Restriction map for
pFN21ASDA1479
(Circular) (Six-base) MAPSORT of: pFN21ASDA1479.seq Check: 3523 from: 1 to: 7762
pFN21ASDA1479 7762 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3863 3863
Size: 7762
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5572 278
Size: 53 83 186 936 4036 2468
Fragments arranged by size:
4036 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2121 3885 4163 5105 1937
Size: 184 1764 278 942 4594
Fragments arranged by size:
4594 1764 942 278 184
Acc65I G'GTAC_C
Cuts at: 2432 5083 2432
Size: 2651 5111
AclI AA'CG_TT
Cuts at: 1563 5889 6262 1563
Size: 4326 373 3063
Fragments arranged by size:
4326 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 2151 3372 3732 4505 5825 7209 7477
Size: 483 36 1221 360 773 1320 1384 268
Cuts at: 7477 1632
Size: 1917
Fragments arranged by size:
1917 1384 1320 1221 773 483 360 268
36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7565
AflIII A'CryG_T
Cuts at: 1203 3900 4350 6661 7638 1203
Size: 2697 450 2311 977 1327
Fragments arranged by size:
2697 2311 1327 977 450
AgeI A'CCGG_T
Cuts at: 6593 6593
Size: 7762
AhdI GACnn_n'nnGTC
Cuts at: 6491 6491
Size: 7762
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7762
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3904 1184
Size: 2720 5042
AlwNI CAG_nnn'CTG
Cuts at: 2588 3077 3564 5505 7077 2588
Size: 489 487 1941 1572 3273
Fragments arranged by size:
3273 1941 1572 489 487
ApaLI G'TGCA_C
Cuts at: 5819 6975 5819
Size: 1156 6606
ApoI r'AATT_y
Cuts at: 1493 2214 3734 4735 5071 5297 5449 7558 1493
Size: 721 1520 1001 336 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 1001 721 336 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6316 160
Size: 872 5284 1606
Fragments arranged by size:
5284 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7762
AvaI C'yCGr_G
Cuts at: 1870 1943 3267 5087 1870
Size: 73 1324 1820 4545
Fragments arranged by size:
4545 1820 1324 73
AvrII C'CTAG_G
Cuts at: 4949 4949
Size: 7762
BamHI G'GATC_C
Cuts at: 3638 4564 5092 5459 3638
Size: 926 528 367 5941
Fragments arranged by size:
5941 926 528 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2432 5083 6538 618
Size: 325 217 627 645 2651 1455 1842
Fragments arranged by size:
2651 1842 1455 645 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3252 5081 1391
Size: 1861 1829 4072
Fragments arranged by size:
4072 1861 1829
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 3105 3477 3687 4190 928
Size: 971 124 1082 372 210 503 4500
Fragments arranged by size:
4500 1082 971 503 372 210 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5953 5987 1903
Size: 34 4016 34 3678
Fragments arranged by size:
4016 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5654 6864 5654
Size: 1210 6552
BclI T'GATC_A
Cuts at: 1548 4405 1548
Size: 2857 4905
BfrBI ATG'CAT
Cuts at: 2441 2441
Size: 7762
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2169 2585 6373
Size: 107 122 71 631 605 497 416 3788
Cuts at: 6373 136
Size: 1525
Fragments arranged by size:
3788 1525 631 605 497 416 122 107
71
BglII A'GATC_T
Cuts at: 7757 7757
Size: 7762
BlpI GC'TnA_GC
Cuts at: 5179 5179
Size: 7762
Bme1580I G_kGCm'C
Cuts at: 1790 5823 6979 1790
Size: 4033 1156 2573
Fragments arranged by size:
4033 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4353 4990 1318
Size: 3035 637 4090
Fragments arranged by size:
4090 3035 637
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2065 3943 4305
Size: 416 53 83 399 24 648 1878 362
Cuts at: 4305 4860 5493 6451 442
Size: 555 633 958 1753
Fragments arranged by size:
1878 1753 958 648 633 555 416 399
362 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2852 2904 3054 3813 3949 4116 4710 4897
Size: 1613 52 150 759 136 167 594 187
Cuts at: 4897 6422 1239
Size: 1525 2579
Fragments arranged by size:
2579 1613 1525 759 594 187 167 150
136 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5887 6752 7050 5887
Size: 865 298 6599
Fragments arranged by size:
6599 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3998 6425 882
Size: 617 2499 2427 2219
Fragments arranged by size:
2499 2427 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5059 5553 493
Size: 711 3855 494 2702
Fragments arranged by size:
3855 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3504 7756 3504
Size: 4252 3510
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2161 4970 5569 5951
Size: 53 83 186 936 628 2809 599 382
Cuts at: 5951 6586 275
Size: 635 1451
Fragments arranged by size:
2809 1451 936 635 628 599 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2146 6195 6593 6867 7014 7425 2146
Size: 4049 398 274 147 411 2483
Fragments arranged by size:
4049 2483 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7537
Fragments arranged by size:
7537 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7762
BseYI C'CCAG_C
Cuts at: 2820 6965 7461 2820
Size: 4145 496 3121
Fragments arranged by size:
4145 3121 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3472 1163
Size: 573 1736 5453
Fragments arranged by size:
5453 1736 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5212 5973 7001 1540
Size: 275 186 3211 761 1028 2301
Fragments arranged by size:
3211 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5823 5908 6979 5823
Size: 85 1071 6606
Fragments arranged by size:
6606 1071 85
BsmI GAATG_Cn'
Cuts at: 2378 2441 3863 5273 5366 2378
Size: 63 1422 1410 93 4774
Fragments arranged by size:
4774 1422 1410 93 63
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3252 5081 5823 5908 6979 1391
Size: 399 1462 1829 742 85 1071 2174
Fragments arranged by size:
2174 1829 1462 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7425 7425
Size: 7762
BspHI T'CATG_A
Cuts at: 5651 7381 5651
Size: 1730 6032
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3863 4065 5117 844
Size: 3019 202 1052 3489
Fragments arranged by size:
3489 3019 1052 202
BsrBI CCG'CTC
Cuts at: 1394 1823 5649 1394
Size: 429 3826 3507
Fragments arranged by size:
3826 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2179 3237 3867 6257 6431 65
Size: 1183 931 1058 630 2390 174 1396
Fragments arranged by size:
2390 1396 1183 1058 931 630 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4443 6406 6593 1700
Size: 110 2633 1963 187 2869
Fragments arranged by size:
2869 2633 1963 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7762
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7762
BssSI C'ACGA_G
Cuts at: 3606 5822 6834 3606
Size: 2216 1012 4534
Fragments arranged by size:
4534 2216 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3869 794
Size: 3075 4687
BstBI TT'CG_AA
Cuts at: 2007 6566 2007
Size: 4559 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7762
BstXI CCAn_nnnn'nTGG
Cuts at: 3298 3808 4111 4229 4669 3298
Size: 510 303 118 440 6391
Fragments arranged by size:
6391 510 440 303 118
BstYI r'GATC_y
Cuts at: 1972 2636 3638 3924 4564 5092 5459 5844 5861
Size: 664 1002 286 640 528 367 385 17
Cuts at: 5861 7313 7399 7411 7757 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1002 664 640 528 385 367
346 286 86 17 12
BstZ17I GTA'TAC
Cuts at: 3886 3886
Size: 7762
BtgI C'CryG_G
Cuts at: 513 1065 3766 4104 4222 4228 4569 4825 513
Size: 552 2701 338 118 6 341 256 3450
Fragments arranged by size:
3450 2701 552 341 338 256 118 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2619 4602 487
Size: 683 56 1393 1983 3647
Fragments arranged by size:
3647 1983 1393 683 56
BtsI GCAGTG_nn'
Cuts at: 3460 5278 6072 6092 3460
Size: 1818 794 20 5130
Fragments arranged by size:
5130 1818 794 20
ClaI AT'CG_AT
Cuts at: 1554 3171 4577 1554
Size: 1617 1406 4739
Fragments arranged by size:
4739 1617 1406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7727
DraI TTT'AAA
Cuts at: 5066 5066
Size: 7762
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3479 4007 6769 809
Size: 507 2163 528 2762 1802
Fragments arranged by size:
2762 2163 1802 528 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 3288 4225
Size: 54 1425 50 275 18 768 690 937
Cuts at: 4225 5209 6098 7727 8
Size: 984 889 1629 43
Fragments arranged by size:
1629 1425 984 937 889 768 690 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5209 1537
Size: 275 3397 4090
Fragments arranged by size:
4090 3397 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3417 4152 4196 4950 5692 1353
Size: 2064 735 44 754 742 3423
Fragments arranged by size:
3423 2064 754 742 735 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3934 4263 6349 6723 6869 387
Size: 1064 138 2345 329 2086 374 146 1280
Fragments arranged by size:
2345 2086 1280 1064 374 329 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2151 2852 2904 3054 3372 3732
Size: 393 483 36 701 52 150 318 360
Cuts at: 3732 3813 3949 4116 4505 4710 4897 5825 6422
Size: 81 136 167 389 205 187 928 597
Cuts at: 6422 7209 7477 1239
Size: 787 268 1524
Fragments arranged by size:
1524 928 787 701 597 483 393 389
360 318 268 205 187 167 150 136
81 52 36
EcoNI CCTnn'n_nnAGG
Cuts at: 4744 4744
Size: 7762
EcoO109I rG'GnC_Cy
Cuts at: 2827 4588 2827
Size: 1761 6001
EcoRI G'AATT_C
Cuts at: 3734 5071 5449 3734
Size: 1337 378 6047
Fragments arranged by size:
6047 1337 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5023 5023
Size: 7762
FspI TGC'GCA
Cuts at: 6268 6268
Size: 7762
HaeII r_GCGC'y
Cuts at: 1678 6909 1678
Size: 5231 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3768 3800 3926 3958 4085
Size: 32 135 32 2196 32 126 32 127
Cuts at: 4085 4117 4659 4691 4879 4911 6403 6435 6477
Size: 32 542 32 188 32 1492 32 42
Cuts at: 6477 6509 1373
Size: 32 2626
Fragments arranged by size:
2626 2196 1492 542 188 135 127 126
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2122 3675 5106 5352 669
Size: 988 281 184 1553 1431 246 3079
Fragments arranged by size:
3079 1553 1431 988 281 246 184
HindIII A'AGCT_T
Cuts at: 748 3153 4391 748
Size: 2405 1238 4119
Fragments arranged by size:
4119 2405 1238
HpaI GTT'AAC
Cuts at: 3675 5352 3675
Size: 1677 6085
KpnI G_GTAC'C
Cuts at: 2436 5087 2436
Size: 2651 5111
MfeI C'AATT_G
Cuts at: 5361 5361
Size: 7762
MluI A'CGCG_T
Cuts at: 7638 7638
Size: 7762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3477 3825 4049 6876 7060 1305
Size: 2172 348 224 2827 184 2007
Fragments arranged by size:
2827 2172 2007 348 224 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2600 4227 10
Size: 54 1425 343 768 1627 3545
Fragments arranged by size:
3545 1627 1425 768 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 3296 3819 3933 4109 4227 4494
Size: 648 1201 929 523 114 176 118 267
Cuts at: 4494 4538 5720 6079 6238 518
Size: 44 1182 359 159 2042
Fragments arranged by size:
2042 1201 1182 929 648 523 359 267
176 159 118 114 44
MspA1I CmG'CkG
Cuts at: 1606 2203 2824 5178 5855 7003 7248 1606
Size: 597 621 2354 677 1148 245 2120
Fragments arranged by size:
2354 2120 1148 677 621 597 245
NcoI C'CATG_G
Cuts at: 513 1065 3766 4104 4222 4228 4569 513
Size: 552 2701 338 118 6 341 3706
Fragments arranged by size:
3706 2701 552 341 338 118 6
NdeI CA'TA_TG
Cuts at: 387 2795 387
Size: 2408 5354
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7762
NotI GC'GGCC_GC
Cuts at: 5209 5209
Size: 7762
NsiI A_TGCA'T
Cuts at: 2443 2443
Size: 7762
NspI r_CATG'y
Cuts at: 1132 1229 3904 5120 6665 1132
Size: 97 2675 1216 1545 2229
Fragments arranged by size:
2675 2229 1545 1216 97
PasI CC'CwG_GG
Cuts at: 1348 3659 1348
Size: 2311 5451
PciI A'CATG_T
Cuts at: 3900 6661 3900
Size: 2761 5001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3122 4088 1108
Size: 2014 966 4782
Fragments arranged by size:
4782 2014 966
PfoI T'CCnGG_A
Cuts at: 2829 3177 4687 2829
Size: 348 1510 5904
Fragments arranged by size:
5904 1510 348
PmeI GTTT'AAAC
Cuts at: 5066 5066
Size: 7762
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 5831 5863 7344 7376 3239
Size: 32 2560 32 1481 32 3625
Fragments arranged by size:
3625 2560 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2827 4588 2827
Size: 1761 6001
PshAI GACnn'nnGTC
Cuts at: 1613 3269 1613
Size: 1656 6106
PsiI TTA'TAA
Cuts at: 3159 5332 3159
Size: 2173 5589
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3357 3389 3357
Size: 32 7730
PstI C_TGCA'G
Cuts at: 830 2916 4194 4902 5114 830
Size: 2086 1278 708 212 3478
Fragments arranged by size:
3478 2086 1278 708 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7762
PvuII CAG'CTG
Cuts at: 2203 2203
Size: 7762
SalI G'TCGA_C
Cuts at: 1936 2120 5104 1936
Size: 184 2984 4594
Fragments arranged by size:
4594 2984 184
SapI GCTCTTCn'nnn_
Cuts at: 4152 4196 4152
Size: 44 7718
SbfI CC_TGCA'GG
Cuts at: 4194 5114 4194
Size: 920 6842
ScaI AGT'ACT
Cuts at: 6010 7722 6010
Size: 1712 6050
SfcI C'TryA_G
Cuts at: 826 1045 2464 2912 4190 4898 5110 6245 6926
Size: 219 1419 448 1278 708 212 1135 681
Cuts at: 6926 7117 826
Size: 191 1471
Fragments arranged by size:
1471 1419 1278 1135 708 681 448 219
212 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7762
SgrAI Cr'CCGG_yG
Cuts at: 1700 4443 1700
Size: 2743 5019
SmaI CCC'GGG
Cuts at: 1872 5089 1872
Size: 3217 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5866 6767 7029 820
Size: 197 926 3923 901 262 1553
Fragments arranged by size:
3923 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5059 5553 493
Size: 4566 494 2702
Fragments arranged by size:
4566 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7762
SphI G_CATG'C
Cuts at: 1132 5120 1132
Size: 3988 3774
SspI AAT'ATT
Cuts at: 5 52 4030 5686 5
Size: 47 3978 1656 2081
Fragments arranged by size:
3978 2081 1656 47
StuI AGG'CCT
Cuts at: 1517 3121 1517
Size: 1604 6158
StyI C'CwwG_G
Cuts at: 513 1065 3116 3766 4104 4222 4228 4569 4949
Size: 552 2051 650 338 118 6 341 380
Cuts at: 4949 5201 513
Size: 252 3074
Fragments arranged by size:
3074 2051 650 552 380 341 338 252
118 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2618 2801 3250 4099 5789 5806 5959
Size: 1255 1038 183 449 849 1690 17 153
Cuts at: 5959 6144 6589 325
Size: 185 445 1498
Fragments arranged by size:
1690 1498 1255 1038 849 449 445 185
183 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6008 7720 96
Size: 274 80 33 51 1443 4031 1712 138
Fragments arranged by size:
4031 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4584 4616 4721 4753 4869 4901 7722 7754 4584
Size: 32 105 32 116 32 2821 32 4592
Fragments arranged by size:
4592 2821 116 105 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4972 1316
Size: 3656 4106
XbaI T'CTAG_A
Cuts at: 5098 5098
Size: 7762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3078 3641 4522 3078
Size: 563 881 6318
Fragments arranged by size:
6318 881 563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7762
XmaI C'CCGG_G
Cuts at: 1870 5087 1870
Size: 3217 4545
XmnI GAAnn'nnTTC
Cuts at: 5891 5891
Size: 7762
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5570 276
Size: 53 83 186 936 4036 2468
Fragments arranged by size:
4036 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI