Restriction map for
pFN21ASDA1439
(Circular) (Six-base) MAPSORT of: pFN21ASDA1439.seq Check: 8662 from: 1 to: 6256
pFN21ASDA1439 6256 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3599 1937
Size: 1662 4594
Acc65I G'GTAC_C
Cuts at: 3540 3577 3540
Size: 37 6219
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3011 4319 5703 5971 1632
Size: 1379 1308 1384 268 1917
Fragments arranged by size:
1917 1384 1379 1308 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 2741 3999 5571 2741
Size: 1258 1572 3426
Fragments arranged by size:
3426 1572 1258
ApaLI G'TGCA_C
Cuts at: 3302 4313 5469 3302
Size: 1011 1156 4089
Fragments arranged by size:
4089 1156 1011
ApoI r'AATT_y
Cuts at: 1493 2054 2749 3215 3565 3791 3943 6052 1493
Size: 561 695 466 350 226 152 2109 1697
Fragments arranged by size:
2109 1697 695 561 466 350 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 3581 1870
Size: 73 1638 4545
Fragments arranged by size:
4545 1638 73
BamHI G'GATC_C
Cuts at: 3499 3586 3953 3499
Size: 87 367 5802
Fragments arranged by size:
5802 367 87
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3379 3540 3577 5032 618
Size: 325 217 627 1592 161 37 1455 1842
Fragments arranged by size:
1842 1592 1455 627 325 217 161 37
BanII G_rGCy'C
Cuts at: 1391 2505 2843 3575 1391
Size: 1114 338 732 4072
Fragments arranged by size:
4072 1114 732 338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3099 3133 4447 4481 1903
Size: 34 1162 34 1314 34 3678
Fragments arranged by size:
3678 1314 1162 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4148 5358 3542
Size: 606 1210 4440
Fragments arranged by size:
4440 1210 606
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4867 136
Size: 107 122 71 631 605 3195 1525
Fragments arranged by size:
3195 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 3673 3673
Size: 6256
Bme1580I G_kGCm'C
Cuts at: 1790 3306 4317 5473 1790
Size: 1516 1011 1156 2573
Fragments arranged by size:
2573 1516 1156 1011
BmgBI CAC'GTC
Cuts at: 1318 2089 3405 3435 1318
Size: 771 1316 30 4139
Fragments arranged by size:
4139 1316 771 30
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3987 4945 442
Size: 416 53 83 399 24 2570 958 1753
Fragments arranged by size:
2570 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2422 4916 1239
Size: 1183 2494 2579
Fragments arranged by size:
2579 2494 1183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2840 4381 5246 5544 2840
Size: 1541 865 298 3552
Fragments arranged by size:
3552 1541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4919 882
Size: 617 3420 2219
Fragments arranged by size:
3420 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3553 4047 493
Size: 711 2349 494 2702
Fragments arranged by size:
2702 2349 711 494
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4063 4445 5080 275
Size: 53 83 186 936 2530 382 635 1451
Fragments arranged by size:
2530 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4689 5087 5361 5508 5919 4689
Size: 398 274 147 411 5026
Fragments arranged by size:
5026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3379 3409 1178
Size: 30 165 30 1976 30 4025
Fragments arranged by size:
4025 1976 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2824 2906 3031 1186
Size: 1638 82 125 4411
Fragments arranged by size:
4411 1638 125 82
BseYI C'CCAG_C
Cuts at: 2603 2822 3178 3455 5459 5955 2603
Size: 219 356 277 2004 496 2904
Fragments arranged by size:
2904 2004 496 356 277 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3441 1163
Size: 573 1705 3978
Fragments arranged by size:
3978 1705 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2843 3306 4317 4402 5473 2843
Size: 463 1011 85 1071 3626
Fragments arranged by size:
3626 1071 1011 463 85
BsmI GAATG_Cn'
Cuts at: 2976 3767 3860 2976
Size: 791 93 5372
Fragments arranged by size:
5372 791 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3197 3197
Size: 6256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2505 2843 3306 3575 4317 4402 5473
Size: 399 715 338 463 269 742 85 1071
Cuts at: 5473 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 715 463 399 338 269
85
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 3611 844
Size: 1283 1484 3489
Fragments arranged by size:
3489 1484 1283
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 4143 1394
Size: 429 212 2108 3507
Fragments arranged by size:
3507 2108 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 2811 4751 4925 65
Size: 1183 1563 1940 174 1396
Fragments arranged by size:
1940 1563 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3376 4900 5087 1700
Size: 110 1566 1524 187 2869
Fragments arranged by size:
2869 1566 1524 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 2007 5060 2007
Size: 3053 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6256
BstXI CCAn_nnnn'nTGG
Cuts at: 3158 3158
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 3499 3586 3953 4338 4355 5807 5893 5905
Size: 1527 87 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1527 1452 385 367 346 87 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3198 3198
Size: 6256
BtgI C'CryG_G
Cuts at: 513 1065 3494 513
Size: 552 2429 3275
Fragments arranged by size:
3275 2429 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 3772 4566 4586 3772
Size: 794 20 5442
Fragments arranged by size:
5442 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 2434 3560 2434
Size: 1126 5130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2672 5263 809
Size: 507 1356 2591 1802
Fragments arranged by size:
2591 1802 1356 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3357 3703 4592
Size: 54 1425 50 275 18 1527 346 889
Cuts at: 4592 6221 8
Size: 1629 43
Fragments arranged by size:
1629 1527 1425 889 346 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 3129 4186 1353
Size: 819 957 1057 3423
Fragments arranged by size:
3423 1057 957 819
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2372 4843 5217 5363 387
Size: 1064 138 783 2471 374 146 1280
Fragments arranged by size:
2471 1280 1064 783 374 146 138
EcoICRI GAG'CTC
Cuts at: 2841 2841
Size: 6256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2422 3011 4319 4916 5703 5971 1239
Size: 393 790 589 1308 597 787 268 1524
Fragments arranged by size:
1524 1308 790 787 597 589 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2384 2384
Size: 6256
EcoO109I rG'GnC_Cy
Cuts at: 3089 3089
Size: 6256
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
EcoRV GAT'ATC
Cuts at: 2269 2269
Size: 6256
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 2470 2854 2920 5403 1678
Size: 792 384 66 2483 2531
Fragments arranged by size:
2531 2483 792 384 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4897 4929 4971 5003 1373
Size: 32 135 32 3325 32 42 32 2626
Fragments arranged by size:
3325 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3600 3846 669
Size: 988 281 1470 192 246 3079
Fragments arranged by size:
3079 1470 988 281 246 192
HindIII A'AGCT_T
Cuts at: 748 3505 748
Size: 2757 3499
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KpnI G_GTAC'C
Cuts at: 3544 3581 3544
Size: 37 6219
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2296 2364 2388 2536 2979 3005 3247 3422
Size: 991 68 24 148 443 26 242 175
Cuts at: 3422 3469 5370 5554 1305
Size: 47 1901 184 2007
Fragments arranged by size:
2007 1901 991 443 242 184 175 148
68 47 26 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3359 10
Size: 54 1425 343 1527 2907
Fragments arranged by size:
2907 1527 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3326 4214 4573 4732 518
Size: 648 2160 888 359 159 2042
Fragments arranged by size:
2160 2042 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2446 3672 4349 5497 5742 1606
Size: 840 1226 677 1148 245 2120
Fragments arranged by size:
2120 1226 1148 840 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5704
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2576 3110 3614 5159 1132
Size: 97 1347 534 504 1545 2229
Fragments arranged by size:
2229 1545 1347 534 504 97
PasI CC'CwG_GG
Cuts at: 1348 3046 1348
Size: 1698 4558
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2645 2930 1108
Size: 1537 285 4434
Fragments arranged by size:
4434 1537 285
PfoI T'CCnGG_A
Cuts at: 2661 2797 2661
Size: 136 6120
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3089 3089
Size: 6256
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PstI C_TGCA'G
Cuts at: 830 2124 2737 3608 830
Size: 1294 613 871 3478
Fragments arranged by size:
3478 1294 871 613
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
SacI G_AGCT'C
Cuts at: 2843 2843
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SbfI CC_TGCA'GG
Cuts at: 2124 3608 2124
Size: 1484 4772
ScaI AGT'ACT
Cuts at: 2455 4504 6216 2455
Size: 2049 1712 2495
Fragments arranged by size:
2495 2049 1712
SfcI C'TryA_G
Cuts at: 826 1045 2120 2733 2785 3604 4739 5420 5611
Size: 219 1075 613 52 819 1135 681 191
Cuts at: 5611 826
Size: 1471
Fragments arranged by size:
1471 1135 1075 819 681 613 219 191
52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 3583 1872
Size: 1711 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2855 4360 5261 5523 820
Size: 197 926 912 1505 901 262 1553
Fragments arranged by size:
1553 1505 926 912 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3553 4047 493
Size: 3060 494 2702
Fragments arranged by size:
3060 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 2576 3110 3614 1132
Size: 1444 534 504 3774
Fragments arranged by size:
3774 1444 534 504
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 3364 1517
Size: 1847 4409
StyI C'CwwG_G
Cuts at: 513 1065 2911 3292 3695 513
Size: 552 1846 381 403 3074
Fragments arranged by size:
3074 1846 552 403 381
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 3157 3192 3469 4283 4300 4453
Size: 1255 480 1097 35 277 814 17 153
Cuts at: 4453 4638 5083 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1097 814 480 445 277 185
153 35 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2453 4502 6214
Size: 274 80 33 51 1443 476 2049 1712
Cuts at: 6214 96
Size: 138
Fragments arranged by size:
2049 1712 1443 476 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3132 3164 3379 3411 6216 6248 3132
Size: 32 215 32 2805 32 3140
Fragments arranged by size:
3140 2805 215 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3532 1316
Size: 2216 4040
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2642 2642
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 3581 1870
Size: 1711 4545
XmnI GAAnn'nnTTC
Cuts at: 2076 4385 2076
Size: 2309 3947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI