Restriction map for
pFN21ASDA1433
(Circular) (Six-base) MAPSORT of: pFN21ASDA1433.seq Check: 1116 from: 1 to: 6646
pFN21ASDA1433 6646 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4456 278
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3989 1937
Size: 2052 4594
Acc65I G'GTAC_C
Cuts at: 3967 3967
Size: 6646
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 2424 4709 6093 6361 1632
Size: 602 190 2285 1384 268 1917
Fragments arranged by size:
2285 1917 1384 602 268 190
AfeI AGC'GCT
Cuts at: 2537 2537
Size: 6646
AflII C'TTAA_G
Cuts at: 820 1017 2270 820
Size: 197 1253 5196
Fragments arranged by size:
5196 1253 197
AflIII A'CryG_T
Cuts at: 1203 5545 6522 1203
Size: 4342 977 1327
Fragments arranged by size:
4342 1327 977
AgeI A'CCGG_T
Cuts at: 5477 5477
Size: 6646
AhdI GACnn_n'nnGTC
Cuts at: 5375 5375
Size: 6646
AleI CACnn'nnGTG
Cuts at: 1166 3542 1166
Size: 2376 4270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2315 2403 1184
Size: 1131 88 5427
Fragments arranged by size:
5427 1131 88
AlwNI CAG_nnn'CTG
Cuts at: 2927 3876 4389 5961 2927
Size: 949 513 1572 3612
Fragments arranged by size:
3612 1572 949 513
ApaLI G'TGCA_C
Cuts at: 4703 5859 4703
Size: 1156 5490
ApoI r'AATT_y
Cuts at: 1493 2465 3416 3955 4181 4333 6442 1493
Size: 972 951 539 226 152 2109 1697
Fragments arranged by size:
2109 1697 972 951 539 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5200 160
Size: 872 4168 1606
Fragments arranged by size:
4168 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 3971 1870
Size: 73 2028 4545
Fragments arranged by size:
4545 2028 73
BamHI G'GATC_C
Cuts at: 3976 4343 3976
Size: 367 6279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2343 3967 5422 618
Size: 325 217 627 556 1624 1455 1842
Fragments arranged by size:
1842 1624 1455 627 556 325 217
BanII G_rGCy'C
Cuts at: 1391 3257 3467 3704 3965 1391
Size: 1866 210 237 261 4072
Fragments arranged by size:
4072 1866 261 237 210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3867 928
Size: 971 1968 3707
Fragments arranged by size:
3707 1968 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4837 4871 1903
Size: 34 2900 34 3678
Fragments arranged by size:
3678 2900 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2389 4538 5748 2389
Size: 2149 1210 3287
Fragments arranged by size:
3287 2149 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6646
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2120 5257 136
Size: 107 122 71 631 605 448 3137 1525
Fragments arranged by size:
3137 1525 631 605 448 122 107 71
BglII A'GATC_T
Cuts at: 6641 6641
Size: 6646
BlpI GC'TnA_GC
Cuts at: 2630 4063 2630
Size: 1433 5213
Bme1580I G_kGCm'C
Cuts at: 1790 4707 5863 1790
Size: 2917 1156 2573
Fragments arranged by size:
2917 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6646
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3030 3225 4377
Size: 416 53 83 399 24 1613 195 1152
Cuts at: 4377 5335 442
Size: 958 1753
Fragments arranged by size:
1753 1613 1152 958 416 399 195 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3351 1056
Size: 2295 4351
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2469 2554 2469
Size: 85 6561
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3201 3535 3660 3696 5306 1239
Size: 1962 334 125 36 1610 2579
Fragments arranged by size:
2579 1962 1610 334 125 36
Bpu10I CC'TnA_GC
Cuts at: 3698 3698
Size: 6646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2594 4771 5636 5934 2387
Size: 207 2177 865 298 3099
Fragments arranged by size:
3099 2177 865 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5309 882
Size: 617 3810 2219
Fragments arranged by size:
3810 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3943 4437 493
Size: 711 2739 494 2702
Fragments arranged by size:
2739 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6640 6640
Size: 6646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4453 4835 5470 275
Size: 53 83 186 936 2920 382 635 1451
Fragments arranged by size:
2920 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2796 5079 5477 5751 5898 6309 2796
Size: 2283 398 274 147 411 3133
Fragments arranged by size:
3133 2283 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2400 2430 3283 3313 3781
Size: 30 165 30 997 30 853 30 468
Cuts at: 3781 3811 1178
Size: 30 4013
Fragments arranged by size:
4013 997 853 468 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2546 3271 3274 1186
Size: 1360 725 3 4558
Fragments arranged by size:
4558 1360 725 3
BseYI C'CCAG_C
Cuts at: 2319 3266 5849 6345 2319
Size: 947 2583 496 2620
Fragments arranged by size:
2620 2583 947 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2233 1163
Size: 573 497 5576
Fragments arranged by size:
5576 573 497
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4096 4857 5885 1540
Size: 275 186 2095 761 1028 2301
Fragments arranged by size:
2301 2095 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3257 3549 4707 4792 5863 3257
Size: 292 1158 85 1071 4040
Fragments arranged by size:
4040 1158 1071 292 85
BsmI GAATG_Cn'
Cuts at: 4157 4250 4157
Size: 93 6553
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3257 3467 3549 3704 3965 4707 4792
Size: 399 1467 210 82 155 261 742 85
Cuts at: 4792 5863 1391
Size: 1071 2174
Fragments arranged by size:
2174 1467 1071 742 399 261 210 155
85 82
BspEI T'CCGG_A
Cuts at: 6309 6309
Size: 6646
BspHI T'CATG_A
Cuts at: 4535 6265 4535
Size: 1730 4916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4001 844
Size: 3157 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4533 1394
Size: 429 2710 3507
Fragments arranged by size:
3507 2710 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5141 5315 65
Size: 1183 3893 174 1396
Fragments arranged by size:
3893 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3313 5290 5477 1700
Size: 110 1503 1977 187 2869
Fragments arranged by size:
2869 1977 1503 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 4706 5718 4706
Size: 1012 5634
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6646
BstBI TT'CG_AA
Cuts at: 2007 5450 2007
Size: 3443 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6646
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 3520 2029
Size: 1491 5155
BstYI r'GATC_y
Cuts at: 1972 3976 4343 4728 4745 6197 6283 6295 6641
Size: 2004 367 385 17 1452 86 12 346
Cuts at: 6641 1972
Size: 1977
Fragments arranged by size:
2004 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2989 3143 513
Size: 552 1924 154 4016
Fragments arranged by size:
4016 1924 552 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5907
Fragments arranged by size:
5907 683 56
BtsI GCAGTG_nn'
Cuts at: 2294 3273 3636 3852 4162 4956 4976 2294
Size: 979 363 216 310 794 20 3964
Fragments arranged by size:
3964 979 794 363 310 216 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6611
DraI TTT'AAA
Cuts at: 2832 3950 2832
Size: 1118 5528
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5653 809
Size: 507 4337 1802
Fragments arranged by size:
4337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3070 4093 4982
Size: 54 1425 50 275 18 1240 1023 889
Cuts at: 4982 6611 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1240 1023 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4093 1537
Size: 275 2281 4090
Fragments arranged by size:
4090 2281 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2523 2648 2673 2784 3247 3313 3886 4576
Size: 1170 125 25 111 463 66 573 690
Cuts at: 4576 1353
Size: 3423
Fragments arranged by size:
3423 1170 690 573 463 125 111 66
25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5233 5607 5753 387
Size: 1064 138 3644 374 146 1280
Fragments arranged by size:
3644 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3255 3255
Size: 6646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2424 3201 3535 3660 3696 4709
Size: 393 602 190 777 334 125 36 1013
Cuts at: 4709 5306 6093 6361 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1013 787 777 602 597 393 334
268 190 125 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3675 3675
Size: 6646
EcoO109I rG'GnC_Cy
Cuts at: 2098 2848 2098
Size: 750 5896
EcoRI G'AATT_C
Cuts at: 3955 4333 3955
Size: 378 6268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2102 2722 2102
Size: 620 6026
FspI TGC'GCA
Cuts at: 5152 5152
Size: 6646
HaeII r_GCGC'y
Cuts at: 1678 2539 5793 1678
Size: 861 3254 2531
Fragments arranged by size:
3254 2531 861
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2400 2432 3199 3231 3811
Size: 32 135 32 828 32 767 32 580
Cuts at: 3811 3843 5287 5319 5361 5393 1373
Size: 32 1444 32 42 32 2626
Fragments arranged by size:
2626 1444 828 767 580 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3990 4236 669
Size: 988 281 2052 246 3079
Fragments arranged by size:
3079 2052 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6646
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KpnI G_GTAC'C
Cuts at: 3971 3971
Size: 6646
MfeI C'AATT_G
Cuts at: 2888 4245 2888
Size: 1357 5289
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2442 5760 5944 1305
Size: 1137 3318 184 2007
Fragments arranged by size:
3318 2007 1137 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3072 10
Size: 54 1425 343 1240 3584
Fragments arranged by size:
3584 1425 1240 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 2567 2942 3542 4604 4963 5122
Size: 648 1257 144 375 600 1062 359 159
Cuts at: 5122 518
Size: 2042
Fragments arranged by size:
2042 1257 1062 648 600 375 359 159
144
MspA1I CmG'CkG
Cuts at: 1606 2323 2383 2524 2690 3520 3876 4062 4739
Size: 717 60 141 166 830 356 186 677
Cuts at: 4739 5887 6132 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 830 717 677 356 245 186
166 141 60
NcoI C'CATG_G
Cuts at: 513 1065 2989 513
Size: 552 1924 4170
Fragments arranged by size:
4170 1924 552
NdeI CA'TA_TG
Cuts at: 387 3055 387
Size: 2668 3978
NheI G'CTAG_C
Cuts at: 1052 3347 1052
Size: 2295 4351
NotI GC'GGCC_GC
Cuts at: 4093 4093
Size: 6646
NspI r_CATG'y
Cuts at: 1132 1229 2302 2314 2390 2428 4004 5549 1132
Size: 97 1073 12 76 38 1576 1545 2229
Fragments arranged by size:
2229 1576 1545 1073 97 76 38 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6646
PciI A'CATG_T
Cuts at: 5545 5545
Size: 6646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6646
PfoI T'CCnGG_A
Cuts at: 3712 3712
Size: 6646
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 6228 6260 4715
Size: 32 1481 32 5101
Fragments arranged by size:
5101 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 2848 2098
Size: 750 5896
PshAI GACnn'nnGTC
Cuts at: 1613 3218 1613
Size: 1605 5041
PsiI TTA'TAA
Cuts at: 4216 4216
Size: 6646
PstI C_TGCA'G
Cuts at: 830 3115 3859 3998 830
Size: 2285 744 139 3478
Fragments arranged by size:
3478 2285 744 139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6646
PvuII CAG'CTG
Cuts at: 2323 2524 2690 3520 3876 2323
Size: 201 166 830 356 5093
Fragments arranged by size:
5093 830 356 201 166
SacI G_AGCT'C
Cuts at: 3257 3257
Size: 6646
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SapI GCTCTTCn'nnn_
Cuts at: 2648 3313 3886 2648
Size: 665 573 5408
Fragments arranged by size:
5408 665 573
SbfI CC_TGCA'GG
Cuts at: 3998 3998
Size: 6646
ScaI AGT'ACT
Cuts at: 4894 6606 4894
Size: 1712 4934
SfcI C'TryA_G
Cuts at: 826 1045 2192 3111 3458 3855 3994 5129 5810
Size: 219 1147 919 347 397 139 1135 681
Cuts at: 5810 6001 826
Size: 191 1471
Fragments arranged by size:
1471 1147 1135 919 681 397 347 219
191 139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 3973 1872
Size: 2101 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2270 2366 2573 4750 5651 5913
Size: 197 926 327 96 207 2177 901 262
Cuts at: 5913 820
Size: 1553
Fragments arranged by size:
2177 1553 926 901 327 262 207 197
96
SnaBI TAC'GTA
Cuts at: 493 3943 4437 493
Size: 3450 494 2702
Fragments arranged by size:
3450 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6646
SphI G_CATG'C
Cuts at: 1132 2314 2390 4004 1132
Size: 1182 76 1614 3774
Fragments arranged by size:
3774 1614 1182 76
SspI AAT'ATT
Cuts at: 5 52 4570 5
Size: 47 4518 2081
Fragments arranged by size:
4518 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6646
StyI C'CwwG_G
Cuts at: 513 1065 2989 4085 513
Size: 552 1924 1096 3074
Fragments arranged by size:
3074 1924 1096 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3183 3778 3787 4673 4690 4843 5028
Size: 1255 1603 595 9 886 17 153 185
Cuts at: 5028 5473 325
Size: 445 1498
Fragments arranged by size:
1603 1498 1255 886 595 445 185 153
17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4892 6604 96
Size: 274 80 33 51 1443 2915 1712 138
Fragments arranged by size:
2915 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3628 3660 3781 3813 6606 6638 3628
Size: 32 121 32 2793 32 3636
Fragments arranged by size:
3636 2793 121 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6646
XbaI T'CTAG_A
Cuts at: 3982 3982
Size: 6646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3449 3449
Size: 6646
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6646
XmaI C'CCGG_G
Cuts at: 1870 3971 1870
Size: 2101 4545
XmnI GAAnn'nnTTC
Cuts at: 4775 4775
Size: 6646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4454 276
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI