Restriction map for
pFN21ASDA1623
(Circular) (Six-base) MAPSORT of: pFN21ASDA1623.seq Check: 1280 from: 1 to: 8425
pFN21ASDA1623 8425 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2632 3642 2632
Size: 1010 7415
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6235 278
Size: 53 83 186 936 4699 2468
Fragments arranged by size:
4699 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2802 2906 5768 1937
Size: 865 104 2862 4594
Fragments arranged by size:
4594 2862 865 104
Acc65I G'GTAC_C
Cuts at: 5746 5746
Size: 8425
AclI AA'CG_TT
Cuts at: 1563 6552 6925 1563
Size: 4989 373 3063
Fragments arranged by size:
4989 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3884 6488 7872 8140 1632
Size: 2252 2604 1384 268 1917
Fragments arranged by size:
2604 2252 1917 1384 268
AfeI AGC'GCT
Cuts at: 3416 5361 3416
Size: 1945 6480
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8228
AflIII A'CryG_T
Cuts at: 1203 2333 2787 4251 7324 8301 1203
Size: 1130 454 1464 3073 977 1327
Fragments arranged by size:
3073 1464 1327 1130 977 454
AgeI A'CCGG_T
Cuts at: 4161 7256 4161
Size: 3095 5330
AhdI GACnn_n'nnGTC
Cuts at: 7154 7154
Size: 8425
AleI CACnn'nnGTG
Cuts at: 1166 2334 3953 1166
Size: 1168 1619 5638
Fragments arranged by size:
5638 1619 1168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4409 1184
Size: 3225 5200
AlwNI CAG_nnn'CTG
Cuts at: 2846 3172 5524 5584 5712 6168 7740 2846
Size: 326 2352 60 128 456 1572 3531
Fragments arranged by size:
3531 2352 1572 456 326 128 60
ApaI G_GGCC'C
Cuts at: 4095 4106 5484 5565 4095
Size: 11 1378 81 6955
Fragments arranged by size:
6955 1378 81 11
ApaLI G'TGCA_C
Cuts at: 4384 6482 7638 4384
Size: 2098 1156 5171
Fragments arranged by size:
5171 2098 1156
ApoI r'AATT_y
Cuts at: 1493 2545 4982 5734 5960 6112 8221 1493
Size: 1052 2437 752 226 152 2109 1697
Fragments arranged by size:
2437 2109 1697 1052 752 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6979 160
Size: 872 5947 1606
Fragments arranged by size:
5947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8425
AvaI C'yCGr_G
Cuts at: 1870 1943 2297 3549 4568 4624 5120 5202 5648
Size: 73 354 1252 1019 56 496 82 446
Cuts at: 5648 5750 1870
Size: 102 4545
Fragments arranged by size:
4545 1252 1019 496 446 354 102 82
73 56
BamHI G'GATC_C
Cuts at: 5641 5755 6122 5641
Size: 114 367 7944
Fragments arranged by size:
7944 367 114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2200 2435 3501 3579
Size: 325 217 627 255 158 235 1066 78
Cuts at: 3579 3654 3676 4933 5198 5375 5504 5746 7201
Size: 75 22 1257 265 177 129 242 1455
Cuts at: 7201 618
Size: 1842
Fragments arranged by size:
1842 1455 1257 1066 627 325 265 255
242 235 217 177 158 129 78 75
22
BanII G_rGCy'C
Cuts at: 1391 2304 3556 3858 3947 4021 4044 4095 4106
Size: 913 1252 302 89 74 23 51 11
Cuts at: 4106 4538 4826 5411 5484 5565 5744 1391
Size: 432 288 585 73 81 179 4072
Fragments arranged by size:
4072 1252 913 585 432 302 288 179
89 81 74 73 51 23 11
BbeI G_GCGC'C
Cuts at: 2046 3505 3583 3680 5379 2046
Size: 1459 78 97 1699 5092
Fragments arranged by size:
5092 1699 1459 97 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2201 2806 3127 928
Size: 971 302 605 321 6226
Fragments arranged by size:
6226 971 605 321 302
BbvCI CC'TCA_GC
Cuts at: 3659 5096 5313 3659
Size: 1437 217 6771
Fragments arranged by size:
6771 1437 217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2144 2178 6616 6650 1903
Size: 34 207 34 4438 34 3678
Fragments arranged by size:
4438 3678 207 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6317 7527 6317
Size: 1210 7215
BclI T'GATC_A
Cuts at: 1548 2848 3444 1548
Size: 1300 596 6529
Fragments arranged by size:
6529 1300 596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4906 7036 136
Size: 107 122 71 631 605 3234 2130 1525
Fragments arranged by size:
3234 2130 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8420 8420
Size: 8425
BlpI GC'TnA_GC
Cuts at: 2943 3179 3882 4532 5208 5842 2943
Size: 236 703 650 676 634 5526
Fragments arranged by size:
5526 703 676 650 634 236
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2438 4095 4106 4388 5484 5543 5565
Size: 264 384 1657 11 282 1096 59 22
Cuts at: 5565 6486 7642 1790
Size: 921 1156 2573
Fragments arranged by size:
2573 1657 1156 1096 921 384 282 264
59 22 11
BmgBI CAC'GTC
Cuts at: 1318 2055 2221 2790 1318
Size: 737 166 569 6953
Fragments arranged by size:
6953 737 569 166
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3001 5050 6156
Size: 416 53 83 399 24 1584 2049 1106
Cuts at: 6156 7114 442
Size: 958 1753
Fragments arranged by size:
2049 1753 1584 1106 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3275 1056
Size: 2219 6206
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2588 4213 4876 5365 2053
Size: 535 1625 663 489 5113
Fragments arranged by size:
5113 1625 663 535 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2612 3329 3446 3738 3953 3989 4244
Size: 842 531 717 117 292 215 36 255
Cuts at: 4244 4676 7085 1239
Size: 432 2409 2579
Fragments arranged by size:
2579 2409 842 717 531 432 292 255
215 117 36
Bpu10I CC'TnA_GC
Cuts at: 3374 3569 3659 5096 5313 5350 3374
Size: 195 90 1437 217 37 6449
Fragments arranged by size:
6449 1437 217 195 90 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3386 4574 5096 6550 7415 7713 3386
Size: 1188 522 1454 865 298 4098
Fragments arranged by size:
4098 1454 1188 865 522 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4077 5117 5645 7088 882
Size: 617 2578 1040 528 1443 2219
Fragments arranged by size:
2578 2219 1443 1040 617 528
BsaAI yAC'GTr
Cuts at: 493 1204 2364 2627 4606 5722 6216 493
Size: 711 1160 263 1979 1116 494 2702
Fragments arranged by size:
2702 1979 1160 1116 711 494 263
BsaBI GATnn'nnATC
Cuts at: 8419 8419
Size: 8425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2114 3502 3580
Size: 53 83 186 936 510 71 1388 78
Cuts at: 3580 3677 3901 4371 5376 5595 6232 6614 7249
Size: 97 224 470 1005 219 637 382 635
Cuts at: 7249 275
Size: 1451
Fragments arranged by size:
1451 1388 1005 936 637 635 510 470
382 224 219 186 97 83 78 71
53
BsaWI w'CCGG_w
Cuts at: 2117 4161 6858 7256 7530 7677 8088 2117
Size: 2044 2697 398 274 147 411 2454
Fragments arranged by size:
2697 2454 2044 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5029 5059 1178
Size: 30 165 30 3626 30 4544
Fragments arranged by size:
4544 3626 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 3008 3065 3698 3701 3704 3707 3752
Size: 1285 537 57 633 3 3 3 45
Cuts at: 3752 3782 3800 3803 3806 3815 3824 3833 3836
Size: 30 18 3 3 9 9 9 3
Cuts at: 3836 4010 4202 4577 4754 4757 4760 4820 4837
Size: 174 192 375 177 3 3 60 17
Cuts at: 4837 5299 5330 1186
Size: 462 31 4281
Fragments arranged by size:
4281 1285 633 537 462 375 192 177
174 60 57 45 31 30 18 17
9 9 9 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2395 2776 2806 3520 4203 4629 4701 5258 5577
Size: 381 30 714 683 426 72 557 319
Cuts at: 5577 5656 5675 5681 5705 7628 8124 2395
Size: 79 19 6 24 1923 496 2696
Fragments arranged by size:
2696 1923 714 683 557 496 426 381
319 79 72 30 24 19 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2717 4301 1163
Size: 573 981 1584 5287
Fragments arranged by size:
5287 1584 981 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2247 4166 5875 6636 7664 1540
Size: 275 186 246 1919 1709 761 1028 2301
Fragments arranged by size:
2301 1919 1709 1028 761 275 246 186
BsiHKAI G_wGCw'C
Cuts at: 2304 4302 4388 4569 4575 4770 6486 6571 7642
Size: 1998 86 181 6 195 1716 85 1071
Cuts at: 7642 2304
Size: 3087
Fragments arranged by size:
3087 1998 1716 1071 195 181 86 85
6
BsmI GAATG_Cn'
Cuts at: 2292 5936 6029 2292
Size: 3644 93 4688
Fragments arranged by size:
4688 3644 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3206 4144 5586 3206
Size: 938 1442 6045
Fragments arranged by size:
6045 1442 938
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2304 2438 3556 3858 3947 4021
Size: 399 264 250 134 1118 302 89 74
Cuts at: 4021 4044 4095 4106 4302 4388 4538 4569 4575
Size: 23 51 11 196 86 150 31 6
Cuts at: 4575 4770 4826 5411 5484 5543 5565 5744 6486
Size: 195 56 585 73 59 22 179 742
Cuts at: 6486 6571 7642 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1118 1071 742 585 399 302 264
250 196 195 179 150 134 89 86
85 74 73 59 56 51 31 23
22 11 6
BspEI T'CCGG_A
Cuts at: 2117 8088 2117
Size: 5971 2454
BspHI T'CATG_A
Cuts at: 2682 4947 6314 8044 2682
Size: 2265 1367 1730 3063
Fragments arranged by size:
3063 2265 1730 1367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2632 3642 5780 844
Size: 1788 1010 2138 3489
Fragments arranged by size:
3489 2138 1788 1010
BsrBI CCG'CTC
Cuts at: 1394 1823 3712 6312 1394
Size: 429 1889 2600 3507
Fragments arranged by size:
3507 2600 1889 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6920 7094 65
Size: 1183 5672 174 1396
Fragments arranged by size:
5672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3303 3498 4161 5293 5459 7069 7256
Size: 110 1493 195 663 1132 166 1610 187
Cuts at: 7256 1700
Size: 2869
Fragments arranged by size:
2869 1610 1493 1132 663 195 187 166
110
BsrGI T'GTAC_A
Cuts at: 96 2829 96
Size: 2733 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8425
BssSI C'ACGA_G
Cuts at: 2657 4688 6485 7497 2657
Size: 2031 1797 1012 3585
Fragments arranged by size:
3585 2031 1797 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2548 794
Size: 1754 6671
BstBI TT'CG_AA
Cuts at: 2007 7229 2007
Size: 5222 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8425
BstXI CCAn_nnnn'nTGG
Cuts at: 3955 3955
Size: 8425
BstYI r'GATC_y
Cuts at: 1972 4359 5641 5755 6122 6507 6524 7976 8062
Size: 2387 1282 114 367 385 17 1452 86
Cuts at: 8062 8074 8420 1972
Size: 12 346 1977
Fragments arranged by size:
2387 1977 1452 1282 385 367 346 114
86 17 12
BstZ17I GTA'TAC
Cuts at: 2803 2803
Size: 8425
Bsu36I CC'TnA_GG
Cuts at: 3219 3219
Size: 8425
BtgI C'CryG_G
Cuts at: 513 1065 2022 2440 2988 3511 4455 4894 5370
Size: 552 957 418 548 523 944 439 476
Cuts at: 5370 5553 513
Size: 183 3385
Fragments arranged by size:
3385 957 944 552 548 523 476 439
418 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4691 487
Size: 683 56 3465 4221
Fragments arranged by size:
4221 3465 683 56
BtsI GCAGTG_nn'
Cuts at: 2418 4881 4970 5269 5477 5941 6735 6755 2418
Size: 2463 89 299 208 464 794 20 4088
Fragments arranged by size:
4088 2463 794 464 299 208 89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8390
DraI TTT'AAA
Cuts at: 5729 5729
Size: 8425
DraIII CAC_nnn'GTG
Cuts at: 2581 5549 2581
Size: 2968 5457
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7432 809
Size: 507 6116 1802
Fragments arranged by size:
6116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 3432 3990
Size: 54 1425 50 275 18 195 1407 558
Cuts at: 3990 4047 4408 4429 4495 4608 4635 4647 4798
Size: 57 361 21 66 113 27 12 151
Cuts at: 4798 4891 5243 5526 5556 5872 6761 8390 8
Size: 93 352 283 30 316 889 1629 43
Fragments arranged by size:
1629 1425 1407 889 558 361 352 316
283 275 195 151 113 93 66 57
54 50 43 30 27 21 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 5872 1537
Size: 275 4060 4090
Fragments arranged by size:
4090 4060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2894 3089 3386 3801 4307 4526 5189 5334
Size: 1541 195 297 415 506 219 663 145
Cuts at: 5334 6355 1353
Size: 1021 3423
Fragments arranged by size:
3423 1541 1021 663 506 415 297 219
195 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2306 3626 4025 4049 4300 7012
Size: 1064 138 717 1320 399 24 251 2712
Cuts at: 7012 7386 7532 387
Size: 374 146 1280
Fragments arranged by size:
2712 1320 1280 1064 717 399 374 251
146 138 24
EcoICRI GAG'CTC
Cuts at: 2302 2302
Size: 8425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2612 3329 3446 3738 3884 3953
Size: 393 449 531 717 117 292 146 69
Cuts at: 3953 3989 4244 4676 6488 7085 7872 8140 1239
Size: 36 255 432 1812 597 787 268 1524
Fragments arranged by size:
1812 1524 787 717 597 531 449 432
393 292 268 255 146 117 69 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3218 3932 3992 5312 3218
Size: 714 60 1320 6331
Fragments arranged by size:
6331 1320 714 60
EcoO109I rG'GnC_Cy
Cuts at: 2082 3478 3838 3925 4091 4102 4923 5253 5305
Size: 1396 360 87 166 11 821 330 52
Cuts at: 5305 5481 5521 5562 2082
Size: 176 40 41 4945
Fragments arranged by size:
4945 1396 821 360 330 176 166 87
52 41 40 11
EcoRI G'AATT_C
Cuts at: 5734 6112 5734
Size: 378 8047
EcoRV GAT'ATC
Cuts at: 4621 4621
Size: 8425
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3892 3892
Size: 8425
FspI TGC'GCA
Cuts at: 2405 6931 2405
Size: 4526 3899
HaeII r_GCGC'y
Cuts at: 1678 2046 3318 3418 3505 3583 3680 3704 4331
Size: 368 1272 100 87 78 97 24 627
Cuts at: 4331 4519 4669 4696 4759 4918 5363 5379 5663
Size: 188 150 27 63 159 445 16 284
Cuts at: 5663 7572 1678
Size: 1909 2531
Fragments arranged by size:
2531 1909 1272 627 445 368 284 188
159 150 100 97 87 78 63 27
24 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5201 5233 7066 7098 7140
Size: 32 135 32 3629 32 1833 32 42
Cuts at: 7140 7172 1373
Size: 32 2626
Fragments arranged by size:
3629 2626 1833 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2907 3364 4345 4480 5769 6015
Size: 988 281 969 457 981 135 1289 246
Cuts at: 6015 669
Size: 3079
Fragments arranged by size:
3079 1289 988 981 969 457 281 246
135
HindIII A'AGCT_T
Cuts at: 748 2132 748
Size: 1384 7041
HpaI GTT'AAC
Cuts at: 6015 6015
Size: 8425
KasI G'GCGC_C
Cuts at: 2042 3501 3579 3676 5375 2042
Size: 1459 78 97 1699 5092
Fragments arranged by size:
5092 1699 1459 97 78
KpnI G_GTAC'C
Cuts at: 5750 5750
Size: 8425
MfeI C'AATT_G
Cuts at: 6024 6024
Size: 8425
MluI A'CGCG_T
Cuts at: 2333 8301 2333
Size: 5968 2457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4149 5244 7539 7723 1305
Size: 2844 1095 2295 184 2007
Fragments arranged by size:
2844 2295 2007 1095 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3434 4610 4649 4893 10
Size: 54 1425 343 1602 1176 39 244 3542
Fragments arranged by size:
3542 1602 1425 1176 343 244 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 2334 2956 3953 4604 6383 6742
Size: 648 1119 49 622 997 651 1779 359
Cuts at: 6742 6901 518
Size: 159 2042
Fragments arranged by size:
2042 1779 1119 997 651 648 622 359
159 49
MspA1I CmG'CkG
Cuts at: 1606 2442 2810 3091 3163 3513 3636 3663 4399
Size: 836 368 281 72 350 123 27 736
Cuts at: 4399 4510 5434 5531 5709 5841 6518 7666 7911
Size: 111 924 97 178 132 677 1148 245
Cuts at: 7911 1606
Size: 2120
Fragments arranged by size:
2120 1148 924 836 736 677 368 350
281 245 178 132 123 111 97 72
27
NaeI GCC'GGC
Cuts at: 5461 5461
Size: 8425
NarI GG'CG_CC
Cuts at: 2043 3502 3580 3677 5376 2043
Size: 1459 78 97 1699 5092
Fragments arranged by size:
5092 1699 1459 97 78
NcoI C'CATG_G
Cuts at: 513 1065 2022 4455 5553 513
Size: 552 957 2433 1098 3385
Fragments arranged by size:
3385 2433 1098 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8425
NgoMIV G'CCGG_C
Cuts at: 5459 5459
Size: 8425
NheI G'CTAG_C
Cuts at: 1052 3271 1052
Size: 2219 6206
NotI GC'GGCC_GC
Cuts at: 5872 5872
Size: 8425
NspI r_CATG'y
Cuts at: 1132 1229 5783 7328 1132
Size: 97 4554 1545 2229
Fragments arranged by size:
4554 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4919 5484 1348
Size: 3571 565 4289
Fragments arranged by size:
4289 3571 565
PciI A'CATG_T
Cuts at: 7324 7324
Size: 8425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4417 5524 5712 1108
Size: 3309 1107 188 3821
Fragments arranged by size:
3821 3309 1107 188
PfoI T'CCnGG_A
Cuts at: 2589 2610 3843 3930 4257 2589
Size: 21 1233 87 327 6757
Fragments arranged by size:
6757 1233 327 87 21
PmeI GTTT'AAAC
Cuts at: 5729 5729
Size: 8425
PmlI CAC'GTG
Cuts at: 2627 4606 2627
Size: 1979 6446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 6494 6526 8007 8039 4238
Size: 32 2224 32 1481 32 4624
Fragments arranged by size:
4624 2224 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3478 4923 5253 3478
Size: 1445 330 6650
Fragments arranged by size:
6650 1445 330
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8425
PsiI TTA'TAA
Cuts at: 5995 5995
Size: 8425
PspOMI G'GGCC_C
Cuts at: 4091 4102 5480 5561 4091
Size: 11 1378 81 6955
Fragments arranged by size:
6955 1378 81 11
PspXI vC'TCGA_Gb
Cuts at: 4568 4568
Size: 8425
PstI C_TGCA'G
Cuts at: 830 2107 2157 2544 3168 3486 4398 4515 4977
Size: 1277 50 387 624 318 912 117 462
Cuts at: 4977 5023 5777 830
Size: 46 754 3478
Fragments arranged by size:
3478 1277 912 754 624 462 387 318
117 50 46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8425
PvuII CAG'CTG
Cuts at: 2810 3091 3163 4510 2810
Size: 281 72 1347 6725
Fragments arranged by size:
6725 1347 281 72
SacI G_AGCT'C
Cuts at: 2304 2304
Size: 8425
SacII CC_GC'GG
Cuts at: 2443 3514 2443
Size: 1071 7354
SalI G'TCGA_C
Cuts at: 1936 2905 5767 1936
Size: 969 2862 4594
Fragments arranged by size:
4594 2862 969
SanDI GG'GwC_CC
Cuts at: 4923 5253 4923
Size: 330 8095
SapI GCTCTTCn'nnn_
Cuts at: 3089 4307 5189 3089
Size: 1218 882 6325
Fragments arranged by size:
6325 1218 882
SbfI CC_TGCA'GG
Cuts at: 2157 5777 2157
Size: 3620 4805
ScaI AGT'ACT
Cuts at: 6673 8385 6673
Size: 1712 6713
SexAI A'CCwGG_T
Cuts at: 2169 2169
Size: 8425
SfcI C'TryA_G
Cuts at: 826 1045 2103 2153 2205 2540 3164 3482 4394
Size: 219 1058 50 52 335 624 318 912
Cuts at: 4394 4511 4973 5019 5773 6908 7589 7780 826
Size: 117 462 46 754 1135 681 191 1471
Fragments arranged by size:
1471 1135 1058 912 754 681 624 462
335 318 219 191 117 52 50 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 4906 4906
Size: 8425
SfoI GGC'GCC
Cuts at: 2044 3503 3581 3678 5377 2044
Size: 1459 78 97 1699 5092
Fragments arranged by size:
5092 1699 1459 97 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8425
SmaI CCC'GGG
Cuts at: 1872 4626 5204 5752 1872
Size: 2754 578 548 4545
Fragments arranged by size:
4545 2754 578 548
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3401 4568 4589 5111 5648 6529
Size: 197 926 1458 1167 21 522 537 881
Cuts at: 6529 7430 7692 820
Size: 901 262 1553
Fragments arranged by size:
1553 1458 1167 926 901 881 537 522
262 197 21
SnaBI TAC'GTA
Cuts at: 493 5722 6216 493
Size: 5229 494 2702
Fragments arranged by size:
5229 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8425
SphI G_CATG'C
Cuts at: 1132 5783 1132
Size: 4651 3774
SspI AAT'ATT
Cuts at: 5 52 6349 5
Size: 47 6297 2081
Fragments arranged by size:
6297 2081 47
StuI AGG'CCT
Cuts at: 1517 4676 4859 1517
Size: 3159 183 5083
Fragments arranged by size:
5083 3159 183
StyI C'CwwG_G
Cuts at: 513 1065 2022 4455 4866 4872 5553 5864 513
Size: 552 957 2433 411 6 681 311 3074
Fragments arranged by size:
3074 2433 957 681 552 411 311 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 2683 4226 4448 4621 4951 5077
Size: 1255 681 422 1543 222 173 330 126
Cuts at: 5077 6452 6469 6622 6807 7252 325
Size: 1375 17 153 185 445 1498
Fragments arranged by size:
1543 1498 1375 1255 681 445 422 330
222 185 173 153 126 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2763 2829 6671
Size: 274 80 33 51 1443 786 66 3842
Cuts at: 6671 8383 96
Size: 1712 138
Fragments arranged by size:
3842 1712 1443 786 274 138 80 66
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5029 5061 5626 5658 8385 8417 5029
Size: 32 565 32 2727 32 5037
Fragments arranged by size:
5037 2727 565 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8425
XbaI T'CTAG_A
Cuts at: 2722 5761 2722
Size: 3039 5386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4555 5008 4555
Size: 453 7972
XhoI C'TCGA_G
Cuts at: 1943 4568 5648 1943
Size: 2625 1080 4720
Fragments arranged by size:
4720 2625 1080
XmaI C'CCGG_G
Cuts at: 1870 4624 5202 5750 1870
Size: 2754 578 548 4545
Fragments arranged by size:
4545 2754 578 548
XmnI GAAnn'nnTTC
Cuts at: 6554 6554
Size: 8425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6233 276
Size: 53 83 186 936 4699 2468
Fragments arranged by size:
4699 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI FseI FspAI
NruI NsiI PacI PsrI RsrII SrfI SwaI