Restriction map for
pFN21ASDA1686
(Circular) (Six-base) MAPSORT of: pFN21ASDA1686.seq Check: 5495 from: 1 to: 8251
pFN21ASDA1686 8251 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6061 278
Size: 53 83 186 936 4525 2468
Fragments arranged by size:
4525 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5594 1937
Size: 3657 4594
Acc65I G'GTAC_C
Cuts at: 5572 5572
Size: 8251
AclI AA'CG_TT
Cuts at: 1563 6378 6751 1563
Size: 4815 373 3063
Fragments arranged by size:
4815 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2376 2400 2721 3408 4107 4148 4689 4968
Size: 744 24 321 687 699 41 541 279
Cuts at: 4968 5155 5376 6314 7698 7966 1632
Size: 187 221 938 1384 268 1917
Fragments arranged by size:
1917 1384 938 744 699 687 541 321
279 268 221 187 41 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8054
AflIII A'CryG_T
Cuts at: 1203 4307 7150 8127 1203
Size: 3104 2843 977 1327
Fragments arranged by size:
3104 2843 1327 977
AgeI A'CCGG_T
Cuts at: 2527 7082 2527
Size: 4555 3696
AhdI GACnn_n'nnGTC
Cuts at: 3805 6980 3805
Size: 3175 5076
AleI CACnn'nnGTG
Cuts at: 1166 4425 1166
Size: 3259 4992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2210 1184
Size: 1026 7225
AlwNI CAG_nnn'CTG
Cuts at: 2840 4685 5994 7566 2840
Size: 1845 1309 1572 3525
Fragments arranged by size:
3525 1845 1572 1309
ApaI G_GGCC'C
Cuts at: 3210 3772 3210
Size: 562 7689
ApaLI G'TGCA_C
Cuts at: 3113 4461 6308 7464 3113
Size: 1348 1847 1156 3900
Fragments arranged by size:
3900 1847 1348 1156
ApoI r'AATT_y
Cuts at: 1493 2500 3061 3247 3993 4452 4488 5014 5242
Size: 1007 561 186 746 459 36 526 228
Cuts at: 5242 5560 5786 5938 8047 1493
Size: 318 226 152 2109 1697
Fragments arranged by size:
2109 1697 1007 746 561 526 459 318
228 226 186 152 36
AseI AT'TA_AT
Cuts at: 160 1032 2712 3045 6805 160
Size: 872 1680 333 3760 1606
Fragments arranged by size:
3760 1680 1606 872 333
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8251
AvaI C'yCGr_G
Cuts at: 1870 1943 4323 5576 1870
Size: 73 2380 1253 4545
Fragments arranged by size:
4545 2380 1253 73
BamHI G'GATC_C
Cuts at: 5581 5948 5581
Size: 367 7884
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5572 7027 618
Size: 325 217 627 3785 1455 1842
Fragments arranged by size:
3785 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2444 3210 3367 3772 5570 1391
Size: 1053 766 157 405 1798 4072
Fragments arranged by size:
4072 1798 1053 766 405 157
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2092 3714 4933 928
Size: 971 193 1622 1219 4246
Fragments arranged by size:
4246 1622 1219 971 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6442 6476 1903
Size: 34 4505 34 3678
Fragments arranged by size:
4505 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2660 3680 3938 6143 7353 2660
Size: 1020 258 2205 1210 3558
Fragments arranged by size:
3558 2205 1210 1020 258
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8251
BfrBI ATG'CAT
Cuts at: 4234 4234
Size: 8251
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2448 6862 136
Size: 107 122 71 631 605 776 4414 1525
Fragments arranged by size:
4414 1525 776 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3175 8246 3175
Size: 5071 3180
BlpI GC'TnA_GC
Cuts at: 5668 5668
Size: 8251
Bme1580I G_kGCm'C
Cuts at: 1790 3117 3210 3772 4465 6312 7468 1790
Size: 1327 93 562 693 1847 1156 2573
Fragments arranged by size:
2573 1847 1327 1156 693 562 93
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8251
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5982 6940 442
Size: 416 53 83 399 24 4565 958 1753
Fragments arranged by size:
4565 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8251
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 8251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 6911 1239
Size: 824 4848 2579
Fragments arranged by size:
4848 2579 824
Bpu10I CC'TnA_GC
Cuts at: 3780 5226 3780
Size: 1446 6805
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3154 3989 4214 4822 5295 6376 7241 7539 3154
Size: 835 225 608 473 1081 865 298 3866
Fragments arranged by size:
3866 1081 865 835 608 473 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6914 882
Size: 617 5415 2219
Fragments arranged by size:
5415 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4308 5548 6042 493
Size: 711 3104 1240 494 2702
Fragments arranged by size:
3104 2702 1240 711 494
BsaBI GATnn'nnATC
Cuts at: 3278 8245 3278
Size: 4967 3284
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6058 6440 7075 275
Size: 53 83 186 936 4525 382 635 1451
Fragments arranged by size:
4525 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2168 2527 6684 7082 7356 7503 7914 2168
Size: 359 4157 398 274 147 411 2505
Fragments arranged by size:
4157 2505 411 398 359 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3926 3956 3993 4023 1178
Size: 30 165 30 2523 30 37 30 5406
Fragments arranged by size:
5406 2523 165 37 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 3503 3753 3887 1186
Size: 943 1374 250 134 5550
Fragments arranged by size:
5550 1374 943 250 134
BseYI C'CCAG_C
Cuts at: 2205 4246 7454 7950 2205
Size: 2041 3208 496 2506
Fragments arranged by size:
3208 2506 2041 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 2752 4872 1163
Size: 573 387 629 2120 4542
Fragments arranged by size:
4542 2120 629 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2095 3229 5701 6462 7490 1540
Size: 275 186 94 1134 2472 761 1028 2301
Fragments arranged by size:
2472 2301 1134 1028 761 275 186 94
BsiHKAI G_wGCw'C
Cuts at: 2129 2189 3117 3572 4465 6312 6397 7468 2129
Size: 60 928 455 893 1847 85 1071 2912
Fragments arranged by size:
2912 1847 1071 928 893 455 85 60
BsmI GAATG_Cn'
Cuts at: 5762 5855 5762
Size: 93 8158
BsmBI CGTCTCn'nnnn_
Cuts at: 3895 3895
Size: 8251
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2189 2444 3117 3210 3367 3572
Size: 399 339 60 255 673 93 157 205
Cuts at: 3572 3772 4465 5570 6312 6397 7468 1391
Size: 200 693 1105 742 85 1071 2174
Fragments arranged by size:
2174 1105 1071 742 693 673 399 339
255 205 200 157 93 85 60
BspEI T'CCGG_A
Cuts at: 7914 7914
Size: 8251
BspHI T'CATG_A
Cuts at: 3298 3460 6140 7870 3298
Size: 162 2680 1730 3679
Fragments arranged by size:
3679 2680 1730 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2279 5606 844
Size: 1435 3327 3489
Fragments arranged by size:
3489 3327 1435
BsrBI CCG'CTC
Cuts at: 1394 1823 3485 6138 1394
Size: 429 1662 2653 3507
Fragments arranged by size:
3507 2653 1662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6746 6920 65
Size: 1183 5498 174 1396
Fragments arranged by size:
5498 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2150 2175 2527 6895 7082 1700
Size: 110 340 25 352 4368 187 2869
Fragments arranged by size:
4368 2869 352 340 187 110 25
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8251
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8251
BssSI C'ACGA_G
Cuts at: 2460 6311 7323 2460
Size: 3851 1012 3388
Fragments arranged by size:
3851 3388 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3101 4204 794
Size: 2307 1103 4841
Fragments arranged by size:
4841 2307 1103
BstBI TT'CG_AA
Cuts at: 2007 7055 2007
Size: 5048 3203
BstEII G'GTnAC_C
Cuts at: 1184 2164 1184
Size: 980 7271
BstXI CCAn_nnnn'nTGG
Cuts at: 3610 4030 3610
Size: 420 7831
BstYI r'GATC_y
Cuts at: 1972 2032 2049 2931 3175 4825 5581 5948 6333
Size: 60 17 882 244 1650 756 367 385
Cuts at: 6333 6350 7802 7888 7900 8246 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1650 1452 882 756 385 367 346
244 86 60 17 17 12
Bsu36I CC'TnA_GG
Cuts at: 4946 5289 5421 4946
Size: 343 132 7776
Fragments arranged by size:
7776 343 132
BtgI C'CryG_G
Cuts at: 513 1065 2022 3609 3661 513
Size: 552 957 1587 52 5103
Fragments arranged by size:
5103 1587 957 552 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5472 487
Size: 683 56 4246 3266
Fragments arranged by size:
4246 3266 683 56
BtsI GCAGTG_nn'
Cuts at: 2433 3448 4329 4860 5076 5457 5767 6561 6581
Size: 1015 881 531 216 381 310 794 20
Cuts at: 6581 2433
Size: 4103
Fragments arranged by size:
4103 1015 881 794 531 381 310 216
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8251
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8216
DraI TTT'AAA
Cuts at: 5555 5555
Size: 8251
DraIII CAC_nnn'GTG
Cuts at: 3522 3522
Size: 8251
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3832 7258 809
Size: 507 2516 3426 1802
Fragments arranged by size:
3426 2516 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 2372 5698
Size: 54 1425 50 275 18 262 280 3326
Cuts at: 5698 6587 8216 8
Size: 889 1629 43
Fragments arranged by size:
3326 1629 1425 889 280 275 262 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2092 5698 1537
Size: 275 280 3606 4090
Fragments arranged by size:
4090 3606 280 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2637 4149 4899 5340 6181 1353
Size: 1284 1512 750 441 841 3423
Fragments arranged by size:
3423 1512 1284 841 750 441
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2045 6838 7212 7358 387
Size: 1064 138 456 4793 374 146 1280
Fragments arranged by size:
4793 1280 1064 456 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2376 2400 2721 3408 4107 4148
Size: 393 431 313 24 321 687 699 41
Cuts at: 4148 4689 4968 5155 5376 6314 6911 7698 7966
Size: 541 279 187 221 938 597 787 268
Cuts at: 7966 1239
Size: 1524
Fragments arranged by size:
1524 938 787 699 687 597 541 431
393 321 313 279 268 221 187 41
24
EcoO109I rG'GnC_Cy
Cuts at: 3206 3207 3476 3768 3206
Size: 1 269 292 7689
Fragments arranged by size:
7689 292 269 1
EcoRI G'AATT_C
Cuts at: 3061 4452 4488 5560 5938 3061
Size: 1391 36 1072 378 5374
Fragments arranged by size:
5374 1391 1072 378 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4178 4178
Size: 8251
FspI TGC'GCA
Cuts at: 6757 6757
Size: 8251
HaeII r_GCGC'y
Cuts at: 1678 7398 1678
Size: 5720 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3074 3106 3791 3823 4810
Size: 32 135 32 1502 32 685 32 987
Cuts at: 4810 4842 5317 5349 6892 6924 6966 6998 1373
Size: 32 475 32 1543 32 42 32 2626
Fragments arranged by size:
2626 1543 1502 987 685 475 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4635 5380 5595 5841 669
Size: 988 281 2697 745 215 246 3079
Fragments arranged by size:
3079 2697 988 745 281 246 215
HindIII A'AGCT_T
Cuts at: 748 2394 3965 4200 748
Size: 1646 1571 235 4799
Fragments arranged by size:
4799 1646 1571 235
HpaI GTT'AAC
Cuts at: 4635 5841 4635
Size: 1206 7045
KpnI G_GTAC'C
Cuts at: 5576 5576
Size: 8251
MfeI C'AATT_G
Cuts at: 2308 5850 2308
Size: 3542 4709
MluI A'CGCG_T
Cuts at: 8127 8127
Size: 8251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3243 4714 7365 7549 1305
Size: 1938 1471 2651 184 2007
Fragments arranged by size:
2651 2007 1938 1471 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6429
Fragments arranged by size:
6429 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3789 4425 5540 6209 6568 6727 518
Size: 648 2623 636 1115 669 359 159 2042
Fragments arranged by size:
2623 2042 1115 669 648 636 359 159
MspA1I CmG'CkG
Cuts at: 1606 3818 4042 4414 5667 6344 7492 7737 1606
Size: 2212 224 372 1253 677 1148 245 2120
Fragments arranged by size:
2212 2120 1253 1148 677 372 245 224
NcoI C'CATG_G
Cuts at: 513 1065 2022 3609 513
Size: 552 957 1587 5155
Fragments arranged by size:
5155 1587 957 552
NdeI CA'TA_TG
Cuts at: 387 5206 387
Size: 4819 3432
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8251
NotI GC'GGCC_GC
Cuts at: 5698 5698
Size: 8251
NsiI A_TGCA'T
Cuts at: 4236 4236
Size: 8251
NspI r_CATG'y
Cuts at: 1132 1229 2752 3099 3788 4148 4238 5609 7154
Size: 97 1523 347 689 360 90 1371 1545
Cuts at: 7154 1132
Size: 2229
Fragments arranged by size:
2229 1545 1523 1371 689 360 347 97
90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8251
PciI A'CATG_T
Cuts at: 7150 7150
Size: 8251
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3373 5164 1108
Size: 2265 1791 4195
Fragments arranged by size:
4195 2265 1791
PfoI T'CCnGG_A
Cuts at: 2066 3930 2066
Size: 1864 6387
PmeI GTTT'AAAC
Cuts at: 5555 5555
Size: 8251
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6320 6352 7833 7865 6320
Size: 32 1481 32 6706
Fragments arranged by size:
6706 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3476 3476
Size: 8251
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8251
PsiI TTA'TAA
Cuts at: 2553 3558 5821 2553
Size: 1005 2263 4983
Fragments arranged by size:
4983 2263 1005
PspOMI G'GGCC_C
Cuts at: 3206 3768 3206
Size: 562 7689
PstI C_TGCA'G
Cuts at: 830 3443 5603 830
Size: 2613 2160 3478
Fragments arranged by size:
3478 2613 2160
PvuI CG_AT'CG
Cuts at: 2001 3229 2001
Size: 1228 7023
PvuII CAG'CTG
Cuts at: 3818 4042 4414 3818
Size: 224 372 7655
Fragments arranged by size:
7655 372 224
SalI G'TCGA_C
Cuts at: 1936 5593 1936
Size: 3657 4594
SapI GCTCTTCn'nnn_
Cuts at: 4899 5340 4899
Size: 441 7810
SbfI CC_TGCA'GG
Cuts at: 5603 5603
Size: 8251
ScaI AGT'ACT
Cuts at: 2566 3589 4882 6499 8211 2566
Size: 1023 1293 1617 1712 2606
Fragments arranged by size:
2606 1712 1617 1293 1023
SfcI C'TryA_G
Cuts at: 826 1045 2445 3118 3439 4539 5599 6734 7415
Size: 219 1400 673 321 1100 1060 1135 681
Cuts at: 7415 7606 826
Size: 191 1471
Fragments arranged by size:
1471 1400 1135 1100 1060 681 673 321
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8251
SgrAI Cr'CCGG_yG
Cuts at: 1700 2150 2175 1700
Size: 450 25 7776
Fragments arranged by size:
7776 450 25
SmaI CCC'GGG
Cuts at: 1872 5578 1872
Size: 3706 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3133 3968 4193 4323 4801 5310
Size: 197 926 1190 835 225 130 478 509
Cuts at: 5310 6355 7256 7518 820
Size: 1045 901 262 1553
Fragments arranged by size:
1553 1190 1045 926 901 835 509 478
262 225 197 130
SnaBI TAC'GTA
Cuts at: 493 5548 6042 493
Size: 5055 494 2702
Fragments arranged by size:
5055 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8251
SphI G_CATG'C
Cuts at: 1132 3099 4148 4238 5609 1132
Size: 1967 1049 90 1371 3774
Fragments arranged by size:
3774 1967 1371 1049 90
SspI AAT'ATT
Cuts at: 5 52 3619 6175 5
Size: 47 3567 2556 2081
Fragments arranged by size:
3567 2556 2081 47
StuI AGG'CCT
Cuts at: 1517 3427 1517
Size: 1910 6341
StyI C'CwwG_G
Cuts at: 513 1065 2022 3609 3856 4423 4889 5690 513
Size: 552 957 1587 247 567 466 801 3074
Fragments arranged by size:
3074 1587 957 801 567 552 466 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6278 6295 6448 6633 7078 325
Size: 1255 4698 17 153 185 445 1498
Fragments arranged by size:
4698 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2564 2827 3587
Size: 274 80 33 51 1443 587 263 760
Cuts at: 3587 4584 4880 6497 8209 96
Size: 997 296 1617 1712 138
Fragments arranged by size:
1712 1617 1443 997 760 587 296 274
263 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3993 4025 8211 8243 3993
Size: 32 4186 32 4001
Fragments arranged by size:
4186 4001 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8251
XbaI T'CTAG_A
Cuts at: 5429 5587 5429
Size: 158 8093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4261 4336 4730 4261
Size: 75 394 7782
Fragments arranged by size:
7782 394 75
XhoI C'TCGA_G
Cuts at: 1943 4323 1943
Size: 2380 5871
XmaI C'CCGG_G
Cuts at: 1870 5576 1870
Size: 3706 4545
XmnI GAAnn'nnTTC
Cuts at: 3247 5133 6380 3247
Size: 1886 1247 5118
Fragments arranged by size:
5118 1886 1247
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6059 276
Size: 53 83 186 936 4525 2468
Fragments arranged by size:
4525 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI