 1327 977 945
AgeI A'CCGG_T
Cuts at: 6635 6635
Size: 7804
AhdI GACnn_n'nnGTC
Cuts at: 6533 6533
Size: 7804
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7804
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 1184
Size: 1218 6586
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3885 3917 3885
Size: 32 7772
AlwNI CAG_nnn'CTG
Cuts at: 3149 3964 5547 7119 3149
Size: 815 1583 1572 3834
Fragments arranged by size:
3834 1583 1572 815
ApaLI G'TGCA_C
Cuts at: 5861 7017 5861
Size: 1156 6648
ApoI r'AATT_y
Cuts at: 1493 2819 2993 3071 4029 4850 5113 5339 5491
Size: 1326 174 78 958 821 263 226 152
Cuts at: 5491 7600 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1326 958 821 263 226 174
152 78
AseI AT'TA_AT
Cuts at: 160 1032 6358 160
Size: 872 5326 1606
Fragments arranged by size:
5326 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7804
AvaI C'yCGr_G
Cuts at: 1870 1943 2877 3807 4684 5129 1870
Size: 73 934 930 877 445 4545
Fragments arranged by size:
4545 934 930 877 445 73
BamHI G'GATC_C
Cuts at: 5134 5501 5134
Size: 367 7437
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5125 6580 618
Size: 325 217 627 3338 1455 1842
Fragments arranged by size:
3338 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4806 5123 1391
Size: 3415 317 4072
Fragments arranged by size:
4072 3415 317
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4277 4820 928
Size: 971 2378 543 3912
Fragments arranged by size:
3912 2378 971 543
BbvCI CC'TCA_GC
Cuts at: 2726 2726
Size: 7804
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2356 2390 3659 3693 5995 6029 1903
Size: 34 419 34 1269 34 2302 34 3678
Fragments arranged by size:
3678 2302 1269 419 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5696 6906 5696
Size: 1210 6594
BclI T'GATC_A
Cuts at: 1548 3087 1548
Size: 1539 6265
BfrBI ATG'CAT
Cuts at: 2026 2026
Size: 7804
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3228 3383 6415
Size: 107 122 71 631 605 1556 155 3032
Cuts at: 6415 136
Size: 1525
Fragments arranged by size:
3032 1556 1525 631 605 155 122 107
71
BglII A'GATC_T
Cuts at: 2090 7799 2090
Size: 5709 2095
BlpI GC'TnA_GC
Cuts at: 2079 5221 2079
Size: 3142 4662
Bme1580I G_kGCm'C
Cuts at: 1790 3278 3714 5865 7021 1790
Size: 1488 436 2151 1156 2573
Fragments arranged by size:
2573 2151 1488 1156 436
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7804
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5535 6493 442
Size: 416 53 83 399 24 4118 958 1753
Fragments arranged by size:
4118 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7804
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2851 3581 4699 2851
Size: 730 1118 5956
Fragments arranged by size:
5956 1118 730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2741 3401 3641 4019 4517 6464 1239
Size: 1502 660 240 378 498 1947 2579
Fragments arranged by size:
2579 1947 1502 660 498 378 240
Bpu10I CC'TnA_GC
Cuts at: 2726 4445 2726
Size: 1719 6085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 3281 4034 4166 5929 6794 7092 2477
Size: 804 753 132 1763 865 298 3189
Fragments arranged by size:
3189 1763 865 804 753 298 132
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2342 6467 882
Size: 617 843 4125 2219
Fragments arranged by size:
4125 2219 843 617
BsaAI yAC'GTr
Cuts at: 493 1204 3622 5101 5595 493
Size: 711 2418 1479 494 2702
Fragments arranged by size:
2702 2418 1479 711 494
BsaBI GATnn'nnATC
Cuts at: 3385 7798 3385
Size: 4413 3391
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5611 5993 6628 275
Size: 53 83 186 936 4078 382 635 1451
Fragments arranged by size:
4078 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2052 2226 2692 4244 4710 6237 6635 6909 7056
Size: 174 466 1552 466 1527 398 274 147
Cuts at: 7056 7467 2052
Size: 411 2389
Fragments arranged by size:
2389 1552 1527 466 466 411 398 274
174 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4366 4396 4458 4488 1178
Size: 30 165 30 2963 30 62 30 4494
Fragments arranged by size:
4494 2963 165 62 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2641 2866 4097 4169 4469 4781 1186
Size: 1455 225 1231 72 300 312 4209
Fragments arranged by size:
4209 1455 1231 312 300 225 72
BseYI C'CCAG_C
Cuts at: 2209 2757 2918 3157 4434 4582 4958 7007 7503
Size: 548 161 239 1277 148 376 2049 496
Cuts at: 7503 2209
Size: 2510
Fragments arranged by size:
2510 2049 1277 548 496 376 239 161
148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5254 6015 7043 1540
Size: 275 186 3253 761 1028 2301
Fragments arranged by size:
3253 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3955 4254 5865 5950 7021 3955
Size: 299 1611 85 1071 4738
Fragments arranged by size:
4738 1611 1071 299 85
BsmI GAATG_Cn'
Cuts at: 2244 2524 3730 5315 5408 2244
Size: 280 1206 1585 93 4640
Fragments arranged by size:
4640 1585 1206 280 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3278 3714 3955 4254 4806 5123 5865
Size: 399 1488 436 241 299 552 317 742
Cuts at: 5865 5950 7021 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1488 1071 742 552 436 399 317
299 241 85
BspEI T'CCGG_A
Cuts at: 2052 2692 7467 2052
Size: 640 4775 2389
Fragments arranged by size:
4775 2389 640
BspHI T'CATG_A
Cuts at: 5693 7423 5693
Size: 1730 6074
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3685 3975 4101 5159 844
Size: 2841 290 126 1058 3489
Fragments arranged by size:
3489 2841 1058 290 126
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 5691 1394
Size: 429 260 3608 3507
Fragments arranged by size:
3608 3507 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 3906 6299 6473 65
Size: 1183 2658 2393 174 1396
Fragments arranged by size:
2658 2393 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3282 6448 6635 1700
Size: 110 1472 3166 187 2869
Fragments arranged by size:
3166 2869 1472 187 110
BsrGI T'GTAC_A
Cuts at: 96 2502 96
Size: 2406 5398
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7804
BssSI C'ACGA_G
Cuts at: 4255 5864 6876 4255
Size: 1609 1012 5183
Fragments arranged by size:
5183 1609 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3964 794
Size: 3170 4634
BstBI TT'CG_AA
Cuts at: 2007 6608 2007
Size: 4601 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7804
BstYI r'GATC_y
Cuts at: 1972 2090 2358 3865 5134 5501 5886 5903 7355
Size: 118 268 1507 1269 367 385 17 1452
Cuts at: 7355 7441 7453 7799 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1507 1452 1269 385 367 346 268
118 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2849 2849
Size: 7804
BtgI C'CryG_G
Cuts at: 513 1065 2667 3839 4918 513
Size: 552 1602 1172 1079 3399
Fragments arranged by size:
3399 1602 1172 1079 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7065
Fragments arranged by size:
7065 683 56
BtsI GCAGTG_nn'
Cuts at: 3090 3348 3398 3456 5320 6114 6134 3090
Size: 258 50 58 1864 794 20 4760
Fragments arranged by size:
4760 1864 794 258 58 50 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7804
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7769
DraI TTT'AAA
Cuts at: 3004 5108 3004
Size: 2104 5700
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6811 809
Size: 507 5495 1802
Fragments arranged by size:
5495 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2424 3280 4551
Size: 54 1425 50 275 18 594 856 1271
Cuts at: 4551 5251 6140 7769 8
Size: 700 889 1629 43
Fragments arranged by size:
1629 1425 1271 889 856 700 594 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5251 1537
Size: 275 3439 4090
Fragments arranged by size:
4090 3439 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 3212 3695 4062 4475 4623 4883 4926
Size: 959 900 483 367 413 148 260 43
Cuts at: 4926 5734 1353
Size: 808 3423
Fragments arranged by size:
3423 959 900 808 483 413 367 260
148 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6391 6765 6911 387
Size: 1064 138 4802 374 146 1280
Fragments arranged by size:
4802 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2741 3401 3549 3609 3641 3807
Size: 393 534 575 660 148 60 32 166
Cuts at: 3807 4019 4289 4508 4517 4907 5105 5867 6464
Size: 212 270 219 9 390 198 762 597
Cuts at: 6464 7251 7519 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 762 660 597 575 534 393
390 270 268 219 212 198 166 148
60 32 9
EcoO109I rG'GnC_Cy
Cuts at: 2674 3829 4009 4090 4588 2674
Size: 1155 180 81 498 5890
Fragments arranged by size:
5890 1155 498 180 81
EcoRI G'AATT_C
Cuts at: 5113 5491 5113
Size: 378 7426
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 7804
FspI TGC'GCA
Cuts at: 6310 6310
Size: 7804
HaeII r_GCGC'y
Cuts at: 1678 3330 6951 1678
Size: 1652 3621 2531
Fragments arranged by size:
3621 2531 1652
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2692 2724 6445 6477 6519
Size: 32 135 32 1120 32 3721 32 42
Cuts at: 6519 6551 1373
Size: 32 2626
Fragments arranged by size:
3721 2626 1120 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3320 5148 5394 669
Size: 988 281 1382 1828 246 3079
Fragments arranged by size:
3079 1828 1382 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3531 748
Size: 2783 5021
HpaI GTT'AAC
Cuts at: 5394 5394
Size: 7804
KpnI G_GTAC'C
Cuts at: 5129 5129
Size: 7804
MfeI C'AATT_G
Cuts at: 2498 5403 2498
Size: 2905 4899
MluI A'CGCG_T
Cuts at: 7680 7680
Size: 7804
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3625 4044 4360 6918 7102 1305
Size: 2320 419 316 2558 184 2007
Fragments arranged by size:
2558 2320 2007 419 316 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2426 10
Size: 54 1425 343 594 5388
Fragments arranged by size:
5388 1425 594 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 2553 4541 5762 6121 6280 518
Size: 648 1218 169 1988 1221 359 159 2042
Fragments arranged by size:
2042 1988 1221 1218 648 359 169 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2873 3961 4582 4660 5220 5897 7045
Size: 642 625 1088 621 78 560 677 1148
Cuts at: 7045 7290 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1088 677 642 625 621 560
245 78
NcoI C'CATG_G
Cuts at: 513 1065 2667 3839 513
Size: 552 1602 1172 4478
Fragments arranged by size:
4478 1602 1172 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7804
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7804
NotI GC'GGCC_GC
Cuts at: 5251 5251
Size: 7804
NsiI A_TGCA'T
Cuts at: 2028 2028
Size: 7804
NspI r_CATG'y
Cuts at: 1132 1229 2152 2383 5162 6707 1132
Size: 97 923 231 2779 1545 2229
Fragments arranged by size:
2779 2229 1545 923 231 97
PasI CC'CwG_GG
Cuts at: 1348 2902 3440 1348
Size: 1554 538 5712
Fragments arranged by size:
5712 1554 538
PciI A'CATG_T
Cuts at: 2148 6703 2148
Size: 4555 3249
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2764 3875 4696 1108
Size: 1656 1111 821 4216
Fragments arranged by size:
4216 1656 1111 821
PfoI T'CCnGG_A
Cuts at: 3417 3417
Size: 7804
PmeI GTTT'AAAC
Cuts at: 5108 5108
Size: 7804
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5873 5905 7386 7418 5873
Size: 32 1481 32 6259
Fragments arranged by size:
6259 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3829 4009 4090 4588 3829
Size: 180 81 498 7045
Fragments arranged by size:
7045 498 180 81
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7804
PsiI TTA'TAA
Cuts at: 2199 5374 2199
Size: 3175 4629
PspXI vC'TCGA_Gb
Cuts at: 2877 2877
Size: 7804
PstI C_TGCA'G
Cuts at: 830 2253 2394 2899 3145 3960 3969 4641 5156
Size: 1423 141 505 246 815 9 672 515
Cuts at: 5156 830
Size: 3478
Fragments arranged by size:
3478 1423 815 672 515 505 246 141
9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7804
PvuII CAG'CTG
Cuts at: 2248 2873 3961 4582 4660 2248
Size: 625 1088 621 78 5392
Fragments arranged by size:
5392 1088 625 621 78
SalI G'TCGA_C
Cuts at: 1936 5146 1936
Size: 3210 4594
SapI GCTCTTCn'nnn_
Cuts at: 3212 4623 4883 3212
Size: 1411 260 6133
Fragments arranged by size:
6133 1411 260
SbfI CC_TGCA'GG
Cuts at: 5156 5156
Size: 7804
ScaI AGT'ACT
Cuts at: 2857 6052 7764 2857
Size: 3195 1712 2897
Fragments arranged by size:
3195 2897 1712
SfcI C'TryA_G
Cuts at: 826 1045 2249 2390 2433 2895 3141 3956 3965
Size: 219 1204 141 43 462 246 815 9
Cuts at: 3965 4637 5152 6287 6968 7159 826
Size: 672 515 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 815 681 672 515 462
246 219 191 141 43 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7804
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7804
SmaI CCC'GGG
Cuts at: 1872 5131 1872
Size: 3259 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2492 2877 3296 3807 4013 4181
Size: 197 926 549 385 419 511 206 168
Cuts at: 4181 5908 6809 7071 820
Size: 1727 901 262 1553
Fragments arranged by size:
1727 1553 926 901 549 511 419 385
262 206 197 168
SnaBI TAC'GTA
Cuts at: 493 5101 5595 493
Size: 4608 494 2702
Fragments arranged by size:
4608 2702 494
SpeI A'CTAG_T
Cuts at: 152 3172 152
Size: 3020 4784
SphI G_CATG'C
Cuts at: 1132 5162 1132
Size: 4030 3774
SspI AAT'ATT
Cuts at: 5 52 5728 5
Size: 47 5676 2081
Fragments arranged by size:
5676 2081 47
StuI AGG'CCT
Cuts at: 1517 3584 4126 1517
Size: 2067 542 5195
Fragments arranged by size:
5195 2067 542
StyI C'CwwG_G
Cuts at: 513 1065 2667 3210 3839 5243 513
Size: 552 1602 543 629 1404 3074
Fragments arranged by size:
3074 1602 1404 629 552 543
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2756 2771 3734 3933 4139 5831 5848
Size: 1255 1176 15 963 199 206 1692 17
Cuts at: 5848 6001 6186 6631 325
Size: 153 185 445 1498
Fragments arranged by size:
1692 1498 1255 1176 963 445 206 199
185 153 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2502 2855 3543
Size: 274 80 33 51 1443 525 353 688
Cuts at: 3543 3972 6050 7762 96
Size: 429 2078 1712 138
Fragments arranged by size:
2078 1712 1443 688 525 429 353 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 4458 4490 7764 7796 2138
Size: 32 2288 32 3274 32 2146
Fragments arranged by size:
3274 2288 2146 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3847 1316
Size: 2531 5273
XbaI T'CTAG_A
Cuts at: 2794 5140 2794
Size: 2346 5458
XhoI C'TCGA_G
Cuts at: 1943 2877 3807 1943
Size: 934 930 5940
Fragments arranged by size:
5940 934 930
XmaI C'CCGG_G
Cuts at: 1870 5129 1870
Size: 3259 4545
XmnI GAAnn'nnTTC
Cuts at: 4331 5933 4331
Size: 1602 6202
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5612 276
Size: 53 83 186 936 4078 2468
Fragments arranged by size:
4078 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI
BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI