Restriction map for
pFN21ASDA1696
(Circular) (Six-base) MAPSORT of: pFN21ASDA1696.seq Check: 4116 from: 1 to: 6631
pFN21ASDA1696 6631 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3088 3088
Size: 6631
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2570 4441 278
Size: 53 83 186 936 1034 1871 2468
Fragments arranged by size:
2468 1871 1034 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3222 3786 3974 1937
Size: 1285 564 188 4594
Fragments arranged by size:
4594 1285 564 188
Acc65I G'GTAC_C
Cuts at: 3952 3952
Size: 6631
AclI AA'CG_TT
Cuts at: 1563 2915 4758 5131 1563
Size: 1352 1843 373 3063
Fragments arranged by size:
3063 1843 1352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3515 4694 6078 6346 1632
Size: 1883 1179 1384 268 1917
Fragments arranged by size:
1917 1883 1384 1179 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6434
AflIII A'CryG_T
Cuts at: 1203 3398 5530 6507 1203
Size: 2195 2132 977 1327
Fragments arranged by size:
2195 2132 1327 977
AgeI A'CCGG_T
Cuts at: 5462 5462
Size: 6631
AhdI GACnn_n'nnGTC
Cuts at: 5360 5360
Size: 6631
AleI CACnn'nnGTG
Cuts at: 1166 2537 1166
Size: 1371 5260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2367 2555 1184
Size: 1183 188 5260
Fragments arranged by size:
5260 1183 188
AlwNI CAG_nnn'CTG
Cuts at: 2080 2447 2600 3385 3493 4374 5946 2080
Size: 367 153 785 108 881 1572 2765
Fragments arranged by size:
2765 1572 881 785 367 153 108
ApaLI G'TGCA_C
Cuts at: 4688 5844 4688
Size: 1156 5475
ApoI r'AATT_y
Cuts at: 1493 2061 2860 3940 4166 4318 6427 1493
Size: 568 799 1080 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 799 568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5185 160
Size: 872 4153 1606
Fragments arranged by size:
4153 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
AvaI C'yCGr_G
Cuts at: 1870 1943 3956 1870
Size: 73 2013 4545
Fragments arranged by size:
4545 2013 73
BamHI G'GATC_C
Cuts at: 2101 3961 4328 2101
Size: 1860 367 4404
Fragments arranged by size:
4404 1860 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3952 5407 618
Size: 325 217 627 2165 1455 1842
Fragments arranged by size:
2165 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3950 1391
Size: 2559 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2390 928
Size: 971 491 5169
Fragments arranged by size:
5169 971 491
BbvCI CC'TCA_GC
Cuts at: 2655 2796 2655
Size: 141 6490
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4822 4856 1903
Size: 34 2885 34 3678
Fragments arranged by size:
3678 2885 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4523 5733 4523
Size: 1210 5421
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6631
BfrBI ATG'CAT
Cuts at: 3675 3675
Size: 6631
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5242 136
Size: 107 122 71 631 605 3570 1525
Fragments arranged by size:
3570 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6626 6626
Size: 6631
BlpI GC'TnA_GC
Cuts at: 2528 2993 3653 4048 2528
Size: 465 660 395 5111
Fragments arranged by size:
5111 660 465 395
Bme1580I G_kGCm'C
Cuts at: 1790 4692 5848 1790
Size: 2902 1156 2573
Fragments arranged by size:
2902 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2705 1318
Size: 1387 5244
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2592 4362 5320
Size: 416 53 83 399 24 1175 1770 958
Cuts at: 5320 442
Size: 1753
Fragments arranged by size:
1770 1753 1175 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2546 2940 3749 5291 1239
Size: 1307 394 809 1542 2579
Fragments arranged by size:
2579 1542 1307 809 394
Bpu10I CC'TnA_GC
Cuts at: 2655 2796 2655
Size: 141 6490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4756 5621 5919 4756
Size: 865 298 5468
Fragments arranged by size:
5468 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2473 5294 882
Size: 617 974 2821 2219
Fragments arranged by size:
2821 2219 974 617
BsaAI yAC'GTr
Cuts at: 493 1204 3928 4422 493
Size: 711 2724 494 2702
Fragments arranged by size:
2724 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6625 6625
Size: 6631
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2567 4438 4820 5455
Size: 53 83 186 936 1034 1871 382 635
Cuts at: 5455 275
Size: 1451
Fragments arranged by size:
1871 1451 1034 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2227 3217 5064 5462 5736 5883 6294 2227
Size: 990 1847 398 274 147 411 2564
Fragments arranged by size:
2564 1847 990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6406
Fragments arranged by size:
6406 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2643 2784 3119 3868 1186
Size: 1457 141 335 749 3949
Fragments arranged by size:
3949 1457 749 335 141
BseYI C'CCAG_C
Cuts at: 2340 2767 2928 5834 6330 2340
Size: 427 161 2906 496 2641
Fragments arranged by size:
2906 2641 496 427 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2561 3069 3282 3814 3874 1163
Size: 573 825 508 213 532 60 3920
Fragments arranged by size:
3920 825 573 532 508 213 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4081 4842 5870 1540
Size: 275 186 2080 761 1028 2301
Fragments arranged by size:
2301 2080 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2529 4692 4777 5848 2529
Size: 2163 85 1071 3312
Fragments arranged by size:
3312 2163 1071 85
BsmI GAATG_Cn'
Cuts at: 2116 4142 4235 2116
Size: 2026 93 4512
Fragments arranged by size:
4512 2026 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2529 3950 4692 4777 5848 1391
Size: 399 739 1421 742 85 1071 2174
Fragments arranged by size:
2174 1421 1071 742 739 399 85
BspEI T'CCGG_A
Cuts at: 6294 6294
Size: 6631
BspHI T'CATG_A
Cuts at: 2134 3343 4520 6250 2134
Size: 1209 1177 1730 2515
Fragments arranged by size:
2515 1730 1209 1177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2626 3088 3986 844
Size: 1782 462 898 3489
Fragments arranged by size:
3489 1782 898 462
BsrBI CCG'CTC
Cuts at: 1394 1823 4518 1394
Size: 429 2695 3507
Fragments arranged by size:
3507 2695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2249 2487 5126 5300 65
Size: 1183 1001 238 2639 174 1396
Fragments arranged by size:
2639 1396 1183 1001 238 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3862 5275 5462 1700
Size: 110 2052 1413 187 2869
Fragments arranged by size:
2869 2052 1413 187 110
BsrGI T'GTAC_A
Cuts at: 96 2986 96
Size: 2890 3741
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6631
BssSI C'ACGA_G
Cuts at: 4691 5703 4691
Size: 1012 5619
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2447 3363 794
Size: 1653 916 4062
Fragments arranged by size:
4062 1653 916
BstBI TT'CG_AA
Cuts at: 2007 5435 2007
Size: 3428 3203
BstEII G'GTnAC_C
Cuts at: 1184 2497 1184
Size: 1313 5318
BstXI CCAn_nnnn'nTGG
Cuts at: 2494 2740 2494
Size: 246 6385
BstYI r'GATC_y
Cuts at: 1972 2101 3465 3961 4328 4713 4730 6182 6268
Size: 129 1364 496 367 385 17 1452 86
Cuts at: 6268 6280 6626 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1364 496 385 367 346 129
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2922 3515 3760 513
Size: 552 957 900 593 245 3384
Fragments arranged by size:
3384 957 900 593 552 245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5892
Fragments arranged by size:
5892 683 56
BtsI GCAGTG_nn'
Cuts at: 3270 4147 4941 4961 3270
Size: 877 794 20 4940
Fragments arranged by size:
4940 877 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6596
DraI TTT'AAA
Cuts at: 3612 3935 3612
Size: 323 6308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5638 809
Size: 507 4322 1802
Fragments arranged by size:
4322 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3326 3376 4078
Size: 54 1425 50 275 18 1496 50 702
Cuts at: 4078 4967 6596 8
Size: 889 1629 43
Fragments arranged by size:
1629 1496 1425 889 702 275 54 50
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4078 1537
Size: 275 2266 4090
Fragments arranged by size:
4090 2266 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3251 3299 3570 3909 4561 1353
Size: 1898 48 271 339 652 3423
Fragments arranged by size:
3423 1898 652 339 271 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5218 5592 5738 387
Size: 1064 138 3629 374 146 1280
Fragments arranged by size:
3629 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2546 2940 3515 3749 4694 5291 6078
Size: 393 914 394 575 234 945 597 787
Cuts at: 6078 6346 1239
Size: 268 1524
Fragments arranged by size:
1524 945 914 787 597 575 394 393
268 234
EcoNI CCTnn'n_nnAGG
Cuts at: 3731 3731
Size: 6631
EcoO109I rG'GnC_Cy
Cuts at: 3297 3297
Size: 6631
EcoRI G'AATT_C
Cuts at: 2860 3940 4318 2860
Size: 1080 378 5173
Fragments arranged by size:
5173 1080 378
FspI TGC'GCA
Cuts at: 5137 5137
Size: 6631
HaeII r_GCGC'y
Cuts at: 1678 5778 1678
Size: 4100 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2410 2442 2938 2970 5272
Size: 32 135 32 838 32 496 32 2302
Cuts at: 5272 5304 5346 5378 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2302 838 496 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2557 3975 4221 669
Size: 988 281 619 1418 246 3079
Fragments arranged by size:
3079 1418 988 619 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6631
HpaI GTT'AAC
Cuts at: 4221 4221
Size: 6631
KpnI G_GTAC'C
Cuts at: 3956 3956
Size: 6631
MfeI C'AATT_G
Cuts at: 3534 4230 3534
Size: 696 5935
MluI A'CGCG_T
Cuts at: 6507 6507
Size: 6631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5745 5929 1305
Size: 4440 184 2007
Fragments arranged by size:
4440 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3328 3378 10
Size: 54 1425 343 1496 50 3263
Fragments arranged by size:
3263 1496 1425 343 54 50
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2537 3680 4589 4948 5107 518
Size: 648 1326 45 1143 909 359 159 2042
Fragments arranged by size:
2042 1326 1143 909 648 359 159 45
MspA1I CmG'CkG
Cuts at: 1606 2351 3745 4047 4724 5872 6117 1606
Size: 745 1394 302 677 1148 245 2120
Fragments arranged by size:
2120 1394 1148 745 677 302 245
NaeI GCC'GGC
Cuts at: 3864 3864
Size: 6631
NcoI C'CATG_G
Cuts at: 513 1065 2022 2922 3515 513
Size: 552 957 900 593 3629
Fragments arranged by size:
3629 957 900 593 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6631
NgoMIV G'CCGG_C
Cuts at: 3862 3862
Size: 6631
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6631
NotI GC'GGCC_GC
Cuts at: 4078 4078
Size: 6631
NsiI A_TGCA'T
Cuts at: 3677 3677
Size: 6631
NspI r_CATG'y
Cuts at: 1132 1229 3402 3463 3989 5534 1132
Size: 97 2173 61 526 1545 2229
Fragments arranged by size:
2229 2173 1545 526 97 61
PasI CC'CwG_GG
Cuts at: 1348 3293 1348
Size: 1945 4686
PciI A'CATG_T
Cuts at: 3398 5530 3398
Size: 2132 4499
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 2611 3521 1108
Size: 972 531 910 4218
Fragments arranged by size:
4218 972 910 531
PmeI GTTT'AAAC
Cuts at: 3935 3935
Size: 6631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4700 4732 6213 6245 4700
Size: 32 1481 32 5086
Fragments arranged by size:
5086 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3297 3297
Size: 6631
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6631
PsiI TTA'TAA
Cuts at: 4201 4201
Size: 6631
PstI C_TGCA'G
Cuts at: 830 2356 2637 3983 830
Size: 1526 281 1346 3478
Fragments arranged by size:
3478 1526 1346 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6631
PvuII CAG'CTG
Cuts at: 2351 3745 2351
Size: 1394 5237
SalI G'TCGA_C
Cuts at: 1936 3973 1936
Size: 2037 4594
SanDI GG'GwC_CC
Cuts at: 3297 3297
Size: 6631
SbfI CC_TGCA'GG
Cuts at: 3983 3983
Size: 6631
ScaI AGT'ACT
Cuts at: 4879 6591 4879
Size: 1712 4919
SfcI C'TryA_G
Cuts at: 826 1045 2039 2297 2352 2394 2633 3223 3783
Size: 219 994 258 55 42 239 590 560
Cuts at: 3783 3979 5114 5795 5986 826
Size: 196 1135 681 191 1471
Fragments arranged by size:
1471 1135 994 681 590 560 258 239
219 196 191 55 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6631
SmaI CCC'GGG
Cuts at: 1872 3958 1872
Size: 2086 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4735 5636 5898 820
Size: 197 926 2792 901 262 1553
Fragments arranged by size:
2792 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3928 4422 493
Size: 3435 494 2702
Fragments arranged by size:
3435 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6631
SphI G_CATG'C
Cuts at: 1132 3989 1132
Size: 2857 3774
SspI AAT'ATT
Cuts at: 5 52 4555 5
Size: 47 4503 2081
Fragments arranged by size:
4503 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6631
StyI C'CwwG_G
Cuts at: 513 1065 2022 2922 3453 3515 4070 513
Size: 552 957 900 531 62 555 3074
Fragments arranged by size:
3074 957 900 555 552 531 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3103 4658 4675 4828 5013 5458 325
Size: 1255 1523 1555 17 153 185 445 1498
Fragments arranged by size:
1555 1523 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2986 4877 6589
Size: 274 80 33 51 1443 1009 1891 1712
Cuts at: 6589 96
Size: 138
Fragments arranged by size:
1891 1712 1443 1009 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2664 2689 2696 2721 2830 2862 6591 6623 2664
Size: 25 7 25 109 32 3729 32 2672
Fragments arranged by size:
3729 2672 109 32 32 25 25 7
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6631
XbaI T'CTAG_A
Cuts at: 3163 3967 3163
Size: 804 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6631
XmaI C'CCGG_G
Cuts at: 1870 3956 1870
Size: 2086 4545
XmnI GAAnn'nnTTC
Cuts at: 3510 4760 3510
Size: 1250 5381
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2568 4439 276
Size: 53 83 186 936 1034 1871 2468
Fragments arranged by size:
2468 1871 1034 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI