Restriction map for
pFN21ASDA1518
(Circular) (Six-base) MAPSORT of: pFN21ASDA1518.seq Check: 209 from: 1 to: 7282
pFN21ASDA1518 7282 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2092 2626 2092
Size: 534 6748
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2270 5092 278
Size: 53 83 186 936 734 2822 2468
Fragments arranged by size:
2822 2468 936 734 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4625 1937
Size: 2688 4594
Acc65I G'GTAC_C
Cuts at: 4603 4603
Size: 7282
AclI AA'CG_TT
Cuts at: 1563 5409 5782 1563
Size: 3846 373 3063
Fragments arranged by size:
3846 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4175 4181 5345 6729 6997 1632
Size: 2543 6 1164 1384 268 1917
Fragments arranged by size:
2543 1917 1384 1164 268 6
AflII C'TTAA_G
Cuts at: 820 1017 2711 820
Size: 197 1694 5391
Fragments arranged by size:
5391 1694 197
AflIII A'CryG_T
Cuts at: 1203 2203 6181 7158 1203
Size: 1000 3978 977 1327
Fragments arranged by size:
3978 1327 1000 977
AgeI A'CCGG_T
Cuts at: 2551 6113 2551
Size: 3562 3720
AhdI GACnn_n'nnGTC
Cuts at: 6011 6011
Size: 7282
AleI CACnn'nnGTG
Cuts at: 1166 2552 3887 1166
Size: 1386 1335 4561
Fragments arranged by size:
4561 1386 1335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2061 1184
Size: 877 6405
AlwNI CAG_nnn'CTG
Cuts at: 2035 2929 3062 3091 4078 5025 6597 2035
Size: 894 133 29 987 947 1572 2720
Fragments arranged by size:
2720 1572 987 947 894 133 29
ApaI G_GGCC'C
Cuts at: 3085 3121 3447 3085
Size: 36 326 6920
Fragments arranged by size:
6920 326 36
ApaLI G'TGCA_C
Cuts at: 5339 6495 5339
Size: 1156 6126
ApoI r'AATT_y
Cuts at: 1493 4591 4817 4969 7078 1493
Size: 3098 226 152 2109 1697
Fragments arranged by size:
3098 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5836 160
Size: 872 4804 1606
Fragments arranged by size:
4804 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
AvaI C'yCGr_G
Cuts at: 1870 1943 2426 3310 3717 3935 4607 1870
Size: 73 483 884 407 218 672 4545
Fragments arranged by size:
4545 884 672 483 407 218 73
BamHI G'GATC_C
Cuts at: 4612 4979 4612
Size: 367 6915
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2554 2571 2603 2825 3065
Size: 325 217 627 767 17 32 222 240
Cuts at: 3065 3434 3951 4603 6058 618
Size: 369 517 652 1455 1842
Fragments arranged by size:
1842 1455 767 652 627 517 369 325
240 222 217 32 17
BanII G_rGCy'C
Cuts at: 1391 2237 2462 2766 3085 3121 3149 3378 3393
Size: 846 225 304 319 36 28 229 15
Cuts at: 3393 3447 4553 4601 1391
Size: 54 1106 48 4072
Fragments arranged by size:
4072 1106 846 319 304 229 225 54
48 36 28 15
BbeI G_GCGC'C
Cuts at: 3069 3069
Size: 7282
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3291 4169 928
Size: 971 1392 878 4041
Fragments arranged by size:
4041 1392 971 878
BbvCI CC'TCA_GC
Cuts at: 3113 3744 3113
Size: 631 6651
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5473 5507 1903
Size: 34 3536 34 3678
Fragments arranged by size:
3678 3536 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2998 5174 6384 2998
Size: 2176 1210 3896
Fragments arranged by size:
3896 2176 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7282
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2951 3136 3397
Size: 107 122 71 631 605 1279 185 261
Cuts at: 3397 3818 4232 4298 4307 5893 136
Size: 421 414 66 9 1586 1525
Fragments arranged by size:
1586 1525 1279 631 605 421 414 261
185 122 107 71 66 9
BglII A'GATC_T
Cuts at: 7277 7277
Size: 7282
BlpI GC'TnA_GC
Cuts at: 3791 4699 3791
Size: 908 6374
Bme1580I G_kGCm'C
