Restriction map for
pFN21ASDA1458
(Circular) (Six-base) MAPSORT of: pFN21ASDA1458.seq Check: 8282 from: 1 to: 6472
pFN21ASDA1458 6472 bp
With 182 enzymes: SgfI *
February 10, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4282 278
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3027 3815 1937
Size: 1090 788 4594
Fragments arranged by size:
4594 1090 788
Acc65I G'GTAC_C
Cuts at: 3793 3793
Size: 6472
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2781 3360 4535 5919 6187 1632
Size: 1149 579 1175 1384 268 1917
Fragments arranged by size:
1917 1384 1175 1149 579 268
AfeI AGC'GCT
Cuts at: 3583 3583
Size: 6472
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 5371 6348 1203
Size: 4168 977 1327
Fragments arranged by size:
4168 1327 977
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2916 3683 3722 1184
Size: 1732 767 39 3934
Fragments arranged by size:
3934 1732 767 39
AlwNI CAG_nnn'CTG
Cuts at: 2080 4215 5787 2080
Size: 2135 1572 2765
Fragments arranged by size:
2765 2135 1572
ApaI G_GGCC'C
Cuts at: 2160 2259 2270 2160
Size: 99 11 6362
Fragments arranged by size:
6362 99 11
ApaLI G'TGCA_C
Cuts at: 4529 5685 4529
Size: 1156 5316
ApoI r'AATT_y
Cuts at: 1493 2575 2719 3100 3328 3398 3781 4007 4159
Size: 1082 144 381 228 70 383 226 152
Cuts at: 4159 6268 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1082 383 381 228 226 152
144 70
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 2125 2214 2258 2269 3126 3797 1870
Size: 73 182 89 44 11 857 671 4545
Fragments arranged by size:
4545 857 671 182 89 73 44 11
BamHI G'GATC_C
Cuts at: 2822 3802 4169 2822
Size: 980 367 5125
Fragments arranged by size:
5125 980 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 3793 5248 618
Size: 325 217 627 354 1652 1455 1842
Fragments arranged by size:
1842 1652 1455 627 354 325 217
BanII G_rGCy'C
Cuts at: 1391 2160 2259 2270 3791 1391
Size: 769 99 11 1521 4072
Fragments arranged by size:
4072 1521 769 99 11
BbeI G_GCGC'C
Cuts at: 2145 2145
Size: 6472
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3254 928
Size: 971 1355 4146
Fragments arranged by size:
4146 1355 971
BbvCI CC'TCA_GC
Cuts at: 2114 2234 2114
Size: 120 6352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4663 4697 1903
Size: 34 2726 34 3678
Fragments arranged by size:
3678 2726 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4364 5574 4364
Size: 1210 5262
BclI T'GATC_A
Cuts at: 1548 2545 2968 1548
Size: 997 423 5052
Fragments arranged by size:
5052 997 423
BfrBI ATG'CAT
Cuts at: 3016 3016
Size: 6472
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2359 2385 5083
Size: 107 122 71 631 605 687 26 2698
Cuts at: 5083 136
Size: 1525
Fragments arranged by size:
2698 1525 687 631 605 122 107 71
26
BglII A'GATC_T
Cuts at: 6467 6467
Size: 6472
BlpI GC'TnA_GC
Cuts at: 3889 3889
Size: 6472
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2259 2270 3646 4533 5689 1790
Size: 370 99 11 1376 887 1156 2573
Fragments arranged by size:
2573 1376 1156 887 370 99 11
BmgBI CAC'GTC
Cuts at: 1318 2913 1318
Size: 1595 4877
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2672 3633 4203
Size: 416 53 83 399 24 1255 961 570
Cuts at: 4203 5161 442
Size: 958 1753
Fragments arranged by size:
1753 1255 961 958 570 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2318 1056
Size: 1262 5210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2393 3375 5132 1239
Size: 875 279 982 1757 2579
Fragments arranged by size:
2579 1757 982 875 279
Bpu10I CC'TnA_GC
Cuts at: 2114 2234 3084 2114
Size: 120 850 5502
Fragments arranged by size:
5502 850 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2549 4597 5462 5760 2549
Size: 2048 865 298 3261
Fragments arranged by size:
3261 2048 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5135 882
Size: 617 3636 2219
Fragments arranged by size:
3636 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3769 4263 493
Size: 711 2565 494 2702
Fragments arranged by size:
2702 2565 711 494
BsaBI GATnn'nnATC
Cuts at: 6466 6466
Size: 6472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 2207 2301 4279
Size: 53 83 186 936 609 65 94 1978
Cuts at: 4279 4661 5296 275
Size: 382 635 1451
Fragments arranged by size:
1978 1451 936 635 609 382 186 94
83 65 53
BsaWI w'CCGG_w
Cuts at: 4905 5303 5577 5724 6135 4905
Size: 398 274 147 411 5242
Fragments arranged by size:
5242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2021 2051 2291 2321 2366
Size: 30 165 30 618 30 240 30 45
Cuts at: 2366 2396 1178
Size: 30 5254
Fragments arranged by size:
5254 618 240 165 45 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2131 2185 2202 2408 2411 2414 2417 3508
Size: 945 54 17 206 3 3 3 1091
Cuts at: 3508 1186
Size: 4150
Fragments arranged by size:
4150 1091 945 206 54 17 3 3
3
BseYI C'CCAG_C
Cuts at: 2388 3566 3653 5675 6171 2388
Size: 1178 87 2022 496 2689
Fragments arranged by size:
2689 2022 1178 496 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 2497 1163
Size: 573 420 341 5138
Fragments arranged by size:
5138 573 420 341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3077 3922 4683 5711 1540
Size: 275 186 1076 845 761 1028 2301
Fragments arranged by size:
2301 1076 1028 845 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3548 4533 4618 5689 3548
Size: 985 85 1071 4331
Fragments arranged by size:
4331 1071 985 85
BsmI GAATG_Cn'
Cuts at: 3983 4076 3983
Size: 93 6379
BsmBI CGTCTCn'nnnn_
Cuts at: 2214 2919 2214
Size: 705 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2259 2270 3548 3646 3791 4533
Size: 399 370 99 11 1278 98 145 742
Cuts at: 4533 4618 5689 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1278 1071 742 399 370 145 99
98 85 11
BspEI T'CCGG_A
Cuts at: 6135 6135
Size: 6472
BspHI T'CATG_A
Cuts at: 4361 6091 4361
Size: 1730 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3607 3827 844
Size: 2763 220 3489
Fragments arranged by size:
3489 2763 220
BsrBI CCG'CTC
Cuts at: 1394 1823 2124 4359 1394
Size: 429 301 2235 3507
Fragments arranged by size:
3507 2235 429 301
BsrDI GCAATG_nn'
Cuts at: 65 1248 3235 3621 4967 5141 65
Size: 1183 1987 386 1346 174 1396
Fragments arranged by size:
1987 1396 1346 1183 386 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2144 2177 5116 5303 1700
Size: 110 334 33 2939 187 2869
Fragments arranged by size:
2939 2869 334 187 110 33
BsrGI T'GTAC_A
Cuts at: 96 2854 96
Size: 2758 3714
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 4532 5544 4532
Size: 1012 5460
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6472
BstBI TT'CG_AA
Cuts at: 2007 5276 2007
Size: 3269 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6472
BstXI CCAn_nnnn'nTGG
Cuts at: 2472 3470 2472
Size: 998 5474
BstYI r'GATC_y
Cuts at: 1972 2822 3701 3802 4169 4554 4571 6023 6109
Size: 850 879 101 367 385 17 1452 86
Cuts at: 6109 6121 6467 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 879 850 385 367 346 101
86 17 12
BstZ17I GTA'TAC
Cuts at: 3028 3028
Size: 6472
Bsu36I CC'TnA_GG
Cuts at: 3369 3369
Size: 6472
BtgI C'CryG_G
Cuts at: 513 1065 2022 3463 513
Size: 552 957 1441 3522
Fragments arranged by size:
3522 1441 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5733
Fragments arranged by size:
5733 683 56
BtsI GCAGTG_nn'
Cuts at: 2656 3988 4782 4802 2656
Size: 1332 794 20 4326
Fragments arranged by size:
4326 1332 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6437
DraI TTT'AAA
Cuts at: 3776 3776
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5479 809
Size: 507 4163 1802
Fragments arranged by size:
4163 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3919 4808 6437
Size: 54 1425 50 275 18 2089 889 1629
Cuts at: 6437 8
Size: 43
Fragments arranged by size:
2089 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3919 1537
Size: 275 2107 4090
Fragments arranged by size:
4090 2107 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 3438 4402 1353
Size: 930 1155 964 3423
Fragments arranged by size:
3423 1155 964 930
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2290 5059 5433 5579 387
Size: 1064 138 701 2769 374 146 1280
Fragments arranged by size:
2769 1280 1064 701 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2393 2781 3360 3375 4535 5132
Size: 393 482 279 388 579 15 1160 597
Cuts at: 5132 5919 6187 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1160 787 597 579 482 393 388
279 268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3390 3390
Size: 6472
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
FspI TGC'GCA
Cuts at: 4978 4978
Size: 6472
HaeII r_GCGC'y
Cuts at: 1678 2145 2241 2380 3585 5619 1678
Size: 467 96 139 1205 2034 2531
Fragments arranged by size:
2531 2034 1205 467 139 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2291 2323 2366
Size: 32 135 32 449 32 238 32 43
Cuts at: 2366 2398 5113 5145 5187 5219 1373
Size: 32 2715 32 42 32 2626
Fragments arranged by size:
2715 2626 449 238 135 43 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2035 3040 3298 3816 4062 669
Size: 988 281 97 1005 258 518 246 3079
Fragments arranged by size:
3079 1005 988 518 281 258 246 97
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6472
HpaI GTT'AAC
Cuts at: 2035 3040 4062 2035
Size: 1005 1022 4445
Fragments arranged by size:
4445 1022 1005
KasI G'GCGC_C
Cuts at: 2141 2141
Size: 6472
KpnI G_GTAC'C
Cuts at: 3797 3797
Size: 6472
MfeI C'AATT_G
Cuts at: 2817 4071 2817
Size: 1254 5218
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2064 3728 5586 5770 1305
Size: 759 1664 1858 184 2007
Fragments arranged by size:
2007 1858 1664 759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4650
Fragments arranged by size:
4650 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3665 4430 4789 4948 518
Size: 648 2499 765 359 159 2042
Fragments arranged by size:
2499 2042 765 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2356 3888 4565 5713 5958 1606
Size: 507 243 1532 677 1148 245 2120
Fragments arranged by size:
2120 1532 1148 677 507 245 243
NaeI GCC'GGC
Cuts at: 2146 2179 2146
Size: 33 6439
NarI GG'CG_CC
Cuts at: 2142 2142
Size: 6472
NcoI C'CATG_G
Cuts at: 513 1065 2022 3463 513
Size: 552 957 1441 3522
Fragments arranged by size:
3522 1441 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NgoMIV G'CCGG_C
Cuts at: 2144 2177 2144
Size: 33 6439
NheI G'CTAG_C
Cuts at: 1052 2314 1052
Size: 1262 5210
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NsiI A_TGCA'T
Cuts at: 3018 3018
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 3830 5375 1132
Size: 97 2601 1545 2229
Fragments arranged by size:
2601 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6472
PciI A'CATG_T
Cuts at: 5371 5371
Size: 6472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6472
PfoI T'CCnGG_A
Cuts at: 2713 2713
Size: 6472
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4541 4573 6054 6086 4541
Size: 32 1481 32 4927
Fragments arranged by size:
4927 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 2832 4042 2832
Size: 1210 5262
PspOMI G'GGCC_C
Cuts at: 2156 2255 2266 2156
Size: 99 11 6362
Fragments arranged by size:
6362 99 11
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3271 3303 3271
Size: 32 6440
PstI C_TGCA'G
Cuts at: 830 2076 3415 3604 3824 830
Size: 1246 1339 189 220 3478
Fragments arranged by size:
3478 1339 1246 220 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
PvuII CAG'CTG
Cuts at: 2113 2113
Size: 6472
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SapI GCTCTTCn'nnn_
Cuts at: 2283 3438 2283
Size: 1155 5317
SbfI CC_TGCA'GG
Cuts at: 3604 3824 3604
Size: 220 6252
ScaI AGT'ACT
Cuts at: 4720 6432 4720
Size: 1712 4760
SfcI C'TryA_G
Cuts at: 826 1045 2072 2691 2775 3411 3600 3820 4955
Size: 219 1027 619 84 636 189 220 1135
Cuts at: 4955 5636 5827 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 636 619 220 219
191 189 84
SfoI GGC'GCC
Cuts at: 2143 2143
Size: 6472
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 2260 3799 1872
Size: 388 1539 4545
Fragments arranged by size:
4545 1539 388
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2564 4576 5477 5739 820
Size: 197 926 621 2012 901 262 1553
Fragments arranged by size:
2012 1553 926 901 621 262 197
SnaBI TAC'GTA
Cuts at: 493 3769 4263 493
Size: 3276 494 2702
Fragments arranged by size:
3276 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SspI AAT'ATT
Cuts at: 5 52 4396 5
Size: 47 4344 2081
Fragments arranged by size:
4344 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6472
StyI C'CwwG_G
Cuts at: 513 1065 2022 2152 2226 3463 3911 513
Size: 552 957 130 74 1237 448 3074
Fragments arranged by size:
3074 1237 957 552 448 130 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3109 4499 4516 4669 4854 5299 325
Size: 1255 1529 1390 17 153 185 445 1498
Fragments arranged by size:
1529 1498 1390 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2854 4718 6430
Size: 274 80 33 51 1443 877 1864 1712
Cuts at: 6430 96
Size: 138
Fragments arranged by size:
1864 1712 1443 877 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6432 6464 6432
Size: 32 6440
Tth111I GACn'n_nGTC
Cuts at: 1316 3258 1316
Size: 1942 4530
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6472
XmaI C'CCGG_G
Cuts at: 1870 2258 3797 1870
Size: 388 1539 4545
Fragments arranged by size:
4545 1539 388
XmnI GAAnn'nnTTC
Cuts at: 4601 4601
Size: 6472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4280 276
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BplI BsiWI DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI PacI
PmlI PpuMI PspXI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI XcmI