Restriction map for
pFN21ASDA1559
(Circular) (Six-base) MAPSORT of: pFN21ASDA1559.seq Check: 6587 from: 1 to: 6328
pFN21ASDA1559 6328 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4138 278
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3671 1937
Size: 1734 4594
Acc65I G'GTAC_C
Cuts at: 3649 3649
Size: 6328
AclI AA'CG_TT
Cuts at: 1563 4455 4828 1563
Size: 2892 373 3063
Fragments arranged by size:
3063 2892 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 4391 5775 6043 1632
Size: 716 2043 1384 268 1917
Fragments arranged by size:
2043 1917 1384 716 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6131
AflIII A'CryG_T
Cuts at: 1203 5227 6204 1203
Size: 4024 977 1327
Fragments arranged by size:
4024 1327 977
AgeI A'CCGG_T
Cuts at: 2690 5159 2690
Size: 2469 3859
AhdI GACnn_n'nnGTC
Cuts at: 5057 5057
Size: 6328
AleI CACnn'nnGTG
Cuts at: 1166 2691 2859 3363 3447 1166
Size: 1525 168 504 84 4047
Fragments arranged by size:
4047 1525 504 168 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6328
AlwNI CAG_nnn'CTG
Cuts at: 2833 4071 5643 2833
Size: 1238 1572 3518
Fragments arranged by size:
3518 1572 1238
ApaLI G'TGCA_C
Cuts at: 2658 4385 5541 2658
Size: 1727 1156 3445
Fragments arranged by size:
3445 1727 1156
ApoI r'AATT_y
Cuts at: 1493 2084 2598 2850 3018 3522 3618 3637 3863
Size: 591 514 252 168 504 96 19 226
Cuts at: 3863 4015 6124 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 591 514 504 252 226 168
152 96 19
AseI AT'TA_AT
Cuts at: 160 1032 4882 160
Size: 872 3850 1606
Fragments arranged by size:
3850 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 3653 1870
Size: 73 1710 4545
Fragments arranged by size:
4545 1710 73
BamHI G'GATC_C
Cuts at: 2092 3658 4025 2092
Size: 1566 367 4395
Fragments arranged by size:
4395 1566 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3649 5104 618
Size: 325 217 627 1862 1455 1842
Fragments arranged by size:
1862 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3647 1391
Size: 2256 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2564 3152 3236 928
Size: 971 665 588 84 4020
Fragments arranged by size:
4020 971 665 588 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2819 2853 4519 4553 1903
Size: 34 882 34 1666 34 3678
Fragments arranged by size:
3678 1666 882 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4220 5430 4220
Size: 1210 5118
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6328
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4939 136
Size: 107 122 71 631 605 3267 1525
Fragments arranged by size:
3267 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6323 6323
Size: 6328
BlpI GC'TnA_GC
Cuts at: 3745 3745
Size: 6328
Bme1580I G_kGCm'C
Cuts at: 1790 2662 4389 5545 1790
Size: 872 1727 1156 2573
Fragments arranged by size:
2573 1727 1156 872
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4059 5017 442
Size: 416 53 83 399 24 2642 958 1753
Fragments arranged by size:
2642 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2277 4988 1239
Size: 1038 2711 2579
Fragments arranged by size:
2711 2579 1038
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4453 5318 5616 4453
Size: 865 298 5165
Fragments arranged by size:
5165 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3280 4991 882
Size: 617 1781 1711 2219
Fragments arranged by size:
2219 1781 1711 617
BsaAI yAC'GTr
Cuts at: 493 1204 3625 4119 493
Size: 711 2421 494 2702
Fragments arranged by size:
2702 2421 711 494
BsaBI GATnn'nnATC
Cuts at: 6322 6322
Size: 6328
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4135 4517 5152 275
Size: 53 83 186 936 2602 382 635 1451
Fragments arranged by size:
2602 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2690 4761 5159 5433 5580 5991 2690
Size: 2071 398 274 147 411 3027
Fragments arranged by size:
3027 2071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2275 2305 2517 2547 1178
Size: 30 165 30 872 30 212 30 4959
Fragments arranged by size:
4959 872 212 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6328
BseYI C'CCAG_C
Cuts at: 5531 6027 5531
Size: 496 5832
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5755
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3778 4539 5567 1540
Size: 275 186 1777 761 1028 2301
Fragments arranged by size:
2301 1777 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2662 2742 4389 4474 5545 2662
Size: 80 1647 85 1071 3445
Fragments arranged by size:
3445 1647 1071 85 80
BsmI GAATG_Cn'
Cuts at: 2399 3270 3580 3839 3932 2399
Size: 871 310 259 93 4795
Fragments arranged by size:
4795 871 310 259 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2662 2742 3647 4389 4474 5545 1391
Size: 399 872 80 905 742 85 1071 2174
Fragments arranged by size:
2174 1071 905 872 742 399 85 80
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 4217 5947 4217
Size: 1730 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3683 844
Size: 2839 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4215 1394
Size: 429 2392 3507
Fragments arranged by size:
3507 2392 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4823 4997 65
Size: 1183 3575 174 1396
Fragments arranged by size:
3575 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2690 4972 5159 1700
Size: 110 880 2282 187 2869
Fragments arranged by size:
2869 2282 880 187 110
BsrGI T'GTAC_A
Cuts at: 96 2909 96
Size: 2813 3515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6328
BssSI C'ACGA_G
Cuts at: 3195 4388 5400 3195
Size: 1193 1012 4123
Fragments arranged by size:
4123 1193 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6328
BstBI TT'CG_AA
Cuts at: 2007 5132 2007
Size: 3125 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6328
BstYI r'GATC_y
Cuts at: 1972 2092 3658 4025 4410 4427 5879 5965 5977
Size: 120 1566 367 385 17 1452 86 12
Cuts at: 5977 6323 1972
Size: 346 1977
Fragments arranged by size:
1977 1566 1452 385 367 346 120 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2029 513
Size: 552 957 7 4812
Fragments arranged by size:
4812 957 552 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5589
Fragments arranged by size:
5589 683 56
BtsI GCAGTG_nn'
Cuts at: 3844 4638 4658 3844
Size: 794 20 5514
Fragments arranged by size:
5514 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6293
DraI TTT'AAA
Cuts at: 3632 3632
Size: 6328
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5335 809
Size: 507 4019 1802
Fragments arranged by size:
4019 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 3775 4664
Size: 54 1425 50 275 18 225 1720 889
Cuts at: 4664 6293 8
Size: 1629 43
Fragments arranged by size:
1720 1629 1425 889 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3775 1537
Size: 275 1963 4090
Fragments arranged by size:
4090 1963 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3297 4258 1353
Size: 1944 961 3423
Fragments arranged by size:
3423 1944 961
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4915 5289 5435 387
Size: 1064 138 3326 374 146 1280
Fragments arranged by size:
3326 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2277 2348 4391 4988 5775 6043 1239
Size: 393 645 71 2043 597 787 268 1524
Fragments arranged by size:
2043 1524 787 645 597 393 268 71
EcoO109I rG'GnC_Cy
Cuts at: 2159 2159
Size: 6328
EcoRI G'AATT_C
Cuts at: 2084 2598 2850 3522 3637 4015 2084
Size: 514 252 672 115 378 4397
Fragments arranged by size:
4397 672 514 378 252 115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3586 3586
Size: 6328
FspI TGC'GCA
Cuts at: 4834 4834
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 5475 1678
Size: 3797 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 4969 5001 5043
Size: 32 135 32 677 32 2688 32 42
Cuts at: 5043 5075 1373
Size: 32 2626
Fragments arranged by size:
2688 2626 677 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2588 3672 3918 669
Size: 988 281 650 1084 246 3079
Fragments arranged by size:
3079 1084 988 650 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6328
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KpnI G_GTAC'C
Cuts at: 3653 3653
Size: 6328
MfeI C'AATT_G
Cuts at: 3927 3927
Size: 6328
MluI A'CGCG_T
Cuts at: 6204 6204
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3206 5442 5626 1305
Size: 1901 2236 184 2007
Fragments arranged by size:
2236 2007 1901 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 10
Size: 54 1425 343 225 4281
Fragments arranged by size:
4281 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2326 2607 2691 2775 2859 3027 3111
Size: 648 1160 281 84 84 84 168 84
Cuts at: 3111 3195 3279 3363 3447 3531 4286 4645 4804
Size: 84 84 84 84 84 755 359 159
Cuts at: 4804 518
Size: 2042
Fragments arranged by size:
2042 1160 755 648 359 281 168 159
84 84 84 84 84 84 84 84
84
MspA1I CmG'CkG
Cuts at: 1606 3744 4421 5569 5814 1606
Size: 2138 677 1148 245 2120
Fragments arranged by size:
2138 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2029 513
Size: 552 957 7 4812
Fragments arranged by size:
4812 957 552 7
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6328
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 3686 5231 1132
Size: 97 2457 1545 2229
Fragments arranged by size:
2457 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6328
PciI A'CATG_T
Cuts at: 5227 5227
Size: 6328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6328
PfoI T'CCnGG_A
Cuts at: 2088 2088
Size: 6328
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4397 4429 5910 5942 4397
Size: 32 1481 32 4783
Fragments arranged by size:
4783 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2159 2159
Size: 6328
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6328
PsiI TTA'TAA
Cuts at: 2622 3898 2622
Size: 1276 5052
PstI C_TGCA'G
Cuts at: 830 3680 830
Size: 2850 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
SalI G'TCGA_C
Cuts at: 1936 3670 1936
Size: 1734 4594
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 4576 6288 4576
Size: 1712 4616
SfcI C'TryA_G
Cuts at: 826 1045 2137 2356 3676 4811 5492 5683 826
Size: 219 1092 219 1320 1135 681 191 1471
Fragments arranged by size:
1471 1320 1135 1092 681 219 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 2690 1700
Size: 990 5338
SmaI CCC'GGG
Cuts at: 1872 3655 1872
Size: 1783 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4432 5333 5595 820
Size: 197 926 2489 901 262 1553
Fragments arranged by size:
2489 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3625 4119 493
Size: 3132 494 2702
Fragments arranged by size:
3132 2702 494
SpeI A'CTAG_T
Cuts at: 152 3169 152
Size: 3017 3311
SphI G_CATG'C
Cuts at: 1132 3686 1132
Size: 2554 3774
SspI AAT'ATT
Cuts at: 5 52 4252 5
Size: 47 4200 2081
Fragments arranged by size:
4200 2081 47
StuI AGG'CCT
Cuts at: 1517 2645 2729 2813 3317 3569 1517
Size: 1128 84 84 504 252 4276
Fragments arranged by size:
4276 1128 504 252 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2022 2029 3767 513
Size: 552 957 7 1738 3074
Fragments arranged by size:
3074 1738 957 552 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4355 4372 4525 4710 5155 325
Size: 1255 2775 17 153 185 445 1498
Fragments arranged by size:
2775 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2821 2909 3007
Size: 274 80 33 51 1443 844 88 98
Cuts at: 3007 4574 6286 96
Size: 1567 1712 138
Fragments arranged by size:
1712 1567 1443 844 274 138 98 88
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 2517 2549 6288 6320 2111
Size: 32 374 32 3739 32 2119
Fragments arranged by size:
3739 2119 374 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6328
XbaI T'CTAG_A
Cuts at: 3664 3664
Size: 6328
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6328
XmaI C'CCGG_G
Cuts at: 1870 3653 1870
Size: 1783 4545
XmnI GAAnn'nnTTC
Cuts at: 2981 4457 2981
Size: 1476 4852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4136 276
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI