Restriction map for
pFN21ASDA1538
(Circular) (Six-base) MAPSORT of: pFN21ASDA1538.seq Check: 3704 from: 1 to: 7768
pFN21ASDA1538 7768 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3412 4505 3412
Size: 1093 6675
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2118 5578 278
Size: 53 83 186 936 582 3460 2468
Fragments arranged by size:
3460 2468 936 582 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 4626 5111 1937
Size: 879 1810 485 4594
Fragments arranged by size:
4594 1810 879 485
Acc65I G'GTAC_C
Cuts at: 2578 3114 5089 2578
Size: 536 1975 5257
Fragments arranged by size:
5257 1975 536
AclI AA'CG_TT
Cuts at: 1563 5895 6268 1563
Size: 4332 373 3063
Fragments arranged by size:
4332 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2170 2190 2531 2637 3014 3182 4819 5831
Size: 538 20 341 106 377 168 1637 1012
Cuts at: 5831 7215 7483 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1637 1384 1012 538 377 341 268
168 106 20
AfeI AGC'GCT
Cuts at: 4441 4441
Size: 7768
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7571
AflIII A'CryG_T
Cuts at: 1203 6667 7644 1203
Size: 5464 977 1327
Fragments arranged by size:
5464 1327 977
AgeI A'CCGG_T
Cuts at: 6599 6599
Size: 7768
AhdI GACnn_n'nnGTC
Cuts at: 6497 6497
Size: 7768
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7768
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4103 4398 1184
Size: 2919 295 4554
Fragments arranged by size:
4554 2919 295
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2079 2111 2079
Size: 32 7736
AlwNI CAG_nnn'CTG
Cuts at: 2234 2423 2554 3416 3785 3884 4126 5511 7083
Size: 189 131 862 369 99 242 1385 1572
Cuts at: 7083 2234
Size: 2919
Fragments arranged by size:
2919 1572 1385 862 369 242 189 131
99
ApaI G_GGCC'C
Cuts at: 2637 2643 2909 3797 4903 2637
Size: 6 266 888 1106 5502
Fragments arranged by size:
5502 1106 888 266 6
ApaLI G'TGCA_C
Cuts at: 5825 6981 5825
Size: 1156 6612
ApoI r'AATT_y
Cuts at: 1493 4643 5077 5303 5455 7564 1493
Size: 3150 434 226 152 2109 1697
Fragments arranged by size:
3150 2109 1697 434 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6322 160
Size: 872 5290 1606
Fragments arranged by size:
5290 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7768
AvaI C'yCGr_G
Cuts at: 1870 1943 2391 2636 2909 3196 3562 4092 4287
Size: 73 448 245 273 287 366 530 195
Cuts at: 4287 4397 4454 5093 1870
Size: 110 57 639 4545
Fragments arranged by size:
4545 639 530 448 366 287 273 245
195 110 73 57
BamHI G'GATC_C
Cuts at: 5098 5465 5098
Size: 367 7401
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2240 2523 2578 3114 4064
Size: 325 217 627 453 283 55 536 950
Cuts at: 4064 4197 4403 4445 5089 6544 618
Size: 133 206 42 644 1455 1842
Fragments arranged by size:
1842 1455 950 644 627 536 453 325
283 217 206 133 55 42
BanII G_rGCy'C
Cuts at: 1391 2637 2643 2909 3325 3797 4169 4277 4596
Size: 1246 6 266 416 472 372 108 319
Cuts at: 4596 4785 4903 5087 1391
Size: 189 118 184 4072
Fragments arranged by size:
4072 1246 472 416 372 319 266 189
184 118 108 6
BbeI G_GCGC'C
Cuts at: 2244 2527 2244
Size: 283 7485
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 3170 3224 928
Size: 971 393 878 54 5472
Fragments arranged by size:
5472 971 878 393 54
BbvCI CC'TCA_GC
Cuts at: 2676 2872 4536 2676
Size: 196 1664 5908
Fragments arranged by size:
5908 1664 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5959 5993 1903
Size: 34 4022 34 3678
Fragments arranged by size:
4022 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5660 6870 5660
Size: 1210 6558
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7768
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2532 2550 2600
Size: 107 122 71 631 605 860 18 50
Cuts at: 2600 2716 3329 3368 3394 6379 136
Size: 116 613 39 26 2985 1525
Fragments arranged by size:
2985 1525 860 631 613 605 122 116
107 71 50 39 26 18
BglII A'GATC_T
Cuts at: 7763 7763
Size: 7768
BlpI GC'TnA_GC
Cuts at: 3905 4389 5185 3905
Size: 484 796 6488
Fragments arranged by size:
6488 796 484
Bme1580I G_kGCm'C
Cuts at: 1790 2637 2643 2909 3797 4183 4219 4406 4903
Size: 847 6 266 888 386 36 187 497
Cuts at: 4903 5829 6985 1790
Size: 926 1156 2573
Fragments arranged by size:
2573 1156 926 888 847 497 386 266
187 36 6
BmgBI CAC'GTC
Cuts at: 1318 4178 1318
Size: 2860 4908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2244 2728 2779
Size: 416 53 83 399 24 827 484 51
Cuts at: 2779 4592 5499 6457 442
Size: 1813 907 958 1753
Fragments arranged by size:
1813 1753 958 907 827 484 416 399
83 53 51 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7768
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4573 4573
Size: 7768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2346 2420 4033 4071 4080 4547 6428 1239
Size: 1107 74 1613 38 9 467 1881 2579
Fragments arranged by size:
2579 1881 1613 1107 467 74 38 9
Bpu10I CC'TnA_GC
Cuts at: 2676 2872 3603 4038 4361 4536 4779 2676
Size: 196 731 435 323 175 243 5665
Fragments arranged by size:
5665 731 435 323 243 196 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3242 4929 5893 6758 7056 3242
Size: 1687 964 865 298 3954
Fragments arranged by size:
3954 1687 964 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3131 4544 6431 882
Size: 617 1632 1413 1887 2219
Fragments arranged by size:
2219 1887 1632 1413 617
BsaAI yAC'GTr
Cuts at: 493 1204 2917 2941 2977 5065 5559 493
Size: 711 1713 24 36 2088 494 2702
Fragments arranged by size:
2702 2088 1713 711 494 36 24
BsaBI GATnn'nnATC
Cuts at: 7762 7762
Size: 7768
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 2241 2524 2868
Size: 53 83 186 936 582 126 283 344
Cuts at: 2868 5575 5957 6592 275
Size: 2707 382 635 1451
Fragments arranged by size:
2707 1451 936 635 582 382 344 283
186 126 83 53
BsaWI w'CCGG_w
Cuts at: 4737 6201 6599 6873 7020 7431 4737
Size: 1464 398 274 147 411 5074
Fragments arranged by size:
5074 1464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2079 2109 3098 3128 4960
Size: 30 165 30 676 30 989 30 1832
Cuts at: 4960 4975 4990 5005 1178
Size: 15 15 15 3941
Fragments arranged by size:
3941 1832 989 676 165 30 30 30
30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2272 2275 2278 2308 3585 3701 3866 3929
Size: 1086 3 3 30 1277 116 165 63
Cuts at: 3929 3938 3947 3959 3962 3974 3977 3980 3983
Size: 9 9 12 3 12 3 3 3
Cuts at: 3983 3992 4150 4486 4604 1186
Size: 9 158 336 118 4350
Fragments arranged by size:
4350 1277 1086 336 165 158 118 116
63 30 12 12 9 9 9 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2416 2590 2651 2824 2831 3451 3628 3646 4140
Size: 174 61 173 7 620 177 18 494
Cuts at: 4140 4153 4168 4806 6971 7467 2416
Size: 13 15 638 2165 496 2717
Fragments arranged by size:
2717 2165 638 620 496 494 177 174
173 61 18 15 13 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4658 1163
Size: 573 2922 4273
Fragments arranged by size:
4273 2922 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4161 4672 5218 5979 7007 1540
Size: 275 186 2160 511 546 761 1028 2301
Fragments arranged by size:
2301 2160 1028 761 546 511 275 186
BsiHKAI G_wGCw'C
Cuts at: 2705 2916 4529 5829 5914 6985 2705
Size: 211 1613 1300 85 1071 3488
Fragments arranged by size:
3488 1613 1300 1071 211 85
BsmI GAATG_Cn'
Cuts at: 5279 5372 5279
Size: 93 7675
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2637 2643 2705 2909 2916 3325 3797
Size: 399 847 6 62 204 7 409 472
Cuts at: 3797 4169 4183 4219 4277 4406 4529 4596 4785
Size: 372 14 36 58 129 123 67 189
Cuts at: 4785 4903 5087 5829 5914 6985 1391
Size: 118 184 742 85 1071 2174
Fragments arranged by size:
2174 1071 847 742 472 409 399 372
204 189 184 129 123 118 85 67
62 58 36 14 7 6
BspEI T'CCGG_A
Cuts at: 7431 7431
Size: 7768
BspHI T'CATG_A
Cuts at: 5657 7387 5657
Size: 1730 6038
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3102 3412 3897 4328 4505 5123 844
Size: 2258 310 485 431 177 618 3489
Fragments arranged by size:
3489 2258 618 485 431 310 177
BsrBI CCG'CTC
Cuts at: 1394 1823 3909 4192 4393 4792 4850 5655 1394
Size: 429 2086 283 201 399 58 805 3507
Fragments arranged by size:
3507 2086 805 429 399 283 201 58
BsrDI GCAATG_nn'
Cuts at: 65 1248 3013 3378 4419 6263 6437 65
Size: 1183 1765 365 1041 1844 174 1396
Fragments arranged by size:
1844 1765 1396 1183 1041 365 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3358 4101 6412 6599 1700
Size: 110 1548 743 2311 187 2869
Fragments arranged by size:
2869 2311 1548 743 187 110
BsrGI T'GTAC_A
Cuts at: 96 3010 96
Size: 2914 4854
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7768
BssSI C'ACGA_G
Cuts at: 3016 5828 6840 3016
Size: 2812 1012 3944
Fragments arranged by size:
3944 2812 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4958 794
Size: 4164 3604
BstBI TT'CG_AA
Cuts at: 2007 6572 2007
Size: 4565 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7768
BstXI CCAn_nnnn'nTGG
Cuts at: 3745 4113 3745
Size: 368 7400
BstYI r'GATC_y
Cuts at: 1972 5098 5465 5850 5867 7319 7405 7417 7763
Size: 3126 367 385 17 1452 86 12 346
Cuts at: 7763 1972
Size: 1977
Fragments arranged by size:
3126 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 4627 4627
Size: 7768
Bsu36I CC'TnA_GG
Cuts at: 5007 5007
Size: 7768
BtgI C'CryG_G
Cuts at: 513 1065 2098 2598 3184 3472 3669 3681 3707
Size: 552 1033 500 586 288 197 12 26
Cuts at: 3707 4264 4369 513
Size: 557 105 3912
Fragments arranged by size:
3912 1033 586 557 552 500 288 197
105 26 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5067 487
Size: 683 56 3841 3188
Fragments arranged by size:
3841 3188 683 56
BtsI GCAGTG_nn'
Cuts at: 2468 2705 3493 3552 4087 4327 4527 4941 5284
Size: 237 788 59 535 240 200 414 343
Cuts at: 5284 6078 6098 2468
Size: 794 20 4138
Fragments arranged by size:
4138 794 788 535 414 343 240 237
200 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7768
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4638 4673 529
Size: 35 4074 35 3624
Fragments arranged by size:
4074 3624 35 35
DraI TTT'AAA
Cuts at: 5072 5072
Size: 7768
DraIII CAC_nnn'GTG
Cuts at: 2920 2920
Size: 7768
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2050 6775 809
Size: 507 734 4725 1802
Fragments arranged by size:
4725 1802 734 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2601 2935 3510
Size: 54 1425 50 275 18 771 334 575
Cuts at: 3510 3672 3787 4103 4158 4669 4845 5215 6104
Size: 162 115 316 55 511 176 370 889
Cuts at: 6104 7733 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 771 575 511 370 334
316 275 176 162 115 55 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4158 4669 5215 1537
Size: 275 2346 511 546 4090
Fragments arranged by size:
4090 2346 546 511 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2111 2281 2296 2314 2347 2353 2613 2990
Size: 758 170 15 18 33 6 260 377
Cuts at: 2990 3936 3951 4992 5698 1353
Size: 946 15 1041 706 3423
Fragments arranged by size:
3423 1041 946 758 706 377 260 170
33 18 15 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3037 3083 6355 6729 6875 387
Size: 1064 138 1448 46 3272 374 146 1280
Fragments arranged by size:
3272 1448 1280 1064 374 146 138 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2170 2190 2346 2420 2531 2637 3014
Size: 393 538 20 156 74 111 106 377
Cuts at: 3014 3182 4033 4071 4080 4547 4819 5831 6428
Size: 168 851 38 9 467 272 1012 597
Cuts at: 6428 7215 7483 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1012 851 787 597 538 467 393
377 272 268 168 156 111 106 74
38 20 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2558 2558
Size: 7768
EcoO109I rG'GnC_Cy
Cuts at: 2194 2395 2551 2686 2905 2906 3525 3770 3793
Size: 201 156 135 219 1 619 245 23
Cuts at: 3793 3794 4017 4302 4567 4899 4900 2194
Size: 1 223 285 265 332 1 5062
Fragments arranged by size:
5062 619 332 285 265 245 223 219
201 156 135 23 1 1 1
EcoRI G'AATT_C
Cuts at: 5077 5455 5077
Size: 378 7390
FspI TGC'GCA
Cuts at: 6274 6274
Size: 7768
HaeII r_GCGC'y
Cuts at: 1678 2244 2460 2527 2841 3871 4443 6915 1678
Size: 566 216 67 314 1030 572 2472 2531
Fragments arranged by size:
2531 2472 1030 572 566 314 216 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3968 4000 6409 6441 6483
Size: 32 135 32 2396 32 2409 32 42
Cuts at: 6483 6515 1373
Size: 32 2626
Fragments arranged by size:
2626 2409 2396 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5112 5358 669
Size: 988 281 3174 246 3079
Fragments arranged by size:
3174 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2746 4542 748
Size: 1998 1796 3974
Fragments arranged by size:
3974 1998 1796
HpaI GTT'AAC
Cuts at: 5358 5358
Size: 7768
KasI G'GCGC_C
Cuts at: 2240 2523 2240
Size: 283 7485
KpnI G_GTAC'C
Cuts at: 2582 3118 5093 2582
Size: 536 1975 5257
Fragments arranged by size:
5257 1975 536
MfeI C'AATT_G
Cuts at: 5367 5367
Size: 7768
MluI A'CGCG_T
Cuts at: 7644 7644
Size: 7768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3368 4094 6882 7066 1305
Size: 2063 726 2788 184 2007
Fragments arranged by size:
2788 2063 2007 726 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2603 3674 10
Size: 54 1425 343 771 1071 4104
Fragments arranged by size:
4104 1425 1071 771 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3006 5726 6085 6244 518
Size: 648 1840 2720 359 159 2042
Fragments arranged by size:
2720 2042 1840 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2270 2423 2498 2651 3656 3805 4157
Size: 486 178 153 75 153 1005 149 352
Cuts at: 4157 4172 4355 4506 5184 5861 7009 7254 1606
Size: 15 183 151 678 677 1148 245 2120
Fragments arranged by size:
2120 1148 1005 678 677 486 352 245
183 178 153 153 151 149 75 15
NaeI GCC'GGC
Cuts at: 4103 4103
Size: 7768
NarI GG'CG_CC
Cuts at: 2241 2524 2241
Size: 283 7485
NcoI C'CATG_G
Cuts at: 513 1065 2598 3472 3669 513
Size: 552 1533 874 197 4612
Fragments arranged by size:
4612 1533 874 552 197
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7768
NgoMIV G'CCGG_C
Cuts at: 4101 4101
Size: 7768
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7768
NotI GC'GGCC_GC
Cuts at: 5215 5215
Size: 7768
NspI r_CATG'y
Cuts at: 1132 1229 4385 5126 6671 1132
Size: 97 3156 741 1545 2229
Fragments arranged by size:
3156 2229 1545 741 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7768
PciI A'CATG_T
Cuts at: 6667 6667
Size: 7768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2234 3416 3713 1108
Size: 1126 1182 297 5163
Fragments arranged by size:
5163 1182 1126 297
PfoI T'CCnGG_A
Cuts at: 2397 4563 2397
Size: 2166 5602
PmeI GTTT'AAAC
Cuts at: 5072 5072
Size: 7768
PmlI CAC'GTG
Cuts at: 2917 2941 2917
Size: 24 7744
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2079 2111 5837 5869 7350 7382 2079
Size: 32 3726 32 1481 32 2465
Fragments arranged by size:
3726 2465 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 3525 3770 4567 2395
Size: 1130 245 797 5596
Fragments arranged by size:
5596 1130 797 245
PshAI GACnn'nnGTC
Cuts at: 1613 3143 3891 1613
Size: 1530 748 5490
Fragments arranged by size:
5490 1530 748
PsiI TTA'TAA
Cuts at: 5338 5338
Size: 7768
PspOMI G'GGCC_C
Cuts at: 2633 2639 2905 3793 4899 2633
Size: 6 266 888 1106 5502
Fragments arranged by size:
5502 1106 888 266 6
PstI C_TGCA'G
Cuts at: 830 2038 2772 2860 3613 3661 3810 5120 830
Size: 1208 734 88 753 48 149 1310 3478
Fragments arranged by size:
3478 1310 1208 753 734 149 88 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7768
PvuII CAG'CTG
Cuts at: 2270 2423 2498 2651 3805 4355 2270
Size: 153 75 153 1154 550 5683
Fragments arranged by size:
5683 1154 550 153 153 75
SalI G'TCGA_C
Cuts at: 1936 5110 1936
Size: 3174 4594
SanDI GG'GwC_CC
Cuts at: 3525 3770 3525
Size: 245 7523
SbfI CC_TGCA'GG
Cuts at: 2860 5120 2860
Size: 2260 5508
ScaI AGT'ACT
Cuts at: 6016 7728 6016
Size: 1712 6056
SfcI C'TryA_G
Cuts at: 826 1045 2034 2452 2499 2768 2817 2856 3310
Size: 219 989 418 47 269 49 39 454
Cuts at: 3310 3609 3657 3806 5116 6251 6932 7123 826
Size: 299 48 149 1310 1135 681 191 1471
Fragments arranged by size:
1471 1310 1135 989 681 454 418 299
269 219 191 149 49 48 47 39
SfoI GGC'GCC
Cuts at: 2242 2525 2242
Size: 283 7485
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7768
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7768
SmaI CCC'GGG
Cuts at: 1872 2393 2638 3198 4289 4399 4456 5095 1872
Size: 521 245 560 1091 110 57 639 4545
Fragments arranged by size:
4545 1091 639 560 521 245 110 57
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3257 4944 5872 6773 7035 820
Size: 197 926 1314 1687 928 901 262 1553
Fragments arranged by size:
1687 1553 1314 928 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5065 5559 493
Size: 4572 494 2702
Fragments arranged by size:
4572 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7768
SphI G_CATG'C
Cuts at: 1132 4385 5126 1132
Size: 3253 741 3774
Fragments arranged by size:
3774 3253 741
SrfI GCCC'GGGC
Cuts at: 2638 3198 2638
Size: 560 7208
SspI AAT'ATT
Cuts at: 5 52 5692 5
Size: 47 5640 2081
Fragments arranged by size:
5640 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7768
StyI C'CwwG_G
Cuts at: 513 1065 2598 3472 3669 3693 4335 4431 5207
Size: 552 1533 874 197 24 642 96 776
Cuts at: 5207 513
Size: 3074
Fragments arranged by size:
3074 1533 874 776 642 552 197 96
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2263 3238 3465 3586 3634 5795 5812
Size: 1255 683 975 227 121 48 2161 17
Cuts at: 5812 5965 6150 6595 325
Size: 153 185 445 1498
Fragments arranged by size:
2161 1498 1255 975 683 445 227 185
153 121 48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3010 6014 7726
Size: 274 80 33 51 1443 1033 3004 1712
Cuts at: 7726 96
Size: 138
Fragments arranged by size:
3004 1712 1443 1033 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2379 2411 7728 7760 2379
Size: 32 5317 32 2387
Fragments arranged by size:
5317 2387 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2983 1316
Size: 1667 6101
XbaI T'CTAG_A
Cuts at: 5104 5104
Size: 7768
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2655 2655
Size: 7768
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7768
XmaI C'CCGG_G
Cuts at: 1870 2391 2636 3196 4287 4397 4454 5093 1870
Size: 521 245 560 1091 110 57 639 4545
Fragments arranged by size:
4545 1091 639 560 521 245 110 57
XmnI GAAnn'nnTTC
Cuts at: 5897 5897
Size: 7768
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2116 5576 276
Size: 53 83 186 936 582 3460 2468
Fragments arranged by size:
3460 2468 936 582 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SwaI