Restriction map for
pFN21ASDA1688
(Circular) (Six-base) MAPSORT of: pFN21ASDA1688.seq Check: 3968 from: 1 to: 7978
pFN21ASDA1688 7978 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4849 4849
Size: 7978
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5788 278
Size: 53 83 186 936 4252 2468
Fragments arranged by size:
4252 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2064 3219 5321 1937
Size: 127 1155 2102 4594
Fragments arranged by size:
4594 2102 1155 127
Acc65I G'GTAC_C
Cuts at: 4929 5299 4929
Size: 370 7608
AclI AA'CG_TT
Cuts at: 1563 6105 6478 1563
Size: 4542 373 3063
Fragments arranged by size:
4542 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2369 2870 3814 4643 4798 4871 4940 6041
Size: 737 501 944 829 155 73 69 1101
Cuts at: 6041 7425 7693 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1101 944 829 737 501 268
155 73 69
AfeI AGC'GCT
Cuts at: 3164 4727 3164
Size: 1563 6415
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7781
AflIII A'CryG_T
Cuts at: 1203 2250 6877 7854 1203
Size: 1047 4627 977 1327
Fragments arranged by size:
4627 1327 1047 977
AgeI A'CCGG_T
Cuts at: 2165 6809 2165
Size: 4644 3334
AhdI GACnn_n'nnGTC
Cuts at: 3466 6707 3466
Size: 3241 4737
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7978
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4712 1184
Size: 3528 4450
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 4100 4132 2348
Size: 32 1720 32 6194
Fragments arranged by size:
6194 1720 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2347 3133 3321 3343 3586 3870 3913 4360 5242
Size: 786 188 22 243 284 43 447 882
Cuts at: 5242 5721 7293 2347
Size: 479 1572 3032
Fragments arranged by size:
3032 1572 882 786 479 447 284 243
188 43 22
ApaI G_GGCC'C
Cuts at: 3113 3560 3113
Size: 447 7531
ApaLI G'TGCA_C
Cuts at: 5021 6035 7191 5021
Size: 1014 1156 5808
Fragments arranged by size:
5808 1156 1014
ApoI r'AATT_y
Cuts at: 1493 5287 5513 5665 7774 1493
Size: 3794 226 152 2109 1697
Fragments arranged by size:
3794 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6532 160
Size: 872 5500 1606
Fragments arranged by size:
5500 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7978
AvaI C'yCGr_G
Cuts at: 1870 1943 2393 2447 2641 3158 3487 3560 3809
Size: 73 450 54 194 517 329 73 249
Cuts at: 3809 4733 5000 5303 1870
Size: 924 267 303 4545
Fragments arranged by size:
4545 924 517 450 329 303 267 249
194 73 73 54
BamHI G'GATC_C
Cuts at: 5308 5675 5308
Size: 367 7611
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2298 2645 2810 3788 3881
Size: 325 217 627 511 347 165 978 93
Cuts at: 3881 4876 4929 5146 5299 6754 618
Size: 995 53 217 153 1455 1842
Fragments arranged by size:
1842 1455 995 978 627 511 347 325
217 217 165 153 93 53
BanII G_rGCy'C
Cuts at: 1391 2448 2454 2688 2865 2970 3006 3113 3239
Size: 1057 6 234 177 105 36 107 126
Cuts at: 3239 3560 3678 3963 4260 4374 4443 4950 5297
Size: 321 118 285 297 114 69 507 347
Cuts at: 5297 1391
Size: 4072
Fragments arranged by size:
4072 1057 507 347 321 297 285 234
177 126 118 114 107 105 69 36
6
BbeI G_GCGC'C
Cuts at: 2649 3792 5150 2649
Size: 1143 1358 5477
Fragments arranged by size:
5477 1358 1143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4051 4899 5074 928
Size: 971 2152 848 175 3832
Fragments arranged by size:
3832 2152 971 848 175
BbvCI CC'TCA_GC
Cuts at: 3329 3329
Size: 7978
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4826 4860 6169 6203 1903
Size: 34 2889 34 1309 34 3678
Fragments arranged by size:
3678 2889 1309 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5870 7080 5870
Size: 1210 6768
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7978
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2421 2801 3656
Size: 107 122 71 631 605 749 380 855
Cuts at: 3656 3707 4035 6589 136
Size: 51 328 2554 1525
Fragments arranged by size:
2554 1525 855 749 631 605 380 328
122 107 71 51
BglII A'GATC_T
Cuts at: 7973 7973
Size: 7978
BlpI GC'TnA_GC
Cuts at: 5395 5395
Size: 7978
Bme1580I G_kGCm'C
Cuts at: 1790 3113 3494 3560 3884 4006 4881 5025 6039
Size: 1323 381 66 324 122 875 144 1014
Cuts at: 6039 7195 1790
Size: 1156 2573
Fragments arranged by size:
2573 1323 1156 1014 875 381 324 144
122 66
BmgBI CAC'GTC
Cuts at: 1318 2253 3016 4903 1318
Size: 935 763 1887 4393
Fragments arranged by size:
4393 1887 935 763
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2966 4000 4120
Size: 416 53 83 399 24 1549 1034 120
Cuts at: 4120 5709 6667 442
Size: 1589 958 1753
Fragments arranged by size:
1753 1589 1549 1034 958 416 399 120
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 3077 3197 3223 3892 3938 4033 4207
Size: 1223 615 120 26 669 46 95 174
Cuts at: 4207 4346 4366 4436 4961 6638 1239
Size: 139 20 70 525 1677 2579
Fragments arranged by size:
2579 1677 1223 669 615 525 174 139
120 95 70 46 26 20
Bpu10I CC'TnA_GC
Cuts at: 3170 3329 3384 3170
Size: 159 55 7764
Fragments arranged by size:
7764 159 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 6103 6968 7266 2579
Size: 3524 865 298 3291
Fragments arranged by size:
3524 3291 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6641 882
Size: 617 5142 2219
Fragments arranged by size:
5142 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3205 3223 4237 4318 4560 5275 5769
Size: 711 2001 18 1014 81 242 715 494
Cuts at: 5769 493
Size: 2702
Fragments arranged by size:
2702 2001 1014 715 711 494 242 81
18
BsaBI GATnn'nnATC
Cuts at: 7972 7972
Size: 7978
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2196 2646 3348 3789
Size: 53 83 186 936 663 450 702 441
Cuts at: 3789 5147 5785 6167 6802 275
Size: 1358 638 382 635 1451
Fragments arranged by size:
1451 1358 936 702 663 638 635 450
441 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2165 3356 6411 6809 7083 7230 7641 2165
Size: 1191 3055 398 274 147 411 2502
Fragments arranged by size:
3055 2502 1191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2606 2636 4100 4130 1178
Size: 30 165 30 1203 30 1464 30 5026
Fragments arranged by size:
5026 1464 1203 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2425 2614 2845 2917 2978 3754 3889 4078
Size: 1239 189 231 72 61 776 135 189
Cuts at: 4078 4655 4787 4958 1186
Size: 577 132 171 4206
Fragments arranged by size:
4206 1239 776 577 231 189 189 171
135 132 72 61
BseYI C'CCAG_C
Cuts at: 2687 2777 2892 3057 3314 3747 3756 3852 4043
Size: 90 115 165 257 433 9 96 191
Cuts at: 4043 4434 7181 7677 2687
Size: 391 2747 496 2988
Fragments arranged by size:
2988 2747 496 433 391 257 191 165
115 96 90 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2068 3956 4298 4373 4775 5108 1163
Size: 573 332 1888 342 75 402 333 4033
Fragments arranged by size:
4033 1888 573 402 342 333 332 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2193 2306 3065 3086 3672 4231
Size: 275 186 192 113 759 21 586 559
Cuts at: 4231 5428 6189 7217 1540
Size: 1197 761 1028 2301
Fragments arranged by size:
2301 1197 1028 761 759 586 559 275
192 186 113 21
BsiHKAI G_wGCw'C
Cuts at: 2865 3141 4260 4374 4443 5025 5232 6039 6124
Size: 276 1119 114 69 582 207 807 85
Cuts at: 6124 7195 2865
Size: 1071 3648
Fragments arranged by size:
3648 1119 1071 807 582 276 207 114
85 69
BsmI GAATG_Cn'
Cuts at: 4849 5489 5582 4849
Size: 640 93 7245
Fragments arranged by size:
7245 640 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3934 4088 3934
Size: 154 7824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2448 2454 2688 2865 2970 3006 3113
Size: 399 658 6 234 177 105 36 107
Cuts at: 3113 3141 3239 3494 3560 3678 3884 3963 4006
Size: 28 98 255 66 118 206 79 43
Cuts at: 4006 4260 4374 4443 4881 4950 5025 5232 5297
Size: 254 114 69 438 69 75 207 65
Cuts at: 5297 6039 6124 7195 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 658 438 399 255 254
234 207 206 177 118 114 107 105
98 85 79 75 69 69 66 65
43 36 28 6
BspEI T'CCGG_A
Cuts at: 3356 7641 3356
Size: 4285 3693
BspHI T'CATG_A
Cuts at: 5867 7597 5867
Size: 1730 6248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 3576 4849 5068 5333 844
Size: 1232 1500 1273 219 265 3489
Fragments arranged by size:
3489 1500 1273 1232 265 219
BsrBI CCG'CTC
Cuts at: 1394 1823 2745 2956 3638 5865 1394
Size: 429 922 211 682 2227 3507
Fragments arranged by size:
3507 2227 922 682 429 211
BsrDI GCAATG_nn'
Cuts at: 65 1248 2716 6473 6647 65
Size: 1183 1468 3757 174 1396
Fragments arranged by size:
3757 1468 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2165 2188 2192 2301 3047 3191 3257
Size: 110 355 23 4 109 746 144 66
Cuts at: 3257 3814 4020 4230 4290 4389 4512 5092 6622
Size: 557 206 210 60 99 123 580 1530
Cuts at: 6622 6809 1700
Size: 187 2869
Fragments arranged by size:
2869 1530 746 580 557 355 210 206
187 144 123 110 109 99 66 60
23 4
BsrGI T'GTAC_A
Cuts at: 96 4278 96
Size: 4182 3796
BssHII G'CGCG_C
Cuts at: 1925 2371 3253 1925
Size: 446 882 6650
Fragments arranged by size:
6650 882 446
BssSI C'ACGA_G
Cuts at: 4961 6038 7050 4961
Size: 1077 1012 5889
Fragments arranged by size:
5889 1077 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2476 3136 4360 5029 794
Size: 1682 660 1224 669 3743
Fragments arranged by size:
3743 1682 1224 669 660
BstBI TT'CG_AA
Cuts at: 2007 6782 2007
Size: 4775 3203
BstEII G'GTnAC_C
Cuts at: 1184 2161 4286 4793 5107 1184
Size: 977 2125 507 314 4055
Fragments arranged by size:
4055 2125 977 507 314
BstXI CCAn_nnnn'nTGG
Cuts at: 2711 4180 4456 4888 5143 5257 2711
Size: 1469 276 432 255 114 5432
Fragments arranged by size:
5432 1469 432 276 255 114
BstYI r'GATC_y
Cuts at: 1972 3909 4644 5308 5675 6060 6077 7529 7615
Size: 1937 735 664 367 385 17 1452 86
Cuts at: 7615 7627 7973 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1937 1452 735 664 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2499 3009 3263 3372 4429 5016 5136
Size: 552 1434 510 254 109 1057 587 120
Cuts at: 5136 513
Size: 3355
Fragments arranged by size:
3355 1434 1057 587 552 510 254 120
109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4153 4966 487
Size: 683 56 2927 813 3499
Fragments arranged by size:
3499 2927 813 683 56
BtsI GCAGTG_nn'
Cuts at: 3797 4310 4694 4982 5494 6288 6308 3797
Size: 513 384 288 512 794 20 5467
Fragments arranged by size:
5467 794 513 512 384 288 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7978
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7943
DraI TTT'AAA
Cuts at: 5282 5282
Size: 7978
DraIII CAC_nnn'GTG
Cuts at: 2295 2295
Size: 7978
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4901 5183 6985 809
Size: 507 3585 282 1802 1802
Fragments arranged by size:
3585 1802 1802 507 282
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2303 2331
Size: 54 1425 50 275 18 360 113 28
Cuts at: 2331 2599 3083 3588 3669 4332 4446 5013 5094
Size: 268 484 505 81 663 114 567 81
Cuts at: 5094 5133 5425 6314 7943 8
Size: 39 292 889 1629 43
Fragments arranged by size:
1629 1425 889 663 567 505 484 360
292 275 268 114 113 81 81 54
50 43 39 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 2190 2303 3083 3669 5425 1537
Size: 275 378 113 780 586 1756 4090
Fragments arranged by size:
4090 1756 780 586 378 275 113
EarI CTCTTCn'nnn_
Cuts at: 1353 2542 2846 3500 3917 4154 4265 5908 1353
Size: 1189 304 654 417 237 111 1643 3423
Fragments arranged by size:
3423 1643 1189 654 417 304 237 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2396 4171 4400 6565 6939 7085
Size: 1064 138 807 1775 229 2165 374 146
Cuts at: 7085 387
Size: 1280
Fragments arranged by size:
2165 1775 1280 1064 807 374 229 146
138
EcoICRI GAG'CTC
Cuts at: 2863 4258 4372 4441 2863
Size: 1395 114 69 6400
Fragments arranged by size:
6400 1395 114 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2462 2870 3077 3197 3223 3814
Size: 393 737 93 408 207 120 26 591
Cuts at: 3814 3892 3938 4033 4207 4346 4366 4436 4643
Size: 78 46 95 174 139 20 70 207
Cuts at: 4643 4798 4871 4940 4961 6041 6638 7425 7693
Size: 155 73 69 21 1080 597 787 268
Cuts at: 7693 1239
Size: 1524
Fragments arranged by size:
1524 1080 787 737 597 591 408 393
268 207 207 174 155 139 120 95
93 78 73 70 69 46 26 21
20
EcoNI CCTnn'n_nnAGG
Cuts at: 2561 3590 3686 2561
Size: 1029 96 6853
Fragments arranged by size:
6853 1029 96
EcoO109I rG'GnC_Cy
Cuts at: 2182 2619 2986 3109 3110 3380 3556 3557 3565
Size: 437 367 123 1 270 176 1 8
Cuts at: 3565 3988 4144 4357 4819 2182
Size: 423 156 213 462 5341
Fragments arranged by size:
5341 462 437 423 367 270 213 176
156 123 8 1 1
EcoRI G'AATT_C
Cuts at: 5287 5665 5287
Size: 378 7600
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3916 3916
Size: 7978
FspI TGC'GCA
Cuts at: 4079 6484 4079
Size: 2405 5573
HaeII r_GCGC'y
Cuts at: 1678 2649 3166 3343 3543 3792 3848 4729 4741
Size: 971 517 177 200 249 56 881 12
Cuts at: 4741 4787 5150 7125 1678
Size: 46 363 1975 2531
Fragments arranged by size:
2531 1975 971 881 517 363 249 200
177 56 46 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3425 3452 3457 3484 6619
Size: 32 135 32 1853 27 5 27 3135
Cuts at: 6619 6651 6693 6725 1373
Size: 32 42 32 2626
Fragments arranged by size:
3135 2626 1853 135 42 32 32 32
32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2065 5322 5568 669
Size: 988 281 127 3257 246 3079
Fragments arranged by size:
3257 3079 988 281 246 127
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7978
HpaI GTT'AAC
Cuts at: 5568 5568
Size: 7978
KasI G'GCGC_C
Cuts at: 2645 3788 5146 2645
Size: 1143 1358 5477
Fragments arranged by size:
5477 1358 1143
KpnI G_GTAC'C
Cuts at: 4933 5303 4933
Size: 370 7608
MfeI C'AATT_G
Cuts at: 5577 5577
Size: 7978
MluI A'CGCG_T
Cuts at: 7854 7854
Size: 7978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 5196 7092 7276 1305
Size: 780 3111 1896 184 2007
Fragments arranged by size:
3111 2007 1896 780 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 4334 4448 5135 10
Size: 54 1425 343 501 2001 114 687 2853
Fragments arranged by size:
2853 2001 1425 687 501 343 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3014 4217 4712 5114 5141 5936 6295
Size: 648 1848 1203 495 402 27 795 359
Cuts at: 6295 6454 518
Size: 159 2042
Fragments arranged by size:
2042 1848 1203 795 648 495 402 359
159 27
MspA1I CmG'CkG
Cuts at: 1606 2329 2501 2529 2884 3061 3133 3214 3265
Size: 723 172 28 355 177 72 81 51
Cuts at: 3265 3415 3747 4035 4053 4384 4431 4744 5394
Size: 150 332 288 18 331 47 313 650
Cuts at: 5394 6071 7219 7464 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 723 677 650 355 332 331
313 288 245 177 172 150 81 72
51 47 28 18
NaeI GCC'GGC
Cuts at: 2190 2194 3049 3193 3259 4022 4391 5094 2190
Size: 4 855 144 66 763 369 703 5074
Fragments arranged by size:
5074 855 763 703 369 144 66 4
NarI GG'CG_CC
Cuts at: 2646 3789 5147 2646
Size: 1143 1358 5477
Fragments arranged by size:
5477 1358 1143
NcoI C'CATG_G
Cuts at: 513 1065 3009 3372 5136 513
Size: 552 1944 363 1764 3355
Fragments arranged by size:
3355 1944 1764 552 363
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7978
NgoMIV G'CCGG_C
Cuts at: 2188 2192 3047 3191 3257 4020 4389 5092 2188
Size: 4 855 144 66 763 369 703 5074
Fragments arranged by size:
5074 855 763 703 369 144 66 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7978
NotI GC'GGCC_GC
Cuts at: 5425 5425
Size: 7978
NspI r_CATG'y
Cuts at: 1132 1229 2149 2662 4180 4655 4717 5336 6881
Size: 97 920 513 1518 475 62 619 1545
Cuts at: 6881 1132
Size: 2229
Fragments arranged by size:
2229 1545 1518 920 619 513 475 97
62
PasI CC'CwG_GG
Cuts at: 1348 2704 2867 4749 4823 1348
Size: 1356 163 1882 74 4503
Fragments arranged by size:
4503 1882 1356 163 74
PciI A'CATG_T
Cuts at: 6877 6877
Size: 7978
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3133 3343 3444 4344 5242 1108
Size: 2025 210 101 900 898 3844
Fragments arranged by size:
3844 2025 900 898 210 101
PmeI GTTT'AAAC
Cuts at: 5282 5282
Size: 7978
PmlI CAC'GTG
Cuts at: 4237 4318 4237
Size: 81 7897
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2940 2972 4100 4132 6047 6079 7560 7592 2940
Size: 32 1128 32 1915 32 1481 32 3326
Fragments arranged by size:
3326 1915 1481 1128 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2182 2619 3380 3565 3988 4819 2182
Size: 437 761 185 423 831 5341
Fragments arranged by size:
5341 831 761 437 423 185
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7978
PsiI TTA'TAA
Cuts at: 5548 5548
Size: 7978
PspOMI G'GGCC_C
Cuts at: 3109 3556 3109
Size: 447 7531
PstI C_TGCA'G
Cuts at: 830 2343 2787 4701 4860 5079 5330 830
Size: 1513 444 1914 159 219 251 3478
Fragments arranged by size:
3478 1914 1513 444 251 219 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7978
PvuII CAG'CTG
Cuts at: 3133 3214 3747 4053 4384 4744 3133
Size: 81 533 306 331 360 6367
Fragments arranged by size:
6367 533 360 331 306 81
SacI G_AGCT'C
Cuts at: 2865 4260 4374 4443 2865
Size: 1395 114 69 6400
Fragments arranged by size:
6400 1395 114 69
SacII CC_GC'GG
Cuts at: 2502 3266 4432 2502
Size: 764 1166 6048
Fragments arranged by size:
6048 1166 764
SalI G'TCGA_C
Cuts at: 1936 2063 5320 1936
Size: 127 3257 4594
Fragments arranged by size:
4594 3257 127
SanDI GG'GwC_CC
Cuts at: 2182 4819 2182
Size: 2637 5341
SapI GCTCTTCn'nnn_
Cuts at: 2542 3500 3917 4265 2542
Size: 958 417 348 6255
Fragments arranged by size:
6255 958 417 348
SbfI CC_TGCA'GG
Cuts at: 5079 5330 5079
Size: 251 7727
ScaI AGT'ACT
Cuts at: 4571 6226 7938 4571
Size: 1655 1712 4611
Fragments arranged by size:
4611 1712 1655
SexAI A'CCwGG_T
Cuts at: 2157 2157
Size: 7978
SfcI C'TryA_G
Cuts at: 826 1045 2339 2614 2783 3364 4586 4697 4856
Size: 219 1294 275 169 581 1222 111 159
Cuts at: 4856 5075 5326 6461 7142 7333 826
Size: 219 251 1135 681 191 1471
Fragments arranged by size:
1471 1294 1222 1135 681 581 275 251
219 219 191 169 159 111
SfoI GGC'GCC
Cuts at: 2647 3790 5148 2647
Size: 1143 1358 5477
Fragments arranged by size:
5477 1358 1143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7978
SgrAI Cr'CCGG_yG
Cuts at: 1700 2165 1700
Size: 465 7513
SmaI CCC'GGG
Cuts at: 1872 2395 3489 3562 3811 5305 1872
Size: 523 1094 73 249 1494 4545
Fragments arranged by size:
4545 1494 1094 523 249 73
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 6082 6983 7245 820
Size: 197 926 615 3524 901 262 1553
Fragments arranged by size:
3524 1553 926 901 615 262 197
SnaBI TAC'GTA
Cuts at: 493 5275 5769 493
Size: 4782 494 2702
Fragments arranged by size:
4782 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7978
SphI G_CATG'C
Cuts at: 1132 2662 4655 4717 5336 1132
Size: 1530 1993 62 619 3774
Fragments arranged by size:
3774 1993 1530 619 62
SrfI GCCC'GGGC
Cuts at: 3811 3811
Size: 7978
SspI AAT'ATT
Cuts at: 5 52 5902 5
Size: 47 5850 2081
Fragments arranged by size:
5850 2081 47
StuI AGG'CCT
Cuts at: 1517 4250 4887 1517
Size: 2733 637 4608
Fragments arranged by size:
4608 2733 637
StyI C'CwwG_G
Cuts at: 513 1065 3009 3372 4299 4338 5136 5417 513
Size: 552 1944 363 927 39 798 281 3074
Fragments arranged by size:
3074 1944 927 798 552 363 281 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2101 2791 4274 4741 6005 6022 6175
Size: 1255 521 690 1483 467 1264 17 153
Cuts at: 6175 6360 6805 325
Size: 185 445 1498
Fragments arranged by size:
1498 1483 1264 1255 690 521 467 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3180 4278 4569
Size: 274 80 33 51 1443 1203 1098 291
Cuts at: 4569 6224 7936 96
Size: 1655 1712 138
Fragments arranged by size:
1712 1655 1443 1203 1098 291 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2030 2062 2724 2756 7938 7970 2030
Size: 32 662 32 5182 32 2038
Fragments arranged by size:
5182 2038 662 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4526 1316
Size: 3210 4768
XbaI T'CTAG_A
Cuts at: 5314 5314
Size: 7978
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2437 2437
Size: 7978
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7978
XmaI C'CCGG_G
Cuts at: 1870 2393 3487 3560 3809 5303 1870
Size: 523 1094 73 249 1494 4545
Fragments arranged by size:
4545 1494 1094 523 249 73
XmnI GAAnn'nnTTC
Cuts at: 6107 6107
Size: 7978
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5786 276
Size: 53 83 186 936 4252 2468
Fragments arranged by size:
4252 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI
PsrI RsrII SfiI SwaI