Restriction map for
pFN21ASDA1547
(Circular) (Six-base) MAPSORT of: pFN21ASDA1547.seq Check: 8863 from: 1 to: 7009
pFN21ASDA1547 7009 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2320 4086 2320
Size: 1766 5243
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3707 4819 278
Size: 53 83 186 936 2171 1112 2468
Fragments arranged by size:
2468 2171 1112 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3489 4352 1937
Size: 1552 863 4594
Fragments arranged by size:
4594 1552 863
Acc65I G'GTAC_C
Cuts at: 4330 4330
Size: 7009
AclI AA'CG_TT
Cuts at: 1563 5136 5509 1563
Size: 3573 373 3063
Fragments arranged by size:
3573 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5072 6456 6724 1632
Size: 3440 1384 268 1917
Fragments arranged by size:
3440 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6812
AflIII A'CryG_T
Cuts at: 1203 5908 6885 1203
Size: 4705 977 1327
Fragments arranged by size:
4705 1327 977
AgeI A'CCGG_T
Cuts at: 5840 5840
Size: 7009
AhdI GACnn_n'nnGTC
Cuts at: 5738 5738
Size: 7009
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7009
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7009
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2011 2043 2011
Size: 32 6977
AlwNI CAG_nnn'CTG
Cuts at: 2128 3209 3814 4265 4752 6324 2128
Size: 1081 605 451 487 1572 2813
Fragments arranged by size:
2813 1572 1081 605 487 451
ApaLI G'TGCA_C
Cuts at: 5066 6222 5066
Size: 1156 5853
ApoI r'AATT_y
Cuts at: 1493 2078 2117 4318 4544 4696 6805 1493
Size: 585 39 2201 226 152 2109 1697
Fragments arranged by size:
2201 2109 1697 585 226 152 39
AseI AT'TA_AT
Cuts at: 160 1032 5563 160
Size: 872 4531 1606
Fragments arranged by size:
4531 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7009
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2802 3406 4334 1870
Size: 73 117 742 604 928 4545
Fragments arranged by size:
4545 928 742 604 117 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2725 2758 2725
Size: 33 6976
BamHI G'GATC_C
Cuts at: 4339 4706 4339
Size: 367 6642
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2531 3011 3091 3146 3875
Size: 325 217 627 744 480 80 55 729
Cuts at: 3875 4330 5785 618
Size: 455 1455 1842
Fragments arranged by size:
1842 1455 744 729 627 480 455 325
217 80 55
BanII G_rGCy'C
Cuts at: 1391 2672 2967 3033 3551 4328 1391
Size: 1281 295 66 518 777 4072
Fragments arranged by size:
4072 1281 777 518 295 66
BbeI G_GCGC'C
Cuts at: 2535 3095 3150 2535
Size: 560 55 6394
Fragments arranged by size:
6394 560 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2189 928
Size: 971 290 5748
Fragments arranged by size:
5748 971 290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3610 3644 4111 4145 5200 5234 1903
Size: 34 1673 34 467 34 1055 34 3678
Fragments arranged by size:
3678 1673 1055 467 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2037 4901 6111 2037
Size: 2864 1210 2935
Fragments arranged by size:
2935 2864 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7009
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2891 3537 5620
Size: 107 122 71 631 605 1219 646 2083
Cuts at: 5620 136
Size: 1525
Fragments arranged by size:
2083 1525 1219 646 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3516 3969 7004 3516
Size: 453 3035 3521
Fragments arranged by size:
3521 3035 453
BlpI GC'TnA_GC
Cuts at: 4426 4426
Size: 7009
Bme1580I G_kGCm'C
Cuts at: 1790 3372 5070 6226 1790
Size: 1582 1698 1156 2573
Fragments arranged by size:
2573 1698 1582 1156
BmgBI CAC'GTC
Cuts at: 1318 3487 1318
Size: 2169 4840
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2489 2537 4005
Size: 416 53 83 399 24 1072 48 1468
Cuts at: 4005 4149 4740 5698 442
Size: 144 591 958 1753
Fragments arranged by size:
1753 1468 1072 958 591 416 399 144
83 53 48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 2459 2466 2991 3333 4186 5669 1239
Size: 1151 69 7 525 342 853 1483 2579
Fragments arranged by size:
2579 1483 1151 853 525 342 69 7
Bpu10I CC'TnA_GC
Cuts at: 3032 3032
Size: 7009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2746 2951 3277 4088 4240 5134 5999 6297 2746
Size: 205 326 811 152 894 865 298 3458
Fragments arranged by size:
3458 894 865 811 326 298 205 152
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2477 5672 882
Size: 617 978 3195 2219
Fragments arranged by size:
3195 2219 978 617
BsaAI yAC'GTr
Cuts at: 493 1204 4306 4800 493
Size: 711 3102 494 2702
Fragments arranged by size:
3102 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7003 7003
Size: 7009
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2532 2997 3092 3147
Size: 53 83 186 936 999 465 95 55
Cuts at: 3147 3384 3704 3747 4172 4816 5198 5833 275
Size: 237 320 43 425 644 382 635 1451
Fragments arranged by size:
1451 999 936 644 635 465 425 382
320 237 186 95 83 55 53 43
BsaWI w'CCGG_w
Cuts at: 5442 5840 6114 6261 6672 5442
Size: 398 274 147 411 5779
Fragments arranged by size:
5779 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2936 2966 3437 3467 3578
Size: 30 165 30 1533 30 471 30 111
Cuts at: 3578 3608 1178
Size: 30 4579
Fragments arranged by size:
4579 1533 471 165 111 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2726 1186
Size: 1540 5469
BseYI C'CCAG_C
Cuts at: 3558 3979 6212 6708 3558
Size: 421 2233 496 3859
Fragments arranged by size:
3859 2233 496 421
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3785 4028 4058 4064 1163
Size: 573 2049 243 30 6 4108
Fragments arranged by size:
4108 2049 573 243 30 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3224 4459 5220 6248 1540
Size: 275 186 1223 1235 761 1028 2301
Fragments arranged by size:
2301 1235 1223 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2322 4098 4139 5070 5155 6226 2322
Size: 1776 41 931 85 1071 3105
Fragments arranged by size:
3105 1776 1071 931 85 41
BsmI GAATG_Cn'
Cuts at: 4520 4613 4520
Size: 93 6916
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2672 2967 3033 3372 3551 4098
Size: 399 532 350 295 66 339 179 547
Cuts at: 4098 4139 4328 5070 5155 6226 1391
Size: 41 189 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 547 532 399 350 339
295 189 179 85 66 41
BspEI T'CCGG_A
Cuts at: 6672 6672
Size: 7009
BspHI T'CATG_A
Cuts at: 4898 6628 4898
Size: 1730 5279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2320 2350 3807 3933 4086 4364 844
Size: 1476 30 1457 126 153 278 3489
Fragments arranged by size:
3489 1476 1457 278 153 126 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 4896 1394
Size: 429 1771 1302 3507
Fragments arranged by size:
3507 1771 1302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3783 5504 5678 65
Size: 1183 2535 1721 174 1396
Fragments arranged by size:
2535 1721 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3043 3155 5653 5840 1700
Size: 110 1233 112 2498 187 2869
Fragments arranged by size:
2869 2498 1233 187 112 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7009
BssHII G'CGCG_C
Cuts at: 1925 2350 1925
Size: 425 6584
BssSI C'ACGA_G
Cuts at: 3478 4082 5069 6081 3478
Size: 604 987 1012 4406
Fragments arranged by size:
4406 1012 987 604
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3209 3367 3507 794
Size: 2415 158 140 4296
Fragments arranged by size:
4296 2415 158 140
BstBI TT'CG_AA
Cuts at: 2007 5813 2007
Size: 3806 3203
BstEII G'GTnAC_C
Cuts at: 1184 2362 1184
Size: 1178 5831
BstXI CCAn_nnnn'nTGG
Cuts at: 3842 3842
Size: 7009
BstYI r'GATC_y
Cuts at: 1972 3516 3969 4339 4706 5091 5108 6560 6646
Size: 1544 453 370 367 385 17 1452 86
Cuts at: 6646 6658 7004 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1544 1452 453 385 370 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3439 513
Size: 552 2374 4083
Fragments arranged by size:
4083 2374 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3789 487
Size: 683 56 2563 3707
Fragments arranged by size:
3707 2563 683 56
BtsI GCAGTG_nn'
Cuts at: 2349 4525 5319 5339 2349
Size: 2176 794 20 4019
Fragments arranged by size:
4019 2176 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7009
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6974
DraI TTT'AAA
Cuts at: 2077 4313 2077
Size: 2236 4773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3752 6016 809
Size: 507 2436 2264 1802
Fragments arranged by size:
2436 2264 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3217 3732 4456
Size: 54 1425 50 275 18 1387 515 724
Cuts at: 4456 5345 6974 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1387 889 724 515 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4456 1537
Size: 275 2644 4090
Fragments arranged by size:
4090 2644 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 2902 4939 1353
Size: 911 638 2037 3423
Fragments arranged by size:
3423 2037 911 638
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2723 5596 5970 6116 387
Size: 1064 138 1134 2873 374 146 1280
Fragments arranged by size:
2873 1280 1134 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 2459 2466 2991 3333 4186 5072
Size: 393 758 69 7 525 342 853 886
Cuts at: 5072 5669 6456 6724 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 886 853 787 758 597 525 393
342 268 69 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3929 3929
Size: 7009
EcoO109I rG'GnC_Cy
Cuts at: 2788 3395 3664 3796 3895 3922 2788
Size: 607 269 132 99 27 5875
Fragments arranged by size:
5875 607 269 132 99 27
EcoRI G'AATT_C
Cuts at: 2117 4318 4696 2117
Size: 2201 378 4430
Fragments arranged by size:
4430 2201 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 4169 2740
Size: 1429 5580
FspI TGC'GCA
Cuts at: 3059 5515 3059
Size: 2456 4553
HaeII r_GCGC'y
Cuts at: 1678 2200 2535 3095 3150 3189 6156 1678
Size: 522 335 560 55 39 2967 2531
Fragments arranged by size:
2967 2531 560 522 335 55 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2591 2623 2746 2778 2936
Size: 32 135 32 1019 32 123 32 158
Cuts at: 2936 2968 3656 3688 3689 3721 5650 5682 5724
Size: 32 688 32 1 32 1929 32 42
Cuts at: 5724 5756 1373
Size: 32 2626
Fragments arranged by size:
2626 1929 1019 688 158 135 123 42
32 32 32 32 32 32 32 32
32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4353 4599 669
Size: 988 281 2415 246 3079
Fragments arranged by size:
3079 2415 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7009
HpaI GTT'AAC
Cuts at: 4599 4599
Size: 7009
KasI G'GCGC_C
Cuts at: 2531 3091 3146 2531
Size: 560 55 6394
Fragments arranged by size:
6394 560 55
KpnI G_GTAC'C
Cuts at: 4334 4334
Size: 7009
MfeI C'AATT_G
Cuts at: 4608 4608
Size: 7009
MluI A'CGCG_T
Cuts at: 6885 6885
Size: 7009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2997 6123 6307 1305
Size: 1692 3126 184 2007
Fragments arranged by size:
3126 2007 1692 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3734 10
Size: 54 1425 343 1902 3285
Fragments arranged by size:
3285 1902 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 3342 3840 4967 5326 5485 518
Size: 648 1077 1099 498 1127 359 159 2042
Fragments arranged by size:
2042 1127 1099 1077 648 498 359 159
MspA1I CmG'CkG
Cuts at: 1606 3197 3209 3562 3852 4072 4238 4425 5102
Size: 1591 12 353 290 220 166 187 677
Cuts at: 5102 6250 6495 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1591 1148 677 353 290 245 220
187 166 12
NaeI GCC'GGC
Cuts at: 3045 3157 3045
Size: 112 6897
NarI GG'CG_CC
Cuts at: 2532 3092 3147 2532
Size: 560 55 6394
Fragments arranged by size:
6394 560 55
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6457
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7009
NgoMIV G'CCGG_C
Cuts at: 3043 3155 3043
Size: 112 6897
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7009
NotI GC'GGCC_GC
Cuts at: 4456 4456
Size: 7009
NspI r_CATG'y
Cuts at: 1132 1229 4367 5912 1132
Size: 97 3138 1545 2229
Fragments arranged by size:
3138 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2536 2960 3026 1348
Size: 1188 424 66 5331
Fragments arranged by size:
5331 1188 424 66
PciI A'CATG_T
Cuts at: 5908 5908
Size: 7009
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3814 3885 1108
Size: 2706 71 4232
Fragments arranged by size:
4232 2706 71
PfoI T'CCnGG_A
Cuts at: 2482 3310 2482
Size: 828 6181
PmeI GTTT'AAAC
Cuts at: 4313 4313
Size: 7009
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5078 5110 6591 6623 5078
Size: 32 1481 32 5464
Fragments arranged by size:
5464 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2788 3664 3796 3922 2788
Size: 876 132 126 5875
Fragments arranged by size:
5875 876 132 126
PshAI GACnn'nnGTC
Cuts at: 1613 2934 3815 1613
Size: 1321 881 4807
Fragments arranged by size:
4807 1321 881
PsiI TTA'TAA
Cuts at: 4579 4579
Size: 7009
PstI C_TGCA'G
Cuts at: 830 3205 3773 4361 830
Size: 2375 568 588 3478
Fragments arranged by size:
3478 2375 588 568
PvuI CG_AT'CG
Cuts at: 2001 3224 2001
Size: 1223 5786
PvuII CAG'CTG
Cuts at: 3562 3852 4072 3562
Size: 290 220 6499
Fragments arranged by size:
6499 290 220
SalI G'TCGA_C
Cuts at: 1936 4351 1936
Size: 2415 4594
SbfI CC_TGCA'GG
Cuts at: 4361 4361
Size: 7009
ScaI AGT'ACT
Cuts at: 5257 6969 5257
Size: 1712 5297
SfcI C'TryA_G
Cuts at: 826 1045 2680 3201 3769 3944 4357 5492 6173
Size: 219 1635 521 568 175 413 1135 681
Cuts at: 6173 6364 826
Size: 191 1471
Fragments arranged by size:
1635 1471 1135 681 568 521 413 219
191 175
SfoI GGC'GCC
Cuts at: 2533 3093 3148 2533
Size: 560 55 6394
Fragments arranged by size:
6394 560 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7009
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7009
SmaI CCC'GGG
Cuts at: 1872 2062 2804 4336 1872
Size: 190 742 1532 4545
Fragments arranged by size:
4545 1532 742 190
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2725 2966 3292 4103 4219 5113
Size: 197 926 782 241 326 811 116 894
Cuts at: 5113 6014 6276 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 894 811 782 326 262
241 197 116
SnaBI TAC'GTA
Cuts at: 493 4306 4800 493
Size: 3813 494 2702
Fragments arranged by size:
3813 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7009
SphI G_CATG'C
Cuts at: 1132 4367 1132
Size: 3235 3774
SspI AAT'ATT
Cuts at: 5 52 4933 5
Size: 47 4881 2081
Fragments arranged by size:
4881 2081 47
StuI AGG'CCT
Cuts at: 1517 3285 1517
Size: 1768 5241
StyI C'CwwG_G
Cuts at: 513 1065 2932 2987 4448 513
Size: 552 1867 55 1461 3074
Fragments arranged by size:
3074 1867 1461 552 55
SwaI ATTT'AAAT
Cuts at: 2077 2077
Size: 7009
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5036 5053 5206 5391 5836 325
Size: 1255 3456 17 153 185 445 1498
Fragments arranged by size:
3456 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 5255 6967
Size: 274 80 33 51 1443 234 3044 1712
Cuts at: 6967 96
Size: 138
Fragments arranged by size:
3044 1712 1443 274 234 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3437 3469 6969 7001 3437
Size: 32 3500 32 3445
Fragments arranged by size:
3500 3445 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7009
XbaI T'CTAG_A
Cuts at: 4345 4345
Size: 7009
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3559 3664 3559
Size: 105 6904
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7009
XmaI C'CCGG_G
Cuts at: 1870 2060 2802 4334 1870
Size: 190 742 1532 4545
Fragments arranged by size:
4545 1532 742 190
XmnI GAAnn'nnTTC
Cuts at: 5138 5138
Size: 7009
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3705 4817 276
Size: 53 83 186 936 2171 1112 2468
Fragments arranged by size:
2468 2171 1112 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI