Restriction map for
pFN21ASDA1674
(Circular) (Six-base) MAPSORT of: pFN21ASDA1674.seq Check: 4132 from: 1 to: 6319
pFN21ASDA1674 6319 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3502 3662 1937
Size: 1565 160 4594
Fragments arranged by size:
4594 1565 160
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4382 5766 6034 1632
Size: 2750 1384 268 1917
Fragments arranged by size:
2750 1917 1384 268
AfeI AGC'GCT
Cuts at: 2337 2519 2337
Size: 182 6137
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 2646 2938 5218 6195 1203
Size: 1443 292 2280 977 1327
Fragments arranged by size:
2280 1443 1327 977 292
AgeI A'CCGG_T
Cuts at: 2584 5150 2584
Size: 2566 3753
AhdI GACnn_n'nnGTC
Cuts at: 2647 2989 5048 2647
Size: 342 2059 3918
Fragments arranged by size:
3918 2059 342
AleI CACnn'nnGTG
Cuts at: 1166 2133 1166
Size: 967 5352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2268 3188 1184
Size: 1084 920 4315
Fragments arranged by size:
4315 1084 920
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2535 2567 2535
Size: 32 6287
AlwNI CAG_nnn'CTG
Cuts at: 3076 4062 5634 3076
Size: 986 1572 3761
Fragments arranged by size:
3761 1572 986
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 2471 3328 3628 3854 4006 6115 1493
Size: 978 857 300 226 152 2109 1697
Fragments arranged by size:
2109 1697 978 857 300 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2614 3644 1870
Size: 73 671 1030 4545
Fragments arranged by size:
4545 1030 671 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3640 5095 618
Size: 325 217 627 1853 1455 1842
Fragments arranged by size:
1853 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2359 2621 3060 3133 3162 3638 1391
Size: 968 262 439 73 29 476 4072
Fragments arranged by size:
4072 968 476 439 262 73 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2960 928
Size: 971 1061 4287
Fragments arranged by size:
4287 1061 971
BbvCI CC'TCA_GC
Cuts at: 2481 2816 2481
Size: 335 5984
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4930 136
Size: 107 122 71 631 605 3258 1525
Fragments arranged by size:
3258 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3220 6314 3220
Size: 3094 3225
BlpI GC'TnA_GC
Cuts at: 3161 3736 3161
Size: 575 5744
Bme1580I G_kGCm'C
Cuts at: 1790 3109 3316 4380 5536 1790
Size: 1319 207 1064 1156 2573
Fragments arranged by size:
2573 1319 1156 1064 207
BmgBI CAC'GTC
Cuts at: 1318 3355 1318
Size: 2037 4282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4050 5008 442
Size: 416 53 83 399 24 2633 958 1753
Fragments arranged by size:
2633 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2882
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2648 2658 2950 3044 3422 4979 1239
Size: 854 555 10 292 94 378 1557 2579
Fragments arranged by size:
2579 1557 854 555 378 292 94 10
Bpu10I CC'TnA_GC
Cuts at: 2481 2673 2816 2481
Size: 192 143 5984
Fragments arranged by size:
5984 192 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2197 2256 2556 2879 4444 5309 5607 2197
Size: 59 300 323 1565 865 298 2909
Fragments arranged by size:
2909 1565 865 323 300 298 59
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2634 4982 882
Size: 617 1135 2348 2219
Fragments arranged by size:
2348 2219 1135 617
BsaAI yAC'GTr
Cuts at: 493 1204 2839 3616 4110 493
Size: 711 1635 777 494 2702
Fragments arranged by size:
2702 1635 777 711 494
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4126 4508 5143 275
Size: 53 83 186 936 2593 382 635 1451
Fragments arranged by size:
2593 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2316 2584 4752 5150 5424 5571 5982 2316
Size: 268 2168 398 274 147 411 2653
Fragments arranged by size:
2653 2168 411 398 274 268 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2535 2565 1178
Size: 30 165 30 1132 30 4932
Fragments arranged by size:
4932 1132 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2149 2219 2469 2758 2804 2807 2953
Size: 904 59 70 250 289 46 3 146
Cuts at: 2953 3199 3419 1186
Size: 246 220 4086
Fragments arranged by size:
4086 904 289 250 246 220 146 70
59 46 3
BseYI C'CCAG_C
Cuts at: 2222 2419 2783 5522 6018 2222
Size: 197 364 2739 496 2523
Fragments arranged by size:
2739 2523 496 364 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2513 1163
Size: 573 777 4969
Fragments arranged by size:
4969 777 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2359 2838 3060 3162 4380 4465 5536 2359
Size: 479 222 102 1218 85 1071 3142
Fragments arranged by size:
3142 1218 1071 479 222 102 85
BsmI GAATG_Cn'
Cuts at: 2257 3830 3923 2257
Size: 1573 93 4653
Fragments arranged by size:
4653 1573 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3085 3085
Size: 6319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2359 2621 2838 3060 3109 3133 3162
Size: 399 569 262 217 222 49 24 29
Cuts at: 3162 3316 3638 4380 4465 5536 1391
Size: 154 322 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 399 322 262 222
217 154 85 49 29 24
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3674 844
Size: 2830 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3041 4206 1394
Size: 429 1218 1165 3507
Fragments arranged by size:
3507 1218 1165 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4814 4988 65
Size: 1183 3566 174 1396
Fragments arranged by size:
3566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2584 3541 4963 5150 1700
Size: 110 774 957 1422 187 2869
Fragments arranged by size:
2869 1422 957 774 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6319
BstBI TT'CG_AA
Cuts at: 2007 5123 2007
Size: 3116 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 3220 3427 3649 4016 4401 4418 5870 5956
Size: 1248 207 222 367 385 17 1452 86
Cuts at: 5956 5968 6314 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1248 385 367 346 222 207
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3179 3385 513
Size: 552 957 1157 206 3447
Fragments arranged by size:
3447 1157 957 552 206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2458 3107 3835 4629 4649 2458
Size: 649 728 794 20 4128
Fragments arranged by size:
4128 794 728 649 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3388 3766 4655
Size: 54 1425 50 275 18 1558 378 889
Cuts at: 4655 6284 8
Size: 1629 43
Fragments arranged by size:
1629 1558 1425 889 378 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3024 3140 3150 3330 4249 1353
Size: 1671 116 10 180 919 3423
Fragments arranged by size:
3423 1671 919 180 116 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3243 4906 5280 5426 387
Size: 1064 138 1654 1663 374 146 1280
Fragments arranged by size:
1663 1654 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2357 3058 3160 2357
Size: 701 102 5516
Fragments arranged by size:
5516 701 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2648 2658 2950 3044 3422 4382
Size: 393 461 555 10 292 94 378 960
Cuts at: 4382 4979 5766 6034 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 960 787 597 555 461 393 378
292 268 94 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2965 2965
Size: 6319
EcoO109I rG'GnC_Cy
Cuts at: 2677 3394 2677
Size: 717 5602
EcoRI G'AATT_C
Cuts at: 2471 3628 4006 2471
Size: 1157 378 4784
Fragments arranged by size:
4784 1157 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 2920 2728
Size: 192 6127
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2339 2521 2806 3050 5466 1678
Size: 661 182 285 244 2416 2531
Fragments arranged by size:
2531 2416 661 285 244 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2133 2165 2341 2373 2633
Size: 32 135 32 561 32 176 32 260
Cuts at: 2633 2665 2975 3007 4960 4992 5034 5066 1373
Size: 32 310 32 1953 32 42 32 2626
Fragments arranged by size:
2626 1953 561 310 260 176 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3503 3663 3909 669
Size: 988 281 1565 160 246 3079
Fragments arranged by size:
3079 1565 988 281 246 160
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 2234 3918 2234
Size: 1684 4635
MluI A'CGCG_T
Cuts at: 2646 6195 2646
Size: 3549 2770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2119 2588 5433 5617 1305
Size: 814 469 2845 184 2007
Fragments arranged by size:
2845 2007 814 469 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3390 10
Size: 54 1425 343 1558 2939
Fragments arranged by size:
2939 1558 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2133 4277 4636 4795 518
Size: 648 967 2144 359 159 2042
Fragments arranged by size:
2144 2042 967 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3181 3735 4412 5560 5805 1606
Size: 1575 554 677 1148 245 2120
Fragments arranged by size:
2120 1575 1148 677 554 245
NaeI GCC'GGC
Cuts at: 3543 3543
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2022 3385 513
Size: 552 957 1363 3447
Fragments arranged by size:
3447 1363 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 3541 3541
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 3677 5222 1132
Size: 97 2448 1545 2229
Fragments arranged by size:
2448 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6319
PfoI T'CCnGG_A
Cuts at: 2966 3060 2966
Size: 94 6225
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PmlI CAC'GTG
Cuts at: 2839 2839
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2535 2567 4388 4420 5901 5933 2535
Size: 32 1821 32 1481 32 2921
Fragments arranged by size:
2921 1821 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2677 2677
Size: 6319
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PstI C_TGCA'G
Cuts at: 830 3114 3321 3671 830
Size: 2284 207 350 3478
Fragments arranged by size:
3478 2284 350 207
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
SacI G_AGCT'C
Cuts at: 2359 3060 3162 2359
Size: 701 102 5516
Fragments arranged by size:
5516 701 102
SacII CC_GC'GG
Cuts at: 3182 3182
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3501 3661 1936
Size: 1565 160 4594
Fragments arranged by size:
4594 1565 160
SapI GCTCTTCn'nnn_
Cuts at: 3150 3150
Size: 6319
SbfI CC_TGCA'GG
Cuts at: 3321 3671 3321
Size: 350 5969
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SfcI C'TryA_G
Cuts at: 826 1045 3110 3317 3449 3667 4802 5483 5674
Size: 219 2065 207 132 218 1135 681 191
Cuts at: 5674 826
Size: 1471
Fragments arranged by size:
2065 1471 1135 681 219 218 207 191
132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3646 1872
Size: 1774 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2176 2271 2571 2894 4423 5324
Size: 197 926 233 95 300 323 1529 901
Cuts at: 5324 5586 820
Size: 262 1553
Fragments arranged by size:
1553 1529 926 901 323 300 262 233
197 95
SnaBI TAC'GTA
Cuts at: 493 3616 4110 493
Size: 3123 494 2702
Fragments arranged by size:
3123 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6319
StyI C'CwwG_G
Cuts at: 513 1065 2022 2656 3385 3758 513
Size: 552 957 634 729 373 3074
Fragments arranged by size:
3074 957 729 634 552 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4346 4363 4516 4701 5146 325
Size: 1255 2766 17 153 185 445 1498
Fragments arranged by size:
2766 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4565 6277 96
Size: 274 80 33 51 1443 2588 1712 138
Fragments arranged by size:
2588 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 3644 1870
Size: 1774 4545
XmnI GAAnn'nnTTC
Cuts at: 3526 4448 3526
Size: 922 5397
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII
RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI