Restriction map for
pFN21ASDA1585
(Circular) (Six-base) MAPSORT of: pFN21ASDA1585.seq Check: 3704 from: 1 to: 6460
pFN21ASDA1585 6460 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2213 2213
Size: 6460
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4270 278
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3803 1937
Size: 1866 4594
Acc65I G'GTAC_C
Cuts at: 3781 3781
Size: 6460
AclI AA'CG_TT
Cuts at: 1563 4587 4960 1563
Size: 3024 373 3063
Fragments arranged by size:
3063 3024 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4523 5907 6175 1632
Size: 2891 1384 268 1917
Fragments arranged by size:
2891 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6263
AflIII A'CryG_T
Cuts at: 1203 5359 6336 1203
Size: 4156 977 1327
Fragments arranged by size:
4156 1327 977
AgeI A'CCGG_T
Cuts at: 5291 5291
Size: 6460
AhdI GACnn_n'nnGTC
Cuts at: 5189 5189
Size: 6460
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3644 1184
Size: 2460 4000
AlwNI CAG_nnn'CTG
Cuts at: 4203 5775 4203
Size: 1572 4888
ApaLI G'TGCA_C
Cuts at: 4517 5673 4517
Size: 1156 5304
ApoI r'AATT_y
Cuts at: 1493 3159 3769 3995 4147 6256 1493
Size: 1666 610 226 152 2109 1697
Fragments arranged by size:
2109 1697 1666 610 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5014 160
Size: 872 3982 1606
Fragments arranged by size:
3982 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
AvaI C'yCGr_G
Cuts at: 1870 1943 3785 1870
Size: 73 1842 4545
Fragments arranged by size:
4545 1842 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3625 3658 3625
Size: 33 6427
BamHI G'GATC_C
Cuts at: 3266 3790 4157 3266
Size: 524 367 5569
Fragments arranged by size:
5569 524 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3781 5236 618
Size: 325 217 627 1994 1455 1842
Fragments arranged by size:
1994 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3779 1391
Size: 2388 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5489
BbvCI CC'TCA_GC
Cuts at: 2567 2567
Size: 6460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4651 4685 1903
Size: 34 2714 34 3678
Fragments arranged by size:
3678 2714 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2926 4352 5562 2926
Size: 1426 1210 3824
Fragments arranged by size:
3824 1426 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6460
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5071 136
Size: 107 122 71 631 605 3399 1525
Fragments arranged by size:
3399 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6455 6455
Size: 6460
BlpI GC'TnA_GC
Cuts at: 3877 3877
Size: 6460
Bme1580I G_kGCm'C
Cuts at: 1790 4521 5677 1790
Size: 2731 1156 2573
Fragments arranged by size:
2731 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6460
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2941 3128 3671
Size: 416 53 83 399 24 1524 187 543
Cuts at: 3671 4191 5149 442
Size: 520 958 1753
Fragments arranged by size:
1753 1524 958 543 520 416 399 187
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2829 3572 5120 1239
Size: 1590 743 1548 2579
Fragments arranged by size:
2579 1590 1548 743
Bpu10I CC'TnA_GC
Cuts at: 2567 2567
Size: 6460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2517 4585 5450 5748 2517
Size: 2068 865 298 3229
Fragments arranged by size:
3229 2068 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2085 5123 882
Size: 617 586 3038 2219
Fragments arranged by size:
3038 2219 617 586
BsaAI yAC'GTr
Cuts at: 493 1204 3757 4251 493
Size: 711 2553 494 2702
Fragments arranged by size:
2702 2553 711 494
BsaBI GATnn'nnATC
Cuts at: 3052 6454 3052
Size: 3402 3058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4267 4649 5284 275
Size: 53 83 186 936 2734 382 635 1451
Fragments arranged by size:
2734 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4893 5291 5565 5712 6123 4893
Size: 398 274 147 411 5230
Fragments arranged by size:
5230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6235
Fragments arranged by size:
6235 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2459 1186
Size: 1273 5187
BseYI C'CCAG_C
Cuts at: 5663 6159 5663
Size: 496 5964
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2970 1163
Size: 573 1234 4653
Fragments arranged by size:
4653 1234 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3910 4671 5699 1540
Size: 275 186 1909 761 1028 2301
Fragments arranged by size:
2301 1909 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 4521 4606 5677 2076
Size: 2445 85 1071 2859
Fragments arranged by size:
2859 2445 1071 85
BsmI GAATG_Cn'
Cuts at: 2041 3971 4064 2041
Size: 1930 93 4437
Fragments arranged by size:
4437 1930 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 3779 4521 4606 5677 1391
Size: 399 286 1703 742 85 1071 2174
Fragments arranged by size:
2174 1703 1071 742 399 286 85
BspEI T'CCGG_A
Cuts at: 6123 6123
Size: 6460
BspHI T'CATG_A
Cuts at: 3256 4349 6079 3256
Size: 1093 1730 3637
Fragments arranged by size:
3637 1730 1093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2213 3815 844
Size: 1369 1602 3489
Fragments arranged by size:
3489 1602 1369
BsrBI CCG'CTC
Cuts at: 1394 1823 4347 1394
Size: 429 2524 3507
Fragments arranged by size:
3507 2524 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 3621 4955 5129 65
Size: 1183 1323 1050 1334 174 1396
Fragments arranged by size:
1396 1334 1323 1183 1050 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5104 5291 1700
Size: 110 3294 187 2869
Fragments arranged by size:
3294 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3005 3378 96
Size: 2909 373 3178
Fragments arranged by size:
3178 2909 373
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6460
BssSI C'ACGA_G
Cuts at: 4520 5532 4520
Size: 1012 5448
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3489 794
Size: 2695 3765
BstBI TT'CG_AA
Cuts at: 2007 5264 2007
Size: 3257 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6460
BstYI r'GATC_y
Cuts at: 1972 2179 2710 3266 3790 4157 4542 4559 6011
Size: 207 531 556 524 367 385 17 1452
Cuts at: 6011 6097 6109 6455 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 556 531 524 385 367 346
207 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5908
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5721
Fragments arranged by size:
5721 683 56
BtsI GCAGTG_nn'
Cuts at: 3976 4770 4790 3976
Size: 794 20 5646
Fragments arranged by size:
5646 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2755 2790 529
Size: 35 2191 35 4199
Fragments arranged by size:
4199 2191 35 35
DraI TTT'AAA
Cuts at: 3764 3764
Size: 6460
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5467 809
Size: 507 4151 1802
Fragments arranged by size:
4151 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3531 3907 4796
Size: 54 1425 50 275 18 1701 376 889
Cuts at: 4796 6425 8
Size: 1629 43
Fragments arranged by size:
1701 1629 1425 889 376 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3907 1537
Size: 275 2095 4090
Fragments arranged by size:
4090 2095 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3171 4390 1353
Size: 1818 1219 3423
Fragments arranged by size:
3423 1818 1219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5047 5421 5567 387
Size: 1064 138 3458 374 146 1280
Fragments arranged by size:
3458 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2829 3572 4523 5120 5907 6175 1239
Size: 393 1197 743 951 597 787 268 1524
Fragments arranged by size:
1524 1197 951 787 743 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3672 3672
Size: 6460
EcoRI G'AATT_C
Cuts at: 3769 4147 3769
Size: 378 6082
EcoRV GAT'ATC
Cuts at: 2779 2779
Size: 6460
FspI TGC'GCA
Cuts at: 4966 4966
Size: 6460
HaeII r_GCGC'y
Cuts at: 1678 5607 1678
Size: 3929 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2699 2731 3325 3357 5101
Size: 32 135 32 1127 32 594 32 1744
Cuts at: 5101 5133 5175 5207 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1744 1127 594 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2392 2695 3804 4050 669
Size: 988 281 454 303 1109 246 3079
Fragments arranged by size:
3079 1109 988 454 303 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6460
HpaI GTT'AAC
Cuts at: 4050 4050
Size: 6460
KpnI G_GTAC'C
Cuts at: 3785 3785
Size: 6460
MfeI C'AATT_G
Cuts at: 4059 4059
Size: 6460
MluI A'CGCG_T
Cuts at: 6336 6336
Size: 6460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2161 5574 5758 1305
Size: 856 3413 184 2007
Fragments arranged by size:
3413 2007 856 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3533 10
Size: 54 1425 343 1701 2937
Fragments arranged by size:
2937 1701 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 3261 3611 3626 4418 4777 4936
Size: 648 1152 943 350 15 792 359 159
Cuts at: 4936 518
Size: 2042
Fragments arranged by size:
2042 1152 943 792 648 359 350 159
15
MspA1I CmG'CkG
Cuts at: 1606 3876 4553 5701 5946 1606
Size: 2270 677 1148 245 2120
Fragments arranged by size:
2270 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5908
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6460
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6460
NotI GC'GGCC_GC
Cuts at: 3907 3907
Size: 6460
NspI r_CATG'y
Cuts at: 1132 1229 3450 3818 5363 1132
Size: 97 2221 368 1545 2229
Fragments arranged by size:
2229 2221 1545 368 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6460
PciI A'CATG_T
Cuts at: 5359 5359
Size: 6460
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6460
PmeI GTTT'AAAC
Cuts at: 3764 3764
Size: 6460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4529 4561 6042 6074 4529
Size: 32 1481 32 4915
Fragments arranged by size:
4915 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3672 3672
Size: 6460
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6460
PsiI TTA'TAA
Cuts at: 2613 4030 2613
Size: 1417 5043
PstI C_TGCA'G
Cuts at: 830 2786 2937 3812 830
Size: 1956 151 875 3478
Fragments arranged by size:
3478 1956 875 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6460
SalI G'TCGA_C
Cuts at: 1936 3802 1936
Size: 1866 4594
SanDI GG'GwC_CC
Cuts at: 3672 3672
Size: 6460
SbfI CC_TGCA'GG
Cuts at: 3812 3812
Size: 6460
ScaI AGT'ACT
Cuts at: 2841 4708 6420 2841
Size: 1867 1712 2881
Fragments arranged by size:
2881 1867 1712
SfcI C'TryA_G
Cuts at: 826 1045 2263 2782 2933 3174 3732 3808 4943
Size: 219 1218 519 151 241 558 76 1135
Cuts at: 4943 5624 5815 826
Size: 681 191 1471
Fragments arranged by size:
1471 1218 1135 681 558 519 241 219
191 151 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6460
SmaI CCC'GGG
Cuts at: 1872 3787 1872
Size: 1915 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2532 4564 5465 5727 820
Size: 197 926 589 2032 901 262 1553
Fragments arranged by size:
2032 1553 926 901 589 262 197
SnaBI TAC'GTA
Cuts at: 493 3757 4251 493
Size: 3264 494 2702
Fragments arranged by size:
3264 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6460
SphI G_CATG'C
Cuts at: 1132 3818 1132
Size: 2686 3774
SspI AAT'ATT
Cuts at: 5 52 2647 4384 5
Size: 47 2595 1737 2081
Fragments arranged by size:
2595 2081 1737 47
StuI AGG'CCT
Cuts at: 1517 3578 1517
Size: 2061 4399
StyI C'CwwG_G
Cuts at: 513 1065 2097 3579 3899 513
Size: 552 1032 1482 320 3074
Fragments arranged by size:
3074 1482 1032 552 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2206 4487 4504 4657 4842 5287 325
Size: 1255 626 2281 17 153 185 445 1498
Fragments arranged by size:
2281 1498 1255 626 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2839 3005 3223
Size: 274 80 33 51 1443 862 166 218
Cuts at: 3223 3378 3701 4706 6418 96
Size: 155 323 1005 1712 138
Fragments arranged by size:
1712 1443 1005 862 323 274 218 166
155 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2512 2544 6420 6452 2512
Size: 32 3876 32 2520
Fragments arranged by size:
3876 2520 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6460
XbaI T'CTAG_A
Cuts at: 3796 3796
Size: 6460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3598 3598
Size: 6460
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6460
XmaI C'CCGG_G
Cuts at: 1870 3785 1870
Size: 1915 4545
XmnI GAAnn'nnTTC
Cuts at: 3333 4589 3333
Size: 1256 5204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4268 276
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI