Restriction map for
pFN21ASDA1691
(Circular) (Six-base) MAPSORT of: pFN21ASDA1691.seq Check: 6545 from: 1 to: 6298
pFN21ASDA1691 6298 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2614 2614
Size: 6298
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2685 4108 278
Size: 53 83 186 936 1149 1423 2468
Fragments arranged by size:
2468 1423 1149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3641 1937
Size: 1704 4594
Acc65I G'GTAC_C
Cuts at: 3619 3619
Size: 6298
AclI AA'CG_TT
Cuts at: 1563 4425 4798 1563
Size: 2862 373 3063
Fragments arranged by size:
3063 2862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4361 5745 6013 1632
Size: 2729 1384 268 1917
Fragments arranged by size:
2729 1917 1384 268
AfeI AGC'GCT
Cuts at: 3150 3150
Size: 6298
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6101
AflIII A'CryG_T
Cuts at: 1203 5197 6174 1203
Size: 3994 977 1327
Fragments arranged by size:
3994 1327 977
AgeI A'CCGG_T
Cuts at: 5129 5129
Size: 6298
AhdI GACnn_n'nnGTC
Cuts at: 5027 5027
Size: 6298
AleI CACnn'nnGTG
Cuts at: 1166 2306 2441 2597 3083 1166
Size: 1140 135 156 486 4381
Fragments arranged by size:
4381 1140 486 156 135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6298
AlwNI CAG_nnn'CTG
Cuts at: 2149 2482 2533 2542 2953 4041 5613 2149
Size: 333 51 9 411 1088 1572 2834
Fragments arranged by size:
2834 1572 1088 411 333 51 9
ApaLI G'TGCA_C
Cuts at: 4355 5511 4355
Size: 1156 5142
ApoI r'AATT_y
Cuts at: 1493 3607 3833 3985 6094 1493
Size: 2114 226 152 2109 1697
Fragments arranged by size:
2114 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4852 160
Size: 872 3820 1606
Fragments arranged by size:
3820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 2507 3623 1870
Size: 73 180 384 1116 4545
Fragments arranged by size:
4545 1116 384 180 73
BamHI G'GATC_C
Cuts at: 3628 3995 3628
Size: 367 5931
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 3619 5074 618
Size: 325 217 627 257 1575 1455 1842
Fragments arranged by size:
1842 1575 1455 627 325 257 217
BanII G_rGCy'C
Cuts at: 1391 2469 2514 2822 3617 1391
Size: 1078 45 308 795 4072
Fragments arranged by size:
4072 1078 795 308 45
BbeI G_GCGC'C
Cuts at: 2048 2048
Size: 6298
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4489 4523 1903
Size: 34 2552 34 3678
Fragments arranged by size:
3678 2552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4190 5400 4190
Size: 1210 5088
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6298
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 4909 136
Size: 107 122 71 631 605 502 2735 1525
Fragments arranged by size:
2735 1525 631 605 502 122 107 71
BglII A'GATC_T
Cuts at: 6293 6293
Size: 6298
BlpI GC'TnA_GC
Cuts at: 3000 3715 3000
Size: 715 5583
Bme1580I G_kGCm'C
Cuts at: 1790 4359 5515 1790
Size: 2569 1156 2573
Fragments arranged by size:
2573 2569 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4029 4987 442
Size: 416 53 83 399 24 2612 958 1753
Fragments arranged by size:
2612 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2893 3582 2893
Size: 689 5609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 2567 2627 2806 3291 3514 3562 4958
Size: 1265 63 60 179 485 223 48 1396
Cuts at: 4958 1239
Size: 2579
Fragments arranged by size:
2579 1396 1265 485 223 179 63 60
48
Bpu10I CC'TnA_GC
Cuts at: 2090 2090
Size: 6298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4423 5288 5586 4423
Size: 865 298 5135
Fragments arranged by size:
5135 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2345 3383 4961 882
Size: 617 846 1038 1578 2219
Fragments arranged by size:
2219 1578 1038 846 617
BsaAI yAC'GTr
Cuts at: 493 1204 2854 3142 3595 4089 493
Size: 711 1650 288 453 494 2702
Fragments arranged by size:
2702 1650 711 494 453 288
BsaBI GATnn'nnATC
Cuts at: 6292 6292
Size: 6298
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 2262 2682 2847
Size: 53 83 186 936 512 217 420 165
Cuts at: 2847 4105 4487 5122 275
Size: 1258 382 635 1451
Fragments arranged by size:
1451 1258 936 635 512 420 382 217
186 165 83 53
BsaWI w'CCGG_w
Cuts at: 4731 5129 5403 5550 5961 4731
Size: 398 274 147 411 5068
Fragments arranged by size:
5068 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6073
Fragments arranged by size:
6073 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2264 2885 3041 3308 3311 1186
Size: 1000 78 621 156 267 3 4173
Fragments arranged by size:
4173 1000 621 267 156 78 3
BseYI C'CCAG_C
Cuts at: 2094 2468 2557 2653 2755 2781 2791 3353 5501
Size: 374 89 96 102 26 10 562 2148
Cuts at: 5501 5997 2094
Size: 496 2395
Fragments arranged by size:
2395 2148 562 496 374 102 96 89
26 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 2260 2332 1163
Size: 573 315 209 72 5129
Fragments arranged by size:
5129 573 315 209 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2571 3405 3748 4509 5537 1540
Size: 275 186 570 834 343 761 1028 2301
Fragments arranged by size:
2301 1028 834 761 570 343 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 4359 4444 5515 2256
Size: 2103 85 1071 3039
Fragments arranged by size:
3039 2103 1071 85
BsmI GAATG_Cn'
Cuts at: 3809 3902 3809
Size: 93 6205
BsmBI CGTCTCn'nnnn_
Cuts at: 2543 2543
Size: 6298
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2469 2514 2822 3617 4359 4444
Size: 399 466 213 45 308 795 742 85
Cuts at: 4444 5515 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 795 742 466 399 308 213
85 45
BspEI T'CCGG_A
Cuts at: 5961 5961
Size: 6298
BspHI T'CATG_A
Cuts at: 4187 5917 4187
Size: 1730 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2614 3099 3653 844
Size: 1770 485 554 3489
Fragments arranged by size:
3489 1770 554 485
BsrBI CCG'CTC
Cuts at: 1394 1823 2488 3305 4185 1394
Size: 429 665 817 880 3507
Fragments arranged by size:
3507 880 817 665 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4793 4967 65
Size: 1183 3545 174 1396
Fragments arranged by size:
3545 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2070 2232 2318 3031 3155 4942 5129
Size: 110 260 162 86 713 124 1787 187
Cuts at: 5129 1700
Size: 2869
Fragments arranged by size:
2869 1787 713 260 187 162 124 110
86
BsrGI T'GTAC_A
Cuts at: 96 3360 96
Size: 3264 3034
BssHII G'CGCG_C
Cuts at: 1925 2787 1925
Size: 862 5436
BssSI C'ACGA_G
Cuts at: 3556 4358 5370 3556
Size: 802 1012 4484
Fragments arranged by size:
4484 1012 802
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2074 2149 2542 794
Size: 1280 75 393 4550
Fragments arranged by size:
4550 1280 393 75
BstBI TT'CG_AA
Cuts at: 2007 5102 2007
Size: 3095 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6298
BstXI CCAn_nnnn'nTGG
Cuts at: 2866 2866
Size: 6298
BstYI r'GATC_y
Cuts at: 1972 2629 3628 3995 4380 4397 5849 5935 5947
Size: 657 999 367 385 17 1452 86 12
Cuts at: 5947 6293 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 999 657 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2814 3314 3405 513
Size: 552 957 792 500 91 3406
Fragments arranged by size:
3406 957 792 552 500 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2281 2308 487
Size: 683 56 1055 27 4477
Fragments arranged by size:
4477 1055 683 56 27
BtsI GCAGTG_nn'
Cuts at: 2334 3391 3814 4608 4628 2334
Size: 1057 423 794 20 4004
Fragments arranged by size:
4004 1057 794 423 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6263
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5305 809
Size: 507 3989 1802
Fragments arranged by size:
3989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2409 2496
Size: 54 1425 50 275 18 195 384 87
Cuts at: 2496 2568 3033 3402 3745 4634 6263 8
Size: 72 465 369 343 889 1629 43
Fragments arranged by size:
1629 1425 889 465 384 369 343 275
195 87 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2568 3402 3745 1537
Size: 275 756 834 343 4090
Fragments arranged by size:
4090 834 756 343 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2204 2213 3206 4228 1353
Size: 851 9 993 1022 3423
Fragments arranged by size:
3423 1022 993 851 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2378 2477 2633 4885 5259 5405
Size: 1064 138 789 99 156 2252 374 146
Cuts at: 5405 387
Size: 1280
Fragments arranged by size:
2252 1280 1064 789 374 156 146 138
99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2504 2567 2627 2806 3291 3514 3562
Size: 393 872 63 60 179 485 223 48
Cuts at: 3562 4361 4958 5745 6013 1239
Size: 799 597 787 268 1524
Fragments arranged by size:
1524 872 799 787 597 485 393 268
223 179 63 60 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2584 2584
Size: 6298
EcoO109I rG'GnC_Cy
Cuts at: 2146 2539 3043 3181 3275 2146
Size: 393 504 138 94 5169
Fragments arranged by size:
5169 504 393 138 94
EcoRI G'AATT_C
Cuts at: 3607 3985 3607
Size: 378 5920
FspI TGC'GCA
Cuts at: 4804 4804
Size: 6298
HaeII r_GCGC'y
Cuts at: 1678 2048 2319 3152 5445 1678
Size: 370 271 833 2293 2531
Fragments arranged by size:
2531 2293 833 370 271
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4939 4971 5013 5045 1373
Size: 32 135 32 3367 32 42 32 2626
Fragments arranged by size:
3367 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3642 3888 669
Size: 988 281 1704 246 3079
Fragments arranged by size:
3079 1704 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6298
HpaI GTT'AAC
Cuts at: 3888 3888
Size: 6298
KasI G'GCGC_C
Cuts at: 2044 2044
Size: 6298
KpnI G_GTAC'C
Cuts at: 3623 3623
Size: 6298
MfeI C'AATT_G
Cuts at: 3897 3897
Size: 6298
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5412 5596 1305
Size: 4107 184 2007
Fragments arranged by size:
4107 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4476
Fragments arranged by size:
4476 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 2327 2441 2597 2738 3083 4256
Size: 648 1140 21 114 156 141 345 1173
Cuts at: 4256 4615 4774 518
Size: 359 159 2042
Fragments arranged by size:
2042 1173 1140 648 359 345 159 156
141 114 21
MspA1I CmG'CkG
Cuts at: 1606 2494 2781 3316 3366 3407 3477 3582 3714
Size: 888 287 535 50 41 70 105 132
Cuts at: 3714 4391 5539 5784 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 677 535 287 245 132
105 70 50 41
NaeI GCC'GGC
Cuts at: 2234 2320 3033 2234
Size: 86 713 5499
Fragments arranged by size:
5499 713 86
NarI GG'CG_CC
Cuts at: 2045 2045
Size: 6298
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4789
Fragments arranged by size:
4789 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6298
NgoMIV G'CCGG_C
Cuts at: 2232 2318 3031 2232
Size: 86 713 5499
Fragments arranged by size:
5499 713 86
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6298
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NspI r_CATG'y
Cuts at: 1132 1229 3352 3656 5201 1132
Size: 97 2123 304 1545 2229
Fragments arranged by size:
2229 2123 1545 304 97
PasI CC'CwG_GG
Cuts at: 1348 3021 1348
Size: 1673 4625
PciI A'CATG_T
Cuts at: 5197 5197
Size: 6298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3497 1108
Size: 2389 3909
PfoI T'CCnGG_A
Cuts at: 2418 3060 2418
Size: 642 5656
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3043 3043
Size: 6298
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6298
PsiI TTA'TAA
Cuts at: 3868 3868
Size: 6298
PstI C_TGCA'G
Cuts at: 830 2478 2538 2904 3096 3245 3650 830
Size: 1648 60 366 192 149 405 3478
Fragments arranged by size:
3478 1648 405 366 192 149 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
PvuII CAG'CTG
Cuts at: 2494 2781 3366 2494
Size: 287 585 5426
Fragments arranged by size:
5426 585 287
SacII CC_GC'GG
Cuts at: 3317 3408 3317
Size: 91 6207
SalI G'TCGA_C
Cuts at: 1936 3640 1936
Size: 1704 4594
SapI GCTCTTCn'nnn_
Cuts at: 2213 2213
Size: 6298
SbfI CC_TGCA'GG
Cuts at: 3650 3650
Size: 6298
ScaI AGT'ACT
Cuts at: 2876 2906 4546 6258 2876
Size: 30 1640 1712 2916
Fragments arranged by size:
2916 1712 1640 30
SfcI C'TryA_G
Cuts at: 826 1045 2474 2534 2900 3092 3241 3646 4781
Size: 219 1429 60 366 192 149 405 1135
Cuts at: 4781 5462 5653 826
Size: 681 191 1471
Fragments arranged by size:
1471 1429 1135 681 405 366 219 192
191 149 60
SfoI GGC'GCC
Cuts at: 2046 2046
Size: 6298
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6298
SmaI CCC'GGG
Cuts at: 1872 3625 1872
Size: 1753 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4402 5303 5565 820
Size: 197 926 2459 901 262 1553
Fragments arranged by size:
2459 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3595 4089 493
Size: 3102 494 2702
Fragments arranged by size:
3102 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6298
SphI G_CATG'C
Cuts at: 1132 3656 1132
Size: 2524 3774
SspI AAT'ATT
Cuts at: 5 52 4222 5
Size: 47 4170 2081
Fragments arranged by size:
4170 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6298
StyI C'CwwG_G
Cuts at: 513 1065 2022 2727 3737 513
Size: 552 957 705 1010 3074
Fragments arranged by size:
3074 1010 957 705 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2044 4325 4342 4495 4680 5125 325
Size: 1255 464 2281 17 153 185 445 1498
Fragments arranged by size:
2281 1498 1255 464 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2874 2904 3360
Size: 274 80 33 51 1443 897 30 456
Cuts at: 3360 3441 4544 6256 96
Size: 81 1103 1712 138
Fragments arranged by size:
1712 1443 1103 897 456 274 138 81
80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 6258 6290 2414
Size: 32 3812 32 2422
Fragments arranged by size:
3812 2422 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6298
XbaI T'CTAG_A
Cuts at: 3634 3634
Size: 6298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2404 2734 2404
Size: 330 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 3623 1870
Size: 1753 4545
XmnI GAAnn'nnTTC
Cuts at: 4427 4427
Size: 6298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2683 4106 276
Size: 53 83 186 936 1149 1423 2468
Fragments arranged by size:
2468 1423 1149 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SanDI SexAI SfiI SrfI SwaI