Restriction map for
pFN21ASDA1614
(Circular) (Six-base) MAPSORT of: pFN21ASDA1614.seq Check: 622 from: 1 to: 8299
pFN21ASDA1614 8299 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4059 4059
Size: 8299
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5144 6109 278
Size: 53 83 186 936 3608 965 2468
Fragments arranged by size:
3608 2468 965 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5642 1937
Size: 3705 4594
Acc65I G'GTAC_C
Cuts at: 5620 5620
Size: 8299
AclI AA'CG_TT
Cuts at: 1563 2949 6426 6799 1563
Size: 1386 3477 373 3063
Fragments arranged by size:
3477 3063 1386 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4019 4134 4452 6362 7746 8014 1632
Size: 2387 115 318 1910 1384 268 1917
Fragments arranged by size:
2387 1917 1910 1384 318 268 115
AfeI AGC'GCT
Cuts at: 2846 5435 2846
Size: 2589 5710
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8102
AflIII A'CryG_T
Cuts at: 1203 4927 7198 8175 1203
Size: 3724 2271 977 1327
Fragments arranged by size:
3724 2271 1327 977
AgeI A'CCGG_T
Cuts at: 5256 7130 5256
Size: 1874 6425
AhdI GACnn_n'nnGTC
Cuts at: 3090 7028 3090
Size: 3938 4361
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3077 3476 1184
Size: 1893 399 6007
Fragments arranged by size:
6007 1893 399
AlwNI CAG_nnn'CTG
Cuts at: 2435 3448 4538 4584 4919 4955 6042 7614 2435
Size: 1013 1090 46 335 36 1087 1572 3120
Fragments arranged by size:
3120 1572 1090 1087 1013 335 46 36
ApaI G_GGCC'C
Cuts at: 2079 2261 2637 3356 3482 4739 5158 2079
Size: 182 376 719 126 1257 419 5220
Fragments arranged by size:
5220 1257 719 419 376 182 126
ApaLI G'TGCA_C
Cuts at: 6356 7512 6356
Size: 1156 7143
ApoI r'AATT_y
Cuts at: 1493 5608 5834 5986 8095 1493
Size: 4115 226 152 2109 1697
Fragments arranged by size:
4115 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6853 160
Size: 872 5821 1606
Fragments arranged by size:
5821 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8299
AvaI C'yCGr_G
Cuts at: 1870 1943 3049 3249 3984 4680 5624 1870
Size: 73 1106 200 735 696 944 4545
Fragments arranged by size:
4545 1106 944 735 696 200 73
AvrII C'CTAG_G
Cuts at: 4055 4055
Size: 8299
BamHI G'GATC_C
Cuts at: 3801 5629 5996 3801
Size: 1828 367 6104
Fragments arranged by size:
6104 1828 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3053 3598 3607 3627 3679
Size: 325 217 627 1266 545 9 20 52
Cuts at: 3679 4441 4632 5411 5481 5620 7075 618
Size: 762 191 779 70 139 1455 1842
Fragments arranged by size:
1842 1455 1266 779 762 627 545 325
217 191 139 70 52 20 9
BanII G_rGCy'C
Cuts at: 1391 2079 2261 2576 2637 2657 2966 3119 3356
Size: 688 182 315 61 20 309 153 237
Cuts at: 3356 3482 3526 3711 3755 3783 3977 4023 4698
Size: 126 44 185 44 28 194 46 675
Cuts at: 4698 4739 5158 5618 1391
Size: 41 419 460 4072
Fragments arranged by size:
4072 688 675 460 419 315 309 237
194 185 182 153 126 61 46 44
44 41 28 20
BbeI G_GCGC'C
Cuts at: 3683 4445 5485 3683
Size: 762 1040 6497
Fragments arranged by size:
6497 1040 762
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7328
BbvCI CC'TCA_GC
Cuts at: 2119 4011 5514 2119
Size: 1892 1503 4904
Fragments arranged by size:
4904 1892 1503
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 6490 6524 1903
Size: 34 1694 34 2825 34 3678
Fragments arranged by size:
3678 2825 1694 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4556 6191 7401 4556
Size: 1635 1210 5454
Fragments arranged by size:
5454 1635 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8299
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2470 3231 6910
Size: 107 122 71 631 605 798 761 3679
Cuts at: 6910 136
Size: 1525
Fragments arranged by size:
3679 1525 798 761 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2779 8294 2779
Size: 5515 2784
BlpI GC'TnA_GC
Cuts at: 3113 3449 4807 5240 5716 3113
Size: 336 1358 433 476 5696
Fragments arranged by size:
5696 1358 476 433 336
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2261 2637 3056 3157 3207 3300 3339
Size: 289 182 376 419 101 50 93 39
Cuts at: 3339 3356 3482 3836 4739 5158 5338 5450 6360
Size: 17 126 354 903 419 180 112 910
Cuts at: 6360 7516 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 910 903 419 419 376 354
289 182 180 126 112 101 93 50
39 17
BmgBI CAC'GTC
Cuts at: 1318 2552 5353 1318
Size: 1234 2801 4264
Fragments arranged by size:
4264 2801 1234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3052 4180 6030
Size: 416 53 83 399 24 1635 1128 1850
Cuts at: 6030 6988 442
Size: 958 1753
Fragments arranged by size:
1850 1753 1635 1128 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8299
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2267 3302 3865 2134
Size: 133 1035 563 6568
Fragments arranged by size:
6568 1035 563 133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2568 2869 2877 2885 3272 3298 3437
Size: 998 331 301 8 8 387 26 139
Cuts at: 3437 3686 3695 3961 4178 4787 4941 6959 1239
Size: 249 9 266 217 609 154 2018 2579
Fragments arranged by size:
2579 2018 998 609 387 331 301 266
249 217 154 139 26 9 8 8
Bpu10I CC'TnA_GC
Cuts at: 2119 2142 3749 4011 4514 5514 5537 2119
Size: 23 1607 262 503 1000 23 4881
Fragments arranged by size:
4881 1607 1000 503 262 23 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6424 7289 7587 6424
Size: 865 298 7136
Fragments arranged by size:
7136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2853 6962 882
Size: 617 1354 4109 2219
Fragments arranged by size:
4109 2219 1354 617
BsaAI yAC'GTr
Cuts at: 493 1204 3370 5596 6090 493
Size: 711 2166 2226 494 2702
Fragments arranged by size:
2702 2226 2166 711 494
BsaBI GATnn'nnATC
Cuts at: 8293 8293
Size: 8299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3680 4283 4442 5141
Size: 53 83 186 936 2147 603 159 699
Cuts at: 5141 5482 6106 6488 7123 275
Size: 341 624 382 635 1451
Fragments arranged by size:
2147 1451 936 699 635 624 603 382
341 186 159 83 53
BsaWI w'CCGG_w
Cuts at: 4753 5256 6732 7130 7404 7551 7962 4753
Size: 503 1476 398 274 147 411 5090
Fragments arranged by size:
5090 1476 503 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3750 3780 3813 3843 4856
Size: 30 165 30 2347 30 33 30 1013
Cuts at: 4856 4886 4914 4944 1178
Size: 30 28 30 4533
Fragments arranged by size:
4533 2347 1013 165 33 30 30 30
30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2436 2510 2822 3637 3914 4877 5496 1186
Size: 1250 74 312 815 277 963 619 3989
Fragments arranged by size:
3989 1250 963 815 619 312 277 74
BseYI C'CCAG_C
Cuts at: 2223 2629 2636 2723 2836 2879 3244 3739 3924
Size: 406 7 87 113 43 365 495 185
Cuts at: 3924 4041 4207 4437 4519 4608 4613 5083 5363
Size: 117 166 230 82 89 5 470 280
Cuts at: 5363 5526 7502 7998 2223
Size: 163 1976 496 2524
Fragments arranged by size:
2524 1976 496 495 470 406 365 280
230 185 166 163 117 113 89 87
82 43 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2997 5159 5319 1163
Size: 573 1261 2162 160 4143
Fragments arranged by size:
4143 2162 1261 573 160
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5749 6510 7538 1540
Size: 275 186 3748 761 1028 2301
Fragments arranged by size:
3748 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3455 3711 3755 4023 5362 6360 6445 7516 3455
Size: 256 44 268 1339 998 85 1071 4238
Fragments arranged by size:
4238 1339 1071 998 268 256 85 44
BsmI GAATG_Cn'
Cuts at: 5810 5903 5810
Size: 93 8206
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2261 2576 2637 2657 2966 3056
Size: 399 289 182 315 61 20 309 90
Cuts at: 3056 3119 3157 3207 3300 3339 3356 3455 3482
Size: 63 38 50 93 39 17 99 27
Cuts at: 3482 3526 3711 3755 3783 3836 3977 4023 4698
Size: 44 185 44 28 53 141 46 675
Cuts at: 4698 4739 5158 5338 5362 5450 5618 6360 6445
Size: 41 419 180 24 88 168 742 85
Cuts at: 6445 7516 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 675 419 399 315 309
289 185 182 180 168 141 99 93
90 88 85 63 61 53 50 46
44 44 41 39 38 28 27 24
20 17
BspEI T'CCGG_A
Cuts at: 7962 7962
Size: 8299
BspHI T'CATG_A
Cuts at: 6188 7918 6188
Size: 1730 6569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2469 4059 5654 844
Size: 1625 1590 1595 3489
Fragments arranged by size:
3489 1625 1595 1590
BsrBI CCG'CTC
Cuts at: 1394 1823 3421 6186 1394
Size: 429 1598 2765 3507
Fragments arranged by size:
3507 2765 1598 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6794 6968 65
Size: 1183 5546 174 1396
Fragments arranged by size:
5546 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3604 5256 5311 6943 7130 1700
Size: 110 1794 1652 55 1632 187 2869
Fragments arranged by size:
2869 1794 1652 1632 187 110 55
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8299
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8299
BssSI C'ACGA_G
Cuts at: 3301 4331 4715 6359 7371 3301
Size: 1030 384 1644 1012 4229
Fragments arranged by size:
4229 1644 1030 1012 384
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2941 3145 4051 4639 4817 794
Size: 2147 204 906 588 178 4276
Fragments arranged by size:
4276 2147 906 588 204 178
BstBI TT'CG_AA
Cuts at: 2007 7103 2007
Size: 5096 3203
BstEII G'GTnAC_C
Cuts at: 1184 4523 1184
Size: 3339 4960
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 2178 2215 2107
Size: 71 37 8191
Fragments arranged by size:
8191 71 37
BstYI r'GATC_y
Cuts at: 1972 2779 3801 4916 5629 5996 6381 6398 7850
Size: 807 1022 1115 713 367 385 17 1452
Cuts at: 7850 7936 7948 8294 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1115 1022 807 713 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2190 4623 2190
Size: 2433 5866
BtgI C'CryG_G
Cuts at: 513 1065 2022 2055 2106 2576 2662 3468 4833
Size: 552 957 33 51 470 86 806 1365
Cuts at: 4833 5327 5379 5521 513
Size: 494 52 142 3291
Fragments arranged by size:
3291 1365 957 806 552 494 470 142
86 52 51 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7560
Fragments arranged by size:
7560 683 56
BtsI GCAGTG_nn'
Cuts at: 2893 4637 5815 6609 6629 2893
Size: 1744 1178 794 20 4563
Fragments arranged by size:
4563 1744 1178 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8264
DraI TTT'AAA
Cuts at: 5603 5603
Size: 8299
DraIII CAC_nnn'GTG
Cuts at: 3831 5182 5333 3831
Size: 1351 151 6797
Fragments arranged by size:
6797 1351 151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2740 5146 7306 809
Size: 507 1424 2406 2160 1802
Fragments arranged by size:
2406 2160 1802 1424 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2310 3073
Size: 54 1425 50 275 18 216 264 763
Cuts at: 3073 3232 5313 5746 6635 8264 8
Size: 159 2081 433 889 1629 43
Fragments arranged by size:
2081 1629 1425 889 763 433 275 264
216 159 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5746 1537
Size: 275 3934 4090
Fragments arranged by size:
4090 3934 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5054 5514 6229 1353
Size: 3701 460 715 3423
Fragments arranged by size:
3701 3423 715 460
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3379 3952 4031 6886 7260 7406
Size: 1064 138 1790 573 79 2855 374 146
Cuts at: 7406 387
Size: 1280
Fragments arranged by size:
2855 1790 1280 1064 573 374 146 138
79
EcoICRI GAG'CTC
Cuts at: 3709 3753 4021 3709
Size: 44 268 7987
Fragments arranged by size:
7987 268 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2568 2869 2877 2885 3272 3298
Size: 393 605 331 301 8 8 387 26
Cuts at: 3298 3437 3686 3695 3961 4019 4134 4178 4452
Size: 139 249 9 266 58 115 44 274
Cuts at: 4452 4787 4941 6362 6959 7746 8014 1239
Size: 335 154 1421 597 787 268 1524
Fragments arranged by size:
1524 1421 787 605 597 393 387 335
331 301 274 268 266 249 154 139
115 58 44 26 9 8 8
EcoNI CCTnn'n_nnAGG
Cuts at: 4200 5393 4200
Size: 1193 7106
EcoO109I rG'GnC_Cy
Cuts at: 2075 2115 2257 2258 2519 2633 2690 2742 3171
Size: 40 142 1 261 114 57 52 429
Cuts at: 3171 3253 3352 3353 3478 3479 3696 3909 4030
Size: 82 99 1 125 1 217 213 121
Cuts at: 4030 4181 4581 4735 4736 4787 4964 5154 5155
Size: 151 400 154 1 51 177 190 1
Cuts at: 5155 5553 2075
Size: 398 4821
Fragments arranged by size:
4821 429 400 398 261 217 213 190
177 154 151 142 125 121 114 99
82 57 52 51 40 1 1 1
1 1
EcoRI G'AATT_C
Cuts at: 5608 5986 5608
Size: 378 7921
FseI GG_CCGG'CC
Cuts at: 5315 5315
Size: 8299
FspI TGC'GCA
Cuts at: 6805 6805
Size: 8299
HaeII r_GCGC'y
Cuts at: 1678 2848 2880 3436 3442 3683 4445 5437 5485
Size: 1170 32 556 6 241 762 992 48
Cuts at: 5485 7446 1678
Size: 1961 2531
Fragments arranged by size:
2531 1961 1170 992 762 556 241 48
32 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2339 2371 2777 2809 3032
Size: 32 135 32 767 32 406 32 223
Cuts at: 3032 3064 3076 3108 3371 3403 4856 4888 6940
Size: 32 12 32 263 32 1453 32 2052
Cuts at: 6940 6972 7014 7046 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2052 1453 767 406 263 223 135
42 32 32 32 32 32 32 32
32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 5643 5889 669
Size: 988 281 3705 246 3079
Fragments arranged by size:
3705 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8299
HpaI GTT'AAC
Cuts at: 5889 5889
Size: 8299
KasI G'GCGC_C
Cuts at: 3679 4441 5481 3679
Size: 762 1040 6497
Fragments arranged by size:
6497 1040 762
KpnI G_GTAC'C
Cuts at: 5624 5624
Size: 8299
MfeI C'AATT_G
Cuts at: 5898 5898
Size: 8299
MluI A'CGCG_T
Cuts at: 8175 8175
Size: 8299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5183 7413 7597 1305
Size: 3878 2230 184 2007
Fragments arranged by size:
3878 2230 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2312 3234 10
Size: 54 1425 343 480 922 5075
Fragments arranged by size:
5075 1425 922 480 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6257 6616 6775 518
Size: 648 5091 359 159 2042
Fragments arranged by size:
5091 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2057 2123 2152 2476 2626 3011 3318 3330
Size: 451 66 29 324 150 385 307 12
Cuts at: 3330 3470 3645 5303 5422 5428 5715 6392 7540
Size: 140 175 1658 119 6 287 677 1148
Cuts at: 7540 7785 1606
Size: 245 2120
Fragments arranged by size:
2120 1658 1148 677 451 385 324 307
287 245 175 150 140 119 66 29
12 6
NaeI GCC'GGC
Cuts at: 3606 5313 3606
Size: 1707 6592
NarI GG'CG_CC
Cuts at: 3680 4442 5482 3680
Size: 762 1040 6497
Fragments arranged by size:
6497 1040 762
NcoI C'CATG_G
Cuts at: 513 1065 2022 2662 5521 513
Size: 552 957 640 2859 3291
Fragments arranged by size:
3291 2859 957 640 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8299
NgoMIV G'CCGG_C
Cuts at: 3604 5311 3604
Size: 1707 6592
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8299
NotI GC'GGCC_GC
Cuts at: 5746 5746
Size: 8299
NspI r_CATG'y
Cuts at: 1132 1229 4931 5657 7202 1132
Size: 97 3702 726 1545 2229
Fragments arranged by size:
3702 2229 1545 726 97
PasI CC'CwG_GG
Cuts at: 1348 2232 3217 3670 4177 4792 5032 5059 5441
Size: 884 985 453 507 615 240 27 382
Cuts at: 5441 1348
Size: 4206
Fragments arranged by size:
4206 985 884 615 507 453 382 240
27
PciI A'CATG_T
Cuts at: 4927 7198 4927
Size: 2271 6028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2238 1108
Size: 1130 7169
PfoI T'CCnGG_A
Cuts at: 2674 3534 3702 3804 4238 2674
Size: 860 168 102 434 6735
Fragments arranged by size:
6735 860 434 168 102
PmeI GTTT'AAAC
Cuts at: 5603 5603
Size: 8299
PmlI CAC'GTG
Cuts at: 3370 3370
Size: 8299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6368 6400 7881 7913 6368
Size: 32 1481 32 6754
Fragments arranged by size:
6754 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2115 2690 2742 3171 3253 3696 3909 4181 4581
Size: 575 52 429 82 443 213 272 400
Cuts at: 4581 4787 4964 2115
Size: 206 177 5450
Fragments arranged by size:
5450 575 443 429 400 272 213 206
177 82 52
PshAI GACnn'nnGTC
Cuts at: 1613 2619 1613
Size: 1006 7293
PsiI TTA'TAA
Cuts at: 5869 5869
Size: 8299
PspOMI G'GGCC_C
Cuts at: 2075 2257 2633 3352 3478 4735 5154 2075
Size: 182 376 719 126 1257 419 5220
Fragments arranged by size:
5220 1257 719 419 376 182 126
PspXI vC'TCGA_Gb
Cuts at: 3984 3984
Size: 8299
PstI C_TGCA'G
Cuts at: 830 2937 3150 3278 3429 3778 3887 4692 4745
Size: 2107 213 128 151 349 109 805 53
Cuts at: 4745 4902 5427 5433 5651 830
Size: 157 525 6 218 3478
Fragments arranged by size:
3478 2107 805 525 349 218 213 157
151 128 109 53 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8299
PvuII CAG'CTG
Cuts at: 2123 2152 2476 3011 3645 5303 5422 5428 2123
Size: 29 324 535 634 1658 119 6 4994
Fragments arranged by size:
4994 1658 634 535 324 119 29 6
SacI G_AGCT'C
Cuts at: 3711 3755 4023 3711
Size: 44 268 7987
Fragments arranged by size:
7987 268 44
SacII CC_GC'GG
Cuts at: 2058 3471 2058
Size: 1413 6886
SalI G'TCGA_C
Cuts at: 1936 5641 1936
Size: 3705 4594
SanDI GG'GwC_CC
Cuts at: 3253 4181 3253
Size: 928 7371
SapI GCTCTTCn'nnn_
Cuts at: 5054 5054
Size: 8299
SbfI CC_TGCA'GG
Cuts at: 5651 5651
Size: 8299
ScaI AGT'ACT
Cuts at: 2558 6547 8259 2558
Size: 3989 1712 2598
Fragments arranged by size:
3989 2598 1712
SexAI A'CCwGG_T
Cuts at: 5271 5271
Size: 8299
SfcI C'TryA_G
Cuts at: 826 1045 2933 3146 3274 3425 3774 3883 4688
Size: 219 1888 213 128 151 349 109 805
Cuts at: 4688 4741 4898 5101 5423 5429 5647 6782 7463
Size: 53 157 203 322 6 218 1135 681
Cuts at: 7463 7654 826
Size: 191 1471
Fragments arranged by size:
1888 1471 1135 805 681 349 322 219
218 213 203 191 157 151 128 109
53 6
SfoI GGC'GCC
Cuts at: 3681 4443 5483 3681
Size: 762 1040 6497
Fragments arranged by size:
6497 1040 762
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8299
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8299
SmaI CCC'GGG
Cuts at: 1872 3251 5626 1872
Size: 1379 2375 4545
Fragments arranged by size:
4545 2375 1379
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3984 6403 7304 7566 820
Size: 197 926 2041 2419 901 262 1553
Fragments arranged by size:
2419 2041 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5596 6090 493
Size: 5103 494 2702
Fragments arranged by size:
5103 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8299
SphI G_CATG'C
Cuts at: 1132 5657 1132
Size: 4525 3774
SspI AAT'ATT
Cuts at: 5 52 6223 5
Size: 47 6171 2081
Fragments arranged by size:
6171 2081 47
StuI AGG'CCT
Cuts at: 1517 3635 3982 1517
Size: 2118 347 5834
Fragments arranged by size:
5834 2118 347
StyI C'CwwG_G
Cuts at: 513 1065 2022 2071 2277 2662 2729 3943 4025
Size: 552 957 49 206 385 67 1214 82
Cuts at: 4025 4055 5521 5738 513
Size: 30 1466 217 3074
Fragments arranged by size:
3074 1466 1214 957 552 385 217 206
82 67 49 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2828 3731 3905 4511 4650 5345 6326
Size: 1255 1248 903 174 606 139 695 981
Cuts at: 6326 6343 6496 6681 7126 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1248 981 903 695 606 445
185 174 153 139 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2245 2556 6545
Size: 274 80 33 51 1443 268 311 3989
Cuts at: 6545 8257 96
Size: 1712 138
Fragments arranged by size:
3989 1712 1443 311 274 268 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3750 3782 3813 3845 3880 3912 3983 4015 4914
Size: 32 31 32 35 32 71 32 899
Cuts at: 4914 4946 8259 8291 3750
Size: 32 3313 32 3758
Fragments arranged by size:
3758 3313 899 71 35 32 32 32
32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 4909 1316
Size: 3593 4706
XbaI T'CTAG_A
Cuts at: 5635 5635
Size: 8299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2592 3719 4193 4401 4799 5269 5300 2592
Size: 1127 474 208 398 470 31 5591
Fragments arranged by size:
5591 1127 474 470 398 208 31
XhoI C'TCGA_G
Cuts at: 1943 3984 1943
Size: 2041 6258
XmaI C'CCGG_G
Cuts at: 1870 3249 5624 1870
Size: 1379 2375 4545
Fragments arranged by size:
4545 2375 1379
XmnI GAAnn'nnTTC
Cuts at: 6428 6428
Size: 8299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5142 6107 276
Size: 53 83 186 936 3608 965 2468
Fragments arranged by size:
3608 2468 965 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV
FalI FspAI NruI NsiI PacI PsrI RsrII SfiI
SrfI SwaI