Restriction map for
pFN21ASDA1589
(Circular) (Six-base) MAPSORT of: pFN21ASDA1589.seq Check: 5421 from: 1 to: 7060
pFN21ASDA1589 7060 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3314 3314
Size: 7060
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3851 4870 278
Size: 53 83 186 936 2315 1019 2468
Fragments arranged by size:
2468 2315 1019 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2484 3431 4403 1937
Size: 547 947 972 4594
Fragments arranged by size:
4594 972 947 547
Acc65I G'GTAC_C
Cuts at: 4381 4381
Size: 7060
AclI AA'CG_TT
Cuts at: 1563 5187 5560 1563
Size: 3624 373 3063
Fragments arranged by size:
3624 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2906 3096 3226 3242 3692 5123 6507 6775
Size: 1274 190 130 16 450 1431 1384 268
Cuts at: 6775 1632
Size: 1917
Fragments arranged by size:
1917 1431 1384 1274 450 268 190 130
16
AflII C'TTAA_G
Cuts at: 820 1017 2783 820
Size: 197 1766 5097
Fragments arranged by size:
5097 1766 197
AflIII A'CryG_T
Cuts at: 1203 5959 6936 1203
Size: 4756 977 1327
Fragments arranged by size:
4756 1327 977
AgeI A'CCGG_T
Cuts at: 3048 5891 3048
Size: 2843 4217
AhdI GACnn_n'nnGTC
Cuts at: 5789 5789
Size: 7060
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7060
AlwNI CAG_nnn'CTG
Cuts at: 2560 3001 3700 3826 3938 4803 6375 2560
Size: 441 699 126 112 865 1572 3245
Fragments arranged by size:
3245 1572 865 699 441 126 112
ApaI G_GGCC'C
Cuts at: 2960 3956 2960
Size: 996 6064
ApaLI G'TGCA_C
Cuts at: 2987 5117 6273 2987
Size: 2130 1156 3774
Fragments arranged by size:
3774 2130 1156
ApoI r'AATT_y
Cuts at: 1493 2499 2855 4369 4595 4747 6856 1493
Size: 1006 356 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 1006 356 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5614 160
Size: 872 4582 1606
Fragments arranged by size:
4582 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
AvaI C'yCGr_G
Cuts at: 1870 1943 3199 4385 1870
Size: 73 1256 1186 4545
Fragments arranged by size:
4545 1256 1186 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3609 3642 3609
Size: 33 7027
BamHI G'GATC_C
Cuts at: 4390 4757 4390
Size: 367 6693
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2295 3025 3195 4159 4381
Size: 325 217 627 508 730 170 964 222
Cuts at: 4381 5836 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 964 730 627 508 325 222
217 170
BanII G_rGCy'C
Cuts at: 1391 2169 2739 2907 2960 3008 3956 4379 1391
Size: 778 570 168 53 48 948 423 4072
Fragments arranged by size:
4072 948 778 570 423 168 53 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2526 928
Size: 971 627 5462
Fragments arranged by size:
5462 971 627
BbvCI CC'TCA_GC
Cuts at: 3323 3323
Size: 7060
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5251 5285 1903
Size: 34 3314 34 3678
Fragments arranged by size:
3678 3314 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3610 4952 6162 3610
Size: 1342 1210 4508
Fragments arranged by size:
4508 1342 1210
BclI T'GATC_A
Cuts at: 1548 2551 3010 1548
Size: 1003 459 5598
Fragments arranged by size:
5598 1003 459
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3224 5671 136
Size: 107 122 71 631 605 1552 2447 1525
Fragments arranged by size:
2447 1552 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7055 7055
Size: 7060
BlpI GC'TnA_GC
Cuts at: 3743 4477 3743
Size: 734 6326
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2960 2991 3480 3956 5121 6277 1790
Size: 242 928 31 489 476 1165 1156 2573
Fragments arranged by size:
2573 1165 1156 928 489 476 242 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7060
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2696 2963 3011
Size: 416 53 83 399 24 1279 267 48
Cuts at: 3011 4165 4791 5749 442
Size: 1154 626 958 1753
Fragments arranged by size:
1753 1279 1154 958 626 416 399 267
83 53 48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2384 2432 3826 5720 1239
Size: 1106 39 48 1394 1894 2579
Fragments arranged by size:
2579 1894 1394 1106 48 39
Bpu10I CC'TnA_GC
Cuts at: 3323 3939 3323
Size: 616 6444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2891 5185 6050 6348 2891
Size: 2294 865 298 3603
Fragments arranged by size:
3603 2294 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2255 2429 2975 3521 5723 882
Size: 617 756 174 546 546 2202 2219
Fragments arranged by size:
2219 2202 756 617 546 546 174
BsaAI yAC'GTr
Cuts at: 493 1204 4357 4851 493
Size: 711 3153 494 2702
Fragments arranged by size:
3153 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2556 7054 2556
Size: 4498 2562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3848 4867 5249 5884
Size: 53 83 186 936 2315 1019 382 635
Cuts at: 5884 275
Size: 1451
Fragments arranged by size:
2315 1451 1019 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3048 3054 5493 5891 6165 6312 6723 3048
Size: 6 2439 398 274 147 411 3385
Fragments arranged by size:
3385 2439 411 398 274 147 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3172 3202 4083 4113 1178
Size: 30 165 30 1769 30 881 30 4125
Fragments arranged by size:
4125 1769 881 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2177 2447 3301 1186
Size: 988 3 270 854 4945
Fragments arranged by size:
4945 988 854 270 3
BseYI C'CCAG_C
Cuts at: 2041 2994 3061 3693 3948 4299 6263 6759 2041
Size: 953 67 632 255 351 1964 496 2342
Fragments arranged by size:
2342 1964 953 632 496 351 255 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2656 4097 1163
Size: 573 920 1441 4126
Fragments arranged by size:
4126 1441 920 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4510 5271 6299 1540
Size: 275 186 2509 761 1028 2301
Fragments arranged by size:
2509 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 2907 2991 4268 5121 5206 6277 2169
Size: 738 84 1277 853 85 1071 2952
Fragments arranged by size:
2952 1277 1071 853 738 85 84
BsmI GAATG_Cn'
Cuts at: 4571 4664 4571
Size: 93 6967
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2169 2739 2907 2960 2991 3008
Size: 399 242 137 570 168 53 31 17
Cuts at: 3008 3480 3956 4268 4379 5121 5206 6277 1391
Size: 472 476 312 111 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 476 472 399 312
242 168 137 111 85 53 31 17
BspEI T'CCGG_A
Cuts at: 3054 6723 3054
Size: 3669 3391
BspHI T'CATG_A
Cuts at: 3379 4949 6679 3379
Size: 1570 1730 3760
Fragments arranged by size:
3760 1730 1570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2312 3314 3443 4415 844
Size: 1468 1002 129 972 3489
Fragments arranged by size:
3489 1468 1002 972 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2200 3943 4947 1394
Size: 429 377 1743 1004 3507
Fragments arranged by size:
3507 1743 1004 429 377
BsrDI GCAATG_nn'
Cuts at: 65 1248 4050 5555 5729 65
Size: 1183 2802 1505 174 1396
Fragments arranged by size:
2802 1505 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2832 3048 5704 5891 1700
Size: 110 1022 216 2656 187 2869
Fragments arranged by size:
2869 2656 1022 216 187 110
BsrGI T'GTAC_A
Cuts at: 96 2382 96
Size: 2286 4774
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7060
BssSI C'ACGA_G
Cuts at: 4293 5120 6132 4293
Size: 827 1012 5221
Fragments arranged by size:
5221 1012 827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3444 3527 794
Size: 2650 83 4327
Fragments arranged by size:
4327 2650 83
BstBI TT'CG_AA
Cuts at: 2007 5864 2007
Size: 3857 3203
BstEII G'GTnAC_C
Cuts at: 1184 4346 1184
Size: 3162 3898
BstYI r'GATC_y
Cuts at: 1972 3252 3607 4390 4757 5142 5159 6611 6697
Size: 1280 355 783 367 385 17 1452 86
Cuts at: 6697 6709 7055 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1280 783 385 367 355 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3432 3432
Size: 7060
BtgI C'CryG_G
Cuts at: 513 1065 3126 4095 513
Size: 552 2061 969 3478
Fragments arranged by size:
3478 2061 969 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6321
Fragments arranged by size:
6321 683 56
BtsI GCAGTG_nn'
Cuts at: 2150 2724 2916 3877 4180 4228 4576 5370 5390
Size: 574 192 961 303 48 348 794 20
Cuts at: 5390 2150
Size: 3820
Fragments arranged by size:
3820 961 794 574 348 303 192 48
20
ClaI AT'CG_AT
Cuts at: 1554 2724 1554
Size: 1170 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7025
DraI TTT'AAA
Cuts at: 2647 3856 4364 2647
Size: 1209 508 5343
Fragments arranged by size:
5343 1209 508
DraIII CAC_nnn'GTG
Cuts at: 3233 3233
Size: 7060
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2665 4310 6067 809
Size: 507 1349 1645 1757 1802
Fragments arranged by size:
1802 1757 1645 1349 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2218 2793 3929
Size: 54 1425 50 275 18 388 575 1136
Cuts at: 3929 4140 4507 5396 7025 8
Size: 211 367 889 1629 43
Fragments arranged by size:
1629 1425 1136 889 575 388 367 275
211 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4507 1537
Size: 275 2695 4090
Fragments arranged by size:
4090 2695 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2330 2415 2855 4990 1353
Size: 977 85 440 2135 3423
Fragments arranged by size:
3423 2135 977 440 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5647 6021 6167 387
Size: 1064 138 4058 374 146 1280
Fragments arranged by size:
4058 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2167 2905 2167
Size: 738 6322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2384 2432 2906 3096 3226 3242
Size: 393 713 39 48 474 190 130 16
Cuts at: 3242 3692 3826 5123 5720 6507 6775 1239
Size: 450 134 1297 597 787 268 1524
Fragments arranged by size:
1524 1297 787 713 597 474 450 393
268 190 134 130 48 39 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2243 3805 2243
Size: 1562 5498
EcoO109I rG'GnC_Cy
Cuts at: 2053 2788 2957 3952 3953 2053
Size: 735 169 995 1 5160
Fragments arranged by size:
5160 995 735 169 1
EcoRI G'AATT_C
Cuts at: 4369 4747 4369
Size: 378 6682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2106 2106
Size: 7060
FspI TGC'GCA
Cuts at: 5566 5566
Size: 7060
HaeII r_GCGC'y
Cuts at: 1678 2185 3700 6207 1678
Size: 507 1515 2507 2531
Fragments arranged by size:
2531 2507 1515 507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 3002 3034 3538
Size: 32 135 32 1013 32 385 32 504
Cuts at: 3538 3570 4250 4282 5701 5733 5775 5807 1373
Size: 32 680 32 1419 32 42 32 2626
Fragments arranged by size:
2626 1419 1013 680 504 385 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4404 4650 669
Size: 988 281 2466 246 3079
Fragments arranged by size:
3079 2466 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2877 748
Size: 2129 4931
HpaI GTT'AAC
Cuts at: 4650 4650
Size: 7060
KpnI G_GTAC'C
Cuts at: 4385 4385
Size: 7060
MfeI C'AATT_G
Cuts at: 4659 4659
Size: 7060
MluI A'CGCG_T
Cuts at: 6936 6936
Size: 7060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3316 3684 6174 6358 1305
Size: 2011 368 2490 184 2007
Fragments arranged by size:
2490 2011 2007 368 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2220 2795 10
Size: 54 1425 343 388 575 4275
Fragments arranged by size:
4275 1425 575 388 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3414 5018 5377 5536 518
Size: 648 2248 1604 359 159 2042
Fragments arranged by size:
2248 2042 1604 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2097 2154 3246 3327 3396 3912 4476 5153
Size: 491 57 1092 81 69 516 564 677
Cuts at: 5153 6301 6546 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1092 677 564 516 491 245
81 69 57
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6508
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7060
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7060
NotI GC'GGCC_GC
Cuts at: 4507 4507
Size: 7060
NspI r_CATG'y
Cuts at: 1132 1229 3647 4418 5963 1132
Size: 97 2418 771 1545 2229
Fragments arranged by size:
2418 2229 1545 771 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7060
PciI A'CATG_T
Cuts at: 5959 5959
Size: 7060
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3001 1108
Size: 1893 5167
PfoI T'CCnGG_A
Cuts at: 2400 3042 2400
Size: 642 6418
PmeI GTTT'AAAC
Cuts at: 4364 4364
Size: 7060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5129 5161 6642 6674 5129
Size: 32 1481 32 5515
Fragments arranged by size:
5515 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2788 2788
Size: 7060
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7060
PsiI TTA'TAA
Cuts at: 4630 4630
Size: 7060
PspOMI G'GGCC_C
Cuts at: 2956 3952 2956
Size: 996 6064
PstI C_TGCA'G
Cuts at: 830 2138 2145 2965 3401 4412 830
Size: 1308 7 820 436 1011 3478
Fragments arranged by size:
3478 1308 1011 820 436 7
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7060
PvuII CAG'CTG
Cuts at: 3327 3912 3327
Size: 585 6475
SacI G_AGCT'C
Cuts at: 2169 2907 2169
Size: 738 6322
SalI G'TCGA_C
Cuts at: 1936 4402 1936
Size: 2466 4594
SbfI CC_TGCA'GG
Cuts at: 4412 4412
Size: 7060
ScaI AGT'ACT
Cuts at: 3590 5308 7020 3590
Size: 1718 1712 3630
Fragments arranged by size:
3630 1718 1712
SexAI A'CCwGG_T
Cuts at: 2266 2266
Size: 7060
SfcI C'TryA_G
Cuts at: 826 1045 2134 2141 2961 3397 4408 5543 6224
Size: 219 1089 7 820 436 1011 1135 681
Cuts at: 6224 6415 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1089 1011 820 681 436 219
191 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7060
SmaI CCC'GGG
Cuts at: 1872 3201 4387 1872
Size: 1329 1186 4545
Fragments arranged by size:
4545 1329 1186
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2783 2906 5164 6065 6327 820
Size: 197 926 840 123 2258 901 262 1553
Fragments arranged by size:
2258 1553 926 901 840 262 197 123
SnaBI TAC'GTA
Cuts at: 493 4357 4851 493
Size: 3864 494 2702
Fragments arranged by size:
3864 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7060
SphI G_CATG'C
Cuts at: 1132 4418 1132
Size: 3286 3774
SspI AAT'ATT
Cuts at: 5 52 4984 5
Size: 47 4932 2081
Fragments arranged by size:
4932 2081 47
StuI AGG'CCT
Cuts at: 1517 3443 4147 1517
Size: 1926 704 4430
Fragments arranged by size:
4430 1926 704
StyI C'CwwG_G
Cuts at: 513 1065 2860 2898 3748 3759 4499 513
Size: 552 1795 38 850 11 740 3074
Fragments arranged by size:
3074 1795 850 740 552 38 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3008 4147 5087 5104 5257 5442 5887
Size: 1255 1428 1139 940 17 153 185 445
Cuts at: 5887 325
Size: 1498
Fragments arranged by size:
1498 1428 1255 1139 940 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2382 3588 5306
Size: 274 80 33 51 1443 405 1206 1718
Cuts at: 5306 7018 96
Size: 1712 138
Fragments arranged by size:
1718 1712 1443 1206 405 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3172 3204 4083 4115 7020 7052 3172
Size: 32 879 32 2905 32 3180
Fragments arranged by size:
3180 2905 879 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2807 1316
Size: 1491 5569
XbaI T'CTAG_A
Cuts at: 4396 4396
Size: 7060
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7060
XmaI C'CCGG_G
Cuts at: 1870 3199 4385 1870
Size: 1329 1186 4545
Fragments arranged by size:
4545 1329 1186
XmnI GAAnn'nnTTC
Cuts at: 2499 2891 4202 5189 2499
Size: 392 1311 987 4370
Fragments arranged by size:
4370 1311 987 392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3849 4868 276
Size: 53 83 186 936 2315 1019 2468
Fragments arranged by size:
2468 2315 1019 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstXI Bsu36I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI