Restriction map for
pFN21ASDA1504
(Circular) (Six-base) MAPSORT of: pFN21ASDA1504.seq Check: 942 from: 1 to: 6007
pFN21ASDA1504 6007 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2496 3350 1937
Size: 559 854 4594
Fragments arranged by size:
4594 854 559
Acc65I G'GTAC_C
Cuts at: 2819 3328 2819
Size: 509 5498
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4070 5454 5722 1632
Size: 2438 1384 268 1917
Fragments arranged by size:
2438 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 4906 5883 1203
Size: 3703 977 1327
Fragments arranged by size:
3703 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 4736 4736
Size: 6007
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6007
AlwNI CAG_nnn'CTG
Cuts at: 3750 5322 3750
Size: 1572 4435
ApaLI G'TGCA_C
Cuts at: 4064 5220 4064
Size: 1156 4851
ApoI r'AATT_y
Cuts at: 1493 2128 2269 3247 3268 3316 3542 3694 5803
Size: 635 141 978 21 48 226 152 2109
Cuts at: 5803 1493
Size: 1697
Fragments arranged by size:
2109 1697 978 635 226 152 141 48
21
AseI AT'TA_AT
Cuts at: 160 1032 2558 4561 160
Size: 872 1526 2003 1606
Fragments arranged by size:
2003 1606 1526 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 3332 1870
Size: 73 1389 4545
Fragments arranged by size:
4545 1389 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2819 3328 4783 618
Size: 325 217 627 1032 509 1455 1842
Fragments arranged by size:
1842 1455 1032 627 509 325 217
BanII G_rGCy'C
Cuts at: 1391 2417 3326 1391
Size: 1026 909 4072
Fragments arranged by size:
4072 1026 909
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2180 3073 928
Size: 971 281 893 3862
Fragments arranged by size:
3862 971 893 281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4198 4232 1903
Size: 34 2261 34 3678
Fragments arranged by size:
3678 2261 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3032 6002 3032
Size: 2970 3037
BlpI GC'TnA_GC
Cuts at: 3424 3424
Size: 6007
Bme1580I G_kGCm'C
Cuts at: 1790 4068 5224 1790
Size: 2278 1156 2573
Fragments arranged by size:
2573 2278 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2912 3738 4696
Size: 416 53 83 399 24 1495 826 958
Cuts at: 4696 442
Size: 1753
Fragments arranged by size:
1753 1495 958 826 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2436 2436
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2568 2956 3204 4667 1239
Size: 1329 388 248 1463 2579
Fragments arranged by size:
2579 1463 1329 388 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4132 4997 5295 4132
Size: 865 298 4844
Fragments arranged by size:
4844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4670 882
Size: 617 3171 2219
Fragments arranged by size:
3171 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3304 3798 493
Size: 711 2100 494 2702
Fragments arranged by size:
2702 2100 711 494
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2941 3814 4196 4831
Size: 53 83 186 936 1408 873 382 635
Cuts at: 4831 275
Size: 1451
Fragments arranged by size:
1451 1408 936 873 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5782
Fragments arranged by size:
5782 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2610 1186
Size: 1424 4583
BseYI C'CCAG_C
Cuts at: 2054 5210 5706 2054
Size: 3156 496 2355
Fragments arranged by size:
3156 2355 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5434
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2417 4068 4153 5224 2417
Size: 1651 85 1071 3200
Fragments arranged by size:
3200 1651 1071 85
BsmI GAATG_Cn'
Cuts at: 3518 3611 3518
Size: 93 5914
BsmBI CGTCTCn'nnnn_
Cuts at: 2948 2948
Size: 6007
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2417 3326 4068 4153 5224 1391
Size: 399 627 909 742 85 1071 2174
Fragments arranged by size:
2174 1071 909 742 627 399 85
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 2095 2590 2884 3896 5626 2095
Size: 495 294 1012 1730 2476
Fragments arranged by size:
2476 1730 1012 495 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3362 844
Size: 2518 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3894 1394
Size: 429 2071 3507
Fragments arranged by size:
3507 2071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4651 4838 1700
Size: 110 2841 187 2869
Fragments arranged by size:
2869 2841 187 110
BsrGI T'GTAC_A
Cuts at: 96 2501 2652 96
Size: 2405 151 3451
Fragments arranged by size:
3451 2405 151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 4067 5079 4067
Size: 1012 4995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6007
BstBI TT'CG_AA
Cuts at: 2007 4811 2007
Size: 2804 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstXI CCAn_nnnn'nTGG
Cuts at: 3178 3178
Size: 6007
BstYI r'GATC_y
Cuts at: 1972 2698 3032 3337 3704 4089 4106 5558 5644
Size: 726 334 305 367 385 17 1452 86
Cuts at: 5644 5656 6002 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 726 385 367 346 334 305
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2484 2484
Size: 6007
BtgI C'CryG_G
Cuts at: 513 1065 2984 3070 513
Size: 552 1919 86 3450
Fragments arranged by size:
3450 1919 552 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 3523 4317 4337 3523
Size: 794 20 5193
Fragments arranged by size:
5193 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2641 5014 809
Size: 507 1325 2373 1802
Fragments arranged by size:
2373 1802 1325 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3454 4343 5972
Size: 54 1425 50 275 18 1624 889 1629
Cuts at: 5972 8
Size: 43
Fragments arranged by size:
1629 1624 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2811 2984 3937 1353
Size: 1458 173 953 3423
Fragments arranged by size:
3423 1458 953 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2415 2415
Size: 6007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2568 2956 3204 4070 4667 5454 5722
Size: 393 936 388 248 866 597 787 268
Cuts at: 5722 1239
Size: 1524
Fragments arranged by size:
1524 936 866 787 597 393 388 268
248
EcoO109I rG'GnC_Cy
Cuts at: 2051 2051
Size: 6007
EcoRI G'AATT_C
Cuts at: 2269 3316 3694 2269
Size: 1047 378 4582
Fragments arranged by size:
4582 1047 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2204 2946 2204
Size: 742 5265
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 5154 1678
Size: 3476 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4648 4680 4722 4754 1373
Size: 32 135 32 3076 32 42 32 2626
Fragments arranged by size:
3076 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2971 3351 3597 669
Size: 988 281 1033 380 246 3079
Fragments arranged by size:
3079 1033 988 380 281 246
HindIII A'AGCT_T
Cuts at: 748 2847 748
Size: 2099 3908
HpaI GTT'AAC
Cuts at: 2971 3597 2971
Size: 626 5381
KpnI G_GTAC'C
Cuts at: 2823 3332 2823
Size: 509 5498
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2405 3008 5121 5305 1305
Size: 1100 603 2113 184 2007
Fragments arranged by size:
2113 2007 1100 603 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4185
Fragments arranged by size:
4185 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3237 3965 4324 4483 518
Size: 648 2071 728 359 159 2042
Fragments arranged by size:
2071 2042 728 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3423 4100 5248 5493 1606
Size: 1817 677 1148 245 2120
Fragments arranged by size:
2120 1817 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2984 3070 513
Size: 552 1919 86 3450
Fragments arranged by size:
3450 1919 552 86
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 3365 4910 1132
Size: 97 2136 1545 2229
Fragments arranged by size:
2229 2136 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6007
PfoI T'CCnGG_A
Cuts at: 2933 2933
Size: 6007
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2051 2051
Size: 6007
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 2679 3577 2679
Size: 898 5109
PstI C_TGCA'G
Cuts at: 830 3359 830
Size: 2529 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
SacI G_AGCT'C
Cuts at: 2417 2417
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SanDI GG'GwC_CC
Cuts at: 2051 2051
Size: 6007
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SfcI C'TryA_G
Cuts at: 826 1045 2964 3355 4490 5171 5362 826
Size: 219 1919 391 1135 681 191 1471
Fragments arranged by size:
1919 1471 1135 681 391 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4111 5012 5274 820
Size: 197 926 2168 901 262 1553
Fragments arranged by size:
2168 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3304 3798 493
Size: 2811 494 2702
Fragments arranged by size:
2811 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 3286 1517
Size: 1769 4238
StyI C'CwwG_G
Cuts at: 513 1065 2984 3070 3446 513
Size: 552 1919 86 376 3074
Fragments arranged by size:
3074 1919 552 376 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3079 4034 4051 4204 4389 4834 325
Size: 1255 1499 955 17 153 185 445 1498
Fragments arranged by size:
1499 1498 1255 955 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2501 2652 4253
Size: 274 80 33 51 1443 524 151 1601
Cuts at: 4253 5965 96
Size: 1712 138
Fragments arranged by size:
1712 1601 1443 524 274 151 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5967 5999 5967
Size: 32 5975
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6007
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 2103 2929 4136 2103
Size: 826 1207 3974
Fragments arranged by size:
3974 1207 826
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI