Restriction map for
pFN21ASDA1498
(Circular) (Six-base) MAPSORT of: pFN21ASDA1498.seq Check: 1166 from: 1 to: 13858
pFN21ASDA1498 13858 bp
With 182 enzymes: SgfI *
February 10, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2468 2854 3907 8692 8863 9922 10921 10987 2468
Size: 386 1053 4785 171 1059 999 66 5339
Fragments arranged by size:
5339 4785 1059 1053 999 386 171 66
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2966 5094 7417 11668
Size: 53 83 186 936 1430 2128 2323 4251
Cuts at: 11668 278
Size: 2468
Fragments arranged by size:
4251 2468 2323 2128 1430 936 186 83
53
AccI GT'mk_AC
Cuts at: 1937 8373 11201 1937
Size: 6436 2828 4594
Fragments arranged by size:
6436 4594 2828
Acc65I G'GTAC_C
Cuts at: 11179 11179
Size: 13858
AclI AA'CG_TT
Cuts at: 1563 5395 11985 12358 1563
Size: 3832 6590 373 3063
Fragments arranged by size:
6590 3832 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3179 5127 6125 6234 6308 7376 7428 8294
Size: 1547 1948 998 109 74 1068 52 866
Cuts at: 8294 8714 10595 10601 11921 13305 13573 1632
Size: 420 1881 6 1320 1384 268 1917
Fragments arranged by size:
1948 1917 1881 1547 1384 1320 1068 998
866 420 268 109 74 52 6
AfeI AGC'GCT
Cuts at: 2344 2344
Size: 13858
AflII C'TTAA_G
Cuts at: 820 1017 10724 820
Size: 197 9707 3954
Fragments arranged by size:
9707 3954 197
AflIII A'CryG_T
Cuts at: 1203 9153 12757 13734 1203
Size: 7950 3604 977 1327
Fragments arranged by size:
7950 3604 1327 977
AgeI A'CCGG_T
Cuts at: 12689 12689
Size: 13858
AhdI GACnn_n'nnGTC
Cuts at: 2424 7122 9754 12587 2424
Size: 4698 2632 2833 3695
Fragments arranged by size:
4698 3695 2833 2632
AleI CACnn'nnGTG
Cuts at: 1166 3035 5099 5543 7634 8846 9794 10307 1166
Size: 1869 2064 444 2091 1212 948 513 4717
Fragments arranged by size:
4717 2091 2064 1869 1212 948 513 444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3678 5310 6014 6998 10716 1184
Size: 2494 1632 704 984 3718 4326
Fragments arranged by size:
4326 3718 2494 1632 984 704
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2625 2657 2625
Size: 32 13826
AlwNI CAG_nnn'CTG
Cuts at: 2079 2203 2779 2869 3127 3157 6622 7264 7297
Size: 124 576 90 258 30 3465 642 33
Cuts at: 7297 7537 7858 8081 8821 9533 10147 10570 10573
Size: 240 321 223 740 712 614 423 3
Cuts at: 10573 10768 11601 13173 2079
Size: 195 833 1572 2764
Fragments arranged by size:
3465 2764 1572 833 740 712 642 614
576 423 321 258 240 223 195 124
90 33 30 3
ApaI G_GGCC'C
Cuts at: 2264 5339 5755 6416 10637 10780 2264
Size: 3075 416 661 4221 143 5342
Fragments arranged by size:
5342 4221 3075 661 416 143
ApaLI G'TGCA_C
Cuts at: 5699 6728 9695 10556 11915 13071 5699
Size: 1029 2967 861 1359 1156 6486
Fragments arranged by size:
6486 2967 1359 1156 1029 861
ApoI r'AATT_y
Cuts at: 1493 4627 8652 11167 11393 11545 13654 1493
Size: 3134 4025 2515 226 152 2109 1697
Fragments arranged by size:
4025 3134 2515 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 12412 160
Size: 872 11380 1606
Fragments arranged by size:
11380 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13858
AvaI C'yCGr_G
Cuts at: 1870 1943 2656 2930 3695 4536 5133 6332 6662
Size: 73 713 274 765 841 597 1199 330
Cuts at: 6662 8768 10338 11183 1870
Size: 2106 1570 845 4545
Fragments arranged by size:
4545 2106 1570 1199 845 841 765 713
597 330 274 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6065 6098 7582 7615 6065
Size: 33 1484 33 12308
Fragments arranged by size:
12308 1484 33 33
BamHI G'GATC_C
Cuts at: 11188 11555 11188
Size: 367 13491
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2170 2480 2717 4889 6045
Size: 325 217 627 383 310 237 2172 1156
Cuts at: 6045 6734 7210 8704 9554 9859 10286 10301 10387
Size: 689 476 1494 850 305 427 15 86
Cuts at: 10387 11056 11179 12634 618
Size: 669 123 1455 1842
Fragments arranged by size:
2172 1842 1494 1455 1156 850 689 669
627 476 427 383 325 310 305 237
217 123 86 15
BanII G_rGCy'C
Cuts at: 1391 2219 2264 2573 2616 2638 2747 5185 5339
Size: 828 45 309 43 22 109 2438 154
Cuts at: 5339 5688 5741 5755 6033 6042 6416 6685 8331
Size: 349 53 14 278 9 374 269 1646
Cuts at: 8331 10253 10637 10780 11177 1391
Size: 1922 384 143 397 4072
Fragments arranged by size:
4072 2438 1922 1646 828 397 384 374
349 309 278 269 154 143 109 53
45 43 22 14 9
BbeI G_GCGC'C
Cuts at: 4893 6738 4893
Size: 1845 12013
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3160 3812 8282 928
Size: 971 1261 652 4470 6504
Fragments arranged by size:
6504 4470 1261 971 652
BbvCI CC'TCA_GC
Cuts at: 2155 4099 4277 7340 7908 8560 2155
Size: 1944 178 3063 568 652 7453
Fragments arranged by size:
7453 3063 1944 652 568 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2148 2182 7493 7527 12049 12083 1903
Size: 34 211 34 5311 34 4522 34 3678
Fragments arranged by size:
5311 4522 3678 211 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4213 6093 11750 12960 4213
Size: 1880 5657 1210 5111
Fragments arranged by size:
5657 5111 1880 1210
BclI T'GATC_A
Cuts at: 1548 9262 1548
Size: 7714 6144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3700 4532 9391
Size: 107 122 71 631 605 2028 832 4859
Cuts at: 9391 9506 10406 12469 136
Size: 115 900 2063 1525
Fragments arranged by size:
4859 2063 2028 1525 900 832 631 605
122 115 107 71
BglII A'GATC_T
Cuts at: 6139 7783 9012 13853 6139
Size: 1644 1229 4841 6144
Fragments arranged by size:
6144 4841 1644 1229
BlpI GC'TnA_GC
Cuts at: 2346 4851 6492 6531 8505 8748 11275 2346
Size: 2505 1641 39 1974 243 2527 4929
Fragments arranged by size:
4929 2527 2505 1974 1641 243 39
Bme1580I G_kGCm'C
Cuts at: 1790 2264 2485 2720 3006 5339 5703 5755 5973
Size: 474 221 235 286 2333 364 52 218
Cuts at: 5973 6416 6732 7000 9699 9864 10291 10304 10560
Size: 443 316 268 2699 165 427 13 256
Cuts at: 10560 10637 10780 11061 11919 13075 1790
Size: 77 143 281 858 1156 2573
Fragments arranged by size:
2699 2573 2333 1156 858 474 443 427
364 316 286 281 268 256 235 221
218 165 143 77 52 13
BmgBI CAC'GTC
Cuts at: 1318 2394 3033 5491 5502 1318
Size: 1076 639 2458 11 9674
Fragments arranged by size:
9674 2458 1076 639 11
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2170 2178 2433
Size: 416 53 83 399 24 753 8 255
Cuts at: 2433 4357 5617 5751 8800 9543 9827 10069 10177
Size: 1924 1260 134 3049 743 284 242 108
Cuts at: 10177 10726 11589 12547 442
Size: 549 863 958 1753
Fragments arranged by size:
3049 1924 1753 1260 958 863 753 743
549 416 399 284 255 242 134 108
83 53 24 8
BmtI G_CTAG'C
Cuts at: 1056 4737 8129 1056
Size: 3681 3392 6785
Fragments arranged by size:
6785 3681 3392
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4200 5760 6259 9908 4200
Size: 1560 499 3649 8150
Fragments arranged by size:
8150 3649 1560 499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 2560 2633 3524 4065 4107 4265 4382
Size: 1071 250 73 891 541 42 158 117
Cuts at: 4382 5702 5707 6098 6134 6629 6948 8030 8330
Size: 1320 5 391 36 495 319 1082 300
Cuts at: 8330 8843 10304 11009 11016 12518 1239
Size: 513 1461 705 7 1502 2579
Fragments arranged by size:
2579 1502 1461 1320 1082 1071 891 705
541 513 495 391 319 300 250 158
117 73 42 36 7 5
Bpu10I CC'TnA_GC
Cuts at: 2155 4099 4277 5735 5987 6986 7335 7340 7680
Size: 1944 178 1458 252 999 349 5 340
Cuts at: 7680 7806 7908 8560 2155
Size: 126 102 652 7453
Fragments arranged by size:
7453 1944 1458 999 652 349 340 252
178 126 102 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2587 5357 6942 7273 7976 10803 11983 12848 13146
Size: 2770 1585 331 703 2827 1180 865 298
Cuts at: 13146 2587
Size: 3299
Fragments arranged by size:
3299 2827 2770 1585 1180 865 703 331
298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2376 2680 4657 10456 12521 882
Size: 617 877 304 1977 5799 2065 2219
Fragments arranged by size:
5799 2219 2065 1977 877 617 304
BsaAI yAC'GTr
Cuts at: 493 1204 6064 8524 11155 11649 493
Size: 711 4860 2460 2631 494 2702
Fragments arranged by size:
4860 2702 2631 2460 711 494
BsaBI GATnn'nnATC
Cuts at: 5057 13852 5057
Size: 8795 5063
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2963 4306 4890 5091
Size: 53 83 186 936 1430 1343 584 201
Cuts at: 5091 6542 6735 7414 9635 10112 11012 11665 12047
Size: 1451 193 679 2221 477 900 653 382
Cuts at: 12047 12682 275
Size: 635 1451
Fragments arranged by size:
2221 1451 1451 1430 1343 936 900 679
653 635 584 477 382 201 193 186
83 53
BsaWI w'CCGG_w
Cuts at: 3097 6457 7180 10423 12291 12689 12963 13110 13521
Size: 3360 723 3243 1868 398 274 147 411
Cuts at: 13521 3097
Size: 3434
Fragments arranged by size:
3434 3360 3243 1868 723 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2292 2322 2625 2655 3631
Size: 30 165 30 889 30 303 30 976
Cuts at: 3631 3661 4358 4388 4391 4421 4532 4562 5723
Size: 30 697 30 3 30 111 30 1161
Cuts at: 5723 5753 6071 6101 6465 6495 7297 7327 11125
Size: 30 318 30 364 30 802 30 3798
Cuts at: 11125 11155 1178
Size: 30 3881
Fragments arranged by size:
3881 3798 1161 976 889 802 697 364
318 303 165 111 30 30 30 30
30 30 30 30 30 30 30 30
30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 3092 3320 3323 3326 3329 3338 3727
Size: 928 978 228 3 3 3 9 389
Cuts at: 3727 4379 4417 4475 4802 5845 7558 7925 8027
Size: 652 38 58 327 1043 1713 367 102
Cuts at: 8027 8135 8333 8601 8984 9109 10444 1186
Size: 108 198 268 383 125 1335 4600
Fragments arranged by size:
4600 1713 1335 1043 978 928 652 389
383 367 327 268 228 198 125 108
102 58 38 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2312 2406 2497 2694 2936 3088 3709 3842 5267
Size: 94 91 197 242 152 621 133 1425
Cuts at: 5267 5272 6259 6356 6767 8047 9045 9294 9309
Size: 5 987 97 411 1280 998 249 15
Cuts at: 9309 9417 9435 9457 9598 9702 9730 10081 10254
Size: 108 18 22 141 104 28 351 173
Cuts at: 10254 10392 10548 10779 10875 11047 13061 13557 2312
Size: 138 156 231 96 172 2014 496 2613
Fragments arranged by size:
2613 2014 1425 1280 998 987 621 496
411 351 249 242 231 197 173 172
156 152 141 138 133 108 104 97
96 94 91 28 22 18 15 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2496 3674 3819 3861 3935 3941 5306
Size: 573 760 1178 145 42 74 6 1365
Cuts at: 5306 5651 6335 6759 7370 9246 9470 9516 9857
Size: 345 684 424 611 1876 224 46 341
Cuts at: 9857 9900 10241 10611 10808 10955 1163
Size: 43 341 370 197 147 4066
Fragments arranged by size:
4066 1876 1365 1178 760 684 611 573
424 370 345 341 341 224 197 147
145 74 46 43 42 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3558 4922 6431 6759 7220 8604
Size: 275 186 1557 1364 1509 328 461 1384
Cuts at: 8604 9288 9810 10509 10923 11308 12069 13097 1540
Size: 684 522 699 414 385 761 1028 2301
Fragments arranged by size:
2301 1557 1509 1384 1364 1028 761 699
684 522 461 414 385 328 275 186
BsiHKAI G_wGCw'C
Cuts at: 5134 5688 5703 5741 6732 7275 8331 9699 10560
Size: 554 15 38 991 543 1056 1368 861
Cuts at: 10560 11919 12004 13075 5134
Size: 1359 85 1071 5917
Fragments arranged by size:
5917 1368 1359 1071 1056 991 861 554
543 85 38 15
BsiWI C'GTAC_G
Cuts at: 9002 9002
Size: 13858
BsmI GAATG_Cn'
Cuts at: 3576 4195 6826 9037 11369 11462 3576
Size: 619 2631 2211 2332 93 5972
Fragments arranged by size:
5972 2631 2332 2211 619 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3401 6533 3401
Size: 3132 10726
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2264 2485 2573 2616 2638 2720
Size: 399 429 45 221 88 43 22 82
Cuts at: 2720 2747 3006 5134 5185 5339 5688 5703 5741
Size: 27 259 2128 51 154 349 15 38
Cuts at: 5741 5755 5973 6033 6042 6416 6685 6732 7000
Size: 14 218 60 9 374 269 47 268
Cuts at: 7000 7275 8331 9699 9864 10253 10291 10304 10560
Size: 275 1056 1368 165 389 38 13 256
Cuts at: 10560 10637 10780 11061 11177 11919 12004 13075 1391
Size: 77 143 281 116 742 85 1071 2174
Fragments arranged by size:
2174 2128 1368 1071 1056 742 429 399
389 374 349 281 275 269 268 259
256 221 218 165 154 143 116 88
85 82 77 60 51 47 45 43
38 38 27 22 15 14 13 9
BspEI T'CCGG_A
Cuts at: 3097 6457 13521 3097
Size: 3360 7064 3434
Fragments arranged by size:
7064 3434 3360
BspHI T'CATG_A
Cuts at: 11747 13477 11747
Size: 1730 12128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2401 2468 2854 3907 4277 6288 8692 8863
Size: 1557 67 386 1053 370 2011 2404 171
Cuts at: 8863 9922 10921 10987 11213 844
Size: 1059 999 66 226 3489
Fragments arranged by size:
3489 2404 2011 1557 1059 1053 999 386
370 226 171 67 66
BsrBI CCG'CTC
Cuts at: 1394 1823 2308 2683 5967 6946 8410 8536 8919
Size: 429 485 375 3284 979 1464 126 383
Cuts at: 8919 9785 11023 11745 1394
Size: 866 1238 722 3507
Fragments arranged by size:
3507 3284 1464 1238 979 866 722 485
429 383 375 126
BsrDI GCAATG_nn'
Cuts at: 65 1248 4266 5082 5292 7953 12353 12527 65
Size: 1183 3018 816 210 2661 4400 174 1396
Fragments arranged by size:
4400 3018 2661 1396 1183 816 210 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 2765 3713 6544 6758 9283 10717
Size: 110 297 658 948 2831 214 2525 1434
Cuts at: 10717 12502 12689 1700
Size: 1785 187 2869
Fragments arranged by size:
2869 2831 2525 1785 1434 948 658 297
214 187 110
BsrGI T'GTAC_A
Cuts at: 96 7230 9051 96
Size: 7134 1821 4903
Fragments arranged by size:
7134 4903 1821
BssHII G'CGCG_C
Cuts at: 1925 10686 1925
Size: 8761 5097
BssSI C'ACGA_G
Cuts at: 4457 5541 6188 8964 9055 11918 12930 4457
Size: 1084 647 2776 91 2863 1012 5385
Fragments arranged by size:
5385 2863 2776 1084 1012 647 91
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2062 2461 2470 2866 2869 3436 3550 8704
Size: 1268 399 9 396 3 567 114 5154
Cuts at: 8704 10147 10570 10933 794
Size: 1443 423 363 3719
Fragments arranged by size:
5154 3719 1443 1268 567 423 399 396
363 114 9 3
BstBI TT'CG_AA
Cuts at: 2007 12662 2007
Size: 10655 3203
BstEII G'GTnAC_C
Cuts at: 1184 3488 11002 1184
Size: 2304 7514 4040
Fragments arranged by size:
7514 4040 2304
BstXI CCAn_nnnn'nTGG
Cuts at: 2036 2548 3451 9211 2036
Size: 512 903 5760 6683
Fragments arranged by size:
6683 5760 903 512
BstYI r'GATC_y
Cuts at: 1972 5718 6139 7248 7783 8875 9012 10059 10797
Size: 3746 421 1109 535 1092 137 1047 738
Cuts at: 10797 11188 11555 11940 11957 13409 13495 13507 13853
Size: 391 367 385 17 1452 86 12 346
Cuts at: 13853 1972
Size: 1977
Fragments arranged by size:
3746 1977 1452 1109 1092 1047 738 535
421 391 385 367 346 137 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2029 2754 2802 3639 3998 4530 5694
Size: 552 964 725 48 837 359 532 1164
Cuts at: 5694 6221 6345 6390 7009 7214 7308 7824 7878
Size: 527 124 45 619 205 94 516 54
Cuts at: 7878 9288 9713 9735 9759 9810 10308 10539 10842
Size: 1410 425 22 24 51 498 231 303
Cuts at: 10842 11007 513
Size: 165 3364
Fragments arranged by size:
3364 1410 1164 964 837 725 619 552
532 527 516 498 425 359 303 231
205 165 124 94 54 51 48 45
24 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3449 6421 9571 487
Size: 683 56 2223 2972 3150 4774
Fragments arranged by size:
4774 3150 2972 2223 683 56
BtsI GCAGTG_nn'
Cuts at: 2300 3128 3680 3723 4379 5094 5134 6551 7525
Size: 828 552 43 656 715 40 1417 974
Cuts at: 7525 8241 8253 9499 10956 11374 12168 12188 2300
Size: 716 12 1246 1457 418 794 20 3970
Fragments arranged by size:
3970 1457 1417 1246 974 828 794 716
715 656 552 418 43 40 20 12
ClaI AT'CG_AT
Cuts at: 1554 7503 1554
Size: 5949 7909
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4372 4407 5297 5332 529
Size: 35 3808 35 890 35 9055
Fragments arranged by size:
9055 3808 890 35 35 35
DraI TTT'AAA
Cuts at: 8172 11162 8172
Size: 2990 10868
DraIII CAC_nnn'GTG
Cuts at: 2593 5809 2593
Size: 3216 10642
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 11011 12865 809
Size: 507 9695 1854 1802
Fragments arranged by size:
9695 1854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2781 4380 4919
Size: 54 1425 50 275 18 951 1599 539
Cuts at: 4919 5220 6322 6428 6756 7042 7217 9285 9807
Size: 301 1102 106 328 286 175 2068 522
Cuts at: 9807 10311 10770 10845 10920 11305 12194 13823 8
Size: 504 459 75 75 385 889 1629 43
Fragments arranged by size:
2068 1629 1599 1425 1102 951 889 539
522 504 459 385 328 301 286 275
175 106 75 75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4919 6428 6756 7217 9285 9807 10920
Size: 275 3107 1509 328 461 2068 522 1113
Cuts at: 10920 11305 1537
Size: 385 4090
Fragments arranged by size:
4090 3107 2068 1509 1113 522 461 385
328 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3297 3315 4021 4491 4764 4779 5757 5957
Size: 1944 18 706 470 273 15 978 200
Cuts at: 5957 6061 7749 8926 8972 11788 1353
Size: 104 1688 1177 46 2816 3423
Fragments arranged by size:
3423 2816 1944 1688 1177 978 706 470
273 200 104 46 18 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3536 4531 6646 7579 7640 8339
Size: 1064 138 1947 995 2115 933 61 699
Cuts at: 8339 12445 12819 12965 387
Size: 4106 374 146 1280
Fragments arranged by size:
4106 2115 1947 1280 1064 995 933 699
374 146 138 61
EcoICRI GAG'CTC
Cuts at: 5686 5739 8329 5686
Size: 53 2590 11215
Fragments arranged by size:
11215 2590 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2560 2633 3179 3524 4065 4107
Size: 393 678 250 73 546 345 541 42
Cuts at: 4107 4265 4382 5127 5702 5707 6098 6125 6134
Size: 158 117 745 575 5 391 27 9
Cuts at: 6134 6234 6308 6629 6948 7376 7428 8030 8294
Size: 100 74 321 319 428 52 602 264
Cuts at: 8294 8330 8714 8843 10304 10595 10601 11009 11016
Size: 36 384 129 1461 291 6 408 7
Cuts at: 11016 11921 12518 13305 13573 1239
Size: 905 597 787 268 1524
Fragments arranged by size:
1524 1461 905 787 745 678 602 597
575 546 541 428 408 393 391 384
345 321 319 291 268 264 250 158
129 117 100 74 73 52 42 36
27 9 7 6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 5120 7619 5120
Size: 2499 11359
EcoO109I rG'GnC_Cy
Cuts at: 2076 2261 2735 3644 4058 4327 5335 5336 5524
Size: 185 474 909 414 269 1008 1 188
Cuts at: 5524 5727 5751 5752 6412 7032 7261 7987 9454
Size: 203 24 1 660 620 229 726 1467
Cuts at: 9454 9595 9654 10633 10776 11107 2076
Size: 141 59 979 143 331 4827
Fragments arranged by size:
4827 1467 1008 979 909 726 660 620
474 414 331 269 229 203 188 185
143 141 59 24 1 1
EcoRI G'AATT_C
Cuts at: 11167 11545 11167
Size: 378 13480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3184 3290 4639 6158 3184
Size: 106 1349 1519 10884
Fragments arranged by size:
10884 1519 1349 106
FseI GG_CCGG'CC
Cuts at: 2769 2769
Size: 13858
FspI TGC'GCA
Cuts at: 12364 12364
Size: 13858
HaeII r_GCGC'y
Cuts at: 1678 2346 4893 6726 6738 6919 6952 8798 9631
Size: 668 2547 1833 12 181 33 1846 833
Cuts at: 9631 10904 11040 13005 1678
Size: 1273 136 1965 2531
Fragments arranged by size:
2547 2531 1965 1846 1833 1273 833 668
181 136 33 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2386 2410 2442 3139
Size: 32 135 32 782 32 24 32 697
Cuts at: 3139 3171 3601 3633 5400 5432 6071 6103 6188
Size: 32 430 32 1767 32 639 32 85
Cuts at: 6188 6220 6697 6729 7108 7140 7297 7329 8054
Size: 32 477 32 379 32 157 32 725
Cuts at: 8054 8086 9740 9772 10024 10056 12499 12531 12573
Size: 32 1654 32 252 32 2443 32 42
Cuts at: 12573 12605 1373
Size: 32 2626
Fragments arranged by size:
2626 2443 1767 1654 782 725 697 639
477 430 379 252 157 135 85 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 5980 6940 7116 8896 11202 11448
Size: 988 281 4042 960 176 1780 2306 246
Cuts at: 11448 669
Size: 3079
Fragments arranged by size:
4042 3079 2306 1780 988 960 281 246
176
HindIII A'AGCT_T
Cuts at: 748 7374 748
Size: 6626 7232
HpaI GTT'AAC
Cuts at: 8896 11448 8896
Size: 2552 11306
KasI G'GCGC_C
Cuts at: 4889 6734 4889
Size: 1845 12013
KpnI G_GTAC'C
Cuts at: 11183 11183
Size: 13858
MfeI C'AATT_G
Cuts at: 4779 8154 11457 4779
Size: 3375 3303 7180
Fragments arranged by size:
7180 3375 3303
MluI A'CGCG_T
Cuts at: 9153 13734 9153
Size: 4581 9277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7350 7431 11094 12972 13156 1305
Size: 6045 81 3663 1878 184 2007
Fragments arranged by size:
6045 3663 2007 1878 184 81
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2783 4382 5222 6324 10313
Size: 54 1425 343 951 1599 840 1102 3989
Cuts at: 10313 10847 10
Size: 534 3021
Fragments arranged by size:
3989 3021 1599 1425 1102 951 840 534
343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3035 3050 3449 5099 5543 5996 7634
Size: 648 1869 15 399 1650 444 453 1638
Cuts at: 7634 8061 8846 9209 9794 10130 10307 11816 12175
Size: 427 785 363 585 336 177 1509 359
Cuts at: 12175 12334 518
Size: 159 2042
Fragments arranged by size:
2042 1869 1650 1638 1509 785 648 585
453 444 427 399 363 359 336 177
159 15
MspA1I CmG'CkG
Cuts at: 1606 2062 2203 2756 2848 2884 2948 3691 3883
Size: 456 141 553 92 36 64 743 192
Cuts at: 3883 5665 6223 6496 6611 6622 6628 7216 7297
Size: 1782 558 273 115 11 6 588 81
Cuts at: 7297 8564 8747 9049 9196 9352 9371 9401 9602
Size: 1267 183 302 147 156 19 30 201
Cuts at: 9602 10357 10445 10570 11274 11951 13099 13344 1606
Size: 755 88 125 704 677 1148 245 2120
Fragments arranged by size:
2120 1782 1267 1148 755 743 704 677
588 558 553 456 302 273 245 201
192 183 156 147 141 125 115 92
88 81 64 36 30 19 11 6
NaeI GCC'GGC
Cuts at: 2109 2767 6760 9285 10719 2109
Size: 658 3993 2525 1434 5248
Fragments arranged by size:
5248 3993 2525 1434 658
NarI GG'CG_CC
Cuts at: 4890 6735 4890
Size: 1845 12013
NcoI C'CATG_G
Cuts at: 513 1065 2029 5694 7009 7824 7878 9759 513
Size: 552 964 3665 1315 815 54 1881 4612
Fragments arranged by size:
4612 3665 1881 1315 964 815 552 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 13858
NgoMIV G'CCGG_C
Cuts at: 2107 2765 6758 9283 10717 2107
Size: 658 3993 2525 1434 5248
Fragments arranged by size:
5248 3993 2525 1434 658
NheI G'CTAG_C
Cuts at: 1052 4733 8125 1052
Size: 3681 3392 6785
Fragments arranged by size:
6785 3681 3392
NotI GC'GGCC_GC
Cuts at: 7217 11305 7217
Size: 4088 9770
NspI r_CATG'y
Cuts at: 1132 1229 5882 8092 9547 10564 11216 12761 1132
Size: 97 4653 2210 1455 1017 652 1545 2229
Fragments arranged by size:
4653 2229 2210 1545 1455 1017 652 97
PasI CC'CwG_GG
Cuts at: 1348 2492 2629 2896 3515 4142 5714 6026 6739
Size: 1144 137 267 619 627 1572 312 713
Cuts at: 6739 1348
Size: 8467
Fragments arranged by size:
8467 1572 1144 713 627 619 312 267
137
PciI A'CATG_T
Cuts at: 12757 12757
Size: 13858
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2731 5788 6794 6971 10283 10768 1108
Size: 1623 3057 1006 177 3312 485 4198
Fragments arranged by size:
4198 3312 3057 1623 1006 485 177
PfoI T'CCnGG_A
Cuts at: 2990 4133 4643 4863 5404 5679 7851 2990
Size: 1143 510 220 541 275 2172 8997
Fragments arranged by size:
8997 2172 1143 541 510 275 220
PmeI GTTT'AAAC
Cuts at: 11162 11162
Size: 13858
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2625 2657 11927 11959 13440 13472 2625
Size: 32 9270 32 1481 32 3011
Fragments arranged by size:
9270 3011 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2735 4058 7032 9595 11107 2735
Size: 1323 2974 2563 1512 5486
Fragments arranged by size:
5486 2974 2563 1512 1323
PshAI GACnn'nnGTC
Cuts at: 1613 8399 1613
Size: 6786 7072
PsiI TTA'TAA
Cuts at: 11428 11428
Size: 13858
PspOMI G'GGCC_C
Cuts at: 2260 5335 5751 6412 10633 10776 2260
Size: 3075 416 661 4221 143 5342
Fragments arranged by size:
5342 4221 3075 661 416 143
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7466 7498 7466
Size: 32 13826
PstI C_TGCA'G
Cuts at: 830 2067 2253 2510 2752 2775 2874 3730 3750
Size: 1237 186 257 242 23 99 856 20
Cuts at: 3750 4323 5631 6009 6558 6627 7146 7329 8248
Size: 573 1308 378 549 69 519 183 919
Cuts at: 8248 8286 8569 8805 10152 10362 10951 11210 830
Size: 38 283 236 1347 210 589 259 3478
Fragments arranged by size:
3478 1347 1308 1237 919 856 589 573
549 519 378 283 259 257 242 236
210 186 183 99 69 38 23 20
PvuI CG_AT'CG
Cuts at: 2001 10509 2001
Size: 8508 5350
PvuII CAG'CTG
Cuts at: 2062 2203 2848 3691 3883 6496 6628 7297 8564
Size: 141 645 843 192 2613 132 669 1267
Cuts at: 8564 8747 9049 9401 9602 10357 10445 10570 2062
Size: 183 302 352 201 755 88 125 5350
Fragments arranged by size:
5350 2613 1267 843 755 669 645 352
302 201 192 183 141 132 125 88
SacI G_AGCT'C
Cuts at: 5688 5741 8331 5688
Size: 53 2590 11215
Fragments arranged by size:
11215 2590 53
SacII CC_GC'GG
Cuts at: 2757 6224 7217 2757
Size: 3467 993 9398
Fragments arranged by size:
9398 3467 993
SalI G'TCGA_C
Cuts at: 1936 11200 1936
Size: 9264 4594
SanDI GG'GwC_CC
Cuts at: 2735 7032 9595 11107 2735
Size: 4297 2563 1512 5486
Fragments arranged by size:
5486 4297 2563 1512
SapI GCTCTTCn'nnn_
Cuts at: 4491 5957 6061 8926 4491
Size: 1466 104 2865 9423
Fragments arranged by size:
9423 2865 1466 104
SbfI CC_TGCA'GG
Cuts at: 8286 11210 8286
Size: 2924 10934
ScaI AGT'ACT
Cuts at: 12106 13818 12106
Size: 1712 12146
SexAI A'CCwGG_T
Cuts at: 4251 9750 4251
Size: 5499 8359
SfcI C'TryA_G
Cuts at: 826 1045 2063 2249 2506 2520 2748 2771 2870
Size: 219 1018 186 257 14 228 23 99
Cuts at: 2870 3726 3746 4319 5627 6005 6554 6623 6831
Size: 856 20 573 1308 378 549 69 208
Cuts at: 6831 7142 7325 8244 8282 8356 8565 8801 8902
Size: 311 183 919 38 74 209 236 101
Cuts at: 8902 10148 10358 10947 11206 12341 13022 13213 826
Size: 1246 210 589 259 1135 681 191 1471
Fragments arranged by size:
1471 1308 1246 1135 1018 919 856 681
589 573 549 378 311 259 257 236
228 219 210 209 208 191 186 183
101 99 74 69 38 23 20 14
SfoI GGC'GCC
Cuts at: 4891 6736 4891
Size: 1845 12013
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13858
SgrAI Cr'CCGG_yG
Cuts at: 1700 6544 1700
Size: 4844 9014
SmaI CCC'GGG
Cuts at: 1872 2658 2932 3697 4538 6664 10340 11185 1872
Size: 786 274 765 841 2126 3676 845 4545
Fragments arranged by size:
4545 3676 2126 845 841 786 765 274
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2566 5372 6957 7252 7991 10724
Size: 197 926 623 2806 1585 295 739 2733
Cuts at: 10724 10818 11962 12863 13125 820
Size: 94 1144 901 262 1553
Fragments arranged by size:
2806 2733 1585 1553 1144 926 901 739
623 295 262 197 94
SnaBI TAC'GTA
Cuts at: 493 11155 11649 493
Size: 10662 494 2702
Fragments arranged by size:
10662 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 13858
SphI G_CATG'C
Cuts at: 1132 9547 11216 1132
Size: 8415 1669 3774
Fragments arranged by size:
8415 3774 1669
SspI AAT'ATT
Cuts at: 5 52 4564 8686 11782 5
Size: 47 4512 4122 3096 2081
Fragments arranged by size:
4512 4122 3096 2081 47
StuI AGG'CCT
Cuts at: 1517 2459 3012 3472 3926 4622 5957 10282 1517
Size: 942 553 460 454 696 1335 4325 5093
Fragments arranged by size:
5093 4325 1335 942 696 553 460 454
StyI C'CwwG_G
Cuts at: 513 1065 2029 3493 4842 5076 5694 6825 7009
Size: 552 964 1464 1349 234 618 1131 184
Cuts at: 7009 7824 7878 9759 10277 10410 11297 513
Size: 815 54 1881 518 133 887 3074
Fragments arranged by size:
3074 1881 1464 1349 1131 964 887 815
618 552 518 234 184 133 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2633 3528 5137 5612 6063 7975 8437
Size: 1255 1053 895 1609 475 451 1912 462
Cuts at: 8437 8452 8604 9059 9716 10499 10762 11885 11902
Size: 15 152 455 657 783 263 1123 17
Cuts at: 11902 12055 12240 12685 325
Size: 153 185 445 1498
Fragments arranged by size:
1912 1609 1498 1255 1123 1053 895 783
657 475 462 455 451 445 263 185
153 152 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7230 9051 12104
Size: 274 80 33 51 1443 5253 1821 3053
Cuts at: 12104 13816 96
Size: 1712 138
Fragments arranged by size:
5253 3053 1821 1712 1443 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2292 2324 2577 2609 3631 3663 4358 4390 4391
Size: 32 253 32 1022 32 695 32 1
Cuts at: 4391 4423 4532 4564 6465 6497 6543 6575 7404
Size: 32 109 32 1901 32 46 32 829
Cuts at: 7404 7436 7558 7590 9387 9419 10327 10359 10524
Size: 32 122 32 1797 32 908 32 165
Cuts at: 10524 10556 13818 13850 2292
Size: 32 3262 32 2300
Fragments arranged by size:
3262 2300 1901 1797 1022 908 829 695
253 165 122 109 46 32 32 32
32 32 32 32 32 32 32 32
32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 1316 6709 7631 8462 1316
Size: 5393 922 831 6712
Fragments arranged by size:
6712 5393 922 831
XbaI T'CTAG_A
Cuts at: 11194 11194
Size: 13858
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2728 10321 2728
Size: 7593 6265
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 13858
XmaI C'CCGG_G
Cuts at: 1870 2656 2930 3695 4536 6662 10338 11183 1870
Size: 786 274 765 841 2126 3676 845 4545
Fragments arranged by size:
4545 3676 2126 845 841 786 765 274
XmnI GAAnn'nnTTC
Cuts at: 11987 11987
Size: 13858
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2964 5092 7415 11666
Size: 53 83 186 936 1430 2128 2323 4251
Cuts at: 11666 276
Size: 2468
Fragments arranged by size:
4251 2468 2323 2128 1430 936 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BstZ17I Bsu36I EcoRV FspAI NruI
NsiI PacI PmlI PspXI RsrII SfiI SrfI SwaI