Cuts at: 1790 2574 2608 2828 3085 3121 3191 3198 3366
Size: 784 34 220 257 36 70 7 168
Cuts at: 3366 3437 3447 4407 5343 6499 1790
Size: 71 10 960 936 1156 2573
Fragments arranged by size:
2573 1156 960 936 784 257 220 168
71 70 36 34 10 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2466 2556 2792
Size: 416 53 83 399 24 1049 90 236
Cuts at: 2792 3185 5013 5971 442
Size: 393 1828 958 1753
Fragments arranged by size:
1828 1753 1049 958 416 399 393 236
90 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7282
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3343 3601 4464 3343
Size: 258 863 6161
Fragments arranged by size:
6161 863 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 2642 2648 2663 2828 2918 3113 3503
Size: 1145 258 6 15 165 90 195 390
Cuts at: 3503 3550 5942 1239
Size: 47 2392 2579
Fragments arranged by size:
2579 2392 1145 390 258 195 165 90
47 15 6
Bpu10I CC'TnA_GC
Cuts at: 2231 2940 3113 3662 3744 2231
Size: 709 173 549 82 5769
Fragments arranged by size:
5769 709 549 173 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 2894 5407 6272 6570 2147
Size: 747 2513 865 298 2859
Fragments arranged by size:
2859 2513 865 747 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2120 2360 2804 2894 5945 882
Size: 617 621 240 444 90 3051 2219
Fragments arranged by size:
3051 2219 621 617 444 240 90
BsaAI yAC'GTr
Cuts at: 493 1204 2041 2170 2584 3997 4579 5073 493
Size: 711 837 129 414 1413 582 494 2702
Fragments arranged by size:
2702 1413 837 711 582 494 414 129
BsaBI GATnn'nnATC
Cuts at: 3239 7276 3239
Size: 4037 3245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2267 3066 5089 5471
Size: 53 83 186 936 734 799 2023 382
Cuts at: 5471 6106 275
Size: 635 1451
Fragments arranged by size:
2023 1451 936 799 734 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2551 3507 5715 6113 6387 6534 6945 2551
Size: 956 2208 398 274 147 411 2888
Fragments arranged by size:
2888 2208 956 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3170 3200 1178
Size: 30 165 30 1767 30 5260
Fragments arranged by size:
5260 1767 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2645 3437 3497 3526 3785 3788 1186
Size: 1459 792 60 29 259 3 4680
Fragments arranged by size:
4680 1459 792 259 60 29 3
BseYI C'CCAG_C
Cuts at: 2071 2904 2961 3657 4406 6485 6981 2071
Size: 833 57 696 749 2079 496 2372
Fragments arranged by size:
2372 2079 833 749 696 496 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2021 2234 4019 4091 1163
Size: 573 285 213 1785 72 4354
Fragments arranged by size:
4354 1785 573 285 213 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2335 2481 4732 5493 6521 1540
Size: 275 186 334 146 2251 761 1028 2301
Fragments arranged by size:
2301 2251 1028 761 334 275 186 146
BsiHKAI G_wGCw'C
Cuts at: 2237 2288 2362 2658 2679 2766 3353 4368 4445
Size: 51 74 296 21 87 587 1015 77
Cuts at: 4445 4523 5343 5428 6499 2237
Size: 78 820 85 1071 3020
Fragments arranged by size:
3020 1071 1015 820 587 296 87 85
78 77 74 51 21
BsmI GAATG_Cn'
Cuts at: 4793 4886 4793
Size: 93 7189
BsmBI CGTCTCn'nnnn_
Cuts at: 2429 2429
Size: 7282
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2288 2362 2462 2574 2608 2658
Size: 399 447 51 74 100 112 34 50
Cuts at: 2658 2679 2766 2828 3085 3121 3149 3191 3198
Size: 21 87 62 257 36 28 42 7
Cuts at: 3198 3353 3366 3378 3393 3437 3447 4368 4407
Size: 155 13 12 15 44 10 921 39
Cuts at: 4407 4445 4523 4553 4601 5343 5428 6499 1391
Size: 38 78 30 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 921 742 447 399 257 155
112 100 87 85 78 74 62 51
50 48 44 42 39 38 36 34
30 28 21 15 13 12 10 7
BspEI T'CCGG_A
Cuts at: 6945 6945
Size: 7282
BspHI T'CATG_A
Cuts at: 3468 5171 6901 3468
Size: 1703 1730 3849
Fragments arranged by size:
3849 1730 1703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2092 2626 4637 844
Size: 1248 534 2011 3489
Fragments arranged by size:
3489 2011 1248 534
BsrBI CCG'CTC
Cuts at: 1394 1823 3862 5169 1394
Size: 429 2039 1307 3507
Fragments arranged by size:
3507 2039 1307 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3132 5777 5951 65
Size: 1183 1884 2645 174 1396
Fragments arranged by size:
2645 1884 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2450 2480 2551 3437 3696 5926 6113
Size: 110 640 30 71 886 259 2230 187
Cuts at: 6113 1700
Size: 2869
Fragments arranged by size:
2869 2230 886 640 259 187 110 71
30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7282
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7282
BssSI C'ACGA_G
Cuts at: 5342 6354 5342
Size: 1012 6270
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2929 4078 794
Size: 2135 1149 3998
Fragments arranged by size:
3998 2135 1149
BstBI TT'CG_AA
Cuts at: 2007 6086 2007
Size: 4079 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7282
BstXI CCAn_nnnn'nTGG
Cuts at: 2455 2455
Size: 7282
BstYI r'GATC_y
Cuts at: 1972 4612 4979 5364 5381 6833 6919 6931 7277
Size: 2640 367 385 17 1452 86 12 346
Cuts at: 7277 1972
Size: 1977
Fragments arranged by size:
2640 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2121 2245 2335 2371 2387 2528
Size: 552 957 99 124 90 36 16 141
Cuts at: 2528 3029 3498 4065 4267 4341 513
Size: 501 469 567 202 74 3454
Fragments arranged by size:
3454 957 567 552 501 469 202 141
124 99 90 74 36 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3273 3991 4449 487
Size: 683 56 2047 718 458 3320
Fragments arranged by size:
3320 2047 718 683 458 56
BtsI GCAGTG_nn'
Cuts at: 2217 3738 4798 5592 5612 2217
Size: 1521 1060 794 20 3887
Fragments arranged by size:
3887 1521 1060 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2278 2313 529
Size: 35 1714 35 5498
Fragments arranged by size:
5498 1714 35 35
DraI TTT'AAA
Cuts at: 4586 4586
Size: 7282
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2993 3998 4091 6289 809
Size: 507 1677 1005 93 2198 1802
Fragments arranged by size:
2198 1802 1677 1005 507 93
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2332 2439 2478
Size: 54 1425 50 275 18 502 107 39
Cuts at: 2478 2975 3048 3645 3684 4152 4257 4369 4398
Size: 497 73 597 39 468 105 112 29
Cuts at: 4398 4729 5618 7247 8
Size: 331 889 1629 43
Fragments arranged by size:
1629 1425 889 597 502 497 468 331
275 112 107 105 73 54 50 43
39 39 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2332 2478 4729 1537
Size: 275 520 146 2251 4090
Fragments arranged by size:
4090 2251 520 275 146
EarI CTCTTCn'nnn_
Cuts at: 1353 3341 4202 4566 5212 1353
Size: 1988 861 364 646 3423
Fragments arranged by size:
3423 1988 861 646 364
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5869 6243 6389 387
Size: 1064 138 4280 374 146 1280
Fragments arranged by size:
4280 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2235 2764 2235
Size: 529 6753
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2384 2642 2648 2663 2828 2918 3113
Size: 393 752 258 6 15 165 90 195
Cuts at: 3113 3503 3550 4175 4181 5345 5942 6729 6997
Size: 390 47 625 6 1164 597 787 268
Cuts at: 6997 1239
Size: 1524
Fragments arranged by size:
1524 1164 787 752 625 597 393 390
268 258 195 165 90 47 15 6
6
EcoO109I rG'GnC_Cy
Cuts at: 2032 2061 2101 2794 3117 3118 3444 3613 2032
Size: 29 40 693 323 1 326 169 5701
Fragments arranged by size:
5701 693 326 323 169 40 29 1
EcoRI G'AATT_C
Cuts at: 4591 4969 4591
Size: 378 6904
FspI TGC'GCA
Cuts at: 5788 5788
Size: 7282
HaeII r_GCGC'y
Cuts at: 1678 2221 2921 3069 6429 1678
Size: 543 700 148 3360 2531
Fragments arranged by size:
3360 2531 700 543 148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3539 3571 3737 3769 3791
Size: 32 135 32 1967 32 166 32 22
Cuts at: 3791 3823 4322 4354 4472 4504 5923 5955 5997
Size: 32 499 32 118 32 1419 32 42
Cuts at: 5997 6029 1373
Size: 32 2626
Fragments arranged by size:
2626 1967 1419 499 166 135 118 42
32 32 32 32 32 32 32 32
32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3535 4003 4318 4626 4872 669
Size: 988 281 1597 468 315 308 246 3079
Fragments arranged by size:
3079 1597 988 468 315 308 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7282
HpaI GTT'AAC
Cuts at: 4872 4872
Size: 7282
KasI G'GCGC_C
Cuts at: 3065 3065
Size: 7282
KpnI G_GTAC'C
Cuts at: 4607 4607
Size: 7282
MfeI C'AATT_G
Cuts at: 4881 4881
Size: 7282
MluI A'CGCG_T
Cuts at: 2203 7158 2203
Size: 4955 2327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6396 6580 1305
Size: 5091 184 2007
Fragments arranged by size:
5091 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2977 3050 4154 10
Size: 54 1425 343 1145 73 1104 3138
Fragments arranged by size:
3138 1425 1145 1104 343 73 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 3887 5240 5599 5758 518
Size: 648 1386 1335 1353 359 159 2042
Fragments arranged by size:
2042 1386 1353 1335 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2373 2389 2626 2744 3062 3079 3200 3683
Size: 767 16 237 118 318 17 121 483
Cuts at: 3683 3712 4078 4089 4410 4698 5375 6523 6768
Size: 29 366 11 321 288 677 1148 245
Cuts at: 6768 1606
Size: 2120
Fragments arranged by size:
2120 1148 767 677 483 366 321 318
288 245 237 121 118 29 17 16
11
NaeI GCC'GGC
Cuts at: 2482 3698 2482
Size: 1216 6066
NarI GG'CG_CC
Cuts at: 3066 3066
Size: 7282
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5773
Fragments arranged by size:
5773 957 552
NdeI CA'TA_TG
Cuts at: 387 3222 387
Size: 2835 4447
NgoMIV G'CCGG_C
Cuts at: 2480 3696 2480
Size: 1216 6066
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7282
NotI GC'GGCC_GC
Cuts at: 4729 4729
Size: 7282
NruI TCG'CGA
Cuts at: 4434 4434
Size: 7282
NspI r_CATG'y
Cuts at: 1132 1229 2857 4640 6185 1132
Size: 97 1628 1783 1545 2229
Fragments arranged by size:
2229 1783 1628 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2057 1348
Size: 709 6573
PciI A'CATG_T
Cuts at: 6181 6181
Size: 7282
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3091 3172 3526 1108
Size: 1983 81 354 4864
Fragments arranged by size:
4864 1983 354 81
PfoI T'CCnGG_A
Cuts at: 2679 2679
Size: 7282
PmeI GTTT'AAAC
Cuts at: 4586 4586
Size: 7282
PmlI CAC'GTG
Cuts at: 2041 2584 2041
Size: 543 6739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5351 5383 6864 6896 5351
Size: 32 1481 32 5737
Fragments arranged by size:
5737 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2061 2101 2794 2032
Size: 29 40 693 6520
Fragments arranged by size:
6520 693 40 29
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7282
PsiI TTA'TAA
Cuts at: 4852 4852
Size: 7282
PspOMI G'GGCC_C
Cuts at: 3081 3117 3443 3081
Size: 36 326 6920
Fragments arranged by size:
6920 326 36
PstI C_TGCA'G
Cuts at: 830 2925 2934 3205 3673 3897 4634 830
Size: 2095 9 271 468 224 737 3478
Fragments arranged by size:
3478 2095 737 468 271 224 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7282
PvuII CAG'CTG
Cuts at: 2626 3712 4078 4410 2626
Size: 1086 366 332 5498
Fragments arranged by size:
5498 1086 366 332
RsrII CG'GwC_CG
Cuts at: 2995 2995
Size: 7282
SacI G_AGCT'C
Cuts at: 2237 2766 2237
Size: 529 6753
SacII CC_GC'GG
Cuts at: 2374 2390 2374
Size: 16 7266
SalI G'TCGA_C
Cuts at: 1936 4624 1936
Size: 2688 4594
SanDI GG'GwC_CC
Cuts at: 2061 2061
Size: 7282
SapI GCTCTTCn'nnn_
Cuts at: 3341 4202 3341
Size: 861 6421
SbfI CC_TGCA'GG
Cuts at: 3673 4634 3673
Size: 961 6321
ScaI AGT'ACT
Cuts at: 5530 7242 5530
Size: 1712 5570
SexAI A'CCwGG_T
Cuts at: 3954 3954
Size: 7282
SfcI C'TryA_G
Cuts at: 826 1045 2921 2930 3201 3669 3893 4126 4630
Size: 219 1876 9 271 468 224 233 504
Cuts at: 4630 5765 6446 6637 826
Size: 1135 681 191 1471
Fragments arranged by size:
1876 1471 1135 681 504 468 271 233
224 219 191 9
SfoI GGC'GCC
Cuts at: 3067 3067
Size: 7282
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
SgrAI Cr'CCGG_yG
Cuts at: 1700 2551 3437 1700
Size: 851 886 5545
Fragments arranged by size:
5545 886 851
SmaI CCC'GGG
Cuts at: 1872 3312 4609 1872
Size: 1440 1297 4545
Fragments arranged by size:
4545 1440 1297
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 2426 2711 2909 3717 5386
Size: 197 926 219 264 285 198 808 1669
Cuts at: 5386 6287 6549 820
Size: 901 262 1553
Fragments arranged by size:
1669 1553 926 901 808 285 264 262
219 198 197
SnaBI TAC'GTA
Cuts at: 493 4579 5073 493
Size: 4086 494 2702
Fragments arranged by size:
4086 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7282
SphI G_CATG'C
Cuts at: 1132 2857 4640 1132
Size: 1725 1783 3774
Fragments arranged by size:
3774 1783 1725
SspI AAT'ATT
Cuts at: 5 52 5206 5
Size: 47 5154 2081
Fragments arranged by size:
5154 2081 47
StuI AGG'CCT
Cuts at: 1517 2972 3139 3273 3667 1517
Size: 1455 167 134 394 5132
Fragments arranged by size:
5132 1455 394 167 134
StyI C'CwwG_G
Cuts at: 513 1065 2022 2097 2883 4461 4721 513
Size: 552 957 75 786 1578 260 3074
Fragments arranged by size:
3074 1578 957 786 552 260 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2461 3383 3395 5309 5326 5479 5664
Size: 1255 881 922 12 1914 17 153 185
Cuts at: 5664 6109 325
Size: 445 1498
Fragments arranged by size:
1914 1498 1255 922 881 445 185 153
17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2704 5528 7240
Size: 274 80 33 51 1443 727 2824 1712
Cuts at: 7240 96
Size: 138
Fragments arranged by size:
2824 1712 1443 727 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2073 2105 3141 3170 3173 3202 7242 7274 2073
Size: 32 1036 29 3 29 4040 32 2081
Fragments arranged by size:
4040 2081 1036 32 32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 1316 4283 1316
Size: 2967 4315
XbaI T'CTAG_A
Cuts at: 4618 4618
Size: 7282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4063 4063
Size: 7282
XhoI C'TCGA_G
Cuts at: 1943 2426 3717 1943
Size: 483 1291 5508
Fragments arranged by size:
5508 1291 483
XmaI C'CCGG_G
Cuts at: 1870 3310 4607 1870
Size: 1440 1297 4545
Fragments arranged by size:
4545 1440 1297
XmnI GAAnn'nnTTC
Cuts at: 5411 5411
Size: 7282
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2268 5090 276
Size: 53 83 186 936 734 2822 2468
Fragments arranged by size:
2822 2468 936 734 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NsiI
PacI PspXI PsrI SfiI SrfI SwaI