Restriction map for
pFN21ASDA1542
(Circular) (Six-base) MAPSORT of: pFN21ASDA1542.seq Check: 3542 from: 1 to: 9676
pFN21ASDA1542 9676 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2096 2096
Size: 9676
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5288 7486 278
Size: 53 83 186 936 3752 2198 2468
Fragments arranged by size:
3752 2468 2198 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4337 4509 7019 1937
Size: 2400 172 2510 4594
Fragments arranged by size:
4594 2510 2400 172
Acc65I G'GTAC_C
Cuts at: 2643 6997 2643
Size: 4354 5322
AclI AA'CG_TT
Cuts at: 1563 7803 8176 1563
Size: 6240 373 3063
Fragments arranged by size:
6240 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2271 3877 4043 5957 6515 7739 9123 9391
Size: 639 1606 166 1914 558 1224 1384 268
Cuts at: 9391 1632
Size: 1917
Fragments arranged by size:
1917 1914 1606 1384 1224 639 558 268
166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9479
AflIII A'CryG_T
Cuts at: 1203 4416 5701 5827 8575 9552 1203
Size: 3213 1285 126 2748 977 1327
Fragments arranged by size:
3213 2748 1327 1285 977 126
AgeI A'CCGG_T
Cuts at: 8507 8507
Size: 9676
AhdI GACnn_n'nnGTC
Cuts at: 8405 8405
Size: 9676
AleI CACnn'nnGTG
Cuts at: 1166 2990 1166
Size: 1824 7852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2359 4022 4247 6744 6836 1184
Size: 1175 1663 225 2497 92 4024
Fragments arranged by size:
4024 2497 1663 1175 225 92
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2681 2713 2879 2911 6398 6399 6430 6431 2681
Size: 32 166 32 3487 1 31 1 5926
Fragments arranged by size:
5926 3487 166 32 32 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 3240 3530 3845 3912 4149 4751 4804 6574 7419
Size: 290 315 67 237 602 53 1770 845
Cuts at: 7419 8991 3240
Size: 1572 3925
Fragments arranged by size:
3925 1770 1572 845 602 315 290 237
67 53
ApaI G_GGCC'C
Cuts at: 5264 5264
Size: 9676
ApaLI G'TGCA_C
Cuts at: 4250 7733 8889 4250
Size: 3483 1156 5037
Fragments arranged by size:
5037 3483 1156
ApoI r'AATT_y
Cuts at: 1493 6985 7211 7363 9472 1493
Size: 5492 226 152 2109 1697
Fragments arranged by size:
5492 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8230 160
Size: 872 7198 1606
Fragments arranged by size:
7198 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9676
AvaI C'yCGr_G
Cuts at: 1870 1943 2817 2832 2933 3705 4236 4244 4562
Size: 73 874 15 101 772 531 8 318
Cuts at: 4562 5110 5376 5487 6285 6315 6410 7001 1870
Size: 548 266 111 798 30 95 591 4545
Fragments arranged by size:
4545 874 798 772 591 548 531 318
266 111 101 95 73 30 15 8
BamHI G'GATC_C
Cuts at: 3343 7006 7373 3343
Size: 3663 367 5646
Fragments arranged by size:
5646 3663 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2643 2680 2791 2928 3480
Size: 325 217 627 856 37 111 137 552
Cuts at: 3480 3496 3814 3923 4006 4178 4231 6041 6091
Size: 16 318 109 83 172 53 1810 50
Cuts at: 6091 6112 6289 6997 8452 618
Size: 21 177 708 1455 1842
Fragments arranged by size:
1842 1810 1455 856 708 627 552 325
318 217 177 172 137 111 109 83
53 50 37 21 16
BanII G_rGCy'C
Cuts at: 1391 2063 2500 3113 3273 3412 3728 3771 4245
Size: 672 437 613 160 139 316 43 474
Cuts at: 4245 4698 4788 4842 4983 5111 5264 5309 5885
Size: 453 90 54 141 128 153 45 576
Cuts at: 5885 6217 6283 6995 1391
Size: 332 66 712 4072
Fragments arranged by size:
4072 712 672 613 576 474 453 437
332 316 160 153 141 139 128 90
66 54 45 43
BbeI G_GCGC'C
Cuts at: 2932 3484 3818 6116 2932
Size: 552 334 2298 6492
Fragments arranged by size:
6492 2298 552 334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2199 2223 2232 2259 2931 3047 3055
Size: 971 300 24 9 27 672 116 8
Cuts at: 3055 4490 5670 6015 6135 6659 928
Size: 1435 1180 345 120 524 3945
Fragments arranged by size:
3945 1435 1180 971 672 524 345 300
120 116 27 24 9 8
BbvCI CC'TCA_GC
Cuts at: 3614 6087 3614
Size: 2473 7203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4477 4511 5688 5722 7867 7901 1903
Size: 34 2540 34 1177 34 2145 34 3678
Fragments arranged by size:
3678 2540 2145 1177 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4059 7568 8778 4059
Size: 3509 1210 4957
Fragments arranged by size:
4957 3509 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9676
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2963 3626 3823
Size: 107 122 71 631 605 1291 663 197
Cuts at: 3823 3848 4760 5268 5732 5963 6287 8287 136
Size: 25 912 508 464 231 324 2000 1525
Fragments arranged by size:
2000 1525 1291 912 663 631 605 508
464 324 231 197 122 107 71 25
BglII A'GATC_T
Cuts at: 2231 3122 3966 6828 9671 2231
Size: 891 844 2862 2843 2236
Fragments arranged by size:
2862 2843 2236 891 844
BlpI GC'TnA_GC
Cuts at: 3112 3770 7093 3112
Size: 658 3323 5695
Fragments arranged by size:
5695 3323 658
Bme1580I G_kGCm'C
Cuts at: 1790 2404 2796 4181 4236 4254 5264 5539 6044
Size: 614 392 1385 55 18 1010 275 505
Cuts at: 6044 6292 6588 7737 8893 1790
Size: 248 296 1149 1156 2573
Fragments arranged by size:
2573 1385 1156 1149 1010 614 505 392
296 275 248 55 18
BmgBI CAC'GTC
Cuts at: 1318 4948 5830 1318
Size: 3630 882 5164
Fragments arranged by size:
5164 3630 882
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2525 4137 4316
Size: 416 53 83 399 24 1108 1612 179
Cuts at: 4316 6090 6384 6575 7407 8365 442
Size: 1774 294 191 832 958 1753
Fragments arranged by size:
1774 1753 1612 1108 958 832 416 399
294 191 179 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 6674 1056
Size: 5618 4058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2785 3426 3648 4477 4780 2785
Size: 641 222 829 303 7681
Fragments arranged by size:
7681 829 641 303 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2657 3618 4381 4525 5170 5182 5429
Size: 1055 363 961 763 144 645 12 247
Cuts at: 5429 5780 8336 1239
Size: 351 2556 2579
Fragments arranged by size:
2579 2556 1055 961 763 645 363 351
247 144 12
Bpu10I CC'TnA_GC
Cuts at: 2981 3614 4982 6062 6087 2981
Size: 633 1368 1080 25 6570
Fragments arranged by size:
6570 1368 1080 633 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 5830 6463 7801 8666 8964 3955
Size: 1875 633 1338 865 298 4667
Fragments arranged by size:
4667 1875 1338 865 633 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2364 2773 5484 8339 882
Size: 617 865 409 2711 2855 2219
Fragments arranged by size:
2855 2711 2219 865 617 409
BsaAI yAC'GTr
Cuts at: 493 1204 6397 6892 6973 7467 493
Size: 711 5193 495 81 494 2702
Fragments arranged by size:
5193 2702 711 495 494 81
BsaBI GATnn'nnATC
Cuts at: 9670 9670
Size: 9676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2204 2387 2929 3011
Size: 53 83 186 936 671 183 542 82
Cuts at: 3011 3481 3815 4862 5285 5853 6113 7483 7865
Size: 470 334 1047 423 568 260 1370 382
Cuts at: 7865 8500 275
Size: 635 1451
Fragments arranged by size:
1451 1370 1047 936 671 635 568 542
470 423 382 334 260 186 183 83
82 53
BsaWI w'CCGG_w
Cuts at: 2883 2889 3086 4353 5493 6403 8109 8507 8781
Size: 6 197 1267 1140 910 1706 398 274
Cuts at: 8781 8928 9339 2883
Size: 147 411 3220
Fragments arranged by size:
3220 1706 1267 1140 910 411 398 274
197 147 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2587 2617 2879 2909 1178
Size: 30 165 30 1184 30 262 30 7945
Fragments arranged by size:
7945 1184 262 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 2570 2573 2576 2774 2777 3530 3533
Size: 1039 345 3 3 198 3 753 3
Cuts at: 3533 4796 4865 4904 5153 6191 6662 6710 6752
Size: 1263 69 39 249 1038 471 48 42
Cuts at: 6752 6806 6956 1186
Size: 54 150 3906
Fragments arranged by size:
3906 1263 1039 1038 753 471 345 249
198 150 69 54 48 42 39 3
3 3 3
BseYI C'CCAG_C
Cuts at: 3211 3538 3574 4273 4280 4937 5629 5953 6471
Size: 327 36 699 7 657 692 324 518
Cuts at: 6471 6601 8879 9375 3211
Size: 130 2278 496 3512
Fragments arranged by size:
3512 2278 699 692 657 518 496 327
324 130 36 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4661 4764 6698 6842 1163
Size: 573 2925 103 1934 144 3997
Fragments arranged by size:
3997 2925 1934 573 144 103
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2602 5294 5300 5727 7126 7887
Size: 275 186 601 2692 6 427 1399 761
Cuts at: 7887 8915 1540
Size: 1028 2301
Fragments arranged by size:
2692 2301 1399 1028 761 601 427 275
186 6
BsiHKAI G_wGCw'C
Cuts at: 2500 2987 3113 3712 3764 4254 4983 5064 5111
Size: 487 126 599 52 490 729 81 47
Cuts at: 5111 5253 5331 5393 5532 5547 5885 7737 7822
Size: 142 78 62 139 15 338 1852 85
Cuts at: 7822 8893 2500
Size: 1071 3283
Fragments arranged by size:
3283 1852 1071 729 599 490 487 338
142 139 126 85 81 78 62 52
47 15
BsiWI C'GTAC_G
Cuts at: 6888 6888
Size: 9676
BsmI GAATG_Cn'
Cuts at: 2363 2449 2660 7187 7280 2363
Size: 86 211 4527 93 4759
Fragments arranged by size:
4759 4527 211 93 86
BsmBI CGTCTCn'nnnn_
Cuts at: 3397 3463 3397
Size: 66 9610
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2404 2500 2796 2987 3113 3273
Size: 399 273 341 96 296 191 126 160
Cuts at: 3273 3412 3712 3728 3764 3771 4181 4236 4245
Size: 139 300 16 36 7 410 55 9
Cuts at: 4245 4254 4698 4788 4842 4983 5064 5111 5253
Size: 9 444 90 54 141 81 47 142
Cuts at: 5253 5264 5309 5331 5393 5532 5539 5547 5885
Size: 11 45 22 62 139 7 8 338
Cuts at: 5885 6044 6217 6283 6292 6588 6995 7737 7822
Size: 159 173 66 9 296 407 742 85
Cuts at: 7822 8893 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 444 410 407 399 341
338 300 296 296 273 191 173 160
159 142 141 139 139 126 96 90
85 81 66 62 55 54 47 45
36 22 16 11 9 9 9 8
7 7
BspEI T'CCGG_A
Cuts at: 3086 4353 6403 9339 3086
Size: 1267 2050 2936 3423
Fragments arranged by size:
3423 2936 2050 1267
BspHI T'CATG_A
Cuts at: 7565 9295 7565
Size: 1730 7946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2096 2813 3543 3774 6249 7031 844
Size: 1252 717 730 231 2475 782 3489
Fragments arranged by size:
3489 2475 1252 782 730 717 231
BsrBI CCG'CTC
Cuts at: 1394 1823 3293 4894 5004 5196 5455 7563 1394
Size: 429 1470 1601 110 192 259 2108 3507
Fragments arranged by size:
3507 2108 1601 1470 429 259 192 110
BsrDI GCAATG_nn'
Cuts at: 65 1248 2267 8171 8345 65
Size: 1183 1019 5904 174 1396
Fragments arranged by size:
5904 1396 1183 1019 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2683 4529 5175 8320 8507 1700
Size: 110 873 1846 646 3145 187 2869
Fragments arranged by size:
3145 2869 1846 873 646 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9676
BssSI C'ACGA_G
Cuts at: 5450 7736 8748 5450
Size: 2286 1012 6378
Fragments arranged by size:
6378 2286 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3940 4648 4751 794
Size: 3146 708 103 5719
Fragments arranged by size:
5719 3146 708 103
BstBI TT'CG_AA
Cuts at: 2007 8480 2007
Size: 6473 3203
BstEII G'GTnAC_C
Cuts at: 1184 4900 6229 6781 1184
Size: 3716 1329 552 4079
Fragments arranged by size:
4079 3716 1329 552
BstXI CCAn_nnnn'nTGG
Cuts at: 4842 5407 6217 4842
Size: 565 810 8301
Fragments arranged by size:
8301 810 565
BstYI r'GATC_y
Cuts at: 1972 2231 2526 2892 3122 3328 3343 3966 4137
Size: 259 295 366 230 206 15 623 171
Cuts at: 4137 6828 7006 7373 7758 7775 9227 9313 9325
Size: 2691 178 367 385 17 1452 86 12
Cuts at: 9325 9671 1972
Size: 346 1977
Fragments arranged by size:
2691 1977 1452 623 385 367 366 346
295 259 230 206 178 171 86 17
15 12
Bsu36I CC'TnA_GG
Cuts at: 6174 6174
Size: 9676
BtgI C'CryG_G
Cuts at: 513 1065 2022 2379 2898 3444 3462 3492 3807
Size: 552 957 357 519 546 18 30 315
Cuts at: 3807 4120 4288 5814 5967 6513 513
Size: 313 168 1526 153 546 3676
Fragments arranged by size:
3676 1526 957 552 546 546 519 357
315 313 168 153 30 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2649 487
Size: 683 56 1423 7514
Fragments arranged by size:
7514 1423 683 56
BtsI GCAGTG_nn'
Cuts at: 2143 2985 3364 3589 3710 4006 4752 6627 7192
Size: 842 379 225 121 296 746 1875 565
Cuts at: 7192 7986 8006 2143
Size: 794 20 3813
Fragments arranged by size:
3813 1875 842 794 746 565 379 296
225 121 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9676
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9641
DraI TTT'AAA
Cuts at: 6980 6980
Size: 9676
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2426 5290 8683 809
Size: 507 1110 2864 3393 1802
Fragments arranged by size:
3393 2864 1802 1110 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2901 3447 5456
Size: 54 1425 50 275 18 1071 546 2009
Cuts at: 5456 5573 5724 5964 5970 6750 6870 7123 8012
Size: 117 151 240 6 780 120 253 889
Cuts at: 8012 9641 8
Size: 1629 43
Fragments arranged by size:
2009 1629 1425 1071 889 780 546 275
253 240 151 120 117 54 50 43
18 6
EagI C'GGCC_G
Cuts at: 1537 1812 5724 7123 1537
Size: 275 3912 1399 4090
Fragments arranged by size:
4090 3912 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3029 3146 3151 3357 4475 5039 5323 5917
Size: 1676 117 5 206 1118 564 284 594
Cuts at: 5917 6767 7606 1353
Size: 850 839 3423
Fragments arranged by size:
3423 1676 1118 850 839 594 564 284
206 117 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4711 8263 8637 8783 387
Size: 1064 138 3122 3552 374 146 1280
Fragments arranged by size:
3552 3122 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2498 3111 4981 5109 5883 2498
Size: 613 1870 128 774 6291
Fragments arranged by size:
6291 1870 774 613 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 2294 2657 3618 3877 4043 4381
Size: 393 639 23 363 961 259 166 338
Cuts at: 4381 4525 5170 5182 5429 5780 5957 6515 7739
Size: 144 645 12 247 351 177 558 1224
Cuts at: 7739 8336 9123 9391 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1224 961 787 645 639 597 558
393 363 351 338 268 259 247 177
166 144 23 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2820 5642 6035 6812 6881 2820
Size: 2822 393 777 69 5615
Fragments arranged by size:
5615 2822 777 393 69
EcoO109I rG'GnC_Cy
Cuts at: 2566 2747 2829 3786 4013 4761 4801 4986 5260
Size: 181 82 957 227 748 40 185 274
Cuts at: 5260 5261 5508 5734 2566
Size: 1 247 226 6508
Fragments arranged by size:
6508 957 748 274 247 227 226 185
181 82 40 1
EcoRI G'AATT_C
Cuts at: 6985 7363 6985
Size: 378 9298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3955 3955
Size: 9676
FspI TGC'GCA
Cuts at: 3221 6496 6812 6914 8182 3221
Size: 3275 316 102 1268 4715
Fragments arranged by size:
4715 3275 1268 316 102
HaeII r_GCGC'y
Cuts at: 1678 2932 3004 3484 3621 3818 4034 4548 5146
Size: 1254 72 480 137 197 216 514 598
Cuts at: 5146 5636 6116 6264 8823 1678
Size: 490 480 148 2559 2531
Fragments arranged by size:
2559 2531 1254 598 514 490 480 480
216 197 148 137 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 4847 4879 5093
Size: 32 135 32 617 32 2626 32 214
Cuts at: 5093 5125 5828 5860 5867 5899 6122 6154 8317
Size: 32 703 32 7 32 223 32 2163
Cuts at: 8317 8349 8391 8423 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2626 2163 703 617 223 214 135
42 32 32 32 32 32 32 32
32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4338 7020 7266 669
Size: 988 281 2400 2682 246 3079
Fragments arranged by size:
3079 2682 2400 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9676
HpaI GTT'AAC
Cuts at: 7266 7266
Size: 9676
KasI G'GCGC_C
Cuts at: 2928 3480 3814 6112 2928
Size: 552 334 2298 6492
Fragments arranged by size:
6492 2298 552 334
KpnI G_GTAC'C
Cuts at: 2647 7001 2647
Size: 4354 5322
MfeI C'AATT_G
Cuts at: 7275 7275
Size: 9676
MluI A'CGCG_T
Cuts at: 9552 9552
Size: 9676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4545 4560 6231 8790 8974 1305
Size: 3240 15 1671 2559 184 2007
Fragments arranged by size:
3240 2559 2007 1671 184 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3449 6752 6872 10
Size: 54 1425 343 1617 3303 120 2814
Fragments arranged by size:
3303 2814 1617 1425 343 120 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 2990 7634 7993 8152 518
Size: 648 1009 815 4644 359 159 2042
Fragments arranged by size:
4644 2042 1009 815 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2309 2738 3240 3494 3774 3809 4495 5158
Size: 703 429 502 254 280 35 686 663
Cuts at: 5158 5437 5771 6590 7092 7769 8917 9162 1606
Size: 279 334 819 502 677 1148 245 2120
Fragments arranged by size:
2120 1148 819 703 686 677 663 502
502 429 334 280 279 254 245 35
NaeI GCC'GGC
Cuts at: 5177 5177
Size: 9676
NarI GG'CG_CC
Cuts at: 2929 3481 3815 6113 2929
Size: 552 334 2298 6492
Fragments arranged by size:
6492 2298 552 334
NcoI C'CATG_G
Cuts at: 513 1065 2022 4288 513
Size: 552 957 2266 5901
Fragments arranged by size:
5901 2266 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9676
NgoMIV G'CCGG_C
Cuts at: 5175 5175
Size: 9676
NheI G'CTAG_C
Cuts at: 1052 6670 1052
Size: 5618 4058
NotI GC'GGCC_GC
Cuts at: 7123 7123
Size: 9676
NspI r_CATG'y
Cuts at: 1132 1229 5705 6721 6913 7034 8579 1132
Size: 97 4476 1016 192 121 1545 2229
Fragments arranged by size:
4476 2229 1545 1016 192 121 97
PasI CC'CwG_GG
Cuts at: 1348 2063 3845 4842 5265 1348
Size: 715 1782 997 423 5759
Fragments arranged by size:
5759 1782 997 715 423
PciI A'CATG_T
Cuts at: 5701 8575 5701
Size: 2874 6802
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2380 3445 4804 1108
Size: 1272 1065 1359 5980
Fragments arranged by size:
5980 1359 1272 1065
PfoI T'CCnGG_A
Cuts at: 2673 3473 3719 4366 5757 2673
Size: 800 246 647 1391 6592
Fragments arranged by size:
6592 1391 800 647 246
PmeI GTTT'AAAC
Cuts at: 6980 6980
Size: 9676
PmlI CAC'GTG
Cuts at: 6397 6397
Size: 9676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 4692 4724 7745 7777 9258 9290 2879
Size: 32 1781 32 3021 32 1481 32 3265
Fragments arranged by size:
3265 3021 1781 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2566 2747 2829 3786 5508 2566
Size: 181 82 957 1722 6734
Fragments arranged by size:
6734 1722 957 181 82
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9676
PsiI TTA'TAA
Cuts at: 7246 7246
Size: 9676
PspOMI G'GGCC_C
Cuts at: 5260 5260
Size: 9676
PspXI vC'TCGA_Gb
Cuts at: 2817 5110 2817
Size: 2293 7383
PstI C_TGCA'G
Cuts at: 830 3432 3702 4956 5423 5745 6246 6634 7028
Size: 2602 270 1254 467 322 501 388 394
Cuts at: 7028 830
Size: 3478
Fragments arranged by size:
3478 2602 1254 501 467 394 388 322
270
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9676
PvuII CAG'CTG
Cuts at: 3240 3774 4495 5771 3240
Size: 534 721 1276 7145
Fragments arranged by size:
7145 1276 721 534
RsrII CG'GwC_CG
Cuts at: 4712 4712
Size: 9676
SacI G_AGCT'C
Cuts at: 2500 3113 4983 5111 5885 2500
Size: 613 1870 128 774 6291
Fragments arranged by size:
6291 1870 774 613 128
SacII CC_GC'GG
Cuts at: 3495 3810 3495
Size: 315 9361
SalI G'TCGA_C
Cuts at: 1936 4336 7018 1936
Size: 2400 2682 4594
Fragments arranged by size:
4594 2682 2400
SanDI GG'GwC_CC
Cuts at: 2747 2747
Size: 9676
SapI GCTCTTCn'nnn_
Cuts at: 3357 5323 3357
Size: 1966 7710
SbfI CC_TGCA'GG
Cuts at: 5745 6246 7028 5745
Size: 501 782 8393
Fragments arranged by size:
8393 782 501
ScaI AGT'ACT
Cuts at: 7924 9636 7924
Size: 1712 7964
SexAI A'CCwGG_T
Cuts at: 5675 6876 5675
Size: 1201 8475
SfcI C'TryA_G
Cuts at: 826 1045 3428 3698 4952 5419 5741 6242 6630
Size: 219 2383 270 1254 467 322 501 388
Cuts at: 6630 7024 8159 8840 9031 826
Size: 394 1135 681 191 1471
Fragments arranged by size:
2383 1471 1254 1135 681 501 467 394
388 322 270 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 5268 5732 5268
Size: 464 9212
SfoI GGC'GCC
Cuts at: 2930 3482 3816 6114 2930
Size: 552 334 2298 6492
Fragments arranged by size:
6492 2298 552 334
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9676
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9676
SmaI CCC'GGG
Cuts at: 1872 2834 4238 4246 6287 6412 7003 1872
Size: 962 1404 8 2041 125 591 4545
Fragments arranged by size:
4545 2041 1404 962 591 125 8
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2817 3970 5110 5845 6478 7780
Size: 197 926 874 1153 1140 735 633 1302
Cuts at: 7780 8681 8943 820
Size: 901 262 1553
Fragments arranged by size:
1553 1302 1153 1140 926 901 874 735
633 262 197
SnaBI TAC'GTA
Cuts at: 493 6973 7467 493
Size: 6480 494 2702
Fragments arranged by size:
6480 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9676
SphI G_CATG'C
Cuts at: 1132 7034 1132
Size: 5902 3774
SspI AAT'ATT
Cuts at: 5 52 4363 7600 5
Size: 47 4311 3237 2081
Fragments arranged by size:
4311 3237 2081 47
StuI AGG'CCT
Cuts at: 1517 3226 1517
Size: 1709 7967
StyI C'CwwG_G
Cuts at: 513 1065 2022 3733 4288 5163 5232 5688 6786
Size: 552 957 1711 555 875 69 456 1098
Cuts at: 6786 7115 513
Size: 329 3074
Fragments arranged by size:
3074 1711 1098 957 875 555 552 456
329 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2094 4951 4979 5123 5547 6059 6109
Size: 1255 514 2857 28 144 424 512 50
Cuts at: 6109 7703 7720 7873 8058 8503 325
Size: 1594 17 153 185 445 1498
Fragments arranged by size:
2857 1594 1498 1255 514 512 445 424
185 153 144 50 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6699 6795 6900
Size: 274 80 33 51 1443 4722 96 105
Cuts at: 6900 7922 9634 96
Size: 1022 1712 138
Fragments arranged by size:
4722 1712 1443 1022 274 138 105 96
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2587 2619 5458 5490 9636 9668 2587
Size: 32 2839 32 4146 32 2595
Fragments arranged by size:
4146 2839 2595 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5584 1316
Size: 4268 5408
XbaI T'CTAG_A
Cuts at: 7012 7012
Size: 9676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2965 5404 5965 6842 2965
Size: 2439 561 877 5799
Fragments arranged by size:
5799 2439 877 561
XhoI C'TCGA_G
Cuts at: 1943 2817 5110 1943
Size: 874 2293 6509
Fragments arranged by size:
6509 2293 874
XmaI C'CCGG_G
Cuts at: 1870 2832 4236 4244 6285 6410 7001 1870
Size: 962 1404 8 2041 125 591 4545
Fragments arranged by size:
4545 2041 1404 962 591 125 8
XmnI GAAnn'nnTTC
Cuts at: 7805 7805
Size: 9676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5286 7484 276
Size: 53 83 186 936 3752 2198 2468
Fragments arranged by size:
3752 2468 2198 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BstZ17I DraIII EcoRV
FseI FspAI NruI NsiI PacI PsrI SrfI SwaI