Restriction map for
pFN21ASDA1486
(Circular) (Six-base) MAPSORT of: pFN21ASDA1486.seq Check: 8563 from: 1 to: 6688
pFN21ASDA1486 6688 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2684 3893 4498 278
Size: 53 83 186 936 1148 1209 605 2468
Fragments arranged by size:
2468 1209 1148 936 605 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2823 3275 3796 3887 4031 1937
Size: 886 452 521 91 144 4594
Fragments arranged by size:
4594 886 521 452 144 91
Acc65I G'GTAC_C
Cuts at: 3955 4009 3955
Size: 54 6634
AclI AA'CG_TT
Cuts at: 1563 4815 5188 1563
Size: 3252 373 3063
Fragments arranged by size:
3252 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 2773 3417 4751 6135 6403 1632
Size: 930 211 644 1334 1384 268 1917
Fragments arranged by size:
1917 1384 1334 930 644 268 211
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6491
AflIII A'CryG_T
Cuts at: 1203 3126 5587 6564 1203
Size: 1923 2461 977 1327
Fragments arranged by size:
2461 1923 1327 977
AgeI A'CCGG_T
Cuts at: 5519 5519
Size: 6688
AhdI GACnn_n'nnGTC
Cuts at: 5417 5417
Size: 6688
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3611 3956 1184
Size: 2427 345 3916
Fragments arranged by size:
3916 2427 345
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3280 3312 3645 3677 3280
Size: 32 333 32 6291
Fragments arranged by size:
6291 333 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2383 2418 4431 6003 2383
Size: 35 2013 1572 3068
Fragments arranged by size:
3068 2013 1572 35
ApaI G_GGCC'C
Cuts at: 2673 2673
Size: 6688
ApaLI G'TGCA_C
Cuts at: 4745 5901 4745
Size: 1156 5532
ApoI r'AATT_y
Cuts at: 1493 3997 4223 4375 6484 1493
Size: 2504 226 152 2109 1697
Fragments arranged by size:
2504 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5242 160
Size: 872 4210 1606
Fragments arranged by size:
4210 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 2735 3517 4013 1870
Size: 73 208 584 782 496 4545
Fragments arranged by size:
4545 782 584 496 208 73
BamHI G'GATC_C
Cuts at: 4018 4385 4018
Size: 367 6321
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2923 3955 4009 5464 618
Size: 325 217 627 1136 1032 54 1455 1842
Fragments arranged by size:
1842 1455 1136 1032 627 325 217 54
BanII G_rGCy'C
Cuts at: 1391 2673 2751 3428 3551 3821 4007 1391
Size: 1282 78 677 123 270 186 4072
Fragments arranged by size:
4072 1282 677 270 186 123 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2067 2227 3720 928
Size: 971 168 160 1493 3896
Fragments arranged by size:
3896 1493 971 168 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2623 2657 4879 4913 1903
Size: 34 686 34 2222 34 3678
Fragments arranged by size:
3678 2222 686 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4580 5790 4580
Size: 1210 5478
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6688
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5299 136
Size: 107 122 71 631 605 3627 1525
Fragments arranged by size:
3627 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6683 6683
Size: 6688
BlpI GC'TnA_GC
Cuts at: 2474 2630 4105 2474
Size: 156 1475 5057
Fragments arranged by size:
5057 1475 156
Bme1580I G_kGCm'C
Cuts at: 1790 2402 2673 4749 5905 1790
Size: 612 271 2076 1156 2573
Fragments arranged by size:
2573 2076 1156 612 271
BmgBI CAC'GTC
Cuts at: 1318 3181 1318
Size: 1863 4825
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3286 4419 5377
Size: 416 53 83 399 24 1869 1133 958
Cuts at: 5377 442
Size: 1753
Fragments arranged by size:
1869 1753 1133 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2338 3116 3221 3575 3718 5348 1239
Size: 827 272 778 105 354 143 1630 2579
Fragments arranged by size:
2579 1630 827 778 354 272 143 105
Bpu10I CC'TnA_GC
Cuts at: 3368 3753 3368
Size: 385 6303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2188 3403 4813 5678 5976 2188
Size: 1215 1410 865 298 2900
Fragments arranged by size:
2900 1410 1215 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2724 5351 882
Size: 617 1225 2627 2219
Fragments arranged by size:
2627 2219 1225 617
BsaAI yAC'GTr
Cuts at: 493 1204 2284 3985 4479 493
Size: 711 1080 1701 494 2702
Fragments arranged by size:
2702 1701 1080 711 494
BsaBI GATnn'nnATC
Cuts at: 6682 6682
Size: 6688
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2681 2868 3155 3725
Size: 53 83 186 936 1148 187 287 570
Cuts at: 3725 3890 4495 4877 5512 275
Size: 165 605 382 635 1451
Fragments arranged by size:
1451 1148 936 635 605 570 382 287
187 186 165 83 53
BsaWI w'CCGG_w
Cuts at: 5121 5519 5793 5940 6351 5121
Size: 398 274 147 411 5458
Fragments arranged by size:
5458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2738 2768 3280 3310 1178
Size: 30 165 30 1335 30 512 30 4556
Fragments arranged by size:
4556 1335 512 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2316 2586 2689 2759 3244 3568 3572 3894
Size: 1130 270 103 70 485 324 4 322
Cuts at: 3894 1186
Size: 3980
Fragments arranged by size:
3980 1130 485 324 322 270 103 70
4
BseYI C'CCAG_C
Cuts at: 2462 5891 6387 2462
Size: 3429 496 2763
Fragments arranged by size:
3429 2763 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 1163
Size: 573 633 5482
Fragments arranged by size:
5482 633 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3586 4138 4899 5927 1540
Size: 275 186 1585 552 761 1028 2301
Fragments arranged by size:
2301 1585 1028 761 552 275 186
BsiHKAI G_wGCw'C
Cuts at: 2480 2636 3428 4749 4834 5905 2480
Size: 156 792 1321 85 1071 3263
Fragments arranged by size:
3263 1321 1071 792 156 85
BsmI GAATG_Cn'
Cuts at: 2140 3403 4199 4292 2140
Size: 1263 796 93 4536
Fragments arranged by size:
4536 1263 796 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2402 2480 2636 2673 2751 3428 3551
Size: 399 612 78 156 37 78 677 123
Cuts at: 3551 3821 4007 4749 4834 5905 1391
Size: 270 186 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 677 612 399 270 186
156 123 85 78 78 37
BspEI T'CCGG_A
Cuts at: 6351 6351
Size: 6688
BspHI T'CATG_A
Cuts at: 2310 4577 6307 2310
Size: 2267 1730 2691
Fragments arranged by size:
2691 2267 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3015 3238 3948 4043 844
Size: 2171 223 710 95 3489
Fragments arranged by size:
3489 2171 710 223 95
BsrBI CCG'CTC
Cuts at: 1394 1823 2520 4575 1394
Size: 429 697 2055 3507
Fragments arranged by size:
3507 2055 697 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3055 5183 5357 65
Size: 1183 1807 2128 174 1396
Fragments arranged by size:
2128 1807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3615 3876 5332 5519 1700
Size: 110 1805 261 1456 187 2869
Fragments arranged by size:
2869 1805 1456 261 187 110
BsrGI T'GTAC_A
Cuts at: 96 2754 96
Size: 2658 4030
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6688
BssSI C'ACGA_G
Cuts at: 3544 4748 5760 3544
Size: 1204 1012 4472
Fragments arranged by size:
4472 1204 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6688
BstBI TT'CG_AA
Cuts at: 2007 5492 2007
Size: 3485 3203
BstEII G'GTnAC_C
Cuts at: 1184 3787 1184
Size: 2603 4085
BstYI r'GATC_y
Cuts at: 1972 4018 4385 4770 4787 6239 6325 6337 6683
Size: 2046 367 385 17 1452 86 12 346
Cuts at: 6683 1972
Size: 1977
Fragments arranged by size:
2046 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2055 2328 3923 2055
Size: 273 1595 4820
Fragments arranged by size:
4820 1595 273
BtgI C'CryG_G
Cuts at: 513 1065 2690 2724 2919 3216 3301 3492 3718
Size: 552 1625 34 195 297 85 191 226
Cuts at: 3718 513
Size: 3483
Fragments arranged by size:
3483 1625 552 297 226 195 191 85
34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2586 487
Size: 683 56 1360 4589
Fragments arranged by size:
4589 1360 683 56
BtsI GCAGTG_nn'
Cuts at: 2502 2912 3940 4204 4998 5018 2502
Size: 410 1028 264 794 20 4172
Fragments arranged by size:
4172 1028 794 410 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6688
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6653
DraI TTT'AAA
Cuts at: 3992 3992
Size: 6688
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2545 5695 809
Size: 507 1229 3150 1802
Fragments arranged by size:
3150 1802 1229 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3187 3583 3613
Size: 54 1425 50 275 18 1357 396 30
Cuts at: 3613 4135 5024 6653 8
Size: 522 889 1629 43
Fragments arranged by size:
1629 1425 1357 889 522 396 275 54
50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3583 4135 1537
Size: 275 1771 552 4090
Fragments arranged by size:
4090 1771 552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2736 3578 4618 1353
Size: 1383 842 1040 3423
Fragments arranged by size:
3423 1383 1040 842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2982 5275 5649 5795 387
Size: 1064 138 1393 2293 374 146 1280
Fragments arranged by size:
2293 1393 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3426 3426
Size: 6688
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2338 2562 2773 3116 3221 3417
Size: 393 434 272 224 211 343 105 196
Cuts at: 3417 3575 3718 4751 5348 6135 6403 1239
Size: 158 143 1033 597 787 268 1524
Fragments arranged by size:
1524 1033 787 597 434 393 343 272
268 224 211 196 158 143 105
EcoO109I rG'GnC_Cy
Cuts at: 2458 2670 3227 3354 2458
Size: 212 557 127 5792
Fragments arranged by size:
5792 557 212 127
EcoRI G'AATT_C
Cuts at: 3997 4375 3997
Size: 378 6310
EcoRV GAT'ATC
Cuts at: 2028 2028
Size: 6688
FspI TGC'GCA
Cuts at: 3599 5194 3599
Size: 1595 5093
HaeII r_GCGC'y
Cuts at: 1678 3622 5835 1678
Size: 1944 2213 2531
Fragments arranged by size:
2531 2213 1944
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2035 2067 2581 2613 3729
Size: 32 135 32 463 32 514 32 1116
Cuts at: 3729 3761 5329 5361 5403 5435 1373
Size: 32 1568 32 42 32 2626
Fragments arranged by size:
2626 1568 1116 514 463 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3487 3797 3888 4032 4278 669
Size: 988 281 1549 310 91 144 246 3079
Fragments arranged by size:
3079 1549 988 310 281 246 144 91
HindIII A'AGCT_T
Cuts at: 748 3107 748
Size: 2359 4329
HpaI GTT'AAC
Cuts at: 4278 4278
Size: 6688
KpnI G_GTAC'C
Cuts at: 3959 4013 3959
Size: 54 6634
MfeI C'AATT_G
Cuts at: 4287 4287
Size: 6688
MluI A'CGCG_T
Cuts at: 6564 6564
Size: 6688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3096 3675 5802 5986 1305
Size: 1791 579 2127 184 2007
Fragments arranged by size:
2127 2007 1791 579 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4866
Fragments arranged by size:
4866 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2571 2762 4646 5005 5164 518
Size: 648 1149 256 191 1884 359 159 2042
Fragments arranged by size:
2042 1884 1149 648 359 256 191 159
MspA1I CmG'CkG
Cuts at: 1606 2418 2629 2692 2726 2895 3028 3218 3494
Size: 812 211 63 34 169 133 190 276
Cuts at: 3494 3553 4104 4781 5929 6174 1606
Size: 59 551 677 1148 245 2120
Fragments arranged by size:
2120 1148 812 677 551 276 245 211
190 169 133 63 59 34
NaeI GCC'GGC
Cuts at: 3617 3617
Size: 6688
NcoI C'CATG_G
Cuts at: 513 1065 3301 3718 513
Size: 552 2236 417 3483
Fragments arranged by size:
3483 2236 552 417
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6688
NgoMIV G'CCGG_C
Cuts at: 3615 3615
Size: 6688
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6688
NotI GC'GGCC_GC
Cuts at: 4135 4135
Size: 6688
NspI r_CATG'y
Cuts at: 1132 1229 4046 5591 1132
Size: 97 2817 1545 2229
Fragments arranged by size:
2817 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3223 1348
Size: 1875 4813
PciI A'CATG_T
Cuts at: 5587 5587
Size: 6688
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6688
PfoI T'CCnGG_A
Cuts at: 3533 3533
Size: 6688
PmeI GTTT'AAAC
Cuts at: 3992 3992
Size: 6688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3280 3312 4757 4789 6270 6302 3280
Size: 32 1445 32 1481 32 3666
Fragments arranged by size:
3666 1481 1445 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2458 3227 3354 2458
Size: 769 127 5792
Fragments arranged by size:
5792 769 127
PshAI GACnn'nnGTC
Cuts at: 1613 3460 1613
Size: 1847 4841
PsiI TTA'TAA
Cuts at: 4258 4258
Size: 6688
PspOMI G'GGCC_C
Cuts at: 2669 2669
Size: 6688
PstI C_TGCA'G
Cuts at: 830 2509 3637 4040 830
Size: 1679 1128 403 3478
Fragments arranged by size:
3478 1679 1128 403
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6688
PvuII CAG'CTG
Cuts at: 2418 2629 2895 2418
Size: 211 266 6211
Fragments arranged by size:
6211 266 211
SacI G_AGCT'C
Cuts at: 3428 3428
Size: 6688
SacII CC_GC'GG
Cuts at: 2693 2727 3219 3495 2693
Size: 34 492 276 5886
Fragments arranged by size:
5886 492 276 34
SalI G'TCGA_C
Cuts at: 1936 3795 3886 4030 1936
Size: 1859 91 144 4594
Fragments arranged by size:
4594 1859 144 91
SanDI GG'GwC_CC
Cuts at: 2458 2458
Size: 6688
SbfI CC_TGCA'GG
Cuts at: 4040 4040
Size: 6688
ScaI AGT'ACT
Cuts at: 4936 6648 4936
Size: 1712 4976
SfcI C'TryA_G
Cuts at: 826 1045 2505 3633 4036 5171 5852 6043 826
Size: 219 1460 1128 403 1135 681 191 1471
Fragments arranged by size:
1471 1460 1135 1128 681 403 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6688
SmaI CCC'GGG
Cuts at: 1872 3519 4015 1872
Size: 1647 496 4545
Fragments arranged by size:
4545 1647 496
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2151 2167 3418 4792 5693 5955
Size: 197 926 208 16 1251 1374 901 262
Cuts at: 5955 820
Size: 1553
Fragments arranged by size:
1553 1374 1251 926 901 262 208 197
16
SnaBI TAC'GTA
Cuts at: 493 3985 4479 493
Size: 3492 494 2702
Fragments arranged by size:
3492 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6688
SphI G_CATG'C
Cuts at: 1132 4046 1132
Size: 2914 3774
SspI AAT'ATT
Cuts at: 5 52 2579 4612 5
Size: 47 2527 2033 2081
Fragments arranged by size:
2527 2081 2033 47
StuI AGG'CCT
Cuts at: 1517 2111 2948 3382 1517
Size: 594 837 434 4823
Fragments arranged by size:
4823 837 594 434
StyI C'CwwG_G
Cuts at: 513 1065 2943 2967 3301 3718 4127 513
Size: 552 1878 24 334 417 409 3074
Fragments arranged by size:
3074 1878 552 417 409 334 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2458 3556 4715 4732 4885 5070 5515
Size: 1255 878 1098 1159 17 153 185 445
Cuts at: 5515 325
Size: 1498
Fragments arranged by size:
1498 1255 1159 1098 878 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2085 2754 4934
Size: 274 80 33 51 1443 108 669 2180
Cuts at: 4934 6646 96
Size: 1712 138
Fragments arranged by size:
2180 1712 1443 669 274 138 108 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2268 2300 3094 3126 6648 6680 2268
Size: 32 794 32 3522 32 2276
Fragments arranged by size:
3522 2276 794 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6688
XbaI T'CTAG_A
Cuts at: 4024 4024
Size: 6688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3299 3550 3299
Size: 251 6437
XhoI C'TCGA_G
Cuts at: 1943 2151 1943
Size: 208 6480
XmaI C'CCGG_G
Cuts at: 1870 3517 4013 1870
Size: 1647 496 4545
Fragments arranged by size:
4545 1647 496
XmnI GAAnn'nnTTC
Cuts at: 4817 4817
Size: 6688
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2682 3891 4496 276
Size: 53 83 186 936 1148 1209 605 2468
Fragments arranged by size:
2468 1209 1148 936 605 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI
SwaI