Restriction map for
pFN21ASDA1665
(Circular) (Six-base) MAPSORT of: pFN21ASDA1665.seq Check: 6157 from: 1 to: 5341
pFN21ASDA1665 5341 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2386 2386
Size: 5341
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2684 1937
Size: 747 4594
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3404 4788 5056 1632
Size: 1772 1384 268 1917
Fragments arranged by size:
1917 1772 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 2499 4240 5217 1203
Size: 1296 1741 977 1327
Fragments arranged by size:
1741 1327 1296 977
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 2579 1166
Size: 1413 3928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 3084 4656 3084
Size: 1572 3769
ApaI G_GGCC'C
Cuts at: 2517 2517
Size: 5341
ApaLI G'TGCA_C
Cuts at: 2312 3398 4554 2312
Size: 1086 1156 3099
Fragments arranged by size:
3099 1156 1086
ApoI r'AATT_y
Cuts at: 1493 2650 2876 3028 5137 1493
Size: 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2666 1870
Size: 73 723 4545
Fragments arranged by size:
4545 723 73
BamHI G'GATC_C
Cuts at: 2058 2585 2671 3038 2058
Size: 527 86 367 4361
Fragments arranged by size:
4361 527 367 86
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2662 4117 618
Size: 325 217 627 875 1455 1842
Fragments arranged by size:
1842 1455 875 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2517 2660 1391
Size: 1126 143 4072
Fragments arranged by size:
4072 1126 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2081 2115 3532 3566 1903
Size: 34 144 34 1417 34 3678
Fragments arranged by size:
3678 1417 144 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3233 4443 3233
Size: 1210 4131
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3952 136
Size: 107 122 71 631 605 2280 1525
Fragments arranged by size:
2280 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5336 5336
Size: 5341
BlpI GC'TnA_GC
Cuts at: 2521 2758 2521
Size: 237 5104
Bme1580I G_kGCm'C
Cuts at: 1790 2316 2517 3402 4558 1790
Size: 526 201 885 1156 2573
Fragments arranged by size:
2573 1156 885 526 201
BmgBI CAC'GTC
Cuts at: 1318 2317 2502 1318
Size: 999 185 4157
Fragments arranged by size:
4157 999 185
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3072 4030 442
Size: 416 53 83 399 24 1655 958 1753
Fragments arranged by size:
1753 1655 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 4001 1239
Size: 965 1797 2579
Fragments arranged by size:
2579 1797 965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3466 4331 4629 3466
Size: 865 298 4178
Fragments arranged by size:
4178 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4004 882
Size: 617 2505 2219
Fragments arranged by size:
2505 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2638 3132 493
Size: 711 1434 494 2702
Fragments arranged by size:
2702 1434 711 494
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3148 3530 4165 275
Size: 53 83 186 936 1615 382 635 1451
Fragments arranged by size:
1615 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2055 3774 4172 4446 4593 5004 2055
Size: 1719 398 274 147 411 2392
Fragments arranged by size:
2392 1719 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2301 2331 2384 2414 2555
Size: 30 165 30 898 30 53 30 141
Cuts at: 2555 2585 1178
Size: 30 3934
Fragments arranged by size:
3934 898 165 141 53 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2474 2561 1186
Size: 934 354 87 3966
Fragments arranged by size:
3966 934 354 87
BseYI C'CCAG_C
Cuts at: 2516 4544 5040 2516
Size: 2028 496 2817
Fragments arranged by size:
2817 2028 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4768
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3552 4580 1540
Size: 275 186 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2316 2434 3402 3487 4558 2316
Size: 118 968 85 1071 3099
Fragments arranged by size:
3099 1071 968 118 85
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
BsmBI CGTCTCn'nnnn_
Cuts at: 2323 2411 2323
Size: 88 5253
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2316 2434 2517 2660 3402 3487 4558
Size: 399 526 118 83 143 742 85 1071
Cuts at: 4558 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 526 399 143 118 85
83
BspEI T'CCGG_A
Cuts at: 2055 5004 2055
Size: 2949 2392
BspHI T'CATG_A
Cuts at: 3230 4960 3230
Size: 1730 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2386 2696 844
Size: 1542 310 3489
Fragments arranged by size:
3489 1542 310
BsrBI CCG'CTC
Cuts at: 1394 1823 3228 1394
Size: 429 1405 3507
Fragments arranged by size:
3507 1405 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3836 4010 65
Size: 1183 2588 174 1396
Fragments arranged by size:
2588 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2453 3985 4172 1700
Size: 110 643 1532 187 2869
Fragments arranged by size:
2869 1532 643 187 110
BsrGI T'GTAC_A
Cuts at: 96 2139 96
Size: 2043 3298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 3401 4413 3401
Size: 1012 4329
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5341
BstBI TT'CG_AA
Cuts at: 2007 4145 2007
Size: 2138 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstYI r'GATC_y
Cuts at: 1972 2058 2463 2585 2671 3038 3423 3440 4892
Size: 86 405 122 86 367 385 17 1452
Cuts at: 4892 4978 4990 5336 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 405 385 367 346 122 86
86 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4789
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2360 2857 3651 3671 2360
Size: 497 794 20 4030
Fragments arranged by size:
4030 794 497 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2788 3677
Size: 54 1425 50 275 18 294 664 889
Cuts at: 3677 5306 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 664 294 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2788 1537
Size: 275 976 4090
Fragments arranged by size:
4090 976 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3271 1353
Size: 1918 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3928 4302 4448 387
Size: 1064 138 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 3404 4001 4788 5056 1239
Size: 393 572 1200 597 787 268 1524
Fragments arranged by size:
1524 1200 787 597 572 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2513 2513
Size: 5341
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
EcoRV GAT'ATC
Cuts at: 2173 2173
Size: 5341
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 4488 1678
Size: 2810 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2201 2233 2301 2333 3982
Size: 32 135 32 629 32 68 32 1649
Cuts at: 3982 4014 4056 4088 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1649 629 135 68 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2497 2685 2931 669
Size: 988 281 559 188 246 3079
Fragments arranged by size:
3079 988 559 281 246 188
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5341
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4455 4639 1305
Size: 3150 184 2007
Fragments arranged by size:
3150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 10
Size: 54 1425 343 294 3225
Fragments arranged by size:
3225 1425 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2579 3299 3658 3817 518
Size: 648 1413 720 359 159 2042
Fragments arranged by size:
2042 1413 720 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2757 3434 4582 4827 1606
Size: 1151 677 1148 245 2120
Fragments arranged by size:
2120 1151 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4789
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2699 4244 1132
Size: 97 1470 1545 2229
Fragments arranged by size:
2229 1545 1470 97
PasI CC'CwG_GG
Cuts at: 1348 2204 1348
Size: 856 4485
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5341
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PspOMI G'GGCC_C
Cuts at: 2513 2513
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2300 2367 2693 830
Size: 1470 67 326 3478
Fragments arranged by size:
3478 1470 326 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 3589 5301 3589
Size: 1712 3629
SfcI C'TryA_G
Cuts at: 826 1045 2296 2363 2689 3824 4505 4696 826
Size: 219 1251 67 326 1135 681 191 1471
Fragments arranged by size:
1471 1251 1135 681 326 219 191 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 2453 1700
Size: 753 4588
SmaI CCC'GGG
Cuts at: 1872 2668 1872
Size: 796 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3445 4346 4608 820
Size: 197 926 1502 901 262 1553
Fragments arranged by size:
1553 1502 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2638 3132 493
Size: 2145 494 2702
Fragments arranged by size:
2702 2145 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 2604 1517
Size: 1087 4254
StyI C'CwwG_G
Cuts at: 513 1065 2066 2780 513
Size: 552 1001 714 3074
Fragments arranged by size:
3074 1001 714 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2392 3368 3385 3538 3723 4168 325
Size: 1255 812 976 17 153 185 445 1498
Fragments arranged by size:
1498 1255 976 812 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 3587 5299
Size: 274 80 33 51 1443 162 1448 1712
Cuts at: 5299 96
Size: 138
Fragments arranged by size:
1712 1448 1443 274 162 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2384 2416 5301 5333 2384
Size: 32 2885 32 2392
Fragments arranged by size:
2885 2392 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2051 1316
Size: 735 4606
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2666 1870
Size: 796 4545
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI