Restriction map for
pFN21ASDA1427
(Circular) (Six-base) MAPSORT of: pFN21ASDA1427.seq Check: 3698 from: 1 to: 6007
pFN21ASDA1427 6007 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2111 2362 2111
Size: 251 5756
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2333 3350 1937
Size: 396 1017 4594
Fragments arranged by size:
4594 1017 396
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2844 2873 3062 4070 5454 5722 1632
Size: 1212 29 189 1008 1384 268 1917
Fragments arranged by size:
1917 1384 1212 1008 268 189 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 4906 5883 1203
Size: 3703 977 1327
Fragments arranged by size:
3703 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 4736 4736
Size: 6007
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3201 1184
Size: 2017 3990
AlwNI CAG_nnn'CTG
Cuts at: 2885 3240 3750 5322 2885
Size: 355 510 1572 3570
Fragments arranged by size:
3570 1572 510 355
ApaLI G'TGCA_C
Cuts at: 4064 5220 4064
Size: 1156 4851
ApoI r'AATT_y
Cuts at: 1493 3316 3542 3694 5803 1493
Size: 1823 226 152 2109 1697
Fragments arranged by size:
2109 1823 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 2189 3220 3332 1870
Size: 73 246 1031 112 4545
Fragments arranged by size:
4545 1031 246 112 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2054 3285 3328 4783
Size: 325 217 627 248 19 1231 43 1455
Cuts at: 4783 618
Size: 1842
Fragments arranged by size:
1842 1455 1231 627 325 248 217 43
19
BanII G_rGCy'C
Cuts at: 1391 2346 2724 2796 2988 3227 3326 1391
Size: 955 378 72 192 239 99 4072
Fragments arranged by size:
4072 955 378 239 192 99 72
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 6007
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2861 928
Size: 971 962 4074
Fragments arranged by size:
4074 971 962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3014 3048 4198 4232 1903
Size: 34 1077 34 1150 34 3678
Fragments arranged by size:
3678 1150 1077 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 3099 1548
Size: 1551 4456
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6002 6002
Size: 6007
BlpI GC'TnA_GC
Cuts at: 3066 3424 3066
Size: 358 5649
Bme1580I G_kGCm'C
Cuts at: 1790 2069 2248 4068 5224 1790
Size: 279 179 1820 1156 2573
Fragments arranged by size:
2573 1820 1156 279 179
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2833 3250 3738
Size: 416 53 83 399 24 1416 417 488
Cuts at: 3738 4696 442
Size: 958 1753
Fragments arranged by size:
1753 1416 958 488 417 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2280 2907 3121 4667 1239
Size: 947 94 627 214 1546 2579
Fragments arranged by size:
2579 1546 947 627 214 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2372 2770 4132 4997 5295 2237
Size: 135 398 1362 865 298 2949
Fragments arranged by size:
2949 1362 865 398 298 135
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2453 4670 882
Size: 617 954 2217 2219
Fragments arranged by size:
2219 2217 954 617
BsaAI yAC'GTr
Cuts at: 493 1204 3304 3798 493
Size: 711 2100 494 2702
Fragments arranged by size:
2702 2100 711 494
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3814 4196 4831
Size: 53 83 186 936 522 1759 382 635
Cuts at: 4831 275
Size: 1451
Fragments arranged by size:
1759 1451 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2753 2783 1178
Size: 30 165 30 1350 30 4402
Fragments arranged by size:
4402 1350 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 2354 2420 2702 2729 2732 3260 1186
Size: 1054 114 66 282 27 3 528 3933
Fragments arranged by size:
3933 1054 528 282 114 66 27 3
BseYI C'CCAG_C
Cuts at: 2050 2062 2180 3157 5210 5706 2050
Size: 12 118 977 2053 496 2351
Fragments arranged by size:
2351 2053 977 496 118 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2181 2334 2657 3197 3278 1163
Size: 573 445 153 323 540 81 3892
Fragments arranged by size:
3892 573 540 445 323 153 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2764 3457 4218 5246 1540
Size: 275 186 763 693 761 1028 2301
Fragments arranged by size:
2301 1028 763 761 693 275 186
BsiHKAI G_wGCw'C
Cuts at: 2703 2724 2796 2988 4068 4153 5224 2703
Size: 21 72 192 1080 85 1071 3486
Fragments arranged by size:
3486 1080 1071 192 85 72 21
BsmI GAATG_Cn'
Cuts at: 3518 3611 3518
Size: 93 5914
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2248 2346 2703 2724 2796 2988
Size: 399 279 179 98 357 21 72 192
Cuts at: 2988 3227 3326 4068 4153 5224 1391
Size: 239 99 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 357 279 239 192
179 99 98 85 72 21
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 3896 5626 3896
Size: 1730 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2111 2362 2386 2765 3132 3362 844
Size: 1267 251 24 379 367 230 3489
Fragments arranged by size:
3489 1267 379 367 251 230 24
BsrBI CCG'CTC
Cuts at: 1394 1823 3894 1394
Size: 429 2071 3507
Fragments arranged by size:
3507 2071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2630 3267 4651 4838 1700
Size: 110 820 637 1384 187 2869
Fragments arranged by size:
2869 1384 820 637 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 4067 5079 4067
Size: 1012 4995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3292 794
Size: 2498 3509
BstBI TT'CG_AA
Cuts at: 2007 4811 2007
Size: 2804 3203
BstEII G'GTnAC_C
Cuts at: 1184 2098 3013 1184
Size: 914 915 4178
Fragments arranged by size:
4178 915 914
BstYI r'GATC_y
Cuts at: 1972 3337 3704 4089 4106 5558 5644 5656 6002
Size: 1365 367 385 17 1452 86 12 346
Cuts at: 6002 1972
Size: 1977
Fragments arranged by size:
1977 1452 1365 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2144 2223 513
Size: 552 957 122 79 4297
Fragments arranged by size:
4297 957 552 122 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 2346 3196 3523 4317 4337 2346
Size: 850 327 794 20 4016
Fragments arranged by size:
4016 850 794 327 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DraIII CAC_nnn'GTG
Cuts at: 2782 2782
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2603 5014 809
Size: 507 1287 2411 1802
Fragments arranged by size:
2411 1802 1287 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2619 2784 3193
Size: 54 1425 50 275 18 789 165 409
Cuts at: 3193 3454 4343 5972 8
Size: 261 889 1629 43
Fragments arranged by size:
1629 1425 889 789 409 275 261 165
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3937 1353
Size: 2584 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2726 2786 4594 4968 5114 387
Size: 1064 138 1137 60 1808 374 146 1280
Fragments arranged by size:
1808 1280 1137 1064 374 146 138 60
EcoICRI GAG'CTC
Cuts at: 2722 2794 2986 2722
Size: 72 192 5743
Fragments arranged by size:
5743 192 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2280 2844 2873 2907 3062 3121
Size: 393 554 94 564 29 34 155 59
Cuts at: 3121 4070 4667 5454 5722 1239
Size: 949 597 787 268 1524
Fragments arranged by size:
1524 949 787 597 564 554 393 268
155 94 59 34 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2168 2711 2993 3128 2168
Size: 543 282 135 5047
Fragments arranged by size:
5047 543 282 135
EcoO109I rG'GnC_Cy
Cuts at: 2155 2497 2692 2754 2893 2155
Size: 342 195 62 139 5269
Fragments arranged by size:
5269 342 195 139 62
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 3034 2200
Size: 834 5173
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 2058 5154 1678
Size: 380 3096 2531
Fragments arranged by size:
3096 2531 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2753 2785 2801 2833 4648
Size: 32 135 32 1181 32 16 32 1815
Cuts at: 4648 4680 4722 4754 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1815 1181 135 42 32 32 32
32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3351 3597 669
Size: 988 281 1413 246 3079
Fragments arranged by size:
3079 1413 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 6007
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2468 5121 5305 1305
Size: 1163 2653 184 2007
Fragments arranged by size:
2653 2007 1163 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2621 3195 10
Size: 54 1425 343 789 574 2822
Fragments arranged by size:
2822 1425 789 574 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3965 4324 4483 518
Size: 648 2799 359 159 2042
Fragments arranged by size:
2799 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2285 2671 3292 3423 4100 5248 5493
Size: 540 139 386 621 131 677 1148 245
Cuts at: 5493 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 621 540 386 245 139
131
NaeI GCC'GGC
Cuts at: 3269 3269
Size: 6007
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 6007
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4498
Fragments arranged by size:
4498 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NgoMIV G'CCGG_C
Cuts at: 3267 3267
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 2122 3365 4910 1132
Size: 97 893 1243 1545 2229
Fragments arranged by size:
2229 1545 1243 893 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6007
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2396 2428 2951 2983 4076 4108 5589 5621 2396
Size: 32 523 32 1093 32 1481 32 2782
Fragments arranged by size:
2782 1481 1093 523 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2754 2893 2155
Size: 599 139 5269
Fragments arranged by size:
5269 599 139
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PstI C_TGCA'G
Cuts at: 830 2881 3132 3203 3359 830
Size: 2051 251 71 156 3478
Fragments arranged by size:
3478 2051 251 156 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2285 2671 3292 2285
Size: 386 621 5000
Fragments arranged by size:
5000 621 386
SacI G_AGCT'C
Cuts at: 2724 2796 2988 2724
Size: 72 192 5743
Fragments arranged by size:
5743 192 72
SacII CC_GC'GG
Cuts at: 2147 2147
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SfcI C'TryA_G
Cuts at: 826 1045 2877 3128 3199 3355 4490 5171 5362
Size: 219 1832 251 71 156 1135 681 191
Cuts at: 5362 826
Size: 1471
Fragments arranged by size:
1832 1471 1135 681 251 219 191 156
71
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 6007
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 2387 2749 4111 5012 5274
Size: 197 926 309 135 362 1362 901 262
Cuts at: 5274 820
Size: 1553
Fragments arranged by size:
1553 1362 926 901 362 309 262 197
135
SnaBI TAC'GTA
Cuts at: 493 3304 3798 493
Size: 2811 494 2702
Fragments arranged by size:
2811 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 3134 1517
Size: 1617 4390
StyI C'CwwG_G
Cuts at: 513 1065 2022 2608 2979 3446 513
Size: 552 957 586 371 467 3074
Fragments arranged by size:
3074 957 586 552 467 371
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4034 4051 4204 4389 4834 325
Size: 1255 2454 17 153 185 445 1498
Fragments arranged by size:
2454 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4253 5965 96
Size: 274 80 33 51 1443 2276 1712 138
Fragments arranged by size:
2276 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5967 5999 5967
Size: 32 5975
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6007
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2932 2986 3145 2932
Size: 54 159 5794
Fragments arranged by size:
5794 159 54
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 2232 4136 2232
Size: 1904 4103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI
SwaI