Restriction map for
pFN21ASDA1562
(Circular) (Six-base) MAPSORT of: pFN21ASDA1562.seq Check: 4983 from: 1 to: 8134
pFN21ASDA1562 8134 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5944 278
Size: 53 83 186 936 4408 2468
Fragments arranged by size:
4408 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5477 1937
Size: 3540 4594
Acc65I G'GTAC_C
Cuts at: 5455 5455
Size: 8134
AclI AA'CG_TT
Cuts at: 1563 3738 3945 6261 6634 1563
Size: 2175 207 2316 373 3063
Fragments arranged by size:
3063 2316 2175 373 207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2648 2666 2860 3227 4058 4074 6197 7581
Size: 1016 18 194 367 831 16 2123 1384
Cuts at: 7581 7849 1632
Size: 268 1917
Fragments arranged by size:
2123 1917 1384 1016 831 367 268 194
18 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7937
AflIII A'CryG_T
Cuts at: 1203 4860 7033 8010 1203
Size: 3657 2173 977 1327
Fragments arranged by size:
3657 2173 1327 977
AgeI A'CCGG_T
Cuts at: 6965 6965
Size: 8134
AhdI GACnn_n'nnGTC
Cuts at: 3351 6863 3351
Size: 3512 4622
AleI CACnn'nnGTG
Cuts at: 1166 3650 3707 4304 5253 1166
Size: 2484 57 597 949 4047
Fragments arranged by size:
4047 2484 949 597 57
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8134
AlwNI CAG_nnn'CTG
Cuts at: 3497 5877 7449 3497
Size: 2380 1572 4182
Fragments arranged by size:
4182 2380 1572
ApaLI G'TGCA_C
Cuts at: 6191 7347 6191
Size: 1156 6978
ApoI r'AATT_y
Cuts at: 1493 2107 2239 2527 3016 3434 4468 4622 4752
Size: 614 132 288 489 418 1034 154 130
Cuts at: 4752 4880 5443 5669 5821 7930 1493
Size: 128 563 226 152 2109 1697
Fragments arranged by size:
2109 1697 1034 614 563 489 418 288
226 154 152 132 130 128
AseI AT'TA_AT
Cuts at: 160 1032 2412 6688 160
Size: 872 1380 4276 1606
Fragments arranged by size:
4276 1606 1380 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8134
AvaI C'yCGr_G
Cuts at: 1870 1943 5459 1870
Size: 73 3516 4545
Fragments arranged by size:
4545 3516 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3141 3174 3141
Size: 33 8101
BamHI G'GATC_C
Cuts at: 2836 3139 5464 5831 2836
Size: 303 2325 367 5139
Fragments arranged by size:
5139 2325 367 303
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4306 5455 6910 618
Size: 325 217 627 2519 1149 1455 1842
Fragments arranged by size:
2519 1842 1455 1149 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5453 1391
Size: 4062 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3254 4833 928
Size: 971 1355 1579 4229
Fragments arranged by size:
4229 1579 1355 971
BbvCI CC'TCA_GC
Cuts at: 3333 4014 3333
Size: 681 7453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6325 6359 1903
Size: 34 4388 34 3678
Fragments arranged by size:
4388 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5008 6026 7236 5008
Size: 1018 1210 5906
Fragments arranged by size:
5906 1210 1018
BclI T'GATC_A
Cuts at: 1548 2352 3622 1548
Size: 804 1270 6060
Fragments arranged by size:
6060 1270 804
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4276 6745 136
Size: 107 122 71 631 605 2604 2469 1525
Fragments arranged by size:
2604 2469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2438 2821 3305 4337 8129 2438
Size: 383 484 1032 3792 2443
Fragments arranged by size:
3792 2443 1032 484 383
BlpI GC'TnA_GC
Cuts at: 4935 5551 4935
Size: 616 7518
Bme1580I G_kGCm'C
Cuts at: 1790 6195 7351 1790
Size: 4405 1156 2573
Fragments arranged by size:
4405 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8134
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2108 2422 3345
Size: 416 53 83 399 24 691 314 923
Cuts at: 3345 5019 5865 6823 442
Size: 1674 846 958 1753
Fragments arranged by size:
1753 1674 958 923 846 691 416 399
314 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4489 4531 4489
Size: 42 8092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5147 5236 6794 1239
Size: 3908 89 1558 2579
Fragments arranged by size:
3908 2579 1558 89
Bpu10I CC'TnA_GC
Cuts at: 3333 4014 3333
Size: 681 7453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2985 4943 6259 7124 7422 2985
Size: 1958 1316 865 298 3697
Fragments arranged by size:
3697 1958 1316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4519 6797 882
Size: 617 3020 2278 2219
Fragments arranged by size:
3020 2278 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4459 5431 5925 493
Size: 711 3255 972 494 2702
Fragments arranged by size:
3255 2702 972 711 494
BsaBI GATnn'nnATC
Cuts at: 3498 8128 3498
Size: 4630 3504
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5941 6323 6958 275
Size: 53 83 186 936 4408 382 635 1451
Fragments arranged by size:
4408 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4949 6567 6965 7239 7386 7797 4949
Size: 1618 398 274 147 411 5286
Fragments arranged by size:
5286 1618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2633 2663 3192 3222 1178
Size: 30 165 30 1230 30 529 30 6090
Fragments arranged by size:
6090 1230 529 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2235 3911 4796 5306 1186
Size: 1049 1676 885 510 4014
Fragments arranged by size:
4014 1676 1049 885 510
BseYI C'CCAG_C
Cuts at: 3025 4896 7337 7833 3025
Size: 1871 2441 496 3326
Fragments arranged by size:
3326 2441 1871 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2490 4173 4759 4807 5279 1163
Size: 573 754 1683 586 48 472 4018
Fragments arranged by size:
4018 1683 754 586 573 472 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5584 6345 7373 1540
Size: 275 186 3583 761 1028 2301
Fragments arranged by size:
3583 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3714 5337 6195 6280 7351 3714
Size: 1623 858 85 1071 4497
Fragments arranged by size:
4497 1623 1071 858 85
BsmI GAATG_Cn'
Cuts at: 2041 2487 2833 3865 5102 5645 5738 2041
Size: 446 346 1032 1237 543 93 4437
Fragments arranged by size:
4437 1237 1032 543 446 346 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4359 5069 4359
Size: 710 7424
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3714 5337 5453 6195 6280 7351 1391
Size: 399 1924 1623 116 742 85 1071 2174
Fragments arranged by size:
2174 1924 1623 1071 742 399 116 85
BspEI T'CCGG_A
Cuts at: 7797 7797
Size: 8134
BspHI T'CATG_A
Cuts at: 3625 6023 7753 3625
Size: 2398 1730 4006
Fragments arranged by size:
4006 2398 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3878 4501 5489 844
Size: 3034 623 988 3489
Fragments arranged by size:
3489 3034 988 623
BsrBI CCG'CTC
Cuts at: 1394 1823 6021 1394
Size: 429 4198 3507
Fragments arranged by size:
4198 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3031 3761 4089 6629 6803 65
Size: 1183 1783 730 328 2540 174 1396
Fragments arranged by size:
2540 1783 1396 1183 730 328 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4398 6778 6965 1700
Size: 110 2588 2380 187 2869
Fragments arranged by size:
2869 2588 2380 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8134
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8134
BssSI C'ACGA_G
Cuts at: 6194 7206 6194
Size: 1012 7122
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4151 794
Size: 3357 4777
BstBI TT'CG_AA
Cuts at: 2007 6938 2007
Size: 4931 3203
BstEII G'GTnAC_C
Cuts at: 1184 5165 1184
Size: 3981 4153
BstXI CCAn_nnnn'nTGG
Cuts at: 2902 5125 5161 5383 2902
Size: 2223 36 222 5653
Fragments arranged by size:
5653 2223 222 36
BstYI r'GATC_y
Cuts at: 1972 2438 2821 2836 3139 3305 3493 4217 4337
Size: 466 383 15 303 166 188 724 120
Cuts at: 4337 5295 5464 5831 6216 6233 7685 7771 7783
Size: 958 169 367 385 17 1452 86 12
Cuts at: 7783 8129 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 958 724 466 385 383 367
346 303 188 169 166 120 86 17
15 12
Bsu36I CC'TnA_GG
Cuts at: 4138 4138
Size: 8134
BtgI C'CryG_G
Cuts at: 513 1065 2832 4645 5286 513
Size: 552 1767 1813 641 3361
Fragments arranged by size:
3361 1813 1767 641 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7395
Fragments arranged by size:
7395 683 56
BtsI GCAGTG_nn'
Cuts at: 2670 4161 4826 5056 5107 5650 6444 6464 2670
Size: 1491 665 230 51 543 794 20 4340
Fragments arranged by size:
4340 1491 794 665 543 230 51 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8099
DraI TTT'AAA
Cuts at: 2914 3238 5438 2914
Size: 324 2200 5610
Fragments arranged by size:
5610 2200 324
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7141 809
Size: 507 5825 1802
Fragments arranged by size:
5825 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4236 4642 5581
Size: 54 1425 50 275 18 2406 406 939
Cuts at: 5581 6470 8099 8
Size: 889 1629 43
Fragments arranged by size:
2406 1629 1425 939 889 406 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5581 1537
Size: 275 3769 4090
Fragments arranged by size:
4090 3769 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4958 6064 1353
Size: 3605 1106 3423
Fragments arranged by size:
3605 3423 1106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5408 6721 7095 7241 387
Size: 1064 138 3819 1313 374 146 1280
Fragments arranged by size:
3819 1313 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2648 2666 2860 3227 4058 4074 5147
Size: 393 1016 18 194 367 831 16 1073
Cuts at: 5147 5236 6197 6794 7581 7849 1239
Size: 89 961 597 787 268 1524
Fragments arranged by size:
1524 1073 1016 961 831 787 597 393
367 268 194 89 18 16
EcoO109I rG'GnC_Cy
Cuts at: 3755 4792 5141 3755
Size: 1037 349 6748
Fragments arranged by size:
6748 1037 349
EcoRI G'AATT_C
Cuts at: 4752 4880 5443 5821 4752
Size: 128 563 378 7065
Fragments arranged by size:
7065 563 378 128
EcoRV GAT'ATC
Cuts at: 2460 3394 2460
Size: 934 7200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5161 5161
Size: 8134
FspI TGC'GCA
Cuts at: 6640 6640
Size: 8134
HaeII r_GCGC'y
Cuts at: 1678 2853 7281 1678
Size: 1175 4428 2531
Fragments arranged by size:
4428 2531 1175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2633 2665 2753 2785 3337
Size: 32 135 32 1061 32 88 32 552
Cuts at: 3337 3369 3833 3865 3908 3940 4202 4234 6775
Size: 32 464 32 43 32 262 32 2541
Cuts at: 6775 6807 6849 6881 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2541 1061 552 464 262 135 88
43 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5478 5724 669
Size: 988 281 3540 246 3079
Fragments arranged by size:
3540 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2192 3215 748
Size: 1444 1023 5667
Fragments arranged by size:
5667 1444 1023
HpaI GTT'AAC
Cuts at: 5724 5724
Size: 8134
KpnI G_GTAC'C
Cuts at: 5459 5459
Size: 8134
MfeI C'AATT_G
Cuts at: 2295 3837 5733 2295
Size: 1542 1896 4696
Fragments arranged by size:
4696 1896 1542
MluI A'CGCG_T
Cuts at: 8010 8010
Size: 8134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2122 5142 7248 7432 1305
Size: 817 3020 2106 184 2007
Fragments arranged by size:
3020 2106 2007 817 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6312
Fragments arranged by size:
6312 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3225 3650 3707 3925 4304 5253 5346
Size: 648 2059 425 57 218 379 949 93
Cuts at: 5346 6092 6451 6610 518
Size: 746 359 159 2042
Fragments arranged by size:
2059 2042 949 746 648 425 379 359
218 159 93 57
MspA1I CmG'CkG
Cuts at: 1606 3873 5550 6227 7375 7620 1606
Size: 2267 1677 677 1148 245 2120
Fragments arranged by size:
2267 2120 1677 1148 677 245
NaeI GCC'GGC
Cuts at: 4400 4400
Size: 8134
NcoI C'CATG_G
Cuts at: 513 1065 5286 513
Size: 552 4221 3361
Fragments arranged by size:
4221 3361 552
NdeI CA'TA_TG
Cuts at: 387 3253 3571 387
Size: 2866 318 4950
Fragments arranged by size:
4950 2866 318
NgoMIV G'CCGG_C
Cuts at: 4398 4398
Size: 8134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8134
NotI GC'GGCC_GC
Cuts at: 5581 5581
Size: 8134
NspI r_CATG'y
Cuts at: 1132 1229 4864 5492 7037 1132
Size: 97 3635 628 1545 2229
Fragments arranged by size:
3635 2229 1545 628 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8134
PciI A'CATG_T
Cuts at: 4860 7033 4860
Size: 2173 5961
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2519 3593 1108
Size: 1411 1074 5649
Fragments arranged by size:
5649 1411 1074
PfoI T'CCnGG_A
Cuts at: 4586 4586
Size: 8134
PmeI GTTT'AAAC
Cuts at: 5438 5438
Size: 8134
PmlI CAC'GTG
Cuts at: 4459 4459
Size: 8134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6203 6235 7716 7748 6203
Size: 32 1481 32 6589
Fragments arranged by size:
6589 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4792 5141 4792
Size: 349 7785
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8134
PsiI TTA'TAA
Cuts at: 3044 5704 3044
Size: 2660 5474
PstI C_TGCA'G
Cuts at: 830 2379 4232 4945 5486 830
Size: 1549 1853 713 541 3478
Fragments arranged by size:
3478 1853 1549 713 541
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8134
PvuII CAG'CTG
Cuts at: 3873 3873
Size: 8134
SalI G'TCGA_C
Cuts at: 1936 5476 1936
Size: 3540 4594
SanDI GG'GwC_CC
Cuts at: 5141 5141
Size: 8134
SbfI CC_TGCA'GG
Cuts at: 5486 5486
Size: 8134
ScaI AGT'ACT
Cuts at: 6382 8094 6382
Size: 1712 6422
SfcI C'TryA_G
Cuts at: 826 1045 2375 3360 4228 4594 4941 5482 6617
Size: 219 1330 985 868 366 347 541 1135
Cuts at: 6617 7298 7489 826
Size: 681 191 1471
Fragments arranged by size:
1471 1330 1135 985 868 681 541 366
347 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8134
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8134
SmaI CCC'GGG
Cuts at: 1872 5461 1872
Size: 3589 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3000 4922 6238 7139 7401 820
Size: 197 926 1057 1922 1316 901 262 1553
Fragments arranged by size:
1922 1553 1316 1057 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5431 5925 493
Size: 4938 494 2702
Fragments arranged by size:
4938 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8134
SphI G_CATG'C
Cuts at: 1132 5492 1132
Size: 4360 3774
SspI AAT'ATT
Cuts at: 5 52 4583 6058 5
Size: 47 4531 1475 2081
Fragments arranged by size:
4531 2081 1475 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8134
StyI C'CwwG_G
Cuts at: 513 1065 4107 5286 5573 513
Size: 552 3042 1179 287 3074
Fragments arranged by size:
3074 3042 1179 552 287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3364 4275 4502 4703 5103 6161 6178
Size: 1255 1784 911 227 201 400 1058 17
Cuts at: 6178 6331 6516 6961 325
Size: 153 185 445 1498
Fragments arranged by size:
1784 1498 1255 1058 911 445 400 227
201 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2095 4076 6380
Size: 274 80 33 51 1443 118 1981 2304
Cuts at: 6380 8092 96
Size: 1712 138
Fragments arranged by size:
2304 1981 1712 1443 274 138 118 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3399 3431 8094 8126 3399
Size: 32 4663 32 3407
Fragments arranged by size:
4663 3407 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3228 1316
Size: 1912 6222
XbaI T'CTAG_A
Cuts at: 5470 5470
Size: 8134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3949 5348 5383 3949
Size: 1399 35 6700
Fragments arranged by size:
6700 1399 35
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8134
XmaI C'CCGG_G
Cuts at: 1870 5459 1870
Size: 3589 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 6263 3905
Size: 2358 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5942 276
Size: 53 83 186 936 4408 2468
Fragments arranged by size:
4408 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI