Restriction map for
pFN21ASDA1609
(Circular) (Six-base) MAPSORT of: pFN21ASDA1609.seq Check: 4387 from: 1 to: 6097
pFN21ASDA1609 6097 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2500 2500
Size: 6097
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2241 3440 1937
Size: 304 1199 4594
Fragments arranged by size:
4594 1199 304
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 4160 5544 5812 1632
Size: 551 1977 1384 268 1917
Fragments arranged by size:
1977 1917 1384 551 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 2277 3057 4996 5973 1203
Size: 1074 780 1939 977 1327
Fragments arranged by size:
1939 1327 1074 977 780
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6097
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2044 2076 2606 2638 2044
Size: 32 530 32 5503
Fragments arranged by size:
5503 530 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2461 2515 2581 3328 3840 5412 2461
Size: 54 66 747 512 1572 3146
Fragments arranged by size:
3146 1572 747 512 66 54
ApaI G_GGCC'C
Cuts at: 2496 2574 2895 2496
Size: 78 321 5698
Fragments arranged by size:
5698 321 78
ApaLI G'TGCA_C
Cuts at: 2435 3181 4154 5310 2435
Size: 746 973 1156 3222
Fragments arranged by size:
3222 1156 973 746
ApoI r'AATT_y
Cuts at: 1493 3406 3632 3784 5893 1493
Size: 1913 226 152 2109 1697
Fragments arranged by size:
2109 1913 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2502 2799 2909 3422 1870
Size: 73 559 297 110 513 4545
Fragments arranged by size:
4545 559 513 297 110 73
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2811 2820 3418 4873 618
Size: 325 217 627 1024 9 598 1455 1842
Fragments arranged by size:
1842 1455 1024 627 598 325 217 9
BanII G_rGCy'C
Cuts at: 1391 2496 2574 2844 2895 3369 3416 1391
Size: 1105 78 270 51 474 47 4072
Fragments arranged by size:
4072 1105 474 270 78 51 47
BbeI G_GCGC'C
Cuts at: 2824 2824
Size: 6097
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 2095 1548
Size: 547 5550
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2578 4708 136
Size: 107 122 71 631 605 906 2130 1525
Fragments arranged by size:
2130 1525 906 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 2444 2523 3514 2444
Size: 79 991 5027
Fragments arranged by size:
5027 991 79
Bme1580I G_kGCm'C
Cuts at: 1790 2439 2496 2574 2895 3185 4158 5314 1790
Size: 649 57 78 321 290 973 1156 2573
Fragments arranged by size:
2573 1156 973 649 321 290 78 57
BmgBI CAC'GTC
Cuts at: 1318 2182 1318
Size: 864 5233
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2262 2484 2569
Size: 416 53 83 399 24 845 222 85
Cuts at: 2569 2626 3107 3828 4786 442
Size: 57 481 721 958 1753
Fragments arranged by size:
1753 958 845 721 481 416 399 222
85 83 57 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3353 4757 1239
Size: 2114 1404 2579
Fragments arranged by size:
2579 2114 1404
Bpu10I CC'TnA_GC
Cuts at: 2718 3213 2718
Size: 495 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3252 4760 882
Size: 617 1753 1508 2219
Fragments arranged by size:
2219 1753 1508 617
BsaAI yAC'GTr
Cuts at: 493 1204 2440 3180 3189 3394 3888 493
Size: 711 1236 740 9 205 494 2702
Fragments arranged by size:
2702 1236 740 711 494 205 9
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2821 3904 4286 4921
Size: 53 83 186 936 1288 1083 382 635
Cuts at: 4921 275
Size: 1451
Fragments arranged by size:
1451 1288 1083 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4530 4928 5202 5349 5760 4530
Size: 398 274 147 411 4867
Fragments arranged by size:
4867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2044 2074 2843 2873 1178
Size: 30 165 30 641 30 769 30 4402
Fragments arranged by size:
4402 769 641 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 3284 1186
Size: 1162 936 3999
Fragments arranged by size:
3999 1162 936
BseYI C'CCAG_C
Cuts at: 2790 2862 3014 5300 5796 2790
Size: 72 152 2286 496 3091
Fragments arranged by size:
3091 2286 496 152 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 1163
Size: 573 792 4732
Fragments arranged by size:
4732 792 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3547 4308 5336 1540
Size: 275 186 1546 761 1028 2301
Fragments arranged by size:
2301 1546 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2439 2690 2844 3185 4158 4243 5314 2439
Size: 251 154 341 973 85 1071 3222
Fragments arranged by size:
3222 1071 973 341 251 154 85
BsmI GAATG_Cn'
Cuts at: 3346 3608 3701 3346
Size: 262 93 5742
Fragments arranged by size:
5742 262 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2697 2697
Size: 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 2496 2574 2690 2844 2895 3185
Size: 399 649 57 78 116 154 51 290
Cuts at: 3185 3369 3416 4158 4243 5314 1391
Size: 184 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 649 399 290 184 154
116 85 78 57 51 47
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 2346 3986 5716 2346
Size: 1640 1730 2727
Fragments arranged by size:
2727 1730 1640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2500 3452 844
Size: 1656 952 3489
Fragments arranged by size:
3489 1656 952
BsrBI CCG'CTC
Cuts at: 1394 1823 3984 1394
Size: 429 2161 3507
Fragments arranged by size:
3507 2161 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4592 4766 65
Size: 1183 3344 174 1396
Fragments arranged by size:
3344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4741 4928 1700
Size: 110 2931 187 2869
Fragments arranged by size:
2931 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2778 3027 96
Size: 2682 249 3166
Fragments arranged by size:
3166 2682 249
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 2007 4901 2007
Size: 2894 3203
BstEII G'GTnAC_C
Cuts at: 1184 2209 1184
Size: 1025 5072
BstXI CCAn_nnnn'nTGG
Cuts at: 2870 2870
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 2416 3427 3794 4179 4196 5648 5734 5746
Size: 444 1011 367 385 17 1452 86 12
Cuts at: 5746 6092 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1011 444 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2076 3315 2076
Size: 1239 4858
BtgI C'CryG_G
Cuts at: 513 1065 2022 2845 3070 513
Size: 552 957 823 225 3540
Fragments arranged by size:
3540 957 823 552 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 3613 4407 4427 3613
Size: 794 20 5283
Fragments arranged by size:
5283 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2640 2872 3279
Size: 54 1425 50 275 18 810 232 407
Cuts at: 3279 3544 4433 6062 8
Size: 265 889 1629 43
Fragments arranged by size:
1629 1425 889 810 407 275 265 232
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3544 1537
Size: 275 1732 4090
Fragments arranged by size:
4090 1732 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2333 4027 1353
Size: 846 134 1694 3423
Fragments arranged by size:
3423 1694 846 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2065 4684 5058 5204 387
Size: 1064 138 476 2619 374 146 1280
Fragments arranged by size:
2619 1280 1064 476 374 146 138
EcoICRI GAG'CTC
Cuts at: 2842 2842
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 3353 4160 4757 5544 5812 1239
Size: 393 551 1170 807 597 787 268 1524
Fragments arranged by size:
1524 1170 807 787 597 551 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2264 2378 2492 2570 2571 2629 2891 2892 3325
Size: 114 114 78 1 58 262 1 433
Cuts at: 3325 2264
Size: 5036
Fragments arranged by size:
5036 433 262 114 114 78 58 1
1
EcoRI G'AATT_C
Cuts at: 3406 3784 3406
Size: 378 5719
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 2824 5244 1678
Size: 1146 2420 2531
Fragments arranged by size:
2531 2420 1146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 2264 2296 2592
Size: 32 135 32 527 32 133 32 296
Cuts at: 2592 2624 2753 2785 4738 4770 4812 4844 1373
Size: 32 129 32 1953 32 42 32 2626
Fragments arranged by size:
2626 1953 527 296 135 133 129 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2242 3441 3687 669
Size: 988 281 304 1199 246 3079
Fragments arranged by size:
3079 1199 988 304 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KasI G'GCGC_C
Cuts at: 2820 2820
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 2099 3696 2099
Size: 1597 4500
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3235 5211 5395 1305
Size: 1930 1976 184 2007
Fragments arranged by size:
2007 1976 1930 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2642 2874 3281 10
Size: 54 1425 343 810 232 407 2826
Fragments arranged by size:
2826 1425 810 407 343 232 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 3023 4055 4414 4573 518
Size: 648 1185 672 1032 359 159 2042
Fragments arranged by size:
2042 1185 1032 672 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2527 2794 2809 2817 3205 3513 4190 5338
Size: 921 267 15 8 388 308 677 1148
Cuts at: 5338 5583 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 921 677 388 308 267 245
15 8
NarI GG'CG_CC
Cuts at: 2821 2821
Size: 6097
NcoI C'CATG_G
Cuts at: 513 1065 2022 2845 3070 513
Size: 552 957 823 225 3540
Fragments arranged by size:
3540 957 823 552 225
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 2233 2930 3061 3455 5000 1132
Size: 97 1004 697 131 394 1545 2229
Fragments arranged by size:
2229 1545 1004 697 394 131 97
PasI CC'CwG_GG
Cuts at: 1348 2489 1348
Size: 1141 4956
PciI A'CATG_T
Cuts at: 3057 4996 3057
Size: 1939 4158
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2581 1108
Size: 1473 4624
PfoI T'CCnGG_A
Cuts at: 2757 3300 3369 3378 2757
Size: 543 69 9 5476
Fragments arranged by size:
5476 543 69 9
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PmlI CAC'GTG
Cuts at: 2440 3180 2440
Size: 740 5357
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2076 4166 4198 5679 5711 2044
Size: 32 2090 32 1481 32 2430
Fragments arranged by size:
2430 2090 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2264 2378 2629 2264
Size: 114 251 5732
Fragments arranged by size:
5732 251 114
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 2492 2570 2891 2492
Size: 78 321 5698
Fragments arranged by size:
5698 321 78
PspXI vC'TCGA_Gb
Cuts at: 2909 2909
Size: 6097
PstI C_TGCA'G
Cuts at: 830 3449 830
Size: 2619 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 2527 2794 3205 2527
Size: 267 411 5419
Fragments arranged by size:
5419 411 267
SacI G_AGCT'C
Cuts at: 2844 2844
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 2240 3439 1936
Size: 304 1199 4594
Fragments arranged by size:
4594 1199 304
SanDI GG'GwC_CC
Cuts at: 2264 2629 2264
Size: 365 5732
SapI GCTCTTCn'nnn_
Cuts at: 2199 2199
Size: 6097
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 2171 3445 4580 5261 5452 826
Size: 219 1126 1274 1135 681 191 1471
Fragments arranged by size:
1471 1274 1135 1126 681 219 191
SfoI GGC'GCC
Cuts at: 2822 2822
Size: 6097
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 2504 3424 1872
Size: 632 920 4545
Fragments arranged by size:
4545 920 632
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2909 4201 5102 5364 820
Size: 197 926 966 1292 901 262 1553
Fragments arranged by size:
1553 1292 966 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3394 3888 493
Size: 2901 494 2702
Fragments arranged by size:
2901 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 2233 3455 1132
Size: 1101 1222 3774
Fragments arranged by size:
3774 1222 1101
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2022 2845 3070 3282 3536 513
Size: 552 957 823 225 212 254 3074
Fragments arranged by size:
3074 957 823 552 254 225 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2608 2613 3124 4124 4141 4294 4479
Size: 1255 1028 5 511 1000 17 153 185
Cuts at: 4479 4924 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1028 1000 511 445 185 153
17 5
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2778 3027 3060
Size: 274 80 33 51 1443 801 249 33
Cuts at: 3060 4343 6055 96
Size: 1283 1712 138
Fragments arranged by size:
1712 1443 1283 801 274 249 138 80
51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2362 2394 2843 2875 6057 6089 2362
Size: 32 449 32 3182 32 2370
Fragments arranged by size:
3182 2370 449 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 2909 1943
Size: 966 5131
XmaI C'CCGG_G
Cuts at: 1870 2502 3422 1870
Size: 632 920 4545
Fragments arranged by size:
4545 920 632
XmnI GAAnn'nnTTC
Cuts at: 2193 4226 2193
Size: 2033 4064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PsrI RsrII SacII SexAI
SfiI SrfI SwaI XcmI