Restriction map for
pFN21ASDA1572
(Circular) (Six-base) MAPSORT of: pFN21ASDA1572.seq Check: 3316 from: 1 to: 6052
pFN21ASDA1572 6052 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2817 2923 3395 1937
Size: 880 106 472 4594
Fragments arranged by size:
4594 880 472 106
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 2550 4115 5499 5767 1632
Size: 704 214 1565 1384 268 1917
Fragments arranged by size:
1917 1565 1384 704 268 214
AflII C'TTAA_G
Cuts at: 820 1017 2194 3319 820
Size: 197 1177 1125 3553
Fragments arranged by size:
3553 1177 1125 197
AflIII A'CryG_T
Cuts at: 1203 4951 5928 1203
Size: 3748 977 1327
Fragments arranged by size:
3748 1327 977
AgeI A'CCGG_T
Cuts at: 4883 4883
Size: 6052
AhdI GACnn_n'nnGTC
Cuts at: 4781 4781
Size: 6052
AleI CACnn'nnGTG
Cuts at: 1166 2973 1166
Size: 1807 4245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2499 1184
Size: 1315 4737
AlwNI CAG_nnn'CTG
Cuts at: 2389 3283 3795 5367 2389
Size: 894 512 1572 3074
Fragments arranged by size:
3074 1572 894 512
ApaLI G'TGCA_C
Cuts at: 2377 4109 5265 2377
Size: 1732 1156 3164
Fragments arranged by size:
3164 1732 1156
ApoI r'AATT_y
Cuts at: 1493 2114 2303 2686 3361 3587 3739 5848 1493
Size: 621 189 383 675 226 152 2109 1697
Fragments arranged by size:
2109 1697 675 621 383 226 189 152
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 3377 1870
Size: 73 1434 4545
Fragments arranged by size:
4545 1434 73
BamHI G'GATC_C
Cuts at: 3382 3749 3382
Size: 367 5685
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3373 4828 618
Size: 325 217 627 1586 1455 1842
Fragments arranged by size:
1842 1586 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2670 3371 1391
Size: 1279 701 4072
Fragments arranged by size:
4072 1279 701
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2538 928
Size: 971 639 4442
Fragments arranged by size:
4442 971 639
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4243 4277 1903
Size: 34 2306 34 3678
Fragments arranged by size:
3678 2306 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3944 5154 3944
Size: 1210 4842
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4663 136
Size: 107 122 71 631 605 2991 1525
Fragments arranged by size:
2991 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2038 6047 2038
Size: 4009 2043
BlpI GC'TnA_GC
Cuts at: 3469 3469
Size: 6052
Bme1580I G_kGCm'C
Cuts at: 1790 2381 2849 4113 5269 1790
Size: 591 468 1264 1156 2573
Fragments arranged by size:
2573 1264 1156 591 468
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2455 3783 4741
Size: 416 53 83 399 24 1038 1328 958
Cuts at: 4741 442
Size: 1753
Fragments arranged by size:
1753 1328 1038 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6052
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2472 2472
Size: 6052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2897 4712 1239
Size: 1658 1815 2579
Fragments arranged by size:
2579 1815 1658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4177 5042 5340 4177
Size: 865 298 4889
Fragments arranged by size:
4889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2193 4715 882
Size: 617 694 2522 2219
Fragments arranged by size:
2522 2219 694 617
BsaAI yAC'GTr
Cuts at: 493 1204 3349 3843 493
Size: 711 2145 494 2702
Fragments arranged by size:
2702 2145 711 494
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3859 4241 4876 275
Size: 53 83 186 936 2326 382 635 1451
Fragments arranged by size:
2326 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4485 4883 5157 5304 5715 4485
Size: 398 274 147 411 4822
Fragments arranged by size:
4822 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5827
Fragments arranged by size:
5827 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6052
BseYI C'CCAG_C
Cuts at: 2670 5255 5751 2670
Size: 2585 496 2971
Fragments arranged by size:
2971 2585 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5479
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2381 4113 4198 5269 2381
Size: 1732 85 1071 3164
Fragments arranged by size:
3164 1732 1071 85
BsmI GAATG_Cn'
Cuts at: 3563 3656 3563
Size: 93 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2381 2670 2849 3371 4113 4198 5269
Size: 399 591 289 179 522 742 85 1071
Cuts at: 5269 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 591 522 399 289 179
85
BspEI T'CCGG_A
Cuts at: 5715 5715
Size: 6052
BspHI T'CATG_A
Cuts at: 3941 5671 3941
Size: 1730 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2171 3281 3407 844
Size: 1327 1110 126 3489
Fragments arranged by size:
3489 1327 1110 126
BsrBI CCG'CTC
Cuts at: 1394 1823 3939 1394
Size: 429 2116 3507
Fragments arranged by size:
3507 2116 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4547 4721 65
Size: 1183 3299 174 1396
Fragments arranged by size:
3299 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4696 4883 1700
Size: 110 2886 187 2869
Fragments arranged by size:
2886 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3190 3227 96
Size: 3094 37 2921
Fragments arranged by size:
3094 2921 37
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 4112 5124 4112
Size: 1012 5040
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6052
BstBI TT'CG_AA
Cuts at: 2007 4856 2007
Size: 2849 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6052
BstYI r'GATC_y
Cuts at: 1972 2038 3382 3749 4134 4151 5603 5689 5701
Size: 66 1344 367 385 17 1452 86 12
Cuts at: 5701 6047 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1344 385 367 346 86 66
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5500
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5313
Fragments arranged by size:
5313 683 56
BtsI GCAGTG_nn'
Cuts at: 2646 2767 3568 4362 4382 2646
Size: 121 801 794 20 4316
Fragments arranged by size:
4316 801 794 121 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 2898 3356 2898
Size: 458 5594
DraIII CAC_nnn'GTG
Cuts at: 2450 2450
Size: 6052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5059 809
Size: 507 3743 1802
Fragments arranged by size:
3743 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3499 4388 6017
Size: 54 1425 50 275 18 1669 889 1629
Cuts at: 6017 8
Size: 43
Fragments arranged by size:
1669 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 3982 1353
Size: 747 1882 3423
Fragments arranged by size:
3423 1882 747
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4639 5013 5159 387
Size: 1064 138 3050 374 146 1280
Fragments arranged by size:
3050 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2550 2897 4115 4712 5499 5767
Size: 393 704 214 347 1218 597 787 268
Cuts at: 5767 1239
Size: 1524
Fragments arranged by size:
1524 1218 787 704 597 393 347 268
214
EcoO109I rG'GnC_Cy
Cuts at: 2386 3280 2386
Size: 894 5158
EcoRI G'AATT_C
Cuts at: 2303 3361 3739 2303
Size: 1058 378 4616
Fragments arranged by size:
4616 1058 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2975 3058 2975
Size: 83 5969
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 5199 1678
Size: 3521 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 2259 2291 2929
Size: 32 135 32 453 32 202 32 638
Cuts at: 2929 2961 4693 4725 4767 4799 1373
Size: 32 1732 32 42 32 2626
Fragments arranged by size:
2626 1732 638 453 202 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3396 3642 669
Size: 988 281 1458 246 3079
Fragments arranged by size:
3079 1458 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6052
HpaI GTT'AAC
Cuts at: 3642 3642
Size: 6052
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 2270 3651 2270
Size: 1381 4671
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5166 5350 1305
Size: 3861 184 2007
Fragments arranged by size:
3861 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4230
Fragments arranged by size:
4230 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2739 2774 2973 2975 4010 4369 4528
Size: 648 1573 35 199 2 1035 359 159
Cuts at: 4528 518
Size: 2042
Fragments arranged by size:
2042 1573 1035 648 359 199 159 35
2
MspA1I CmG'CkG
Cuts at: 1606 3148 3468 4145 5293 5538 1606
Size: 1542 320 677 1148 245 2120
Fragments arranged by size:
2120 1542 1148 677 320 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5500
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6052
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 2974 2984 3228 3410 4955 1132
Size: 97 1745 10 244 182 1545 2229
Fragments arranged by size:
2229 1745 1545 244 182 97 10
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6052
PciI A'CATG_T
Cuts at: 4951 4951
Size: 6052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6052
PfoI T'CCnGG_A
Cuts at: 3282 3282
Size: 6052
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 5634 5666 4121
Size: 32 1481 32 4507
Fragments arranged by size:
4507 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3280 3280
Size: 6052
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 2402 3622 2402
Size: 1220 4832
PstI C_TGCA'G
Cuts at: 830 2182 3404 830
Size: 1352 1222 3478
Fragments arranged by size:
3478 1352 1222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
PvuII CAG'CTG
Cuts at: 3148 3148
Size: 6052
SalI G'TCGA_C
Cuts at: 1936 3394 1936
Size: 1458 4594
SbfI CC_TGCA'GG
Cuts at: 3404 3404
Size: 6052
ScaI AGT'ACT
Cuts at: 3334 4300 6012 3334
Size: 966 1712 3374
Fragments arranged by size:
3374 1712 966
SfcI C'TryA_G
Cuts at: 826 1045 2178 2828 3400 4535 5216 5407 826
Size: 219 1133 650 572 1135 681 191 1471
Fragments arranged by size:
1471 1135 1133 681 650 572 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6052
SmaI CCC'GGG
Cuts at: 1872 3379 1872
Size: 1507 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2194 3319 4156 5057 5319 820
Size: 197 926 251 1125 837 901 262 1553
Fragments arranged by size:
1553 1125 926 901 837 262 251 197
SnaBI TAC'GTA
Cuts at: 493 3349 3843 493
Size: 2856 494 2702
Fragments arranged by size:
2856 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 2984 3410 1132
Size: 1852 426 3774
Fragments arranged by size:
3774 1852 426
SspI AAT'ATT
Cuts at: 5 52 2806 3976 5
Size: 47 2754 1170 2081
Fragments arranged by size:
2754 2081 1170 47
StuI AGG'CCT
Cuts at: 1517 2826 1517
Size: 1309 4743
StyI C'CwwG_G
Cuts at: 513 1065 3491 513
Size: 552 2426 3074
Fragments arranged by size:
3074 2426 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4079 4096 4249 4434 4879 325
Size: 1255 2499 17 153 185 445 1498
Fragments arranged by size:
2499 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3190 3227 3332
Size: 274 80 33 51 1443 1213 37 105
Cuts at: 3332 4298 6010 96
Size: 966 1712 138
Fragments arranged by size:
1712 1443 1213 966 274 138 105 80
51 37 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6012 6044 6012
Size: 32 6020
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6052
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6052
XmaI C'CCGG_G
Cuts at: 1870 3377 1870
Size: 1507 4545
XmnI GAAnn'nnTTC
Cuts at: 4181 4181
Size: 6052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI