Restriction map for
pFN21ASDA1400
(Circular) (Six-base) MAPSORT of: pFN21ASDA1400.seq Check: 5245 from: 1 to: 7849
pFN21ASDA1400 7849 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3187 3187
Size: 7849
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5659 278
Size: 53 83 186 936 4123 2468
Fragments arranged by size:
4123 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2784 3423 5192 1937
Size: 847 639 1769 4594
Fragments arranged by size:
4594 1769 847 639
Acc65I G'GTAC_C
Cuts at: 5170 5170
Size: 7849
AclI AA'CG_TT
Cuts at: 1563 5976 6349 1563
Size: 4413 373 3063
Fragments arranged by size:
4413 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2157 2872 3085 3622 3650 5912 7296 7564
Size: 525 715 213 537 28 2262 1384 268
Cuts at: 7564 1632
Size: 1917
Fragments arranged by size:
2262 1917 1384 715 537 525 268 213
28
AflII C'TTAA_G
Cuts at: 820 1017 4532 820
Size: 197 3515 4137
Fragments arranged by size:
4137 3515 197
AflIII A'CryG_T
Cuts at: 1203 3429 6748 7725 1203
Size: 2226 3319 977 1327
Fragments arranged by size:
3319 2226 1327 977
AgeI A'CCGG_T
Cuts at: 4595 6680 4595
Size: 2085 5764
AhdI GACnn_n'nnGTC
Cuts at: 6578 6578
Size: 7849
AleI CACnn'nnGTG
Cuts at: 1166 2120 2825 3149 3827 4043 4976 1166
Size: 954 705 324 678 216 933 4039
Fragments arranged by size:
4039 954 933 705 678 324 216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4955 1184
Size: 3771 4078
AlwNI CAG_nnn'CTG
Cuts at: 3682 4973 5592 7164 3682
Size: 1291 619 1572 4367
Fragments arranged by size:
4367 1572 1291 619
ApaI G_GGCC'C
Cuts at: 3026 3026
Size: 7849
ApaLI G'TGCA_C
Cuts at: 2609 5906 7062 2609
Size: 3297 1156 3396
Fragments arranged by size:
3396 3297 1156
ApoI r'AATT_y
Cuts at: 1493 5158 5384 5536 7645 1493
Size: 3665 226 152 2109 1697
Fragments arranged by size:
3665 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2367 6403 160
Size: 872 1335 4036 1606
Fragments arranged by size:
4036 1606 1335 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7849
AvaI C'yCGr_G
Cuts at: 1870 1943 2907 3327 3530 3657 4347 5174 1870
Size: 73 964 420 203 127 690 827 4545
Fragments arranged by size:
4545 964 827 690 420 203 127 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3365 3398 3365
Size: 33 7816
BamHI G'GATC_C
Cuts at: 5179 5546 5179
Size: 367 7482
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2188 2477 3136 3458 3500
Size: 325 217 627 401 289 659 322 42
Cuts at: 3500 3748 4064 4426 5170 6625 618
Size: 248 316 362 744 1455 1842
Fragments arranged by size:
1842 1455 744 659 627 401 362 325
322 316 289 248 217 42
BanII G_rGCy'C
Cuts at: 1391 2288 2987 3026 4077 4193 4400 4677 5168
Size: 897 699 39 1051 116 207 277 491
Cuts at: 5168 1391
Size: 4072
Fragments arranged by size:
4072 1051 897 699 491 277 207 116
39
BbeI G_GCGC'C
Cuts at: 3140 3462 3504 3752 4068 3140
Size: 322 42 248 316 6921
Fragments arranged by size:
6921 322 316 248 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3234 3550 928
Size: 971 1335 316 5227
Fragments arranged by size:
5227 1335 971 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2542 2576 6040 6074 1903
Size: 34 605 34 3464 34 3678
Fragments arranged by size:
3678 3464 605 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5741 6951 5741
Size: 1210 6639
BclI T'GATC_A
Cuts at: 1548 4786 1548
Size: 3238 4611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3746 6460 136
Size: 107 122 71 631 605 2074 2714 1525
Fragments arranged by size:
2714 2074 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2140 7844 2140
Size: 5704 2145
BlpI GC'TnA_GC
Cuts at: 2565 4676 5266 2565
Size: 2111 590 5148
Fragments arranged by size:
5148 2111 590
Bme1580I G_kGCm'C
Cuts at: 1790 2193 2480 2613 2766 3026 4810 5910 7066
Size: 403 287 133 153 260 1784 1100 1156
Cuts at: 7066 1790
Size: 2573
Fragments arranged by size:
2573 1784 1156 1100 403 287 260 153
133
BmgBI CAC'GTC
Cuts at: 1318 2473 2533 3223 3430 1318
Size: 1155 60 690 207 5737
Fragments arranged by size:
5737 1155 690 207 60
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2981 3224 4122
Size: 416 53 83 399 24 1564 243 898
Cuts at: 4122 4404 4804 5580 6538 442
Size: 282 400 776 958 1753
Fragments arranged by size:
1753 1564 958 898 776 416 400 399
282 243 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7849
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4691
Size: 7849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2327 2339 2351 2597 2972 3759 4974
Size: 1007 81 12 12 246 375 787 1215
Cuts at: 4974 5081 6509 1239
Size: 107 1428 2579
Fragments arranged by size:
2579 1428 1215 1007 787 375 246 107
81 12 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2182 3221 5974 6839 7137 2182
Size: 1039 2753 865 298 2894
Fragments arranged by size:
2894 2753 1039 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6512 882
Size: 617 5013 2219
Fragments arranged by size:
5013 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2245 2620 3004 5146 5640 493
Size: 711 1041 375 384 2142 494 2702
Fragments arranged by size:
2702 2142 1041 711 494 384 375
BsaBI GATnn'nnATC
Cuts at: 4785 7843 4785
Size: 3058 4791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3137 3459 3501 3749
Size: 53 83 186 936 1604 322 42 248
Cuts at: 3749 4065 4912 5656 6038 6673 275
Size: 316 847 744 382 635 1451
Fragments arranged by size:
1604 1451 936 847 744 635 382 322
316 248 186 83 53 42
BsaWI w'CCGG_w
Cuts at: 4595 6282 6680 6954 7101 7512 4595
Size: 1687 398 274 147 411 4932
Fragments arranged by size:
4932 1687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7624
Fragments arranged by size:
7624 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2655 2667 3200 4451 1186
Size: 928 541 12 533 1251 4584
Fragments arranged by size:
4584 1251 928 541 533 12
BseYI C'CCAG_C
Cuts at: 2076 2700 3510 3972 4998 7052 7548 2076
Size: 624 810 462 1026 2054 496 2377
Fragments arranged by size:
2377 2054 1026 810 624 496 462
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 2555 3221 4412 1163
Size: 573 549 270 666 1191 4600
Fragments arranged by size:
4600 1191 666 573 549 270
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 5299 6060 7088 1540
Size: 275 186 1489 1809 761 1028 2301
Fragments arranged by size:
2301 1809 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2613 4119 4400 4572 4677 5910 5995 7066 2613
Size: 1506 281 172 105 1233 85 1071 3396
Fragments arranged by size:
3396 1506 1233 1071 281 172 105 85
BsmI GAATG_Cn'
Cuts at: 2459 4718 5360 5453 2459
Size: 2259 642 93 4855
Fragments arranged by size:
4855 2259 642 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3213 3213
Size: 7849
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2288 2480 2613 2766 2987 3026
Size: 399 403 95 192 133 153 221 39
Cuts at: 3026 4077 4119 4193 4400 4572 4677 4810 5168
Size: 1051 42 74 207 172 105 133 358
Cuts at: 5168 5910 5995 7066 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1051 742 403 399 358 221
207 192 172 153 133 133 105 95
85 74 42 39
BspEI T'CCGG_A
Cuts at: 7512 7512
Size: 7849
BspHI T'CATG_A
Cuts at: 4789 5738 7468 4789
Size: 949 1730 5170
Fragments arranged by size:
5170 1730 949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3187 3825 4337 5204 844
Size: 2343 638 512 867 3489
Fragments arranged by size:
3489 2343 867 638 512
BsrBI CCG'CTC
Cuts at: 1394 1823 3798 4095 5014 5736 1394
Size: 429 1975 297 919 722 3507
Fragments arranged by size:
3507 1975 919 722 429 297
BsrDI GCAATG_nn'
Cuts at: 65 1248 6344 6518 65
Size: 1183 5096 174 1396
Fragments arranged by size:
5096 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2714 3418 4595 6493 6680 1700
Size: 110 904 704 1177 1898 187 2869
Fragments arranged by size:
2869 1898 1177 904 704 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7849
BssHII G'CGCG_C
Cuts at: 1925 4352 1925
Size: 2427 5422
BssSI C'ACGA_G
Cuts at: 2825 5909 6921 2825
Size: 3084 1012 3753
Fragments arranged by size:
3753 3084 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2608 794
Size: 1814 6035
BstBI TT'CG_AA
Cuts at: 2007 6653 2007
Size: 4646 3203
BstEII G'GTnAC_C
Cuts at: 1184 3812 1184
Size: 2628 5221
BstXI CCAn_nnnn'nTGG
Cuts at: 2549 3019 4045 4817 2549
Size: 470 1026 772 5581
Fragments arranged by size:
5581 1026 772 470
BstYI r'GATC_y
Cuts at: 1972 2140 3540 4825 5179 5546 5931 5948 7400
Size: 168 1400 1285 354 367 385 17 1452
Cuts at: 7400 7486 7498 7844 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1400 1285 385 367 354 346
168 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2628 3837 4009 4068 513
Size: 552 1563 1209 172 59 4294
Fragments arranged by size:
4294 1563 1209 552 172 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4165 487
Size: 683 56 2939 4171
Fragments arranged by size:
4171 2939 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3209 5365 6159 6179 3043
Size: 166 2156 794 20 4713
Fragments arranged by size:
4713 2156 794 166 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7849
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5103 5138 529
Size: 35 4539 35 3240
Fragments arranged by size:
4539 3240 35 35
DraI TTT'AAA
Cuts at: 5153 5153
Size: 7849
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2780 4504 6856 809
Size: 507 1464 1724 2352 1802
Fragments arranged by size:
2352 1802 1724 1464 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3831 3957 4215
Size: 54 1425 50 275 18 2001 126 258
Cuts at: 4215 4230 4278 4336 5296 6185 7814 8
Size: 15 48 58 960 889 1629 43
Fragments arranged by size:
2001 1629 1425 960 889 275 258 126
58 54 50 48 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 5296 1537
Size: 275 3484 4090
Fragments arranged by size:
4090 3484 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2973 4835 5779 1353
Size: 1620 1862 944 3423
Fragments arranged by size:
3423 1862 1620 944
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3221 3818 4116 4332 4498 6436
Size: 1064 138 1632 597 298 216 166 1938
Cuts at: 6436 6810 6956 387
Size: 374 146 1280
Fragments arranged by size:
1938 1632 1280 1064 597 374 298 216
166 146 138
EcoICRI GAG'CTC
Cuts at: 4398 4675 4398
Size: 277 7572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2246 2327 2339 2351 2597 2872
Size: 393 525 89 81 12 12 246 275
Cuts at: 2872 2972 3085 3622 3650 3759 4974 5081 5912
Size: 100 113 537 28 109 1215 107 831
Cuts at: 5912 6509 7296 7564 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1215 831 787 597 537 525 393
275 268 246 113 109 107 100 89
81 28 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3756 3756
Size: 7849
EcoO109I rG'GnC_Cy
Cuts at: 2202 3016 3023 5044 2202
Size: 814 7 2021 5007
Fragments arranged by size:
5007 2021 814 7
EcoRI G'AATT_C
Cuts at: 5158 5536 5158
Size: 378 7471
EcoRV GAT'ATC
Cuts at: 4621 4621
Size: 7849
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2223 3262 2223
Size: 1039 6810
FspI TGC'GCA
Cuts at: 3042 3938 6355 3042
Size: 896 2417 4536
Fragments arranged by size:
4536 2417 896
HaeII r_GCGC'y
Cuts at: 1678 2427 3140 3462 3483 3504 3683 3752 4068
Size: 749 713 322 21 21 179 69 316
Cuts at: 4068 4126 5105 6996 1678
Size: 58 979 1891 2531
Fragments arranged by size:
2531 1891 979 749 713 322 316 179
69 58 21 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3164 3196 4610 4642 4817
Size: 32 135 32 1592 32 1414 32 175
Cuts at: 4817 4849 6490 6522 6564 6596 1373
Size: 32 1641 32 42 32 2626
Fragments arranged by size:
2626 1641 1592 1414 175 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3703 4690 4708 5193 5439 669
Size: 988 281 1765 987 18 485 246 3079
Fragments arranged by size:
3079 1765 988 987 485 281 246 18
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7849
HpaI GTT'AAC
Cuts at: 4708 5439 4708
Size: 731 7118
KasI G'GCGC_C
Cuts at: 3136 3458 3500 3748 4064 3136
Size: 322 42 248 316 6921
Fragments arranged by size:
6921 322 316 248 42
KpnI G_GTAC'C
Cuts at: 5174 5174
Size: 7849
MfeI C'AATT_G
Cuts at: 4837 5448 4837
Size: 611 7238
MluI A'CGCG_T
Cuts at: 7725 7725
Size: 7849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2949 3363 4653 4671 6963 7147 1305
Size: 1644 414 1290 18 2292 184 2007
Fragments arranged by size:
2292 2007 1644 1290 414 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4217 4280 10
Size: 54 1425 343 2385 63 3579
Fragments arranged by size:
3579 2385 1425 343 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2120 2738 2825 3149 3827 4043
Size: 648 861 93 618 87 324 678 216
Cuts at: 4043 4223 4388 4976 5807 6166 6325 518
Size: 180 165 588 831 359 159 2042
Fragments arranged by size:
2042 861 831 678 648 618 588 359
324 216 180 165 159 93 87
MspA1I CmG'CkG
Cuts at: 1606 2630 3628 3976 4054 4817 5265 5942 7090
Size: 1024 998 348 78 763 448 677 1148
Cuts at: 7090 7335 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1024 998 763 677 448 348
245 78
NarI GG'CG_CC
Cuts at: 3137 3459 3501 3749 4065 3137
Size: 322 42 248 316 6921
Fragments arranged by size:
6921 322 316 248 42
NcoI C'CATG_G
Cuts at: 513 1065 4009 513
Size: 552 2944 4353
Fragments arranged by size:
4353 2944 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7849
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7849
NotI GC'GGCC_GC
Cuts at: 5296 5296
Size: 7849
NspI r_CATG'y
Cuts at: 1132 1229 4946 5207 6752 1132
Size: 97 3717 261 1545 2229
Fragments arranged by size:
3717 2229 1545 261 97
PasI CC'CwG_GG
Cuts at: 1348 2542 4078 1348
Size: 1194 1536 5119
Fragments arranged by size:
5119 1536 1194
PciI A'CATG_T
Cuts at: 6748 6748
Size: 7849
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3682 1108
Size: 2574 5275
PfoI T'CCnGG_A
Cuts at: 2388 4722 2388
Size: 2334 5515
PmeI GTTT'AAAC
Cuts at: 5153 5153
Size: 7849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5918 5950 7431 7463 5918
Size: 32 1481 32 6304
Fragments arranged by size:
6304 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2202 3016 2202
Size: 814 7035
PshAI GACnn'nnGTC
Cuts at: 1613 4755 1613
Size: 3142 4707
PsiI TTA'TAA
Cuts at: 5419 5419
Size: 7849
PspOMI G'GGCC_C
Cuts at: 3022 3022
Size: 7849
PspXI vC'TCGA_Gb
Cuts at: 3530 3530
Size: 7849
PstI C_TGCA'G
Cuts at: 830 4544 5201 830
Size: 3714 657 3478
Fragments arranged by size:
3714 3478 657
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7849
PvuII CAG'CTG
Cuts at: 3628 4054 4817 3628
Size: 426 763 6660
Fragments arranged by size:
6660 763 426
SacI G_AGCT'C
Cuts at: 4400 4677 4400
Size: 277 7572
SacII CC_GC'GG
Cuts at: 2631 2631
Size: 7849
SalI G'TCGA_C
Cuts at: 1936 5191 1936
Size: 3255 4594
SbfI CC_TGCA'GG
Cuts at: 5201 5201
Size: 7849
ScaI AGT'ACT
Cuts at: 3066 6097 7809 3066
Size: 3031 1712 3106
Fragments arranged by size:
3106 3031 1712
SfcI C'TryA_G
Cuts at: 826 1045 3318 4540 5197 6332 7013 7204 826
Size: 219 2273 1222 657 1135 681 191 1471
Fragments arranged by size:
2273 1471 1222 1135 681 657 219 191
SfoI GGC'GCC
Cuts at: 3138 3460 3502 3750 4066 3138
Size: 322 42 248 316 6921
Fragments arranged by size:
6921 322 316 248 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7849
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7849
SmaI CCC'GGG
Cuts at: 1872 2909 3659 4349 5176 1872
Size: 1037 750 690 827 4545
Fragments arranged by size:
4545 1037 827 750 690
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2197 3236 3530 4532 5953 6854
Size: 197 926 254 1039 294 1002 1421 901
Cuts at: 6854 7116 820
Size: 262 1553
Fragments arranged by size:
1553 1421 1039 1002 926 901 294 262
254 197
SnaBI TAC'GTA
Cuts at: 493 5146 5640 493
Size: 4653 494 2702
Fragments arranged by size:
4653 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7849
SphI G_CATG'C
Cuts at: 1132 5207 1132
Size: 4075 3774
SrfI GCCC'GGGC
Cuts at: 4349 4349
Size: 7849
SspI AAT'ATT
Cuts at: 5 52 5773 5
Size: 47 5721 2081
Fragments arranged by size:
5721 2081 47
StuI AGG'CCT
Cuts at: 1517 2691 1517
Size: 1174 6675
StyI C'CwwG_G
Cuts at: 513 1065 2448 3012 4009 5288 513
Size: 552 1383 564 997 1279 3074
Fragments arranged by size:
3074 1383 1279 997 564 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2607 3303 4532 5876 5893 6046 6231
Size: 1255 1027 696 1229 1344 17 153 185
Cuts at: 6231 6676 325
Size: 445 1498
Fragments arranged by size:
1498 1344 1255 1229 1027 696 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2880 3064 6095
Size: 274 80 33 51 1443 903 184 3031
Cuts at: 6095 7807 96
Size: 1712 138
Fragments arranged by size:
3031 1712 1443 903 274 184 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3936 3968 7809 7841 3936
Size: 32 3841 32 3944
Fragments arranged by size:
3944 3841 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7849
XbaI T'CTAG_A
Cuts at: 5185 5185
Size: 7849
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2740 3683 4039 4225 2740
Size: 943 356 186 6364
Fragments arranged by size:
6364 943 356 186
XhoI C'TCGA_G
Cuts at: 1943 3530 1943
Size: 1587 6262
XmaI C'CCGG_G
Cuts at: 1870 2907 3657 4347 5174 1870
Size: 1037 750 690 827 4545
Fragments arranged by size:
4545 1037 827 750 690
XmnI GAAnn'nnTTC
Cuts at: 3635 5978 3635
Size: 2343 5506
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5657 276
Size: 53 83 186 936 4123 2468
Fragments arranged by size:
4123 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PsrI RsrII SanDI SapI SexAI
SfiI SwaI