Restriction map for
pFN21ASDA1605
(Circular) (Six-base) MAPSORT of: pFN21ASDA1605.seq Check: 8646 from: 1 to: 7360
pFN21ASDA1605 7360 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2777 3094 3355 2777
Size: 317 261 6782
Fragments arranged by size:
6782 317 261
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5170 278
Size: 53 83 186 936 3634 2468
Fragments arranged by size:
3634 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3311 4497 4703 1937
Size: 1374 1186 206 4594
Fragments arranged by size:
4594 1374 1186 206
Acc65I G'GTAC_C
Cuts at: 2382 2397 3747 4681 2382
Size: 15 1350 934 5061
Fragments arranged by size:
5061 1350 934 15
AclI AA'CG_TT
Cuts at: 1563 5487 5860 1563
Size: 3924 373 3063
Fragments arranged by size:
3924 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2136 3107 3908 3965 4322 4521 4622 5423
Size: 504 971 801 57 357 199 101 801
Cuts at: 5423 6807 7075 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 971 801 801 504 357 268
199 101 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7163
AflIII A'CryG_T
Cuts at: 1203 3549 3951 4351 6259 7236 1203
Size: 2346 402 400 1908 977 1327
Fragments arranged by size:
2346 1908 1327 977 402 400
AgeI A'CCGG_T
Cuts at: 6191 6191
Size: 7360
AhdI GACnn_n'nnGTC
Cuts at: 6089 6089
Size: 7360
AleI CACnn'nnGTG
Cuts at: 1166 4334 1166
Size: 3168 4192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7360
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3462 3494 3816 3848 3462
Size: 32 322 32 6974
Fragments arranged by size:
6974 322 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3347 5103 6675 3347
Size: 1756 1572 4032
Fragments arranged by size:
4032 1756 1572
ApaLI G'TGCA_C
Cuts at: 5417 6573 5417
Size: 1156 6204
ApoI r'AATT_y
Cuts at: 1493 4669 4895 5047 7156 1493
Size: 3176 226 152 2109 1697
Fragments arranged by size:
3176 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5914 160
Size: 872 4882 1606
Fragments arranged by size:
4882 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7360
AvaI C'yCGr_G
Cuts at: 1870 1943 2721 3222 4685 1870
Size: 73 778 501 1463 4545
Fragments arranged by size:
4545 1463 778 501 73
AvrII C'CTAG_G
Cuts at: 4315 4315
Size: 7360
BamHI G'GATC_C
Cuts at: 2035 2095 4690 5057 2035
Size: 60 2595 367 4338
Fragments arranged by size:
4338 2595 367 60
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2263 2382 2397 3747
Size: 325 217 627 342 134 119 15 1350
Cuts at: 3747 3769 4645 4681 6136 618
Size: 22 876 36 1455 1842
Fragments arranged by size:
1842 1455 1350 876 627 342 325 217
134 119 36 22 15
BanII G_rGCy'C
Cuts at: 1391 2949 3416 3690 4530 4587 4679 1391
Size: 1558 467 274 840 57 92 4072
Fragments arranged by size:
4072 1558 840 467 274 92 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2175 2411 2429 928
Size: 971 276 236 18 5859
Fragments arranged by size:
5859 971 276 236 18
BbvCI CC'TCA_GC
Cuts at: 3353 4178 3353
Size: 825 6535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3213 3247 3890 3924 5551 5585 1903
Size: 34 1276 34 643 34 1627 34 3678
Fragments arranged by size:
3678 1627 1276 643 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5252 6462 5252
Size: 1210 6150
BclI T'GATC_A
Cuts at: 1548 2442 3931 1548
Size: 894 1489 4977
Fragments arranged by size:
4977 1489 894
BfrBI ATG'CAT
Cuts at: 3845 3845
Size: 7360
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4418 5971 136
Size: 107 122 71 631 605 2746 1553 1525
Fragments arranged by size:
2746 1553 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7355 7355
Size: 7360
BlpI GC'TnA_GC
Cuts at: 3689 4777 3689
Size: 1088 6272
Bme1580I G_kGCm'C
Cuts at: 1790 4147 5421 6577 1790
Size: 2357 1274 1156 2573
Fragments arranged by size:
2573 2357 1274 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2686 2915 4068
Size: 416 53 83 399 24 1269 229 1153
Cuts at: 4068 5091 6049 442
Size: 1023 958 1753
Fragments arranged by size:
1753 1269 1153 1023 958 416 399 229
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7360
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3415 3415
Size: 7360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2539 2666 2978 3210 3260 6020 1239
Size: 1300 127 312 232 50 2760 2579
Fragments arranged by size:
2760 2579 1300 312 232 127 50
Bpu10I CC'TnA_GC
Cuts at: 2345 3353 4178 2345
Size: 1008 825 5527
Fragments arranged by size:
5527 1008 825
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2398 3536 3620 3704 5485 6350 6648 2398
Size: 1138 84 84 1781 865 298 3110
Fragments arranged by size:
3110 1781 1138 865 298 84 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6023 882
Size: 617 4524 2219
Fragments arranged by size:
4524 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4657 5151 493
Size: 711 3453 494 2702
Fragments arranged by size:
3453 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7354 7354
Size: 7360
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 5167 5549 6184
Size: 53 83 186 936 525 3109 382 635
Cuts at: 6184 275
Size: 1451
Fragments arranged by size:
3109 1451 936 635 525 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2206 2400 2568 5793 6191 6465 6612 7023 2206
Size: 194 168 3225 398 274 147 411 2543
Fragments arranged by size:
3225 2543 411 398 274 194 168 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2324 2354 2820 2850 3483
Size: 30 165 30 921 30 466 30 633
Cuts at: 3483 3513 3816 3846 1178
Size: 30 303 30 4692
Fragments arranged by size:
4692 921 633 466 303 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3548 4558 1186
Size: 2362 1010 3988
Fragments arranged by size:
3988 2362 1010
BseYI C'CCAG_C
Cuts at: 2062 3538 4578 6563 7059 2062
Size: 1476 1040 1985 496 2363
Fragments arranged by size:
2363 1985 1476 1040 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 3021 4487 1163
Size: 573 426 859 1466 4036
Fragments arranged by size:
4036 1466 859 573 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3586 4810 5571 6599 1540
Size: 275 186 1585 1224 761 1028 2301
Fragments arranged by size:
2301 1585 1224 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3690 4366 4530 4587 5421 5506 6577 3690
Size: 676 164 57 834 85 1071 4473
Fragments arranged by size:
4473 1071 834 676 164 85 57
BsmI GAATG_Cn'
Cuts at: 4871 4964 4871
Size: 93 7267
BsmBI CGTCTCn'nnnn_
Cuts at: 2183 2344 2910 2183
Size: 161 566 6633
Fragments arranged by size:
6633 566 161
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2949 3416 3690 4147 4366 4530 4587
Size: 399 1159 467 274 457 219 164 57
Cuts at: 4587 4679 5421 5506 6577 1391
Size: 92 742 85 1071 2174
Fragments arranged by size:
2174 1159 1071 742 467 457 399 274
219 164 92 85 57
BspEI T'CCGG_A
Cuts at: 2206 7023 2206
Size: 4817 2543
BspHI T'CATG_A
Cuts at: 2296 5249 6979 2296
Size: 2953 1730 2677
Fragments arranged by size:
2953 2677 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 2394 2777 3094 3355 3892 4715 844
Size: 1290 260 383 317 261 537 823 3489
Fragments arranged by size:
3489 1290 823 537 383 317 261 260
BsrBI CCG'CTC
Cuts at: 1394 1823 5247 1394
Size: 429 3424 3507
Fragments arranged by size:
3507 3424 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3183 5855 6029 65
Size: 1183 1935 2672 174 1396
Fragments arranged by size:
2672 1935 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 6004 6191 1700
Size: 110 244 3950 187 2869
Fragments arranged by size:
3950 2869 244 187 110
BsrGI T'GTAC_A
Cuts at: 96 3621 96
Size: 3525 3835
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7360
BssSI C'ACGA_G
Cuts at: 3039 5420 6432 3039
Size: 2381 1012 3967
Fragments arranged by size:
3967 2381 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3347 794
Size: 2553 4807
BstBI TT'CG_AA
Cuts at: 2007 6164 2007
Size: 4157 3203
BstEII G'GTnAC_C
Cuts at: 1184 3061 4060 1184
Size: 1877 999 4484
Fragments arranged by size:
4484 1877 999
BstXI CCAn_nnnn'nTGG
Cuts at: 3806 4286 3806
Size: 480 6880
BstYI r'GATC_y
Cuts at: 1972 2035 2095 3115 3396 4690 5057 5442 5459
Size: 63 60 1020 281 1294 367 385 17
Cuts at: 5459 6911 6997 7009 7355 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1294 1020 385 367 346 281
86 63 60 17 12
BstZ17I GTA'TAC
Cuts at: 3312 3312
Size: 7360
BtgI C'CryG_G
Cuts at: 513 1065 2022 2512 3057 3827 4413 4441 513
Size: 552 957 490 545 770 586 28 3432
Fragments arranged by size:
3432 957 770 586 552 545 490 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2563 487
Size: 683 56 1337 5284
Fragments arranged by size:
5284 1337 683 56
BtsI GCAGTG_nn'
Cuts at: 2396 3434 4876 5670 5690 2396
Size: 1038 1442 794 20 4066
Fragments arranged by size:
4066 1442 1038 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7325
DraI TTT'AAA
Cuts at: 4664 4664
Size: 7360
DraIII CAC_nnn'GTG
Cuts at: 2598 3501 2598
Size: 903 6457
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6367 809
Size: 507 5051 1802
Fragments arranged by size:
5051 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2749 3151 3325
Size: 54 1425 50 275 18 919 402 174
Cuts at: 3325 3714 4219 4807 5696 7325 8
Size: 389 505 588 889 1629 43
Fragments arranged by size:
1629 1425 919 889 588 505 402 389
275 174 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4807 1537
Size: 275 2995 4090
Fragments arranged by size:
4090 2995 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2306 3384 4641 5290 1353
Size: 759 194 1078 1257 649 3423
Fragments arranged by size:
3423 1257 1078 759 649 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3551 5947 6321 6467 387
Size: 1064 138 1962 2396 374 146 1280
Fragments arranged by size:
2396 1962 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3688 4528 4585 3688
Size: 840 57 6463
Fragments arranged by size:
6463 840 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2539 2666 2978 3107 3210 3260
Size: 393 504 403 127 312 129 103 50
Cuts at: 3260 3908 3965 4322 4521 4622 5423 6020 6807
Size: 648 57 357 199 101 801 597 787
Cuts at: 6807 7075 1239
Size: 268 1524
Fragments arranged by size:
1524 801 787 648 597 504 403 393
357 312 268 199 129 127 103 101
57 50
EcoNI CCTnn'n_nnAGG
Cuts at: 3530 3530
Size: 7360
EcoO109I rG'GnC_Cy
Cuts at: 2028 2680 3730 3811 3877 4070 2028
Size: 652 1050 81 66 193 5318
Fragments arranged by size:
5318 1050 652 193 81 66
EcoRI G'AATT_C
Cuts at: 4669 5047 4669
Size: 378 6982
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4652 4652
Size: 7360
FspI TGC'GCA
Cuts at: 5866 5866
Size: 7360
HaeII r_GCGC'y
Cuts at: 1678 2653 6507 1678
Size: 975 3854 2531
Fragments arranged by size:
3854 2531 975
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 2722 2754 3416
Size: 32 135 32 448 32 670 32 662
Cuts at: 3416 3448 4117 4149 4229 4261 6001 6033 6075
Size: 32 669 32 80 32 1740 32 42
Cuts at: 6075 6107 1373
Size: 32 2626
Fragments arranged by size:
2626 1740 670 669 662 448 135 80
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4704 4950 669
Size: 988 281 2766 246 3079
Fragments arranged by size:
3079 2766 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7360
HpaI GTT'AAC
Cuts at: 4950 4950
Size: 7360
KpnI G_GTAC'C
Cuts at: 2386 2401 3751 4685 2386
Size: 15 1350 934 5061
Fragments arranged by size:
5061 1350 934 15
MfeI C'AATT_G
Cuts at: 4959 4959
Size: 7360
MluI A'CGCG_T
Cuts at: 7236 7236
Size: 7360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3026 6474 6658 1305
Size: 1721 3448 184 2007
Fragments arranged by size:
3448 2007 1721 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2751 3153 3327 3716 10
Size: 54 1425 343 919 402 174 389 3654
Fragments arranged by size:
3654 1425 919 402 389 343 174 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2438 3645 4334 5318 5677 5836 518
Size: 648 1272 1207 689 984 359 159 2042
Fragments arranged by size:
2042 1272 1207 984 689 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2224 2670 3136 4106 4776 5453 6601 6846
Size: 618 446 466 970 670 677 1148 245
Cuts at: 6846 1606
Size: 2120
Fragments arranged by size:
2120 1148 970 677 670 618 466 446
245
NcoI C'CATG_G
Cuts at: 513 1065 2022 3827 4413 4441 513
Size: 552 957 1805 586 28 3432
Fragments arranged by size:
3432 1805 957 586 552 28
NdeI CA'TA_TG
Cuts at: 387 3631 387
Size: 3244 4116
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7360
NotI GC'GGCC_GC
Cuts at: 4807 4807
Size: 7360
NsiI A_TGCA'T
Cuts at: 3847 3847
Size: 7360
NspI r_CATG'y
Cuts at: 1132 1229 3265 3553 3622 3955 4355 4718 6263
Size: 97 2036 288 69 333 400 363 1545
Cuts at: 6263 1132
Size: 2229
Fragments arranged by size:
2229 2036 1545 400 363 333 288 97
69
PasI CC'CwG_GG
Cuts at: 1348 2927 1348
Size: 1579 5781
PciI A'CATG_T
Cuts at: 3549 3951 4351 6259 3549
Size: 402 400 1908 4650
Fragments arranged by size:
4650 1908 402 400
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4354 4578 1108
Size: 3246 224 3890
Fragments arranged by size:
3890 3246 224
PfoI T'CCnGG_A
Cuts at: 4155 4155
Size: 7360
PmeI GTTT'AAAC
Cuts at: 4664 4664
Size: 7360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3816 3848 5429 5461 6942 6974 3816
Size: 32 1581 32 1481 32 4202
Fragments arranged by size:
4202 1581 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2028 3730 3811 3877 2028
Size: 1702 81 66 5511
Fragments arranged by size:
5511 1702 81 66
PshAI GACnn'nnGTC
Cuts at: 1613 3582 1613
Size: 1969 5391
PsiI TTA'TAA
Cuts at: 4930 4930
Size: 7360
PspXI vC'TCGA_Gb
Cuts at: 3222 3222
Size: 7360
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 7328
PstI C_TGCA'G
Cuts at: 830 2391 3903 4712 830
Size: 1561 1512 809 3478
Fragments arranged by size:
3478 1561 1512 809
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7360
PvuII CAG'CTG
Cuts at: 2224 2670 3136 4106 2224
Size: 446 466 970 5478
Fragments arranged by size:
5478 970 466 446
SacI G_AGCT'C
Cuts at: 3690 4530 4587 3690
Size: 840 57 6463
Fragments arranged by size:
6463 840 57
SalI G'TCGA_C
Cuts at: 1936 4702 1936
Size: 2766 4594
SanDI GG'GwC_CC
Cuts at: 2028 3811 2028
Size: 1783 5577
SapI GCTCTTCn'nnn_
Cuts at: 4641 4641
Size: 7360
SbfI CC_TGCA'GG
Cuts at: 4712 4712
Size: 7360
ScaI AGT'ACT
Cuts at: 5608 7320 5608
Size: 1712 5648
SexAI A'CCwGG_T
Cuts at: 2259 2259
Size: 7360
SfcI C'TryA_G
Cuts at: 826 1045 2387 2823 3301 3695 3899 4708 5843
Size: 219 1342 436 478 394 204 809 1135
Cuts at: 5843 6524 6715 826
Size: 681 191 1471
Fragments arranged by size:
1471 1342 1135 809 681 478 436 394
219 204 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7360
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7360
SmaI CCC'GGG
Cuts at: 1872 4687 1872
Size: 2815 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2377 3222 3515 3599 3683 5464
Size: 197 926 434 845 293 84 84 1781
Cuts at: 5464 6365 6627 820
Size: 901 262 1553
Fragments arranged by size:
1781 1553 926 901 845 434 293 262
197 84 84
SnaBI TAC'GTA
Cuts at: 493 4657 5151 493
Size: 4164 494 2702
Fragments arranged by size:
4164 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7360
SphI G_CATG'C
Cuts at: 1132 4718 1132
Size: 3586 3774
SspI AAT'ATT
Cuts at: 5 52 5284 5
Size: 47 5232 2081
Fragments arranged by size:
5232 2081 47
StuI AGG'CCT
Cuts at: 1517 4307 4397 1517
Size: 2790 90 4480
Fragments arranged by size:
4480 2790 90
StyI C'CwwG_G
Cuts at: 513 1065 2022 2133 2481 3827 4315 4413 4441
Size: 552 957 111 348 1346 488 98 28
Cuts at: 4441 4799 513
Size: 358 3074
Fragments arranged by size:
3074 1346 957 552 488 358 348 111
98 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2474 2800 2897 4048 4474 4478 5387
Size: 1255 894 326 97 1151 426 4 909
Cuts at: 5387 5404 5557 5742 6187 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1151 909 894 445 426 326
185 153 97 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3621 5606 7318
Size: 274 80 33 51 1443 1644 1985 1712
Cuts at: 7318 96
Size: 138
Fragments arranged by size:
1985 1712 1644 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2820 2852 3483 3515 4137 4169 7320 7352 2820
Size: 32 631 32 622 32 3151 32 2828
Fragments arranged by size:
3151 2828 631 622 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7360
XbaI T'CTAG_A
Cuts at: 2869 4696 2869
Size: 1827 5533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2934 4049 4621 2934
Size: 1115 572 5673
Fragments arranged by size:
5673 1115 572
XhoI C'TCGA_G
Cuts at: 1943 3222 1943
Size: 1279 6081
XmaI C'CCGG_G
Cuts at: 1870 4685 1870
Size: 2815 4545
XmnI GAAnn'nnTTC
Cuts at: 5489 5489
Size: 7360
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5168 276
Size: 53 83 186 936 3634 2468
Fragments arranged by size:
3634 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BsiWI Bsu36I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI RsrII SacII SfiI SfoI SrfI SwaI