Restriction map for
pFN21ASDA1465
(Circular) (Six-base) MAPSORT of: pFN21ASDA1465.seq Check: 3228 from: 1 to: 6964
pFN21ASDA1465 6964 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2244 4774 278
Size: 53 83 186 936 708 2530 2468
Fragments arranged by size:
2530 2468 936 708 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3078 4307 1937
Size: 1141 1229 4594
Fragments arranged by size:
4594 1229 1141
Acc65I G'GTAC_C
Cuts at: 3704 4285 3704
Size: 581 6383
AclI AA'CG_TT
Cuts at: 1563 5091 5464 1563
Size: 3528 373 3063
Fragments arranged by size:
3528 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2828 5027 6411 6679 1632
Size: 1196 2199 1384 268 1917
Fragments arranged by size:
2199 1917 1384 1196 268
AfeI AGC'GCT
Cuts at: 3395 3395
Size: 6964
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6767
AflIII A'CryG_T
Cuts at: 1203 3127 3435 5863 6840 1203
Size: 1924 308 2428 977 1327
Fragments arranged by size:
2428 1924 1327 977 308
AgeI A'CCGG_T
Cuts at: 2878 5795 2878
Size: 2917 4047
AhdI GACnn_n'nnGTC
Cuts at: 2190 5693 2190
Size: 3503 3461
AleI CACnn'nnGTG
Cuts at: 1166 2621 1166
Size: 1455 5509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2751 2786 1184
Size: 1567 35 5362
Fragments arranged by size:
5362 1567 35
AlwNI CAG_nnn'CTG
Cuts at: 4707 6279 4707
Size: 1572 5392
ApaI G_GGCC'C
Cuts at: 2041 3153 3554 3561 2041
Size: 1112 401 7 5444
Fragments arranged by size:
5444 1112 401 7
ApaLI G'TGCA_C
Cuts at: 2712 3090 5021 6177 2712
Size: 378 1931 1156 3499
Fragments arranged by size:
3499 1931 1156 378
ApoI r'AATT_y
Cuts at: 1493 4273 4499 4651 6760 1493
Size: 2780 226 152 2109 1697
Fragments arranged by size:
2780 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4230 5518 160
Size: 872 3198 1288 1606
Fragments arranged by size:
3198 1606 1288 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
AvaI C'yCGr_G
Cuts at: 1870 1943 2439 2619 2633 3260 3305 3587 3883
Size: 73 496 180 14 627 45 282 296
Cuts at: 3883 3977 4289 1870
Size: 94 312 4545
Fragments arranged by size:
4545 627 496 312 296 282 180 94
73 45 14
BamHI G'GATC_C
Cuts at: 4294 4661 4294
Size: 367 6597
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2155 2509 2516 2680 2762
Size: 325 217 627 368 354 7 164 82
Cuts at: 2762 2858 2995 3046 3107 3170 3704 3851 4199
Size: 96 137 51 61 63 534 147 348
Cuts at: 4199 4285 5740 618
Size: 86 1455 1842
Fragments arranged by size:
1842 1455 627 534 368 354 348 325
217 164 147 137 96 86 82 63
61 51 7
BanII G_rGCy'C
Cuts at: 1391 2041 2432 2640 3153 3459 3554 3561 3580
Size: 650 391 208 513 306 95 7 19
Cuts at: 3580 3783 4172 4283 1391
Size: 203 389 111 4072
Fragments arranged by size:
4072 650 513 391 389 306 208 203
111 95 19 7
BbeI G_GCGC'C
Cuts at: 2513 2999 3111 3174 3855 4203 2513
Size: 486 112 63 681 348 5274
Fragments arranged by size:
5274 681 486 348 112 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3366 3463 4065 928
Size: 971 1467 97 602 3827
Fragments arranged by size:
3827 1467 971 602 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2630 2664 3947 3981 5155 5189 1903
Size: 34 693 34 1283 34 1174 34 3678
Fragments arranged by size:
3678 1283 1174 693 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4856 6066 4856
Size: 1210 5754
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6964
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3068 5575 136
Size: 107 122 71 631 605 1396 2507 1525
Fragments arranged by size:
2507 1525 1396 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6959 6959
Size: 6964
BlpI GC'TnA_GC
Cuts at: 2531 4381 2531
Size: 1850 5114
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2716 3051 3094 3153 3554 3561 3843
Size: 251 675 335 43 59 401 7 282
Cuts at: 3843 5025 6181 1790
Size: 1182 1156 2573
Fragments arranged by size:
2573 1182 1156 675 401 335 282 251
59 43 7
BmgBI CAC'GTC
Cuts at: 1318 2256 3469 4036 1318
Size: 938 1213 567 4246
Fragments arranged by size:
4246 1213 938 567
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4129 4695 5653
Size: 416 53 83 399 24 2712 566 958
Cuts at: 5653 442
Size: 1753
Fragments arranged by size:
2712 1753 958 566 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6964
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2659 4186 2659
Size: 1527 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2961 5624 1239
Size: 1722 2663 2579
Fragments arranged by size:
2663 2579 1722
Bpu10I CC'TnA_GC
Cuts at: 2387 2387
Size: 6964
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2309 3443 5089 5954 6252 2309
Size: 1134 1646 865 298 3021
Fragments arranged by size:
3021 1646 1134 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3305 4068 5627 882
Size: 617 1806 763 1559 2219
Fragments arranged by size:
2219 1806 1559 763 617
BsaAI yAC'GTr
Cuts at: 493 1204 3751 4261 4755 493
Size: 711 2547 510 494 2702
Fragments arranged by size:
2702 2547 711 510 494
BsaBI GATnn'nnATC
Cuts at: 6958 6958
Size: 6964
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 2510 2996 3075
Size: 53 83 186 936 708 269 486 79
Cuts at: 3075 3108 3171 3483 3669 3852 4200 4771 5153
Size: 33 63 312 186 183 348 571 382
Cuts at: 5153 5788 275
Size: 635 1451
Fragments arranged by size:
1451 936 708 635 571 486 382 348
312 269 186 186 183 83 79 63
53 33
BsaWI w'CCGG_w
Cuts at: 2878 5397 5795 6069 6216 6627 2878
Size: 2519 398 274 147 411 3215
Fragments arranged by size:
3215 2519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2959 2989 4121 4151 1178
Size: 30 165 30 1556 30 1132 30 3991
Fragments arranged by size:
3991 1556 1132 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3344 3365 3401 4037 4115 4169 1186
Size: 2158 21 36 636 78 54 3981
Fragments arranged by size:
3981 2158 636 78 54 36 21
BseYI C'CCAG_C
Cuts at: 2720 3001 3103 3478 3546 6167 6663 2720
Size: 281 102 375 68 2621 496 3021
Fragments arranged by size:
3021 2621 496 375 281 102 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3542 3665 4058 1163
Size: 573 1806 123 393 4069
Fragments arranged by size:
4069 1806 573 393 123
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3701 4186 4414 5175 6203 1540
Size: 275 186 1700 485 228 761 1028 2301
Fragments arranged by size:
2301 1700 1028 761 485 275 228 186
BsiHKAI G_wGCw'C
Cuts at: 2618 2716 3094 3459 3783 5025 5110 6181 2618
Size: 98 378 365 324 1242 85 1071 3401
Fragments arranged by size:
3401 1242 1071 378 365 324 98 85
BsiWI C'GTAC_G
Cuts at: 2501 2501
Size: 6964
BsmI GAATG_Cn'
Cuts at: 3300 4475 4568 3300
Size: 1175 93 5696
Fragments arranged by size:
5696 1175 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3311 3311
Size: 6964
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2432 2618 2640 2716 3051 3094
Size: 399 251 391 186 22 76 335 43
Cuts at: 3094 3153 3459 3554 3561 3580 3783 3843 4172
Size: 59 306 95 7 19 203 60 329
Cuts at: 4172 4283 5025 5110 6181 1391
Size: 111 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 335 329 306
251 203 186 111 95 85 76 60
59 43 22 19 7
BspEI T'CCGG_A
Cuts at: 6627 6627
Size: 6964
BspHI T'CATG_A
Cuts at: 4853 6583 4853
Size: 1730 5234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2385 2475 4098 4319 844
Size: 1541 90 1623 221 3489
Fragments arranged by size:
3489 1623 1541 221 90
BsrBI CCG'CTC
Cuts at: 1394 1823 2889 4851 1394
Size: 429 1066 1962 3507
Fragments arranged by size:
3507 1962 1066 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2646 2840 5459 5633 65
Size: 1183 1398 194 2619 174 1396
Fragments arranged by size:
2619 1398 1396 1183 194 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2493 2683 2878 2992 5608 5795 1700
Size: 110 683 190 195 114 2616 187 2869
Fragments arranged by size:
2869 2616 683 195 190 187 114 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6964
BssHII G'CGCG_C
Cuts at: 1925 2391 2535 2773 3542 1925
Size: 466 144 238 769 5347
Fragments arranged by size:
5347 769 466 238 144
BssSI C'ACGA_G
Cuts at: 5024 6036 5024
Size: 1012 5952
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2453 2680 794
Size: 1659 227 5078
Fragments arranged by size:
5078 1659 227
BstBI TT'CG_AA
Cuts at: 2007 5768 2007
Size: 3761 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6964
BstYI r'GATC_y
Cuts at: 1972 2847 3366 4294 4661 5046 5063 6515 6601
Size: 875 519 928 367 385 17 1452 86
Cuts at: 6601 6613 6959 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 928 875 519 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3915 3915
Size: 6964
BtgI C'CryG_G
Cuts at: 513 1065 2289 2366 3688 513
Size: 552 1224 77 1322 3789
Fragments arranged by size:
3789 1322 1224 552 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2788 3472 3771 3928 4201 487
Size: 683 56 1562 684 299 157 273 3250
Fragments arranged by size:
3250 1562 684 683 299 273 157 56
BtsI GCAGTG_nn'
Cuts at: 2572 2768 2797 3648 3653 3813 4480 5274 5294
Size: 196 29 851 5 160 667 794 20
Cuts at: 5294 2572
Size: 4242
Fragments arranged by size:
4242 851 794 667 196 160 29 20
5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3111 3146 529
Size: 35 2547 35 4347
Fragments arranged by size:
4347 2547 35 35
DraI TTT'AAA
Cuts at: 4268 4268
Size: 6964
DraIII CAC_nnn'GTG
Cuts at: 2453 2453
Size: 6964
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5971 809
Size: 507 4655 1802
Fragments arranged by size:
4655 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2307 2812
Size: 54 1425 50 275 18 240 237 505
Cuts at: 2812 2990 3288 3698 3831 4411 5300 6929 8
Size: 178 298 410 133 580 889 1629 43
Fragments arranged by size:
1629 1425 889 580 505 410 298 275
240 237 178 133 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3698 4411 1537
Size: 275 1886 713 4090
Fragments arranged by size:
4090 1886 713 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4894 1353
Size: 3541 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2927 3398 3518 3588 5551 5925
Size: 1064 138 1338 471 120 70 1963 374
Cuts at: 5925 6071 387
Size: 146 1280
Fragments arranged by size:
1963 1338 1280 1064 471 374 146 138
120 70
EcoICRI GAG'CTC
Cuts at: 3457 3781 3457
Size: 324 6640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2828 2961 5027 5624 6411 6679 1239
Size: 393 1196 133 2066 597 787 268 1524
Fragments arranged by size:
2066 1524 1196 787 597 393 268 133
EcoNI CCTnn'n_nnAGG
Cuts at: 3290 3290
Size: 6964
EcoO109I rG'GnC_Cy
Cuts at: 2038 2229 3150 3196 3409 3550 3551 3919 4048
Size: 191 921 46 213 141 1 368 129
Cuts at: 4048 4087 2038
Size: 39 4915
Fragments arranged by size:
4915 921 368 213 191 141 129 46
39 1
EcoRI G'AATT_C
Cuts at: 4273 4651 4273
Size: 378 6586
FspI TGC'GCA
Cuts at: 2285 2381 2480 5470 2285
Size: 96 99 2990 3779
Fragments arranged by size:
3779 2990 99 96
FspAI rTGC'GCAy
Cuts at: 2285 2285
Size: 6964
HaeII r_GCGC'y
Cuts at: 1678 2060 2513 2999 3111 3174 3397 3731 3855
Size: 382 453 486 112 63 223 334 124
Cuts at: 3855 4203 6111 1678
Size: 348 1908 2531
Fragments arranged by size:
2531 1908 486 453 382 348 334 223
124 112 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2176 2208 3200 3232 3770
Size: 32 135 32 604 32 992 32 538
Cuts at: 3770 3802 4121 4153 5605 5637 5679 5711 1373
Size: 32 319 32 1452 32 42 32 2626
Fragments arranged by size:
2626 1452 992 604 538 319 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3673 4213 4308 4554 669
Size: 988 281 1735 540 95 246 3079
Fragments arranged by size:
3079 1735 988 540 281 246 95
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6964
HpaI GTT'AAC
Cuts at: 4554 4554
Size: 6964
KasI G'GCGC_C
Cuts at: 2509 2995 3107 3170 3851 4199 2509
Size: 486 112 63 681 348 5274
Fragments arranged by size:
5274 681 486 348 112 63
KpnI G_GTAC'C
Cuts at: 3708 4289 3708
Size: 581 6383
MfeI C'AATT_G
Cuts at: 4563 4563
Size: 6964
MluI A'CGCG_T
Cuts at: 3127 3435 6840 3127
Size: 308 3405 3251
Fragments arranged by size:
3405 3251 308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6078 6262 1305
Size: 4773 184 2007
Fragments arranged by size:
4773 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3290 3833 10
Size: 54 1425 343 1458 543 3141
Fragments arranged by size:
3141 1458 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 2733 4922 5281 5440 518
Size: 648 1455 112 2189 359 159 2042
Fragments arranged by size:
2189 2042 1455 648 359 159 112
MspA1I CmG'CkG
Cuts at: 1606 2401 3526 3535 3626 3690 4380 5057 6205
Size: 795 1125 9 91 64 690 677 1148
Cuts at: 6205 6450 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1125 795 690 677 245 91
64 9
NaeI GCC'GGC
Cuts at: 2994 2994
Size: 6964
NarI GG'CG_CC
Cuts at: 2510 2996 3108 3171 3852 4200 2510
Size: 486 112 63 681 348 5274
Fragments arranged by size:
5274 681 486 348 112 63
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6412
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6964
NgoMIV G'CCGG_C
Cuts at: 2992 2992
Size: 6964
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6964
NotI GC'GGCC_GC
Cuts at: 4411 4411
Size: 6964
NspI r_CATG'y
Cuts at: 1132 1229 4322 5867 1132
Size: 97 3093 1545 2229
Fragments arranged by size:
3093 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3554 4195 1348
Size: 2206 641 4117
Fragments arranged by size:
4117 2206 641
PciI A'CATG_T
Cuts at: 5863 5863
Size: 6964
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3286 3871 1108
Size: 2178 585 4201
Fragments arranged by size:
4201 2178 585
PmeI GTTT'AAAC
Cuts at: 4268 4268
Size: 6964
PmlI CAC'GTG
Cuts at: 3751 3751
Size: 6964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5033 5065 6546 6578 5033
Size: 32 1481 32 5419
Fragments arranged by size:
5419 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3409 4087 3409
Size: 678 6286
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6964
PsiI TTA'TAA
Cuts at: 4534 4534
Size: 6964
PspOMI G'GGCC_C
Cuts at: 2037 3149 3550 3557 2037
Size: 1112 401 7 5444
Fragments arranged by size:
5444 1112 401 7
PspXI vC'TCGA_Gb
Cuts at: 3977 3977
Size: 6964
PstI C_TGCA'G
Cuts at: 830 2400 2601 2676 4095 4316 830
Size: 1570 201 75 1419 221 3478
Fragments arranged by size:
3478 1570 1419 221 201 75
PvuI CG_AT'CG
Cuts at: 2001 4186 2001
Size: 2185 4779
PvuII CAG'CTG
Cuts at: 2401 3526 2401
Size: 1125 5839
SacI G_AGCT'C
Cuts at: 3459 3783 3459
Size: 324 6640
SacII CC_GC'GG
Cuts at: 3691 3691
Size: 6964
SalI G'TCGA_C
Cuts at: 1936 4306 1936
Size: 2370 4594
SanDI GG'GwC_CC
Cuts at: 3409 3409
Size: 6964
SbfI CC_TGCA'GG
Cuts at: 4316 4316
Size: 6964
ScaI AGT'ACT
Cuts at: 5212 6924 5212
Size: 1712 5252
SfcI C'TryA_G
Cuts at: 826 1045 2396 2597 2672 4091 4240 4312 5447
Size: 219 1351 201 75 1419 149 72 1135
Cuts at: 5447 6128 6319 826
Size: 681 191 1471
Fragments arranged by size:
1471 1419 1351 1135 681 219 201 191
149 75 72
SfoI GGC'GCC
Cuts at: 2511 2997 3109 3172 3853 4201 2511
Size: 486 112 63 681 348 5274
Fragments arranged by size:
5274 681 486 348 112 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6964
SmaI CCC'GGG
Cuts at: 1872 2441 2621 3589 3885 4291 1872
Size: 569 180 968 296 406 4545
Fragments arranged by size:
4545 968 569 406 296 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 3458 3977 5068 5969 6231
Size: 197 926 381 1134 519 1091 901 262
Cuts at: 6231 820
Size: 1553
Fragments arranged by size:
1553 1134 1091 926 901 519 381 262
197
SnaBI TAC'GTA
Cuts at: 493 4261 4755 493
Size: 3768 494 2702
Fragments arranged by size:
3768 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6964
SphI G_CATG'C
Cuts at: 1132 4322 1132
Size: 3190 3774
SspI AAT'ATT
Cuts at: 5 52 4888 5
Size: 47 4836 2081
Fragments arranged by size:
4836 2081 47
StuI AGG'CCT
Cuts at: 1517 2603 3285 1517
Size: 1086 682 5196
Fragments arranged by size:
5196 1086 682
StyI C'CwwG_G
Cuts at: 513 1065 3063 3225 3280 3291 4140 4403 513
Size: 552 1998 162 55 11 849 263 3074
Fragments arranged by size:
3074 1998 849 552 263 162 55 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3329 4991 5008 5161 5346 5791 325
Size: 1255 1749 1662 17 153 185 445 1498
Fragments arranged by size:
1749 1662 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4176 5210 6922
Size: 274 80 33 51 1443 2199 1034 1712
Cuts at: 6922 96
Size: 138
Fragments arranged by size:
2199 1712 1443 1034 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3000 3032 6924 6956 3000
Size: 32 3892 32 3008
Fragments arranged by size:
3892 3008 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6964
XbaI T'CTAG_A
Cuts at: 4300 4300
Size: 6964
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2985 4229 2985
Size: 1244 5720
XhoI C'TCGA_G
Cuts at: 1943 3977 1943
Size: 2034 4930
XmaI C'CCGG_G
Cuts at: 1870 2439 2619 3587 3883 4289 1870
Size: 569 180 968 296 406 4545
Fragments arranged by size:
4545 968 569 406 296 180
XmnI GAAnn'nnTTC
Cuts at: 5093 5093
Size: 6964
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2242 4772 276
Size: 53 83 186 936 708 2530 2468
Fragments arranged by size:
2530 2468 936 708 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BstXI
BstZ17I EcoRV FalI FseI NruI NsiI PacI PfoI
PsrI RsrII SapI SexAI SfiI SrfI SwaI