Restriction map for
pFN21ASDA1412
(Circular) (Six-base) MAPSORT of: pFN21ASDA1412.seq Check: 316 from: 1 to: 8878
pFN21ASDA1412 8878 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5608 5608
Size: 8878
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6688 278
Size: 53 83 186 936 5152 2468
Fragments arranged by size:
5152 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3459 6221 1937
Size: 1522 2762 4594
Fragments arranged by size:
4594 2762 1522
Acc65I G'GTAC_C
Cuts at: 6199 6199
Size: 8878
AclI AA'CG_TT
Cuts at: 1563 7005 7378 1563
Size: 5442 373 3063
Fragments arranged by size:
5442 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2142 2235 2897 3588 6941 8325 8593 1632
Size: 510 93 662 691 3353 1384 268 1917
Fragments arranged by size:
3353 1917 1384 691 662 510 268 93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8681
AflIII A'CryG_T
Cuts at: 1203 4327 4789 7777 8754 1203
Size: 3124 462 2988 977 1327
Fragments arranged by size:
3124 2988 1327 977 462
AgeI A'CCGG_T
Cuts at: 7709 7709
Size: 8878
AhdI GACnn_n'nnGTC
Cuts at: 3736 7607 3736
Size: 3871 5007
AleI CACnn'nnGTG
Cuts at: 1166 3438 3681 1166
Size: 2272 243 6363
Fragments arranged by size:
6363 2272 243
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2617 3788 5002 1184
Size: 1433 1171 1214 5060
Fragments arranged by size:
5060 1433 1214 1171
AlwNI CAG_nnn'CTG
Cuts at: 3430 5451 5517 6621 8193 3430
Size: 2021 66 1104 1572 4115
Fragments arranged by size:
4115 2021 1572 1104 66
ApaI G_GGCC'C
Cuts at: 4653 4653
Size: 8878
ApaLI G'TGCA_C
Cuts at: 6935 8091 6935
Size: 1156 7722
ApoI r'AATT_y
Cuts at: 1493 2165 2764 2856 3167 3277 3480 3595 4192
Size: 672 599 92 311 110 203 115 597
Cuts at: 4192 4414 4735 5375 6187 6413 6565 8674 1493
Size: 222 321 640 812 226 152 2109 1697
Fragments arranged by size:
2109 1697 812 672 640 599 597 321
311 226 222 203 152 115 110 92
AseI AT'TA_AT
Cuts at: 160 1032 4047 5097 7432 160
Size: 872 3015 1050 2335 1606
Fragments arranged by size:
3015 2335 1606 1050 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8878
AvaI C'yCGr_G
Cuts at: 1870 1943 2814 6203 1870
Size: 73 871 3389 4545
Fragments arranged by size:
4545 3389 871 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4696 4729 4696
Size: 33 8845
BamHI G'GATC_C
Cuts at: 2392 2525 2935 5773 6208 6575 2392
Size: 133 410 2838 435 367 4695
Fragments arranged by size:
4695 2838 435 410 367 133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5176 6199 7654 618
Size: 325 217 627 3389 1023 1455 1842
Fragments arranged by size:
3389 1842 1455 1023 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4653 6197 1391
Size: 3262 1544 4072
Fragments arranged by size:
4072 3262 1544
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2193 928
Size: 971 294 7613
Fragments arranged by size:
7613 971 294
BbvCI CC'TCA_GC
Cuts at: 3471 3471
Size: 8878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7069 7103 1903
Size: 34 5132 34 3678
Fragments arranged by size:
5132 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3701 4892 5076 6770 7980 3701
Size: 1191 184 1694 1210 4599
Fragments arranged by size:
4599 1694 1210 1191 184
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7489 136
Size: 107 122 71 631 605 5817 1525
Fragments arranged by size:
5817 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8873 8873
Size: 8878
BlpI GC'TnA_GC
Cuts at: 6295 6295
Size: 8878
Bme1580I G_kGCm'C
Cuts at: 1790 2728 4653 6939 8095 1790
Size: 938 1925 2286 1156 2573
Fragments arranged by size:
2573 2286 1925 1156 938
BmgBI CAC'GTC
Cuts at: 1318 3691 1318
Size: 2373 6505
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3056 4119 6609
Size: 416 53 83 399 24 1639 1063 2490
Cuts at: 6609 7567 442
Size: 958 1753
Fragments arranged by size:
2490 1753 1639 1063 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8878
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2459 2459
Size: 8878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2331 2732 3330 4593 4680 5162 5374 7538
Size: 1092 401 598 1263 87 482 212 2164
Cuts at: 7538 1239
Size: 2579
Fragments arranged by size:
2579 2164 1263 1092 598 482 401 212
87
Bpu10I CC'TnA_GC
Cuts at: 3471 3471
Size: 8878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3742 7003 7868 8166 3742
Size: 3261 865 298 4454
Fragments arranged by size:
4454 3261 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3306 5694 6129 7541 882
Size: 617 1807 2388 435 1412 2219
Fragments arranged by size:
2388 2219 1807 1412 617 435
BsaAI yAC'GTr
Cuts at: 493 1204 4328 6175 6669 493
Size: 711 3124 1847 494 2702
Fragments arranged by size:
3124 2702 1847 711 494
BsaBI GATnn'nnATC
Cuts at: 2370 5064 8872 2370
Size: 2694 3808 2376
Fragments arranged by size:
3808 2694 2376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2460 5754 6685 7067
Size: 53 83 186 936 927 3294 931 382
Cuts at: 7067 7702 275
Size: 635 1451
Fragments arranged by size:
3294 1451 936 931 927 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 7311 7709 7983 8130 8541 7311
Size: 398 274 147 411 7648
Fragments arranged by size:
7648 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4371 4401 1178
Size: 30 165 30 2968 30 5655
Fragments arranged by size:
5655 2968 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2127 2130 3374 3881 4407 4410 5750 5913
Size: 941 3 1244 507 526 3 1340 163
Cuts at: 5913 1186
Size: 4151
Fragments arranged by size:
4151 1340 1244 941 526 507 163 3
3
BseYI C'CCAG_C
Cuts at: 5691 8081 8577 5691
Size: 2390 496 5992
Fragments arranged by size:
5992 2390 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3416 5769 5800 1163
Size: 573 1680 2353 31 4241
Fragments arranged by size:
4241 2353 1680 573 31
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6328 7089 8117 1540
Size: 275 186 4327 761 1028 2301
Fragments arranged by size:
4327 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4673 5030 5088 5894 6939 7024 8095 4673
Size: 357 58 806 1045 85 1071 5456
Fragments arranged by size:
5456 1071 1045 806 357 85 58
BsmI GAATG_Cn'
Cuts at: 2204 2470 3392 4981 5335 6389 6482 2204
Size: 266 922 1589 354 1054 93 4600
Fragments arranged by size:
4600 1589 1054 922 354 266 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2384 2384
Size: 8878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2728 4653 4673 5030 5088 5894 6197
Size: 399 938 1925 20 357 58 806 303
Cuts at: 6197 6939 7024 8095 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1925 1071 938 806 742 399 357
303 85 58 20
BspEI T'CCGG_A
Cuts at: 8541 8541
Size: 8878
BspHI T'CATG_A
Cuts at: 4728 6767 8497 4728
Size: 2039 1730 5109
Fragments arranged by size:
5109 2039 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5608 6233 844
Size: 4764 625 3489
Fragments arranged by size:
4764 3489 625
BsrBI CCG'CTC
Cuts at: 1394 1823 6765 1394
Size: 429 4942 3507
Fragments arranged by size:
4942 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2123 2902 3112 4353 7373 7547 65
Size: 1183 875 779 210 1241 3020 174 1396
Fragments arranged by size:
3020 1396 1241 1183 875 779 210 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5173 5575 7522 7709 1700
Size: 110 3363 402 1947 187 2869
Fragments arranged by size:
3363 2869 1947 402 187 110
BsrGI T'GTAC_A
Cuts at: 96 5014 96
Size: 4918 3960
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8878
BssSI C'ACGA_G
Cuts at: 4772 5055 6938 7950 4772
Size: 283 1883 1012 5700
Fragments arranged by size:
5700 1883 1012 283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3102 794
Size: 2308 6570
BstBI TT'CG_AA
Cuts at: 2007 7682 2007
Size: 5675 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8878
BstXI CCAn_nnnn'nTGG
Cuts at: 2573 2860 5080 5168 5438 5992 2573
Size: 287 2220 88 270 554 5459
Fragments arranged by size:
5459 2220 554 287 270 88
BstYI r'GATC_y
Cuts at: 1972 2392 2525 2935 5481 5773 6208 6575 6960
Size: 420 133 410 2546 292 435 367 385
Cuts at: 6960 6977 8429 8515 8527 8873 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2546 1977 1452 435 420 410 385 367
346 292 133 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2770 2770
Size: 8878
BtgI C'CryG_G
Cuts at: 513 1065 5180 513
Size: 552 4115 4211
Fragments arranged by size:
4211 4115 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8139
Fragments arranged by size:
8139 683 56
BtsI GCAGTG_nn'
Cuts at: 3145 4717 4986 4996 6394 7188 7208 3145
Size: 1572 269 10 1398 794 20 4815
Fragments arranged by size:
4815 1572 1398 794 269 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3708 3743 529
Size: 35 3144 35 5664
Fragments arranged by size:
5664 3144 35 35
DraI TTT'AAA
Cuts at: 4363 5853 6013 6182 4363
Size: 1490 160 169 7059
Fragments arranged by size:
7059 1490 169 160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4223 7885 809
Size: 507 2907 3662 1802
Fragments arranged by size:
3662 2907 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2850 3184 5440
Size: 54 1425 50 275 18 1020 334 2256
Cuts at: 5440 5523 5577 6325 7214 8843 8
Size: 83 54 748 889 1629 43
Fragments arranged by size:
2256 1629 1425 1020 889 748 334 275
83 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6325 1537
Size: 275 4513 4090
Fragments arranged by size:
4513 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3375 5838 6127 6808 1353
Size: 2022 2463 289 681 3423
Fragments arranged by size:
3423 2463 2022 681 289
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4931 7465 7839 7985 387
Size: 1064 138 3342 2534 374 146 1280
Fragments arranged by size:
3342 2534 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2235 2331 2732 2897 3330 3588
Size: 393 510 93 96 401 165 433 258
Cuts at: 3588 4593 4680 5162 5374 6941 7538 8325 8593
Size: 1005 87 482 212 1567 597 787 268
Cuts at: 8593 1239
Size: 1524
Fragments arranged by size:
1567 1524 1005 787 597 510 482 433
401 393 268 258 212 165 96 93
87
EcoNI CCTnn'n_nnAGG
Cuts at: 2564 2564
Size: 8878
EcoO109I rG'GnC_Cy
Cuts at: 2757 2787 2818 4995 6055 2757
Size: 30 31 2177 1060 5580
Fragments arranged by size:
5580 2177 1060 31 30
EcoRI G'AATT_C
Cuts at: 2764 3480 4735 6187 6565 2764
Size: 716 1255 1452 378 5077
Fragments arranged by size:
5077 1452 1255 716 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3117 4222 4689 5128 3117
Size: 1105 467 439 6867
Fragments arranged by size:
6867 1105 467 439
FspI TGC'GCA
Cuts at: 7384 7384
Size: 8878
HaeII r_GCGC'y
Cuts at: 1678 2582 8025 1678
Size: 904 5443 2531
Fragments arranged by size:
5443 2531 904
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2224 2256 3722 3754 4012
Size: 32 135 32 652 32 1466 32 258
Cuts at: 4012 4044 4116 4148 4371 4389 4403 4421 4847
Size: 32 72 32 223 18 14 18 426
Cuts at: 4847 4879 4930 4962 5054 5086 5466 5498 7519
Size: 32 51 32 92 32 380 32 2021
Cuts at: 7519 7551 7593 7625 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2021 1466 652 426 380 258 223
135 92 72 51 42 32 32 32
32 32 32 32 32 32 32 32
32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 5206 6222 6468 669
Size: 988 281 3268 1016 246 3079
Fragments arranged by size:
3268 3079 1016 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2154 3060 4465 6156 748
Size: 1406 906 1405 1691 3470
Fragments arranged by size:
3470 1691 1406 1405 906
HpaI GTT'AAC
Cuts at: 6468 6468
Size: 8878
KpnI G_GTAC'C
Cuts at: 6203 6203
Size: 8878
MfeI C'AATT_G
Cuts at: 4716 6477 4716
Size: 1761 7117
MluI A'CGCG_T
Cuts at: 8754 8754
Size: 8878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2089 2924 3281 3457 5097 7992 8176 1305
Size: 784 835 357 176 1640 2895 184 2007
Fragments arranged by size:
2895 2007 1640 835 784 357 184 176
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3186 5442 5525 10
Size: 54 1425 343 1354 2256 83 3363
Fragments arranged by size:
3363 2256 1425 1354 343 83 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3005 3138 3438 3681 3770 3980 4305
Size: 648 1839 133 300 243 89 210 325
Cuts at: 4305 5166 5436 5967 6836 7195 7354 518
Size: 861 270 531 869 359 159 2042
Fragments arranged by size:
2042 1839 869 861 648 531 359 325
300 270 243 210 159 133 89
MspA1I CmG'CkG
Cuts at: 1606 4058 5290 6091 6294 6971 8119 8364 1606
Size: 2452 1232 801 203 677 1148 245 2120
Fragments arranged by size:
2452 2120 1232 1148 801 677 245 203
NaeI GCC'GGC
Cuts at: 5175 5577 5175
Size: 402 8476
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8326
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8878
NgoMIV G'CCGG_C
Cuts at: 5173 5575 5173
Size: 402 8476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8878
NotI GC'GGCC_GC
Cuts at: 6325 6325
Size: 8878
NspI r_CATG'y
Cuts at: 1132 1229 3719 4793 6236 7781 1132
Size: 97 2490 1074 1443 1545 2229
Fragments arranged by size:
2490 2229 1545 1443 1074 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8878
PciI A'CATG_T
Cuts at: 4789 7777 4789
Size: 2988 5890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2672 3046 3322 3883 5163 1108
Size: 1564 374 276 561 1280 4823
Fragments arranged by size:
4823 1564 1280 561 374 276
PfoI T'CCnGG_A
Cuts at: 2395 2565 2931 3012 2395
Size: 170 366 81 8261
Fragments arranged by size:
8261 366 170 81
PmeI GTTT'AAAC
Cuts at: 6182 6182
Size: 8878
PmlI CAC'GTG
Cuts at: 4328 4328
Size: 8878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6947 6979 8460 8492 6947
Size: 32 1481 32 7333
Fragments arranged by size:
7333 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2757 2787 4995 2757
Size: 30 2208 6640
Fragments arranged by size:
6640 2208 30
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8878
PsiI TTA'TAA
Cuts at: 3699 4173 6448 3699
Size: 474 2275 6129
Fragments arranged by size:
6129 2275 474
PspOMI G'GGCC_C
Cuts at: 4649 4649
Size: 8878
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6063 6095 6063
Size: 32 8846
PstI C_TGCA'G
Cuts at: 830 4063 4991 6230 830
Size: 3233 928 1239 3478
Fragments arranged by size:
3478 3233 1239 928
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8878
PvuII CAG'CTG
Cuts at: 4058 6091 4058
Size: 2033 6845
SalI G'TCGA_C
Cuts at: 1936 6220 1936
Size: 4284 4594
SbfI CC_TGCA'GG
Cuts at: 6230 6230
Size: 8878
ScaI AGT'ACT
Cuts at: 5543 7126 8838 5543
Size: 1583 1712 5583
Fragments arranged by size:
5583 1712 1583
SfcI C'TryA_G
Cuts at: 826 1045 2475 3052 4059 4987 5545 6226 7361
Size: 219 1430 577 1007 928 558 681 1135
Cuts at: 7361 8042 8233 826
Size: 681 191 1471
Fragments arranged by size:
1471 1430 1135 1007 928 681 681 577
558 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8878
SmaI CCC'GGG
Cuts at: 1872 2816 6205 1872
Size: 944 3389 4545
Fragments arranged by size:
4545 3389 944
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3757 6982 7883 8145 820
Size: 197 926 1814 3225 901 262 1553
Fragments arranged by size:
3225 1814 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6175 6669 493
Size: 5682 494 2702
Fragments arranged by size:
5682 2702 494
SpeI A'CTAG_T
Cuts at: 152 2288 5630 5932 152
Size: 2136 3342 302 3098
Fragments arranged by size:
3342 3098 2136 302
SphI G_CATG'C
Cuts at: 1132 6236 1132
Size: 5104 3774
SspI AAT'ATT
Cuts at: 5 52 2536 3496 6802 5
Size: 47 2484 960 3306 2081
Fragments arranged by size:
3306 2484 2081 960 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8878
StyI C'CwwG_G
Cuts at: 513 1065 3040 3505 4256 5870 6058 6317 513
Size: 552 1975 465 751 1614 188 259 3074
Fragments arranged by size:
3074 1975 1614 751 552 465 259 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2106 3428 6905 6922 7075 7260 7705
Size: 1255 526 1322 3477 17 153 185 445
Cuts at: 7705 325
Size: 1498
Fragments arranged by size:
3477 1498 1322 1255 526 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5014 5541 7124
Size: 274 80 33 51 1443 3037 527 1583
Cuts at: 7124 8836 96
Size: 1712 138
Fragments arranged by size:
3037 1712 1583 1443 527 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3358 3390 8838 8870 3358
Size: 32 5448 32 3366
Fragments arranged by size:
5448 3366 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5595 1316
Size: 4279 4599
XbaI T'CTAG_A
Cuts at: 6214 6214
Size: 8878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2072 4880 2072
Size: 2808 6070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8878
XmaI C'CCGG_G
Cuts at: 1870 2814 6203 1870
Size: 944 3389 4545
Fragments arranged by size:
4545 3389 944
XmnI GAAnn'nnTTC
Cuts at: 3097 7007 3097
Size: 3910 4968
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6686 276
Size: 53 83 186 936 5152 2468
Fragments arranged by size:
5152 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI