Restriction map for
pFN21ASDA1552
(Circular) (Six-base) MAPSORT of: pFN21ASDA1552.seq Check: 5379 from: 1 to: 6874
pFN21ASDA1552 6874 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2106 4146 4684 278
Size: 53 83 186 936 570 2040 538 2468
Fragments arranged by size:
2468 2040 936 570 538 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4217 1937
Size: 2280 4594
Acc65I G'GTAC_C
Cuts at: 4195 4195
Size: 6874
AclI AA'CG_TT
Cuts at: 1563 5001 5374 1563
Size: 3438 373 3063
Fragments arranged by size:
3438 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3305 3569 4937 6321 6589 1632
Size: 1673 264 1368 1384 268 1917
Fragments arranged by size:
1917 1673 1384 1368 268 264
AfeI AGC'GCT
Cuts at: 2798 2798
Size: 6874
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6677
AflIII A'CryG_T
Cuts at: 1203 4100 5773 6750 1203
Size: 2897 1673 977 1327
Fragments arranged by size:
2897 1673 1327 977
AgeI A'CCGG_T
Cuts at: 5705 5705
Size: 6874
AhdI GACnn_n'nnGTC
Cuts at: 5603 5603
Size: 6874
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6874
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6874
AlwNI CAG_nnn'CTG
Cuts at: 2269 2359 3214 3714 3987 4617 6189 2269
Size: 90 855 500 273 630 1572 2954
Fragments arranged by size:
2954 1572 855 630 500 273 90
ApaI G_GGCC'C
Cuts at: 2642 2681 2642
Size: 39 6835
ApaLI G'TGCA_C
Cuts at: 2207 3851 4931 6087 2207
Size: 1644 1080 1156 2994
Fragments arranged by size:
2994 1644 1156 1080
ApoI r'AATT_y
Cuts at: 1493 4183 4409 4561 6670 1493
Size: 2690 226 152 2109 1697
Fragments arranged by size:
2690 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5428 160
Size: 872 4396 1606
Fragments arranged by size:
4396 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2502 2702 2760 2831 3227 3265
Size: 73 90 469 200 58 71 396 38
Cuts at: 3265 3539 4199 1870
Size: 274 660 4545
Fragments arranged by size:
4545 660 469 396 274 200 90 73
71 58 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2861 2894 2861
Size: 33 6841
BamHI G'GATC_C
Cuts at: 2189 4204 4571 2189
Size: 2015 367 4492
Fragments arranged by size:
4492 2015 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2571 2755 4195 5650 618
Size: 325 217 627 784 184 1440 1455 1842
Fragments arranged by size:
1842 1455 1440 784 627 325 217 184
BanII G_rGCy'C
Cuts at: 1391 2040 2625 2642 2664 2681 2703 2709 2767
Size: 649 585 17 22 17 22 6 58
Cuts at: 2767 3530 4193 1391
Size: 763 663 4072
Fragments arranged by size:
4072 763 663 649 585 58 22 22
17 17 6
BbeI G_GCGC'C
Cuts at: 2575 2575
Size: 6874
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3210 3293 3557 928
Size: 971 1311 83 264 4245
Fragments arranged by size:
4245 1311 971 264 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5065 5099 1903
Size: 34 3128 34 3678
Fragments arranged by size:
3678 3128 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4766 5976 4766
Size: 1210 5664
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6874
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2646 3534 5485
Size: 107 122 71 631 605 974 888 1951
Cuts at: 5485 136
Size: 1525
Fragments arranged by size:
1951 1525 974 888 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6869 6869
Size: 6874
BlpI GC'TnA_GC
Cuts at: 2472 2726 2777 4291 2472
Size: 254 51 1514 5055
Fragments arranged by size:
5055 1514 254 51
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2642 2681 2693 3006 3855 4935 6091
Size: 421 431 39 12 313 849 1080 1156
Cuts at: 6091 1790
Size: 2573
Fragments arranged by size:
2573 1156 1080 849 431 421 313 39
12
BmgBI CAC'GTC
Cuts at: 1318 2847 4101 1318
Size: 1529 1254 4091
Fragments arranged by size:
4091 1529 1254
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4159 4605 5563
Size: 416 53 83 399 24 2742 446 958
Cuts at: 5563 442
Size: 1753
Fragments arranged by size:
2742 1753 958 446 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2381 2423 2582 2664 2687 2762 3497
Size: 1007 135 42 159 82 23 75 735
Cuts at: 3497 3761 3833 4034 5534 1239
Size: 264 72 201 1500 2579
Fragments arranged by size:
2579 1500 1007 735 264 201 159 135
82 75 72 42 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4042 4999 5864 6162 4042
Size: 957 865 298 4754
Fragments arranged by size:
4754 957 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5537 882
Size: 617 4038 2219
Fragments arranged by size:
4038 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4171 4665 493
Size: 711 2967 494 2702
Fragments arranged by size:
2967 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6868 6868
Size: 6874
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2572 3290 3554
Size: 53 83 186 936 570 469 718 264
Cuts at: 3554 4143 4681 5063 5698 275
Size: 589 538 382 635 1451
Fragments arranged by size:
1451 936 718 635 589 570 538 469
382 264 186 83 53
BsaWI w'CCGG_w
Cuts at: 3724 5307 5705 5979 6126 6537 3724
Size: 1583 398 274 147 411 4061
Fragments arranged by size:
4061 1583 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2280 2310 2637 2667 3347
Size: 30 165 30 877 30 327 30 680
Cuts at: 3347 3377 3611 3641 3884 3914 4090 4120 1178
Size: 30 234 30 243 30 176 30 3932
Fragments arranged by size:
3932 877 680 327 243 234 176 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2294 2672 2711 2759 2762 2765 1186
Size: 1108 378 39 48 3 3 5295
Fragments arranged by size:
5295 1108 378 48 39 3 3
BseYI C'CCAG_C
Cuts at: 2039 2476 2591 2812 3077 3222 3350 3887 3928
Size: 437 115 221 265 145 128 537 41
Cuts at: 3928 4053 6077 6573 2039
Size: 125 2024 496 2340
Fragments arranged by size:
2340 2024 537 496 437 265 221 145
128 125 115 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2350 2930 1163
Size: 573 614 580 5107
Fragments arranged by size:
5107 614 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3615 4324 5085 6113 1540
Size: 275 186 1614 709 761 1028 2301
Fragments arranged by size:
2301 1614 1028 761 709 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 3246 3765 3855 4935 5020 6091 2211
Size: 1035 519 90 1080 85 1071 2994
Fragments arranged by size:
2994 1080 1071 1035 519 90 85
BsmI GAATG_Cn'
Cuts at: 4385 4478 4385
Size: 93 6781
BsmBI CGTCTCn'nnnn_
Cuts at: 3059 3059
Size: 6874
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2211 2625 2642 2664 2681 2693
Size: 399 250 171 414 17 22 17 12
Cuts at: 2693 2703 2709 2767 3006 3246 3530 3765 3855
Size: 10 6 58 239 240 284 235 90
Cuts at: 3855 4193 4935 5020 6091 1391
Size: 338 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 414 399 338 284 250
240 239 235 171 90 85 58 22
17 17 12 10 6
BspEI T'CCGG_A
Cuts at: 6537 6537
Size: 6874
BspHI T'CATG_A
Cuts at: 4763 6493 4763
Size: 1730 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2936 3380 3644 3831 4229 844
Size: 2092 444 264 187 398 3489
Fragments arranged by size:
3489 2092 444 398 264 187
BsrBI CCG'CTC
Cuts at: 1394 1823 4036 4761 1394
Size: 429 2213 725 3507
Fragments arranged by size:
3507 2213 725 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5369 5543 65
Size: 1183 4121 174 1396
Fragments arranged by size:
4121 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2069 2734 3333 3597 3687 5518 5705
Size: 110 259 665 599 264 90 1831 187
Cuts at: 5705 1700
Size: 2869
Fragments arranged by size:
2869 1831 665 599 264 259 187 110
90
BsrGI T'GTAC_A
Cuts at: 96 2415 96
Size: 2319 4555
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6874
BssSI C'ACGA_G
Cuts at: 2873 3854 4934 5946 2873
Size: 981 1080 1012 3801
Fragments arranged by size:
3801 1080 1012 981
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2269 3396 3660 3714 3987 794
Size: 1475 1127 264 54 273 3681
Fragments arranged by size:
3681 1475 1127 273 264 54
BstBI TT'CG_AA
Cuts at: 2007 5678 2007
Size: 3671 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6874
BstYI r'GATC_y
Cuts at: 1972 2189 2265 3796 4204 4571 4956 4973 6425
Size: 217 76 1531 408 367 385 17 1452
Cuts at: 6425 6511 6523 6869 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1531 1452 408 385 367 346 217
86 76 17 12
Bsu36I CC'TnA_GG
Cuts at: 2369 2634 2694 2840 3485 3821 2369
Size: 265 60 146 645 336 5422
Fragments arranged by size:
5422 645 336 265 146 60
BtgI C'CryG_G
Cuts at: 513 1065 2681 3060 4047 513
Size: 552 1616 379 987 3340
Fragments arranged by size:
3340 1616 987 552 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6135
Fragments arranged by size:
6135 683 56
BtsI GCAGTG_nn'
Cuts at: 2209 2439 2533 2693 2771 3004 3448 3712 3788
Size: 230 94 160 78 233 444 264 76
Cuts at: 3788 3985 4390 5184 5204 2209
Size: 197 405 794 20 3879
Fragments arranged by size:
3879 794 444 405 264 233 230 197
160 94 78 76 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6874
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6839
DraI TTT'AAA
Cuts at: 4178 4178
Size: 6874
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5881 809
Size: 507 4565 1802
Fragments arranged by size:
4565 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 3612 4321
Size: 54 1425 50 275 18 241 1541 709
Cuts at: 4321 5210 6839 8
Size: 889 1629 43
Fragments arranged by size:
1629 1541 1425 889 709 275 241 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3612 4321 1537
Size: 275 1800 709 4090
Fragments arranged by size:
4090 1800 709 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4804 1353
Size: 3451 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5461 5835 5981 387
Size: 1064 138 3872 374 146 1280
Fragments arranged by size:
3872 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2381 2423 2582 2664 2687 2762
Size: 393 614 135 42 159 82 23 75
Cuts at: 2762 3305 3497 3569 3761 3833 4034 4937 5534
Size: 543 192 72 192 72 201 903 597
Cuts at: 5534 6321 6589 1239
Size: 787 268 1524
Fragments arranged by size:
1524 903 787 614 597 543 393 268
201 192 192 159 135 82 75 72
72 42 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2606 2744 2606
Size: 138 6736
EcoO109I rG'GnC_Cy
Cuts at: 2630 2638 2639 3028 3155 3232 3272 3481 3536
Size: 8 1 389 127 77 40 209 55
Cuts at: 3536 3745 4063 2630
Size: 209 318 5441
Fragments arranged by size:
5441 389 318 209 209 127 77 55
40 8 1
EcoRI G'AATT_C
Cuts at: 4183 4561 4183
Size: 378 6496
FseI GG_CCGG'CC
Cuts at: 2073 2073
Size: 6874
FspI TGC'GCA
Cuts at: 3707 3980 5380 3707
Size: 273 1400 5201
Fragments arranged by size:
5201 1400 273
HaeII r_GCGC'y
Cuts at: 1678 2575 2800 6021 1678
Size: 897 225 3221 2531
Fragments arranged by size:
3221 2531 897 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2280 2312 3183 3215 4090
Size: 32 135 32 708 32 871 32 875
Cuts at: 4090 4122 4145 4177 5515 5547 5589 5621 1373
Size: 32 23 32 1338 32 42 32 2626
Fragments arranged by size:
2626 1338 875 871 708 135 42 32
32 32 32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 4218 4464 669
Size: 988 281 2280 246 3079
Fragments arranged by size:
3079 2280 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6874
HpaI GTT'AAC
Cuts at: 4464 4464
Size: 6874
KasI G'GCGC_C
Cuts at: 2571 2571
Size: 6874
KpnI G_GTAC'C
Cuts at: 4199 4199
Size: 6874
MfeI C'AATT_G
Cuts at: 4473 4473
Size: 6874
MluI A'CGCG_T
Cuts at: 6750 6750
Size: 6874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2190 5988 6172 1305
Size: 885 3798 184 2007
Fragments arranged by size:
3798 2007 885 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5052
Fragments arranged by size:
5052 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2522 3701 3974 4832 5191 5350 518
Size: 648 1356 1179 273 858 359 159 2042
Fragments arranged by size:
2042 1356 1179 858 648 359 273 159
MspA1I CmG'CkG
Cuts at: 1606 2983 3493 3520 3757 4087 4290 4967 6115
Size: 1377 510 27 237 330 203 677 1148
Cuts at: 6115 6360 1606
Size: 245 2120
Fragments arranged by size:
2120 1377 1148 677 510 330 245 237
203 27
NaeI GCC'GGC
Cuts at: 2071 2736 3689 2071
Size: 665 953 5256
Fragments arranged by size:
5256 953 665
NarI GG'CG_CC
Cuts at: 2572 2572
Size: 6874
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6322
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6874
NgoMIV G'CCGG_C
Cuts at: 2069 2734 3687 2069
Size: 665 953 5256
Fragments arranged by size:
5256 953 665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6874
NotI GC'GGCC_GC
Cuts at: 4321 4321
Size: 6874
NspI r_CATG'y
Cuts at: 1132 1229 2471 4232 5777 1132
Size: 97 1242 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 1242 97
PasI CC'CwG_GG
Cuts at: 1348 2130 2626 2974 3092 3295 3418 3559 3682
Size: 782 496 348 118 203 123 141 123
Cuts at: 3682 3832 3955 1348
Size: 150 123 4267
Fragments arranged by size:
4267 782 496 348 203 150 141 123
123 123 118
PciI A'CATG_T
Cuts at: 5773 5773
Size: 6874
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3253 1108
Size: 2145 4729
PmeI GTTT'AAAC
Cuts at: 4178 4178
Size: 6874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4943 4975 6456 6488 4943
Size: 32 1481 32 5329
Fragments arranged by size:
5329 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2630 3155 4063 2630
Size: 525 908 5441
Fragments arranged by size:
5441 908 525
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6874
PsiI TTA'TAA
Cuts at: 4444 4444
Size: 6874
PspOMI G'GGCC_C
Cuts at: 2638 2677 2638
Size: 39 6835
PspXI vC'TCGA_Gb
Cuts at: 2831 2831
Size: 6874
PstI C_TGCA'G
Cuts at: 830 3075 4226 830
Size: 2245 1151 3478
Fragments arranged by size:
3478 2245 1151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6874
PvuII CAG'CTG
Cuts at: 4087 4087
Size: 6874
SalI G'TCGA_C
Cuts at: 1936 4216 1936
Size: 2280 4594
SanDI GG'GwC_CC
Cuts at: 3155 3155
Size: 6874
SbfI CC_TGCA'GG
Cuts at: 4226 4226
Size: 6874
ScaI AGT'ACT
Cuts at: 5122 6834 5122
Size: 1712 5162
SfcI C'TryA_G
Cuts at: 826 1045 3071 3868 4222 5357 6038 6229 826
Size: 219 2026 797 354 1135 681 191 1471
Fragments arranged by size:
2026 1471 1135 797 681 354 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2646 2646
Size: 6874
SfoI GGC'GCC
Cuts at: 2573 2573
Size: 6874
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6874
SmaI CCC'GGG
Cuts at: 1872 3267 4201 1872
Size: 1395 934 4545
Fragments arranged by size:
4545 1395 934
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2831 4021 4978 5879 6141 820
Size: 197 926 888 1190 957 901 262 1553
Fragments arranged by size:
1553 1190 957 926 901 888 262 197
SnaBI TAC'GTA
Cuts at: 493 4171 4665 493
Size: 3678 494 2702
Fragments arranged by size:
3678 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6874
SphI G_CATG'C
Cuts at: 1132 4232 1132
Size: 3100 3774
SspI AAT'ATT
Cuts at: 5 52 4798 5
Size: 47 4746 2081
Fragments arranged by size:
4746 2081 47
StuI AGG'CCT
Cuts at: 1517 2508 2559 3175 3474 4011 4019 1517
Size: 991 51 616 299 537 8 4372
Fragments arranged by size:
4372 991 616 537 299 51 8
StyI C'CwwG_G
Cuts at: 513 1065 4313 513
Size: 552 3248 3074
Fragments arranged by size:
3248 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2987 3431 3695 3735 4901 4918 5071
Size: 1255 1407 444 264 40 1166 17 153
Cuts at: 5071 5256 5701 325
Size: 185 445 1498
Fragments arranged by size:
1498 1407 1255 1166 445 444 264 185
153 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2415 2448 3900
Size: 274 80 33 51 1443 438 33 1452
Cuts at: 3900 5120 6832 96
Size: 1220 1712 138
Fragments arranged by size:
1712 1452 1443 1220 438 274 138 80
51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2637 2669 2970 3002 6834 6866 2637
Size: 32 301 32 3832 32 2645
Fragments arranged by size:
3832 2645 301 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6874
XbaI T'CTAG_A
Cuts at: 4210 4210
Size: 6874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2177 2716 4124 2177
Size: 539 1408 4927
Fragments arranged by size:
4927 1408 539
XhoI C'TCGA_G
Cuts at: 1943 2831 1943
Size: 888 5986
XmaI C'CCGG_G
Cuts at: 1870 3265 4199 1870
Size: 1395 934 4545
Fragments arranged by size:
4545 1395 934
XmnI GAAnn'nnTTC
Cuts at: 4074 5003 4074
Size: 929 5945
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2104 4144 4682 276
Size: 53 83 186 936 570 2040 538 2468
Fragments arranged by size:
2468 2040 936 570 538 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FspAI
NruI NsiI PacI PfoI PmlI PsrI RsrII SacI
SacII SapI SexAI SrfI SwaI