Restriction map for
pFN21ASDA1431
(Circular) (Six-base) MAPSORT of: pFN21ASDA1431.seq Check: 7886 from: 1 to: 7333
pFN21ASDA1431 7333 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5143 278
Size: 53 83 186 936 3607 2468
Fragments arranged by size:
3607 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4676 1937
Size: 2739 4594
Acc65I G'GTAC_C
Cuts at: 4654 4654
Size: 7333
AclI AA'CG_TT
Cuts at: 1563 5460 5833 1563
Size: 3897 373 3063
Fragments arranged by size:
3897 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2997 3547 3631 3715 3883 4051 4471 5396
Size: 1365 550 84 84 168 168 420 925
Cuts at: 5396 6780 7048 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1365 925 550 420 268 168
168 84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7136
AflIII A'CryG_T
Cuts at: 1203 6232 7209 1203
Size: 5029 977 1327
Fragments arranged by size:
5029 1327 977
AgeI A'CCGG_T
Cuts at: 6164 6164
Size: 7333
AhdI GACnn_n'nnGTC
Cuts at: 6062 6062
Size: 7333
AleI CACnn'nnGTG
Cuts at: 1166 4110 1166
Size: 2944 4389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7333
AlwNI CAG_nnn'CTG
Cuts at: 5076 6648 5076
Size: 1572 5761
ApaLI G'TGCA_C
Cuts at: 5390 6546 5390
Size: 1156 6177
ApoI r'AATT_y
Cuts at: 1493 2773 3220 3342 3597 4521 4592 4642 4868
Size: 1280 447 122 255 924 71 50 226
Cuts at: 4868 5020 7129 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1280 924 447 255 226 152
122 71 50
AseI AT'TA_AT
Cuts at: 160 1032 5887 160
Size: 872 4855 1606
Fragments arranged by size:
4855 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7333
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2097 2151 4658 1870
Size: 73 125 29 54 2507 4545
Fragments arranged by size:
4545 2507 125 73 54 29
BamHI G'GATC_C
Cuts at: 4663 5030 4663
Size: 367 6966
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2538 4654 6109 618
Size: 325 217 627 291 460 2116 1455 1842
Fragments arranged by size:
2116 1842 1455 627 460 325 291 217
BanII G_rGCy'C
Cuts at: 1391 2233 2880 3320 4652 1391
Size: 842 647 440 1332 4072
Fragments arranged by size:
4072 1332 842 647 440
BbeI G_GCGC'C
Cuts at: 2082 2082
Size: 7333
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4477 928
Size: 971 2578 3784
Fragments arranged by size:
3784 2578 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5524 5558 1903
Size: 34 3587 34 3678
Fragments arranged by size:
3678 3587 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5225 6435 5225
Size: 1210 6123
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7333
BfrBI ATG'CAT
Cuts at: 3743 3743
Size: 7333
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2138 5944 136
Size: 107 122 71 631 605 466 3806 1525
Fragments arranged by size:
3806 1525 631 605 466 122 107 71
BglII A'GATC_T
Cuts at: 4454 7328 4454
Size: 2874 4459
BlpI GC'TnA_GC
Cuts at: 2838 4750 2838
Size: 1912 5421
Bme1580I G_kGCm'C
Cuts at: 1790 2226 2543 5394 6550 1790
Size: 436 317 2851 1156 2573
Fragments arranged by size:
2851 2573 1156 436 317
BmgBI CAC'GTC
Cuts at: 1318 4566 1318
Size: 3248 4085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2686 3531 3867
Size: 416 53 83 399 24 1269 845 336
Cuts at: 3867 5064 6022 442
Size: 1197 958 1753
Fragments arranged by size:
1753 1269 1197 958 845 416 399 336
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7333
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3339 3339
Size: 7333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2112 2300 2672 3372 3526 3627 3795 3963
Size: 873 188 372 700 154 101 168 168
Cuts at: 3963 4299 4467 4551 5993 1239
Size: 336 168 84 1442 2579
Fragments arranged by size:
2579 1442 873 700 372 336 188 168
168 168 154 101 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2152 2567 5458 6323 6621 2152
Size: 415 2891 865 298 2864
Fragments arranged by size:
2891 2864 865 415 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2933 5996 882
Size: 617 1434 3063 2219
Fragments arranged by size:
3063 2219 1434 617
BsaAI yAC'GTr
Cuts at: 493 1204 4630 5124 493
Size: 711 3426 494 2702
Fragments arranged by size:
3426 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7327 7327
Size: 7333
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 2079 5140 5522
Size: 53 83 186 936 527 19 3061 382
Cuts at: 5522 6157 275
Size: 635 1451
Fragments arranged by size:
3061 1451 936 635 527 382 186 83
53 19
BsaWI w'CCGG_w
Cuts at: 3689 5766 6164 6438 6585 6996 3689
Size: 2077 398 274 147 411 4026
Fragments arranged by size:
4026 2077 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2360 2390 1178
Size: 30 165 30 957 30 6121
Fragments arranged by size:
6121 957 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2214 2381 2470 1186
Size: 1028 167 89 6049
Fragments arranged by size:
6049 1028 167 89
BseYI C'CCAG_C
Cuts at: 2446 2761 6536 7032 2446
Size: 315 3775 496 2747
Fragments arranged by size:
3775 2747 496 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4172 1163
Size: 573 2436 4324
Fragments arranged by size:
4324 2436 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4783 5544 6572 1540
Size: 275 186 2782 761 1028 2301
Fragments arranged by size:
2782 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2233 3320 5394 5479 6550 2233
Size: 1087 2074 85 1071 3016
Fragments arranged by size:
3016 2074 1087 1071 85
BsmI GAATG_Cn'
Cuts at: 4058 4232 4301 4844 4937 4058
Size: 174 69 543 93 6454
Fragments arranged by size:
6454 543 174 93 69
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2233 2543 2880 3320 4652 5394
Size: 399 436 7 310 337 440 1332 742
Cuts at: 5394 5479 6550 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1332 1071 742 440 436 399 337
310 85 7
BspEI T'CCGG_A
Cuts at: 6996 6996
Size: 7333
BspHI T'CATG_A
Cuts at: 5222 6952 5222
Size: 1730 5603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4688 844
Size: 3844 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2065 2204 5220 1394
Size: 429 242 139 3016 3507
Fragments arranged by size:
3507 3016 429 242 139
BsrDI GCAATG_nn'
Cuts at: 65 1248 5828 6002 65
Size: 1183 4580 174 1396
Fragments arranged by size:
4580 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5977 6164 1700
Size: 110 4167 187 2869
Fragments arranged by size:
4167 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2403 2932 3670 4342 96
Size: 2307 529 738 672 3087
Fragments arranged by size:
3087 2307 738 672 529
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7333
BssSI C'ACGA_G
Cuts at: 5393 6405 5393
Size: 1012 6321
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4053 794
Size: 3259 4074
BstBI TT'CG_AA
Cuts at: 2007 6137 2007
Size: 4130 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7333
BstYI r'GATC_y
Cuts at: 1972 4454 4579 4663 5030 5415 5432 6884 6970
Size: 2482 125 84 367 385 17 1452 86
Cuts at: 6970 6982 7328 1972
Size: 12 346 1977
Fragments arranged by size:
2482 1977 1452 385 367 346 125 86
84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6781
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2427 4247 487
Size: 683 56 1201 1820 3573
Fragments arranged by size:
3573 1820 1201 683 56
BtsI GCAGTG_nn'
Cuts at: 2247 2625 4306 4849 5643 5663 2247
Size: 378 1681 543 794 20 3917
Fragments arranged by size:
3917 1681 794 543 378 20
ClaI AT'CG_AT
Cuts at: 1554 3546 1554
Size: 1992 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2351 2386 4557 4592 529
Size: 35 1787 35 2171 35 3270
Fragments arranged by size:
3270 2171 1787 35 35 35
DraI TTT'AAA
Cuts at: 4637 4637
Size: 7333
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6340 809
Size: 507 5024 1802
Fragments arranged by size:
5024 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4195 4780 5669
Size: 54 1425 50 275 18 2365 585 889
Cuts at: 5669 7298 8
Size: 1629 43
Fragments arranged by size:
2365 1629 1425 889 585 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4780 1537
Size: 275 2968 4090
Fragments arranged by size:
4090 2968 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 3183 5263 1353
Size: 906 924 2080 3423
Fragments arranged by size:
3423 2080 924 906
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2735 5920 6294 6440 387
Size: 1064 138 1146 3185 374 146 1280
Fragments arranged by size:
3185 1280 1146 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2231 3318 2231
Size: 1087 6246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2300 2672 2997 3372 3526 3547
Size: 393 480 188 372 325 375 154 21
Cuts at: 3547 3627 3631 3715 3795 3883 3963 4051 4299
Size: 80 4 84 80 88 80 88 248
Cuts at: 4299 4467 4471 4551 5396 5993 6780 7048 1239
Size: 168 4 80 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 597 480 393 375 372
325 268 248 188 168 154 88 88
84 80 80 80 80 21 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3128 3128
Size: 7333
EcoO109I rG'GnC_Cy
Cuts at: 2216 2216
Size: 7333
EcoRI G'AATT_C
Cuts at: 3342 3597 4521 4642 5020 3342
Size: 255 924 121 378 5655
Fragments arranged by size:
5655 924 378 255 121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 7333
FspI TGC'GCA
Cuts at: 5839 5839
Size: 7333
HaeII r_GCGC'y
Cuts at: 1678 2082 6480 1678
Size: 404 4398 2531
Fragments arranged by size:
4398 2531 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 2801 2833 2837
Size: 32 135 32 890 32 307 32 4
Cuts at: 2837 2869 2981 3013 3389 3421 3499 3531 4148
Size: 32 112 32 376 32 78 32 617
Cuts at: 4148 4180 5974 6006 6048 6080 1373
Size: 32 1794 32 42 32 2626
Fragments arranged by size:
2626 1794 890 617 376 307 135 112
78 42 32 32 32 32 32 32
32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2740 4542 4677 4923 669
Size: 988 281 802 1802 135 246 3079
Fragments arranged by size:
3079 1802 988 802 281 246 135
HindIII A'AGCT_T
Cuts at: 748 3065 3275 3471 3810 748
Size: 2317 210 196 339 4271
Fragments arranged by size:
4271 2317 339 210 196
HpaI GTT'AAC
Cuts at: 4923 4923
Size: 7333
KasI G'GCGC_C
Cuts at: 2078 2078
Size: 7333
KpnI G_GTAC'C
Cuts at: 4658 4658
Size: 7333
MfeI C'AATT_G
Cuts at: 4932 4932
Size: 7333
MluI A'CGCG_T
Cuts at: 7209 7209
Size: 7333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2400 2466 6447 6631 1305
Size: 1095 66 3981 184 2007
Fragments arranged by size:
3981 2007 1095 184 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4197 10
Size: 54 1425 343 2365 3146
Fragments arranged by size:
3146 2365 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3204 3706 4042 4110 4378 5291 5650
Size: 648 2038 502 336 68 268 913 359
Cuts at: 5650 5809 518
Size: 159 2042
Fragments arranged by size:
2042 2038 913 648 502 359 336 268
159 68
MspA1I CmG'CkG
Cuts at: 1606 4749 5426 6574 6819 1606
Size: 3143 677 1148 245 2120
Fragments arranged by size:
3143 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2079 2079
Size: 7333
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6781
NdeI CA'TA_TG
Cuts at: 387 2971 3703 4039 4375 387
Size: 2584 732 336 336 3345
Fragments arranged by size:
3345 2584 732 336 336
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7333
NotI GC'GGCC_GC
Cuts at: 4780 4780
Size: 7333
NsiI A_TGCA'T
Cuts at: 3745 3745
Size: 7333
NspI r_CATG'y
Cuts at: 1132 1229 4548 4691 6236 1132
Size: 97 3319 143 1545 2229
Fragments arranged by size:
3319 2229 1545 143 97
PasI CC'CwG_GG
Cuts at: 1348 2320 1348
Size: 972 6361
PciI A'CATG_T
Cuts at: 6232 6232
Size: 7333
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2326 1108
Size: 1218 6115
PmeI GTTT'AAAC
Cuts at: 4637 4637
Size: 7333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5402 5434 6915 6947 5402
Size: 32 1481 32 5788
Fragments arranged by size:
5788 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7333
PsiI TTA'TAA
Cuts at: 3366 4461 4903 3366
Size: 1095 442 5796
Fragments arranged by size:
5796 1095 442
PstI C_TGCA'G
Cuts at: 830 4685 830
Size: 3855 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7333
SacI G_AGCT'C
Cuts at: 2233 3320 2233
Size: 1087 6246
SalI G'TCGA_C
Cuts at: 1936 4675 1936
Size: 2739 4594
SapI GCTCTTCn'nnn_
Cuts at: 2259 2259
Size: 7333
SbfI CC_TGCA'GG
Cuts at: 4685 4685
Size: 7333
ScaI AGT'ACT
Cuts at: 2657 5581 7293 2657
Size: 2924 1712 2697
Fragments arranged by size:
2924 2697 1712
SfcI C'TryA_G
Cuts at: 826 1045 2118 2349 2428 4681 5816 6497 6688
Size: 219 1073 231 79 2253 1135 681 191
Cuts at: 6688 826
Size: 1471
Fragments arranged by size:
2253 1471 1135 1073 681 231 219 191
79
SfoI GGC'GCC
Cuts at: 2080 2080
Size: 7333
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7333
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7333
SmaI CCC'GGG
Cuts at: 1872 2070 2099 2153 4660 1872
Size: 198 29 54 2507 4545
Fragments arranged by size:
4545 2507 198 54 29
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2167 2582 5437 6338 6600 820
Size: 197 926 224 415 2855 901 262 1553
Fragments arranged by size:
2855 1553 926 901 415 262 224 197
SnaBI TAC'GTA
Cuts at: 493 4630 5124 493
Size: 4137 494 2702
Fragments arranged by size:
4137 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7333
SphI G_CATG'C
Cuts at: 1132 4691 1132
Size: 3559 3774
SspI AAT'ATT
Cuts at: 5 52 5257 5
Size: 47 5205 2081
Fragments arranged by size:
5205 2081 47
StuI AGG'CCT
Cuts at: 1517 3389 4148 1517
Size: 1872 759 4702
Fragments arranged by size:
4702 1872 759
StyI C'CwwG_G
Cuts at: 513 1065 2136 2504 4772 513
Size: 552 1071 368 2268 3074
Fragments arranged by size:
3074 2268 1071 552 368
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2775 4001 5360 5377 5530 5715 6160
Size: 1255 1195 1226 1359 17 153 185 445
Cuts at: 6160 325
Size: 1498
Fragments arranged by size:
1498 1359 1255 1226 1195 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2403 2655 2932
Size: 274 80 33 51 1443 426 252 277
Cuts at: 2932 3670 3766 4169 4342 5579 7291 96
Size: 738 96 403 173 1237 1712 138
Fragments arranged by size:
1712 1443 1237 738 426 403 277 274
252 173 138 96 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2239 2271 2360 2392 2817 2849 4555 4587 7293
Size: 32 89 32 425 32 1706 32 2706
Cuts at: 7293 7325 2239
Size: 32 2247
Fragments arranged by size:
2706 2247 1706 425 89 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7333
XbaI T'CTAG_A
Cuts at: 4669 4669
Size: 7333
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7333
XmaI C'CCGG_G
Cuts at: 1870 2068 2097 2151 4658 1870
Size: 198 29 54 2507 4545
Fragments arranged by size:
4545 2507 198 54 29
XmnI GAAnn'nnTTC
Cuts at: 2591 2774 2952 3473 3560 4357 4400 5462 2591
Size: 183 178 521 87 797 43 1062 4462
Fragments arranged by size:
4462 1062 797 521 183 178 87 43
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5141 276
Size: 53 83 186 936 3607 2468
Fragments arranged by size:
3607 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SrfI SwaI XcmI