Restriction map for
pFN21ASDA1701
(Circular) (Six-base) MAPSORT of: pFN21ASDA1701.seq Check: 1539 from: 1 to: 6370
pFN21ASDA1701 6370 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2914 4180 278
Size: 53 83 186 936 1378 1266 2468
Fragments arranged by size:
2468 1378 1266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3713 1937
Size: 1776 4594
Acc65I G'GTAC_C
Cuts at: 3691 3691
Size: 6370
AclI AA'CG_TT
Cuts at: 1563 4497 4870 1563
Size: 2934 373 3063
Fragments arranged by size:
3063 2934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2799 4433 5817 6085 1632
Size: 1167 1634 1384 268 1917
Fragments arranged by size:
1917 1634 1384 1167 268
AflII C'TTAA_G
Cuts at: 820 1017 3017 820
Size: 197 2000 4173
Fragments arranged by size:
4173 2000 197
AflIII A'CryG_T
Cuts at: 1203 2321 3232 5269 6246 1203
Size: 1118 911 2037 977 1327
Fragments arranged by size:
2037 1327 1118 977 911
AgeI A'CCGG_T
Cuts at: 5201 5201
Size: 6370
AhdI GACnn_n'nnGTC
Cuts at: 5099 5099
Size: 6370
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2830 1184
Size: 1646 4724
AlwNI CAG_nnn'CTG
Cuts at: 2516 4113 5685 2516
Size: 1597 1572 3201
Fragments arranged by size:
3201 1597 1572
ApaLI G'TGCA_C
Cuts at: 4427 5583 4427
Size: 1156 5214
ApoI r'AATT_y
Cuts at: 1493 2993 3679 3905 4057 6166 1493
Size: 1500 686 226 152 2109 1697
Fragments arranged by size:
2109 1697 1500 686 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2637 4924 160
Size: 872 1605 2287 1606
Fragments arranged by size:
2287 1606 1605 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 3695 1870
Size: 73 1752 4545
Fragments arranged by size:
4545 1752 73
AvrII C'CTAG_G
Cuts at: 2504 2504
Size: 6370
BamHI G'GATC_C
Cuts at: 3700 4067 3700
Size: 367 6003
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2450 2731 3691 5146 618
Size: 325 217 627 663 281 960 1455 1842
Fragments arranged by size:
1842 1455 960 663 627 325 281 217
BanII G_rGCy'C
Cuts at: 1391 2053 2245 3689 1391
Size: 662 192 1444 4072
Fragments arranged by size:
4072 1444 662 192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5399
BbvCI CC'TCA_GC
Cuts at: 2466 2517 2619 2466
Size: 51 102 6217
Fragments arranged by size:
6217 102 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4561 4595 1903
Size: 34 2624 34 3678
Fragments arranged by size:
3678 2624 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4262 5472 4262
Size: 1210 5160
BclI T'GATC_A
Cuts at: 1548 3465 3598 1548
Size: 1917 133 4320
Fragments arranged by size:
4320 1917 133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4981 136
Size: 107 122 71 631 605 3309 1525
Fragments arranged by size:
3309 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6365 6365
Size: 6370
BlpI GC'TnA_GC
Cuts at: 3787 3787
Size: 6370
Bme1580I G_kGCm'C
Cuts at: 1790 2455 4431 5587 1790
Size: 665 1976 1156 2573
Fragments arranged by size:
2573 1976 1156 665
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6370
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4101 5059 442
Size: 416 53 83 399 24 2684 958 1753
Fragments arranged by size:
2684 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 2688 5030 1239
Size: 876 573 2342 2579
Fragments arranged by size:
2579 2342 876 573
Bpu10I CC'TnA_GC
Cuts at: 2466 2517 2619 2466
Size: 51 102 6217
Fragments arranged by size:
6217 102 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 4495 5360 5658 3625
Size: 870 865 298 4337
Fragments arranged by size:
4337 870 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5033 882
Size: 617 3534 2219
Fragments arranged by size:
3534 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3667 4161 493
Size: 711 2463 494 2702
Fragments arranged by size:
2702 2463 711 494
BsaBI GATnn'nnATC
Cuts at: 2722 6364 2722
Size: 3642 2728
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2911 4177 4559 5194
Size: 53 83 186 936 1378 1266 382 635
Cuts at: 5194 275
Size: 1451
Fragments arranged by size:
1451 1378 1266 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4803 5201 5475 5622 6033 4803
Size: 398 274 147 411 5140
Fragments arranged by size:
5140 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6145
Fragments arranged by size:
6145 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 2534 2537 2540 1186
Size: 1297 51 3 3 5016
Fragments arranged by size:
5016 1297 51 3 3
BseYI C'CCAG_C
Cuts at: 2027 2297 2360 2486 5573 6069 2027
Size: 270 63 126 3087 496 2328
Fragments arranged by size:
3087 2328 496 270 126 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5797
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3820 4581 5609 1540
Size: 275 186 1819 761 1028 2301
Fragments arranged by size:
2301 1819 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2245 4431 4516 5587 2245
Size: 2186 85 1071 3028
Fragments arranged by size:
3028 2186 1071 85
BsmI GAATG_Cn'
Cuts at: 2978 3881 3974 2978
Size: 903 93 5374
Fragments arranged by size:
5374 903 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2222 2222
Size: 6370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2245 2455 3689 4431 4516 5587
Size: 399 263 192 210 1234 742 85 1071
Cuts at: 5587 1391
Size: 2174
Fragments arranged by size:
2174 1234 1071 742 399 263 210 192
85
BspEI T'CCGG_A
Cuts at: 6033 6033
Size: 6370
BspHI T'CATG_A
Cuts at: 4259 5989 4259
Size: 1730 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 3725 844
Size: 1644 1237 3489
Fragments arranged by size:
3489 1644 1237
BsrBI CCG'CTC
Cuts at: 1394 1823 4257 1394
Size: 429 2434 3507
Fragments arranged by size:
3507 2434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3069 4865 5039 65
Size: 1183 1821 1796 174 1396
Fragments arranged by size:
1821 1796 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5014 5201 1700
Size: 110 3204 187 2869
Fragments arranged by size:
3204 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6370
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6370
BssSI C'ACGA_G
Cuts at: 4430 5442 4430
Size: 1012 5358
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3059 794
Size: 2265 4105
BstBI TT'CG_AA
Cuts at: 2007 5174 2007
Size: 3167 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6370
BstYI r'GATC_y
Cuts at: 1972 2723 3700 4067 4452 4469 5921 6007 6019
Size: 751 977 367 385 17 1452 86 12
Cuts at: 6019 6365 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 977 751 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3385 3385
Size: 6370
BtgI C'CryG_G
Cuts at: 513 1065 2022 2274 513
Size: 552 957 252 4609
Fragments arranged by size:
4609 957 552 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5631
Fragments arranged by size:
5631 683 56
BtsI GCAGTG_nn'
Cuts at: 2208 3886 4680 4700 2208
Size: 1678 794 20 3878
Fragments arranged by size:
3878 1678 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6335
DraI TTT'AAA
Cuts at: 3429 3674 3429
Size: 245 6125
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5377 809
Size: 507 4061 1802
Fragments arranged by size:
4061 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3543 3624 3817
Size: 54 1425 50 275 18 1713 81 193
Cuts at: 3817 4706 6335 8
Size: 889 1629 43
Fragments arranged by size:
1713 1629 1425 889 275 193 81 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3817 1537
Size: 275 2005 4090
Fragments arranged by size:
4090 2005 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2361 2397 2526 4300 1353
Size: 1008 36 129 1774 3423
Fragments arranged by size:
3423 1774 1008 129 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4957 5331 5477 387
Size: 1064 138 3368 374 146 1280
Fragments arranged by size:
3368 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2243 2243
Size: 6370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2688 2799 4433 5030 5817 6085
Size: 393 483 573 111 1634 597 787 268
Cuts at: 6085 1239
Size: 1524
Fragments arranged by size:
1634 1524 787 597 573 483 393 268
111
EcoNI CCTnn'n_nnAGG
Cuts at: 2508 2508
Size: 6370
EcoRI G'AATT_C
Cuts at: 3679 4057 3679
Size: 378 5992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2769 3545 2769
Size: 776 5594
FspI TGC'GCA
Cuts at: 4876 4876
Size: 6370
HaeII r_GCGC'y
Cuts at: 1678 5517 1678
Size: 3839 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2896 2901 2928 2933 3450
Size: 32 135 32 1324 5 27 5 517
Cuts at: 3450 3482 5011 5043 5085 5117 1373
Size: 32 1529 32 42 32 2626
Fragments arranged by size:
2626 1529 1324 517 135 42 32 32
32 32 32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3714 3960 669
Size: 988 281 1086 690 246 3079
Fragments arranged by size:
3079 1086 988 690 281 246
HindIII A'AGCT_T
Cuts at: 748 3136 748
Size: 2388 3982
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KpnI G_GTAC'C
Cuts at: 3695 3695
Size: 6370
MfeI C'AATT_G
Cuts at: 3585 3969 3585
Size: 384 5986
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2454 3470 5484 5668 1305
Size: 1149 1016 2014 184 2007
Fragments arranged by size:
2014 2007 1149 1016 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3545 3626 10
Size: 54 1425 343 1713 81 2754
Fragments arranged by size:
2754 1713 1425 343 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4328 4687 4846 518
Size: 648 3162 359 159 2042
Fragments arranged by size:
3162 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2708 2777 3786 4463 5611 5856 1606
Size: 1102 69 1009 677 1148 245 2120
Fragments arranged by size:
2120 1148 1102 1009 677 245 69
NcoI C'CATG_G
Cuts at: 513 1065 2022 2274 513
Size: 552 957 252 4609
Fragments arranged by size:
4609 957 552 252
NdeI CA'TA_TG
Cuts at: 387 3243 387
Size: 2856 3514
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6370
NotI GC'GGCC_GC
Cuts at: 3817 3817
Size: 6370
NspI r_CATG'y
Cuts at: 1132 1229 2325 3236 3728 5273 1132
Size: 97 1096 911 492 1545 2229
Fragments arranged by size:
2229 1545 1096 911 492 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6370
PciI A'CATG_T
Cuts at: 2321 3232 5269 2321
Size: 911 2037 3422
Fragments arranged by size:
3422 2037 911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2661 1108
Size: 1553 4817
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2854 4439 4471 5952 5984 2822
Size: 32 1585 32 1481 32 3208
Fragments arranged by size:
3208 1585 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6370
PsiI TTA'TAA
Cuts at: 3612 3940 3612
Size: 328 6042
PstI C_TGCA'G
Cuts at: 830 3454 3722 830
Size: 2624 268 3478
Fragments arranged by size:
3478 2624 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
PvuII CAG'CTG
Cuts at: 2708 2708
Size: 6370
SacI G_AGCT'C
Cuts at: 2245 2245
Size: 6370
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SbfI CC_TGCA'GG
Cuts at: 3722 3722
Size: 6370
ScaI AGT'ACT
Cuts at: 4618 6330 4618
Size: 1712 4658
SfcI C'TryA_G
Cuts at: 826 1045 2130 3450 3718 4853 5534 5725 826
Size: 219 1085 1320 268 1135 681 191 1471
Fragments arranged by size:
1471 1320 1135 1085 681 268 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6370
SmaI CCC'GGG
Cuts at: 1872 3697 1872
Size: 1825 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3017 3604 4474 5375 5637 820
Size: 197 926 1074 587 870 901 262 1553
Fragments arranged by size:
1553 1074 926 901 870 587 262 197
SnaBI TAC'GTA
Cuts at: 493 3667 4161 493
Size: 3174 494 2702
Fragments arranged by size:
3174 2702 494
SpeI A'CTAG_T
Cuts at: 152 2573 152
Size: 2421 3949
SphI G_CATG'C
Cuts at: 1132 3728 1132
Size: 2596 3774
SspI AAT'ATT
Cuts at: 5 52 4294 5
Size: 47 4242 2081
Fragments arranged by size:
4242 2081 47
StuI AGG'CCT
Cuts at: 1517 2128 1517
Size: 611 5759
StyI C'CwwG_G
Cuts at: 513 1065 2022 2274 2292 2504 2655 3809 513
Size: 552 957 252 18 212 151 1154 3074
Fragments arranged by size:
3074 1154 957 552 252 212 151 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3056 3099 4397 4414 4567 4752 5197
Size: 1255 1476 43 1298 17 153 185 445
Cuts at: 5197 325
Size: 1498
Fragments arranged by size:
1498 1476 1298 1255 445 185 153 43
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4616 6328 96
Size: 274 80 33 51 1443 2639 1712 138
Fragments arranged by size:
2639 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2838 2870 6330 6362 2838
Size: 32 3460 32 2846
Fragments arranged by size:
3460 2846 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2167 1316
Size: 851 5519
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6370
XmaI C'CCGG_G
Cuts at: 1870 3695 1870
Size: 1825 4545
XmnI GAAnn'nnTTC
Cuts at: 4499 4499
Size: 6370
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2912 4178 276
Size: 53 83 186 936 1378 1266 2468
Fragments arranged by size:
2468 1378 1266 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI