Restriction map for
pFN21ASDA1576
(Circular) (Six-base) MAPSORT of: pFN21ASDA1576.seq Check: 9392 from: 1 to: 5986
pFN21ASDA1576 5986 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2902 2902
Size: 5986
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2460 3329 1937
Size: 523 869 4594
Fragments arranged by size:
4594 869 523
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2182 2775 2872 4049 5433 5701 1632
Size: 550 593 97 1177 1384 268 1917
Fragments arranged by size:
1917 1384 1177 593 550 268 97
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 4885 5862 1203
Size: 3682 977 1327
Fragments arranged by size:
3682 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 2447 1166
Size: 1281 4705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 3729 5301 3729
Size: 1572 4414
ApaLI G'TGCA_C
Cuts at: 2579 4043 5199 2579
Size: 1464 1156 3366
Fragments arranged by size:
3366 1464 1156
ApoI r'AATT_y
Cuts at: 1493 3295 3521 3673 5782 1493
Size: 1802 226 152 2109 1697
Fragments arranged by size:
2109 1802 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2129 2213 3311 1870
Size: 73 186 84 1098 4545
Fragments arranged by size:
4545 1098 186 84 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2323 3307 4762 618
Size: 325 217 627 536 984 1455 1842
Fragments arranged by size:
1842 1455 984 627 536 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 2235 3045 3091 3305 1391
Size: 790 54 810 46 214 4072
Fragments arranged by size:
4072 810 790 214 54 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2807 928
Size: 971 908 4107
Fragments arranged by size:
4107 971 908
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2480 2514 4177 4211 1903
Size: 34 543 34 1663 34 3678
Fragments arranged by size:
3678 1663 543 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3006 4597 136
Size: 107 122 71 631 605 1334 1591 1525
Fragments arranged by size:
1591 1525 1334 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 2616 3403 2616
Size: 787 5199
Bme1580I G_kGCm'C
Cuts at: 1790 2583 4047 5203 1790
Size: 793 1464 1156 2573
Fragments arranged by size:
2573 1464 1156 793
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3717 4675 442
Size: 416 53 83 399 24 2300 958 1753
Fragments arranged by size:
2300 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2889 3113 4646 1239
Size: 1650 224 1533 2579
Fragments arranged by size:
2579 1650 1533 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 4111 4976 5274 2219
Size: 1892 865 298 2931
Fragments arranged by size:
2931 1892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2385 2813 2867 3197 4649 882
Size: 617 886 428 54 330 1452 2219
Fragments arranged by size:
2219 1452 886 617 428 330 54
BsaAI yAC'GTr
Cuts at: 493 1204 2728 3283 3777 493
Size: 711 1524 555 494 2702
Fragments arranged by size:
2702 1524 711 555 494
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 3793 4175 4810
Size: 53 83 186 936 603 1657 382 635
Cuts at: 4810 275
Size: 1451
Fragments arranged by size:
1657 1451 936 635 603 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4419 4817 5091 5238 5649 4419
Size: 398 274 147 411 4756
Fragments arranged by size:
4756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2382 2412 3160 3190 1178
Size: 30 165 30 979 30 748 30 3974
Fragments arranged by size:
3974 979 748 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2960 3110 3239 3242 1186
Size: 880 894 150 129 3 3930
Fragments arranged by size:
3930 894 880 150 129 3
BseYI C'CCAG_C
Cuts at: 5189 5685 5189
Size: 496 5490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2425 1163
Size: 573 689 4724
Fragments arranged by size:
4724 689 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3436 4197 5225 1540
Size: 275 186 1129 306 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 306 275 186
BsiHKAI G_wGCw'C
Cuts at: 2235 2577 2583 4047 4132 5203 2235
Size: 342 6 1464 85 1071 3018
Fragments arranged by size:
3018 1464 1071 342 85 6
BsmI GAATG_Cn'
Cuts at: 2501 3497 3590 2501
Size: 996 93 4897
Fragments arranged by size:
4897 996 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2048 2048
Size: 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2235 2577 2583 3045 3091 3305
Size: 399 391 54 342 6 462 46 214
Cuts at: 3305 4047 4132 5203 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 462 399 391 342 214
85 54 46 6
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2902 3341 844
Size: 2058 439 3489
Fragments arranged by size:
3489 2058 439
BsrBI CCG'CTC
Cuts at: 1394 1823 3262 3873 1394
Size: 429 1439 611 3507
Fragments arranged by size:
3507 1439 611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4481 4655 65
Size: 1183 3233 174 1396
Fragments arranged by size:
3233 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 4630 4817 1700
Size: 110 282 2538 187 2869
Fragments arranged by size:
2869 2538 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 2149 1925
Size: 224 5762
BssSI C'ACGA_G
Cuts at: 2576 3236 4046 5058 2576
Size: 660 810 1012 3504
Fragments arranged by size:
3504 1012 810 660
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 2007 4790 2007
Size: 2783 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2238 2466 3316 3683 4068 4085 5537 5623
Size: 266 228 850 367 385 17 1452 86
Cuts at: 5623 5635 5981 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 850 385 367 346 266 228
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2220 2220
Size: 5986
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4477
Fragments arranged by size:
4477 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2944 487
Size: 683 56 1718 3529
Fragments arranged by size:
3529 1718 683 56
BtsI GCAGTG_nn'
Cuts at: 3502 4296 4316 3502
Size: 794 20 5172
Fragments arranged by size:
5172 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2236 2271 529
Size: 35 1672 35 4244
Fragments arranged by size:
4244 1672 35 35
DraI TTT'AAA
Cuts at: 2979 3290 2979
Size: 311 5675
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 3186 3433
Size: 54 1425 50 275 18 195 1161 247
Cuts at: 3433 4322 5951 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1161 889 275 247 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2993 3216 3916 1353
Size: 848 792 223 700 3423
Fragments arranged by size:
3423 848 792 700 223
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 2111 4573 4947 5093 387
Size: 1064 138 474 48 2462 374 146 1280
Fragments arranged by size:
2462 1280 1064 474 374 146 138 48
EcoICRI GAG'CTC
Cuts at: 2233 2233
Size: 5986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2775 2872 2889 3113 4049 4646
Size: 393 550 593 97 17 224 936 597
Cuts at: 4646 5433 5701 1239
Size: 787 268 1524
Fragments arranged by size:
1524 936 787 597 593 550 393 268
224 97 17
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 5986
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 5133 1678
Size: 3455 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2382 2414 3160 3192 4627
Size: 32 135 32 810 32 746 32 1435
Cuts at: 4627 4659 4701 4733 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1435 810 746 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3330 3576 669
Size: 988 281 1392 246 3079
Fragments arranged by size:
3079 1392 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2880 5100 5284 1305
Size: 1575 2220 184 2007
Fragments arranged by size:
2220 2007 1575 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 3188 10
Size: 54 1425 343 195 1161 2808
Fragments arranged by size:
2808 1425 1161 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2447 3944 4303 4462 518
Size: 648 1281 1497 359 159 2042
Fragments arranged by size:
2042 1497 1281 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2588 2620 3118 3402 4079 5227 5472 1606
Size: 982 32 498 284 677 1148 245 2120
Fragments arranged by size:
2120 1148 982 677 498 284 245 32
NaeI GCC'GGC
Cuts at: 2094 2094
Size: 5986
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4477
Fragments arranged by size:
4477 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NgoMIV G'CCGG_C
Cuts at: 2092 2092
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 3344 4889 1132
Size: 97 2115 1545 2229
Fragments arranged by size:
2229 2115 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2827 1348
Size: 1479 4507
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5986
PfoI T'CCnGG_A
Cuts at: 2469 2556 2469
Size: 87 5899
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2647 2679 2647
Size: 32 5954
PstI C_TGCA'G
Cuts at: 830 3338 830
Size: 2508 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 2588 2620 2588
Size: 32 5954
SacI G_AGCT'C
Cuts at: 2235 2235
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SapI GCTCTTCn'nnn_
Cuts at: 2201 2993 2201
Size: 792 5194
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 3334 4469 5150 5341 826
Size: 219 2289 1135 681 191 1471
Fragments arranged by size:
2289 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3006 3006
Size: 5986
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 4090 4991 5253 820
Size: 197 926 291 1856 901 262 1553
Fragments arranged by size:
1856 1553 926 901 291 262 197
SnaBI TAC'GTA
Cuts at: 493 3283 3777 493
Size: 2790 494 2702
Fragments arranged by size:
2790 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 2296 3910 5
Size: 47 2244 1614 2081
Fragments arranged by size:
2244 2081 1614 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2022 2028 3425 513
Size: 552 957 6 1397 3074
Fragments arranged by size:
3074 1397 957 552 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3144 4013 4030 4183 4368 4813 325
Size: 1255 1564 869 17 153 185 445 1498
Fragments arranged by size:
1564 1498 1255 869 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4232 5944 96
Size: 274 80 33 51 1443 2255 1712 138
Fragments arranged by size:
2255 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3068 3100 5946 5978 3068
Size: 32 2846 32 3076
Fragments arranged by size:
3076 2846 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2396 1316
Size: 1080 4906
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2035 2317 2035
Size: 282 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 4115 4115
Size: 5986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI
SexAI SfoI SrfI SwaI