Restriction map for
pFN21ASDA1436
(Circular) (Six-base) MAPSORT of: pFN21ASDA1436.seq Check: 9304 from: 1 to: 7366
pFN21ASDA1436 7366 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2949 5176 278
Size: 53 83 186 936 1413 2227 2468
Fragments arranged by size:
2468 2227 1413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4709 1937
Size: 2772 4594
Acc65I G'GTAC_C
Cuts at: 4687 4687
Size: 7366
AclI AA'CG_TT
Cuts at: 1563 5493 5866 1563
Size: 3930 373 3063
Fragments arranged by size:
3930 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 2913 2956 5429 6813 7081 1632
Size: 708 573 43 2473 1384 268 1917
Fragments arranged by size:
2473 1917 1384 708 573 268 43
AfeI AGC'GCT
Cuts at: 2663 2663
Size: 7366
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7169
AflIII A'CryG_T
Cuts at: 1203 3144 3510 6265 7242 1203
Size: 1941 366 2755 977 1327
Fragments arranged by size:
2755 1941 1327 977 366
AgeI A'CCGG_T
Cuts at: 6197 6197
Size: 7366
AhdI GACnn_n'nnGTC
Cuts at: 6095 6095
Size: 7366
AleI CACnn'nnGTG
Cuts at: 1166 2837 1166
Size: 1671 5695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7366
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 3917 3949 2237
Size: 32 1648 32 5654
Fragments arranged by size:
5654 1648 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3177 5109 6681 3177
Size: 1932 1572 3862
Fragments arranged by size:
3862 1932 1572
ApaI G_GGCC'C
Cuts at: 2468 3472 2468
Size: 1004 6362
ApaLI G'TGCA_C
Cuts at: 2582 3041 4223 5423 6579 2582
Size: 459 1182 1200 1156 3369
Fragments arranged by size:
3369 1200 1182 1156 459
ApoI r'AATT_y
Cuts at: 1493 4346 4675 4901 5053 7162 1493
Size: 2853 329 226 152 2109 1697
Fragments arranged by size:
2853 2109 1697 329 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5920 160
Size: 872 4888 1606
Fragments arranged by size:
4888 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7366
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2641 2727 2954 3275 4691 1870
Size: 73 95 603 86 227 321 1416 4545
Fragments arranged by size:
4545 1416 603 321 227 95 86 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2746 2779 2746
Size: 33 7333
BamHI G'GATC_C
Cuts at: 3525 4183 4696 5063 3525
Size: 658 513 367 5828
Fragments arranged by size:
5828 658 513 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 3157 4687 6142 618
Size: 325 217 627 241 1129 1530 1455 1842
Fragments arranged by size:
1842 1530 1455 1129 627 325 241 217
BanII G_rGCy'C
Cuts at: 1391 2334 2468 2514 2734 2753 3472 4557 4685
Size: 943 134 46 220 19 719 1085 128
Cuts at: 4685 1391
Size: 4072
Fragments arranged by size:
4072 1085 943 719 220 134 128 46
19
BbeI G_GCGC'C
Cuts at: 2032 2032
Size: 7366
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3195 3552 3616 4254 928
Size: 971 1296 357 64 638 4040
Fragments arranged by size:
4040 1296 971 638 357 64
BbvCI CC'TCA_GC
Cuts at: 4319 4319
Size: 7366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2029 2063 3395 3429 5557 5591 1903
Size: 34 92 34 1332 34 2128 34 3678
Fragments arranged by size:
3678 2128 1332 92 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4524 5258 6468 4524
Size: 734 1210 5422
Fragments arranged by size:
5422 1210 734
BclI T'GATC_A
Cuts at: 1548 4140 1548
Size: 2592 4774
BfrBI ATG'CAT
Cuts at: 3887 3887
Size: 7366
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5977 136
Size: 107 122 71 631 605 4305 1525
Fragments arranged by size:
4305 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4135 7361 4135
Size: 3226 4140
BlpI GC'TnA_GC
Cuts at: 4783 4783
Size: 7366
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2468 2586 2874 3045 3160 3472 4227
Size: 342 336 118 288 171 115 312 755
Cuts at: 4227 5427 6583 1790
Size: 1200 1156 2573
Fragments arranged by size:
2573 1200 1156 755 342 336 312 288
171 118 115
BmgBI CAC'GTC
Cuts at: 1318 2680 2964 1318
Size: 1362 284 5720
Fragments arranged by size:
5720 1362 284
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5097 6055 442
Size: 416 53 83 399 24 3680 958 1753
Fragments arranged by size:
3680 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7366
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4477 4477
Size: 7366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2215 3796 3890 4346 6026 1239
Size: 976 1581 94 456 1680 2579
Fragments arranged by size:
2579 1680 1581 976 456 94
Bpu10I CC'TnA_GC
Cuts at: 2492 4319 2492
Size: 1827 5539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5491 6356 6654 5491
Size: 865 298 6203
Fragments arranged by size:
6203 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3967 4008 6029 882
Size: 617 2468 41 2021 2219
Fragments arranged by size:
2468 2219 2021 617 41
BsaAI yAC'GTr
Cuts at: 493 1204 2461 3145 4663 5157 493
Size: 711 1257 684 1518 494 2702
Fragments arranged by size:
2702 1518 1257 711 684 494
BsaBI GATnn'nnATC
Cuts at: 7360 7360
Size: 7366
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 2322 2706 2946
Size: 53 83 186 936 496 293 384 240
Cuts at: 2946 4530 5173 5555 6190 275
Size: 1584 643 382 635 1451
Fragments arranged by size:
1584 1451 936 643 635 496 384 382
293 240 186 83 53
BsaWI w'CCGG_w
Cuts at: 3522 5799 6197 6471 6618 7029 3522
Size: 2277 398 274 147 411 3859
Fragments arranged by size:
3859 2277 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2735 2765 3917
Size: 30 165 30 834 30 468 30 1152
Cuts at: 3917 3947 4568 4598 1178
Size: 30 621 30 3946
Fragments arranged by size:
3946 1152 834 621 468 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3469 1186
Size: 2283 5083
BseYI C'CCAG_C
Cuts at: 2174 2257 2262 2387 2468 3208 3603 6569 7065
Size: 83 5 125 81 740 395 2966 496
Cuts at: 7065 2174
Size: 2475
Fragments arranged by size:
2966 2475 740 496 395 125 83 81
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 2450 2512 2537 3941 1163
Size: 573 705 9 62 25 1404 4588
Fragments arranged by size:
4588 1404 705 573 62 25 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4816 5577 6605 1540
Size: 275 186 2815 761 1028 2301
Fragments arranged by size:
2815 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2334 2586 3045 4227 4257 5427 5512 6583 2334
Size: 252 459 1182 30 1170 85 1071 3117
Fragments arranged by size:
3117 1182 1170 1071 459 252 85 30
BsmI GAATG_Cn'
Cuts at: 3265 4105 4877 4970 3265
Size: 840 772 93 5661
Fragments arranged by size:
5661 840 772 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2334 2468 2514 2586 2734 2753
Size: 399 342 202 134 46 72 148 19
Cuts at: 2753 2874 3045 3160 3472 4227 4257 4557 4685
Size: 121 171 115 312 755 30 300 128
Cuts at: 4685 5427 5512 6583 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 755 742 399 342 312 300
202 171 148 134 128 121 115 85
72 46 30 19
BspEI T'CCGG_A
Cuts at: 3522 7029 3522
Size: 3507 3859
BspHI T'CATG_A
Cuts at: 5255 6985 5255
Size: 1730 5636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2422 2622 4721 844
Size: 1578 200 2099 3489
Fragments arranged by size:
3489 2099 1578 200
BsrBI CCG'CTC
Cuts at: 1394 1823 2278 5253 1394
Size: 429 455 2975 3507
Fragments arranged by size:
3507 2975 455 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5861 6035 65
Size: 1183 4613 174 1396
Fragments arranged by size:
4613 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3378 6010 6197 1700
Size: 110 1568 2632 187 2869
Fragments arranged by size:
2869 2632 1568 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7366
BssHII G'CGCG_C
Cuts at: 1925 2472 1925
Size: 547 6819
BssSI C'ACGA_G
Cuts at: 2624 5426 6438 2624
Size: 2802 1012 3552
Fragments arranged by size:
3552 2802 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7366
BstBI TT'CG_AA
Cuts at: 2007 6170 2007
Size: 4163 3203
BstEII G'GTnAC_C
Cuts at: 1184 3397 1184
Size: 2213 5153
BstXI CCAn_nnnn'nTGG
Cuts at: 3301 4385 3301
Size: 1084 6282
BstYI r'GATC_y
Cuts at: 1972 2295 3525 4135 4183 4455 4696 5063 5448
Size: 323 1230 610 48 272 241 367 385
Cuts at: 5448 5465 6917 7003 7015 7361 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1230 610 385 367 346 323
272 241 86 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 4078 4078
Size: 7366
BtgI C'CryG_G
Cuts at: 513 1065 2022 2325 2587 2694 2820 2835 4412
Size: 552 957 303 262 107 126 15 1577
Cuts at: 4412 4542 513
Size: 130 3337
Fragments arranged by size:
3337 1577 957 552 303 262 130 126
107 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6627
Fragments arranged by size:
6627 683 56
BtsI GCAGTG_nn'
Cuts at: 2529 4882 5676 5696 2529
Size: 2353 794 20 4199
Fragments arranged by size:
4199 2353 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7366
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4577 4612 529
Size: 35 4013 35 3283
Fragments arranged by size:
4013 3283 35 35
DraI TTT'AAA
Cuts at: 4670 4670
Size: 7366
DraIII CAC_nnn'GTG
Cuts at: 3187 3187
Size: 7366
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4000 6373 809
Size: 507 2684 2373 1802
Fragments arranged by size:
2684 2373 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2445 2595 4813
Size: 54 1425 50 275 18 615 150 2218
Cuts at: 4813 5702 7331 8
Size: 889 1629 43
Fragments arranged by size:
2218 1629 1425 889 615 275 150 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4813 1537
Size: 275 3001 4090
Fragments arranged by size:
4090 3001 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3320 4304 5296 1353
Size: 1967 984 992 3423
Fragments arranged by size:
3423 1967 992 984
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2323 2530 2617 5953 6327 6473
Size: 1064 138 734 207 87 3336 374 146
Cuts at: 6473 387
Size: 1280
Fragments arranged by size:
3336 1280 1064 734 374 207 146 138
87
EcoICRI GAG'CTC
Cuts at: 2332 2332
Size: 7366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2340 2913 2956 3796 3890 4346
Size: 393 583 125 573 43 840 94 456
Cuts at: 4346 5429 6026 6813 7081 1239
Size: 1083 597 787 268 1524
Fragments arranged by size:
1524 1083 840 787 597 583 573 456
393 268 125 94 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2501 2974 4241 4297 2501
Size: 473 1267 56 5570
Fragments arranged by size:
5570 1267 473 56
EcoO109I rG'GnC_Cy
Cuts at: 2465 3271 4082 2465
Size: 806 811 5749
Fragments arranged by size:
5749 811 806
EcoRI G'AATT_C
Cuts at: 4346 4675 5053 4346
Size: 329 378 6659
Fragments arranged by size:
6659 378 329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3640 3718 4534 3640
Size: 78 816 6472
Fragments arranged by size:
6472 816 78
FspI TGC'GCA
Cuts at: 5872 5872
Size: 7366
HaeII r_GCGC'y
Cuts at: 1678 2032 2060 2570 2665 4633 6513 1678
Size: 354 28 510 95 1968 1880 2531
Fragments arranged by size:
2531 1968 1880 510 354 95 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2735 2767 3068 3100 3185
Size: 32 135 32 1163 32 301 32 85
Cuts at: 3185 3217 3611 3643 4103 4125 4135 4157 6007
Size: 32 394 32 460 22 10 22 1850
Cuts at: 6007 6039 6081 6113 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1850 1163 460 394 301 135 85
42 32 32 32 32 32 32 32
32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3640 4710 4956 669
Size: 988 281 1702 1070 246 3079
Fragments arranged by size:
3079 1702 1070 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7366
HpaI GTT'AAC
Cuts at: 3640 4956 3640
Size: 1316 6050
KasI G'GCGC_C
Cuts at: 2028 2028
Size: 7366
KpnI G_GTAC'C
Cuts at: 4691 4691
Size: 7366
MfeI C'AATT_G
Cuts at: 4965 4965
Size: 7366
MluI A'CGCG_T
Cuts at: 7242 7242
Size: 7366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6480 6664 1305
Size: 5175 184 2007
Fragments arranged by size:
5175 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5544
Fragments arranged by size:
5544 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 3075 4646 5324 5683 5842 518
Size: 648 1671 238 1571 678 359 159 2042
Fragments arranged by size:
2042 1671 1571 678 648 359 238 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2266 2551 2589 2867 3212 3466 3592
Size: 441 219 285 38 278 345 254 126
Cuts at: 3592 3603 3984 4782 5459 6607 6852 1606
Size: 11 381 798 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 677 441 381 345 285
278 254 245 219 126 38 11
NarI GG'CG_CC
Cuts at: 2029 2029
Size: 7366
NcoI C'CATG_G
Cuts at: 513 1065 2022 4412 513
Size: 552 957 2390 3467
Fragments arranged by size:
3467 2390 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7366
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7366
NotI GC'GGCC_GC
Cuts at: 4813 4813
Size: 7366
NsiI A_TGCA'T
Cuts at: 3889 3889
Size: 7366
NspI r_CATG'y
Cuts at: 1132 1229 3309 4724 6269 1132
Size: 97 2080 1415 1545 2229
Fragments arranged by size:
2229 2080 1545 1415 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7366
PciI A'CATG_T
Cuts at: 6265 6265
Size: 7366
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3097 1108
Size: 1989 5377
PfoI T'CCnGG_A
Cuts at: 4295 4295
Size: 7366
PmeI GTTT'AAAC
Cuts at: 4670 4670
Size: 7366
PmlI CAC'GTG
Cuts at: 2461 3145 2461
Size: 684 6682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2237 2269 2618 2650 3917 3949 5435 5467 6948
Size: 32 349 32 1267 32 1486 32 1481
Cuts at: 6948 6980 2237
Size: 32 2623
Fragments arranged by size:
2623 1486 1481 1267 349 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 3271 4082 3271
Size: 811 6555
PshAI GACnn'nnGTC
Cuts at: 1613 3959 1613
Size: 2346 5020
PsiI TTA'TAA
Cuts at: 4478 4936 4478
Size: 458 6908
PspOMI G'GGCC_C
Cuts at: 2464 3468 2464
Size: 1004 6362
PspXI vC'TCGA_Gb
Cuts at: 2038 2038
Size: 7366
PstI C_TGCA'G
Cuts at: 830 2286 4583 4718 830
Size: 1456 2297 135 3478
Fragments arranged by size:
3478 2297 1456 135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7366
PvuII CAG'CTG
Cuts at: 2266 2867 3212 3603 2266
Size: 601 345 391 6029
Fragments arranged by size:
6029 601 391 345
SacI G_AGCT'C
Cuts at: 2334 2334
Size: 7366
SacII CC_GC'GG
Cuts at: 2590 2590
Size: 7366
SalI G'TCGA_C
Cuts at: 1936 4708 1936
Size: 2772 4594
SapI GCTCTTCn'nnn_
Cuts at: 4304 4304
Size: 7366
SbfI CC_TGCA'GG
Cuts at: 4718 4718
Size: 7366
ScaI AGT'ACT
Cuts at: 5614 7326 5614
Size: 1712 5654
SexAI A'CCwGG_T
Cuts at: 3246 3246
Size: 7366
SfcI C'TryA_G
Cuts at: 826 1045 2282 2755 3367 3537 4579 4714 5849
Size: 219 1237 473 612 170 1042 135 1135
Cuts at: 5849 6530 6721 826
Size: 681 191 1471
Fragments arranged by size:
1471 1237 1135 1042 681 612 473 219
191 170 135
SfoI GGC'GCC
Cuts at: 2030 2030
Size: 7366
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7366
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7366
SmaI CCC'GGG
Cuts at: 1872 2643 2729 3277 4693 1872
Size: 771 86 548 1416 4545
Fragments arranged by size:
4545 1416 771 548 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 5470 6371 6633 820
Size: 197 926 95 3432 901 262 1553
Fragments arranged by size:
3432 1553 926 901 262 197 95
SnaBI TAC'GTA
Cuts at: 493 4663 5157 493
Size: 4170 494 2702
Fragments arranged by size:
4170 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7366
SphI G_CATG'C
Cuts at: 1132 3309 4724 1132
Size: 2177 1415 3774
Fragments arranged by size:
3774 2177 1415
SspI AAT'ATT
Cuts at: 5 52 3754 5290 5
Size: 47 3702 1536 2081
Fragments arranged by size:
3702 2081 1536 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7366
StyI C'CwwG_G
Cuts at: 513 1065 2022 2362 3267 4412 4805 513
Size: 552 957 340 905 1145 393 3074
Fragments arranged by size:
3074 1145 957 905 552 393 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2949 3209 3595 4405 4523 5393 5410
Size: 1255 1369 260 386 810 118 870 17
Cuts at: 5410 5563 5748 6193 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1369 1255 870 810 445 386 260
185 153 118 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3831 5612 7324
Size: 274 80 33 51 1443 1854 1781 1712
Cuts at: 7324 96
Size: 138
Fragments arranged by size:
1854 1781 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7326 7358 7326
Size: 32 7334
Tth111I GACn'n_nGTC
Cuts at: 1316 2965 1316
Size: 1649 5717
XbaI T'CTAG_A
Cuts at: 4702 4702
Size: 7366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2110 2323 4434 2029
Size: 81 213 2111 4961
Fragments arranged by size:
4961 2111 213 81
XhoI C'TCGA_G
Cuts at: 1943 2038 1943
Size: 95 7271
XmaI C'CCGG_G
Cuts at: 1870 2641 2727 3275 4691 1870
Size: 771 86 548 1416 4545
Fragments arranged by size:
4545 1416 771 548 86
XmnI GAAnn'nnTTC
Cuts at: 4505 5495 4505
Size: 990 6376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2947 5174 276
Size: 53 83 186 936 1413 2227 2468
Fragments arranged by size:
2468 2227 1413 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BsiWI BsmBI BstZ17I EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PsrI RsrII SanDI
SfiI SrfI SwaI