Restriction map for
pFN21ASDA1599
(Circular) (Six-base) MAPSORT of: pFN21ASDA1599.seq Check: 2319 from: 1 to: 6508
pFN21ASDA1599 6508 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4318 278
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2171 3851 1937
Size: 234 1680 4594
Fragments arranged by size:
4594 1680 234
Acc65I G'GTAC_C
Cuts at: 3829 3829
Size: 6508
AclI AA'CG_TT
Cuts at: 1563 2654 4635 5008 1563
Size: 1091 1981 373 3063
Fragments arranged by size:
3063 1981 1091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4571 5955 6223 1632
Size: 2939 1384 268 1917
Fragments arranged by size:
2939 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6311
AflIII A'CryG_T
Cuts at: 1203 2136 5407 6384 1203
Size: 933 3271 977 1327
Fragments arranged by size:
3271 1327 977 933
AgeI A'CCGG_T
Cuts at: 5339 5339
Size: 6508
AhdI GACnn_n'nnGTC
Cuts at: 2044 5237 2044
Size: 3193 3315
AleI CACnn'nnGTG
Cuts at: 1166 2093 3341 1166
Size: 927 1248 4333
Fragments arranged by size:
4333 1248 927
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2483 2589 3644 1184
Size: 1299 106 1055 4048
Fragments arranged by size:
4048 1299 1055 106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2639 2671 2639
Size: 32 6476
AlwNI CAG_nnn'CTG
Cuts at: 2544 4251 5823 2544
Size: 1707 1572 3229
Fragments arranged by size:
3229 1707 1572
ApaLI G'TGCA_C
Cuts at: 2965 4565 5721 2965
Size: 1600 1156 3752
Fragments arranged by size:
3752 1600 1156
ApoI r'AATT_y
Cuts at: 1493 2446 2920 3817 4043 4195 6304 1493
Size: 953 474 897 226 152 2109 1697
Fragments arranged by size:
2109 1697 953 897 474 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5062 160
Size: 872 4030 1606
Fragments arranged by size:
4030 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
AvaI C'yCGr_G
Cuts at: 1870 1943 3552 3651 3833 1870
Size: 73 1609 99 182 4545
Fragments arranged by size:
4545 1609 182 99 73
BamHI G'GATC_C
Cuts at: 3838 4205 3838
Size: 367 6141
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2510 3829 5284 618
Size: 325 217 627 723 1319 1455 1842
Fragments arranged by size:
1842 1455 1319 723 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2538 2737 3080 3827 1391
Size: 1147 199 343 747 4072
Fragments arranged by size:
4072 1147 747 343 199
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5537
BbvCI CC'TCA_GC
Cuts at: 2529 2529
Size: 6508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4699 4733 1903
Size: 34 2762 34 3678
Fragments arranged by size:
3678 2762 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4400 5610 4400
Size: 1210 5298
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6508
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3647 5119 136
Size: 107 122 71 631 605 1975 1472 1525
Fragments arranged by size:
1975 1525 1472 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6503 6503
Size: 6508
BlpI GC'TnA_GC
Cuts at: 2051 2537 3632 3925 2051
Size: 486 1095 293 4634
Fragments arranged by size:
4634 1095 486 293
Bme1580I G_kGCm'C
Cuts at: 1790 2969 4569 5725 1790
Size: 1179 1600 1156 2573
Fragments arranged by size:
2573 1600 1179 1156
BmgBI CAC'GTC
Cuts at: 1318 3538 3658 1318
Size: 2220 120 4168
Fragments arranged by size:
4168 2220 120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2794 3373 4239
Size: 416 53 83 399 24 1377 579 866
Cuts at: 4239 5197 442
Size: 958 1753
Fragments arranged by size:
1753 1377 958 866 579 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6508
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2080 3259 2080
Size: 1179 5329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3290 5168 1239
Size: 2051 1878 2579
Fragments arranged by size:
2579 2051 1878
Bpu10I CC'TnA_GC
Cuts at: 2529 2529
Size: 6508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4633 5498 5796 4633
Size: 865 298 5345
Fragments arranged by size:
5345 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5171 882
Size: 617 3672 2219
Fragments arranged by size:
3672 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2917 3343 3607 3628 3805 4299 493
Size: 711 1713 426 264 21 177 494 2702
Fragments arranged by size:
2702 1713 711 494 426 264 177 21
BsaBI GATnn'nnATC
Cuts at: 6502 6502
Size: 6508
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 3510 4315 4697
Size: 53 83 186 936 512 1465 805 382
Cuts at: 4697 5332 275
Size: 635 1451
Fragments arranged by size:
1465 1451 936 805 635 512 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4941 5339 5613 5760 6171 4941
Size: 398 274 147 411 5278
Fragments arranged by size:
5278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2373 2403 2948 2978 1178
Size: 30 165 30 970 30 545 30 4708
Fragments arranged by size:
4708 970 545 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2546 3538 1186
Size: 1111 249 992 4156
Fragments arranged by size:
4156 1111 992 249
BseYI C'CCAG_C
Cuts at: 3760 5711 6207 3760
Size: 1951 496 4061
Fragments arranged by size:
4061 1951 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2356 3050 3320 3386 3773 1163
Size: 573 620 694 270 66 387 3898
Fragments arranged by size:
3898 694 620 573 387 270 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2758 3363 3958 4719 5747 1540
Size: 275 186 757 605 595 761 1028 2301
Fragments arranged by size:
2301 1028 761 757 605 595 275 186
BsiHKAI G_wGCw'C
Cuts at: 2538 2969 4569 4654 5725 2538
Size: 431 1600 85 1071 3321
Fragments arranged by size:
3321 1600 1071 431 85
BsmI GAATG_Cn'
Cuts at: 2435 3358 3708 4019 4112 2435
Size: 923 350 311 93 4831
Fragments arranged by size:
4831 923 350 311 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3648 3648
Size: 6508
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2538 2737 2969 3080 3827 4569 4654
Size: 399 748 199 232 111 747 742 85
Cuts at: 4654 5725 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 748 747 742 399 232 199
111 85
BspEI T'CCGG_A
Cuts at: 6171 6171
Size: 6508
BspHI T'CATG_A
Cuts at: 2682 4397 6127 2682
Size: 1715 1730 3063
Fragments arranged by size:
3063 1730 1715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3863 844
Size: 3019 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4395 1394
Size: 429 2572 3507
Fragments arranged by size:
3507 2572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5003 5177 65
Size: 1183 3755 174 1396
Fragments arranged by size:
3755 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5152 5339 1700
Size: 110 3342 187 2869
Fragments arranged by size:
3342 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6508
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6508
BssSI C'ACGA_G
Cuts at: 3209 4568 5580 3209
Size: 1359 1012 4137
Fragments arranged by size:
4137 1359 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6508
BstBI TT'CG_AA
Cuts at: 2007 5312 2007
Size: 3305 3203
BstEII G'GTnAC_C
Cuts at: 1184 3334 1184
Size: 2150 4358
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 2584 2770 2095
Size: 489 186 5833
Fragments arranged by size:
5833 489 186
BstYI r'GATC_y
Cuts at: 1972 3543 3669 3838 4205 4590 4607 6059 6145
Size: 1571 126 169 367 385 17 1452 86
Cuts at: 6145 6157 6503 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1571 1452 385 367 346 169 126
86 17 12
BstZ17I GTA'TAC
Cuts at: 2172 2172
Size: 6508
Bsu36I CC'TnA_GG
Cuts at: 2034 2034
Size: 6508
BtgI C'CryG_G
Cuts at: 513 1065 2022 2848 3330 3513 513
Size: 552 957 826 482 183 3508
Fragments arranged by size:
3508 957 826 552 482 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5769
Fragments arranged by size:
5769 683 56
BtsI GCAGTG_nn'
Cuts at: 2201 3027 4024 4818 4838 2201
Size: 826 997 794 20 3871
Fragments arranged by size:
3871 997 826 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3304 3339 529
Size: 35 2740 35 3698
Fragments arranged by size:
3698 2740 35 35
DraI TTT'AAA
Cuts at: 3812 3812
Size: 6508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5515 809
Size: 507 4199 1802
Fragments arranged by size:
4199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 3015 3345
Size: 54 1425 50 275 18 255 930 330
Cuts at: 3345 3360 3955 4844 6473 8
Size: 15 595 889 1629 43
Fragments arranged by size:
1629 1425 930 889 595 330 275 255
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3360 3955 1537
Size: 275 1548 595 4090
Fragments arranged by size:
4090 1548 595 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4438 1353
Size: 3085 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5095 5469 5615 387
Size: 1064 138 3506 374 146 1280
Fragments arranged by size:
3506 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2536 2536
Size: 6508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3290 4571 5168 5955 6223 1239
Size: 393 1658 1281 597 787 268 1524
Fragments arranged by size:
1658 1524 1281 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 6508
EcoO109I rG'GnC_Cy
Cuts at: 2394 2994 2394
Size: 600 5908
EcoRI G'AATT_C
Cuts at: 2446 2920 3817 4195 2446
Size: 474 897 378 4759
Fragments arranged by size:
4759 897 474 378
FspI TGC'GCA
Cuts at: 2255 3293 5014 2255
Size: 1038 1721 3749
Fragments arranged by size:
3749 1721 1038
HaeII r_GCGC'y
Cuts at: 1678 3110 5655 1678
Size: 1432 2545 2531
Fragments arranged by size:
2545 2531 1432
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2306 2338 3114
Size: 32 135 32 458 32 244 32 776
Cuts at: 3114 3146 5149 5181 5223 5255 1373
Size: 32 2003 32 42 32 2626
Fragments arranged by size:
2626 2003 776 458 244 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 3451 3852 4098 669
Size: 988 281 382 1131 401 246 3079
Fragments arranged by size:
3079 1131 988 401 382 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6508
HpaI GTT'AAC
Cuts at: 4098 4098
Size: 6508
KpnI G_GTAC'C
Cuts at: 3833 3833
Size: 6508
MfeI C'AATT_G
Cuts at: 4107 4107
Size: 6508
MluI A'CGCG_T
Cuts at: 6384 6384
Size: 6508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2175 2505 3100 5622 5806 1305
Size: 870 330 595 2522 184 2007
Fragments arranged by size:
2522 2007 870 595 330 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3017 3347 10
Size: 54 1425 343 1185 330 3171
Fragments arranged by size:
3171 1425 1185 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 2726 2768 3341 4466 4825 4984
Size: 648 927 633 42 573 1125 359 159
Cuts at: 4984 518
Size: 2042
Fragments arranged by size:
2042 1125 927 648 633 573 359 159
42
MspA1I CmG'CkG
Cuts at: 1606 2474 2528 3088 3304 3359 3924 4601 5749
Size: 868 54 560 216 55 565 677 1148
Cuts at: 5749 5994 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 868 677 565 560 245 216
55 54
NcoI C'CATG_G
Cuts at: 513 1065 2022 3513 513
Size: 552 957 1491 3508
Fragments arranged by size:
3508 1491 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6508
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6508
NotI GC'GGCC_GC
Cuts at: 3955 3955
Size: 6508
NspI r_CATG'y
Cuts at: 1132 1229 2140 3866 5411 1132
Size: 97 911 1726 1545 2229
Fragments arranged by size:
2229 1726 1545 911 97
PasI CC'CwG_GG
Cuts at: 1348 2399 3166 1348
Size: 1051 767 4690
Fragments arranged by size:
4690 1051 767
PciI A'CATG_T
Cuts at: 2136 5407 2136
Size: 3271 3237
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2182 3224 1108
Size: 1074 1042 4392
Fragments arranged by size:
4392 1074 1042
PfoI T'CCnGG_A
Cuts at: 2124 2450 3267 3597 2124
Size: 326 817 330 5035
Fragments arranged by size:
5035 817 330 326
PmeI GTTT'AAAC
Cuts at: 3812 3812
Size: 6508
PmlI CAC'GTG
Cuts at: 3343 3628 3343
Size: 285 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4577 4609 6090 6122 4577
Size: 32 1481 32 4963
Fragments arranged by size:
4963 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2394 2994 2394
Size: 600 5908
PshAI GACnn'nnGTC
Cuts at: 1613 2678 3116 1613
Size: 1065 438 5005
Fragments arranged by size:
5005 1065 438
PsiI TTA'TAA
Cuts at: 4078 4078
Size: 6508
PstI C_TGCA'G
Cuts at: 830 2117 3860 830
Size: 1287 1743 3478
Fragments arranged by size:
3478 1743 1287
PvuI CG_AT'CG
Cuts at: 2001 2758 2001
Size: 757 5751
PvuII CAG'CTG
Cuts at: 2474 3088 3304 2474
Size: 614 216 5678
Fragments arranged by size:
5678 614 216
SacI G_AGCT'C
Cuts at: 2538 2538
Size: 6508
SalI G'TCGA_C
Cuts at: 1936 3850 1936
Size: 1914 4594
SanDI GG'GwC_CC
Cuts at: 2394 2994 2394
Size: 600 5908
SbfI CC_TGCA'GG
Cuts at: 2117 3860 2117
Size: 1743 4765
ScaI AGT'ACT
Cuts at: 3383 4756 6468 3383
Size: 1373 1712 3423
Fragments arranged by size:
3423 1712 1373
SfcI C'TryA_G
Cuts at: 826 1045 2113 2545 2632 3856 4991 5672 5863
Size: 219 1068 432 87 1224 1135 681 191
Cuts at: 5863 826
Size: 1471
Fragments arranged by size:
1471 1224 1135 1068 681 432 219 191
87
SfiI GGCCn_nnn'nGGCC
Cuts at: 3647 3647
Size: 6508
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6508
SmaI CCC'GGG
Cuts at: 1872 3554 3835 1872
Size: 1682 281 4545
Fragments arranged by size:
4545 1682 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4612 5513 5775 820
Size: 197 926 2669 901 262 1553
Fragments arranged by size:
2669 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3805 4299 493
Size: 3312 494 2702
Fragments arranged by size:
3312 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6508
SphI G_CATG'C
Cuts at: 1132 3866 1132
Size: 2734 3774
SspI AAT'ATT
Cuts at: 5 52 4432 5
Size: 47 4380 2081
Fragments arranged by size:
4380 2081 47
StuI AGG'CCT
Cuts at: 1517 2366 3275 1517
Size: 849 909 4750
Fragments arranged by size:
4750 909 849
StyI C'CwwG_G
Cuts at: 513 1065 2022 2067 2091 2176 2377 3435 3513
Size: 552 957 45 24 85 201 1058 78
Cuts at: 3513 3947 513
Size: 434 3074
Fragments arranged by size:
3074 1058 957 552 434 201 85 78
45 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2690 3353 4535 4552 4705 4890 5335
Size: 1255 1110 663 1182 17 153 185 445
Cuts at: 5335 325
Size: 1498
Fragments arranged by size:
1498 1255 1182 1110 663 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3381 4754 6466
Size: 274 80 33 51 1443 1404 1373 1712
Cuts at: 6466 96
Size: 138
Fragments arranged by size:
1712 1443 1404 1373 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2224 2256 2373 2405 2683 2715 2948 2980 6468
Size: 32 117 32 278 32 233 32 3488
Cuts at: 6468 6500 2224
Size: 32 2232
Fragments arranged by size:
3488 2232 278 233 117 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3578 1316
Size: 2262 4246
XbaI T'CTAG_A
Cuts at: 3844 3844
Size: 6508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2810 3520 2810
Size: 710 5798
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6508
XmaI C'CCGG_G
Cuts at: 1870 3552 3833 1870
Size: 1682 281 4545
Fragments arranged by size:
4545 1682 281
XmnI GAAnn'nnTTC
Cuts at: 2597 4637 2597
Size: 2040 4468
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4316 276
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacII SapI SexAI SfoI SrfI SwaI