Restriction map for
pFN21ASDA1399
(Circular) (Six-base) MAPSORT of: pFN21ASDA1399.seq Check: 7893 from: 1 to: 5959
pFN21ASDA1399 5959 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2410 2410
Size: 5959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 4022 5406 5674 1632
Size: 557 1833 1384 268 1917
Fragments arranged by size:
1917 1833 1384 557 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 3150 3248 1166
Size: 1984 98 3877
Fragments arranged by size:
3877 1984 98
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3120 1184
Size: 1936 4023
AlwNI CAG_nnn'CTG
Cuts at: 3702 5274 3702
Size: 1572 4387
ApaI G_GGCC'C
Cuts at: 2300 3104 2300
Size: 804 5155
ApaLI G'TGCA_C
Cuts at: 2581 4016 5172 2581
Size: 1435 1156 3368
Fragments arranged by size:
3368 1435 1156
ApoI r'AATT_y
Cuts at: 1493 3268 3494 3646 5755 1493
Size: 1775 226 152 2109 1697
Fragments arranged by size:
2109 1775 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 3284 1870
Size: 73 358 983 4545
Fragments arranged by size:
4545 983 358 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2187 2270 3080 3280 4735
Size: 325 217 627 400 83 810 200 1455
Cuts at: 4735 618
Size: 1842
Fragments arranged by size:
1842 1455 810 627 400 325 217 200
83
BanII G_rGCy'C
Cuts at: 1391 2300 2325 2555 2756 3104 3278 1391
Size: 909 25 230 201 348 174 4072
Fragments arranged by size:
4072 909 348 230 201 174 25
BbeI G_GCGC'C
Cuts at: 2191 2274 2191
Size: 83 5876
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2456 928
Size: 971 557 4431
Fragments arranged by size:
4431 971 557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2844 5954 2844
Size: 3110 2849
BlpI GC'TnA_GC
Cuts at: 2414 2549 3376 2414
Size: 135 827 4997
Fragments arranged by size:
4997 827 135
Bme1580I G_kGCm'C
Cuts at: 1790 2101 2300 2585 3104 4020 5176 1790
Size: 311 199 285 519 916 1156 2573
Fragments arranged by size:
2573 1156 916 519 311 285 199
BmgBI CAC'GTC
Cuts at: 1318 2662 2875 1318
Size: 1344 213 4402
Fragments arranged by size:
4402 1344 213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2386 2480 3690
Size: 416 53 83 399 24 969 94 1210
Cuts at: 3690 4648 442
Size: 958 1753
Fragments arranged by size:
1753 1210 969 958 416 399 94 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2311 2650 3011 4619 1239
Size: 1072 339 361 1608 2579
Fragments arranged by size:
2579 1608 1072 361 339
Bpu10I CC'TnA_GC
Cuts at: 3093 3093
Size: 5959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2120 2181 3205 4084 4949 5247 2120
Size: 61 1024 879 865 298 2832
Fragments arranged by size:
2832 1024 879 865 298 61
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 2894 3065 3181 4622 882
Size: 617 636 759 171 116 1441 2219
Fragments arranged by size:
2219 1441 759 636 617 171 116
BsaAI yAC'GTr
Cuts at: 493 1204 3256 3750 493
Size: 711 2052 494 2702
Fragments arranged by size:
2702 2052 711 494
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2188 2271 3766 4148
Size: 53 83 186 936 655 83 1495 382
Cuts at: 4148 4783 275
Size: 635 1451
Fragments arranged by size:
1495 1451 936 655 635 382 186 83
83 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2184 2416 3022 3049 3083 1186
Size: 937 61 232 606 27 34 4062
Fragments arranged by size:
4062 937 606 232 61 34 27
BseYI C'CCAG_C
Cuts at: 2101 2226 2386 5162 5658 2101
Size: 125 160 2776 496 2402
Fragments arranged by size:
2776 2402 496 160 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3152 1163
Size: 573 1416 3970
Fragments arranged by size:
3970 1416 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2585 4020 4105 5176 2585
Size: 1435 85 1071 3368
Fragments arranged by size:
3368 1435 1071 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2300 2325 2555 2585 2756 3104
Size: 399 311 199 25 230 30 171 348
Cuts at: 3104 3278 4020 4105 5176 1391
Size: 174 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 348 311 230 199
174 171 85 30 25
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3193 3234 3848 5578 3193
Size: 41 614 1730 3574
Fragments arranged by size:
3574 1730 614 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2410 3314 844
Size: 1566 904 3489
Fragments arranged by size:
3489 1566 904
BsrBI CCG'CTC
Cuts at: 1394 1823 2620 3846 1394
Size: 429 797 1226 3507
Fragments arranged by size:
3507 1226 797 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3116 4603 4790 1700
Size: 110 1306 1487 187 2869
Fragments arranged by size:
2869 1487 1306 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 2007 4763 2007
Size: 2756 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2844 3289 3656 4041 4058 5510 5596 5608
Size: 872 445 367 385 17 1452 86 12
Cuts at: 5608 5954 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 872 445 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3127 3127
Size: 5959
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5407
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2650 2752 487
Size: 683 56 1424 102 3694
Fragments arranged by size:
3694 1424 683 102 56
BtsI GCAGTG_nn'
Cuts at: 2425 2699 2960 3475 4269 4289 2425
Size: 274 261 515 794 20 4095
Fragments arranged by size:
4095 794 515 274 261 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2331 2366 529
Size: 35 1767 35 4122
Fragments arranged by size:
4122 1767 35 35
DraI TTT'AAA
Cuts at: 2929 3263 2929
Size: 334 5625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2244 3118 3406
Size: 54 1425 50 275 18 414 874 288
Cuts at: 3406 4295 5924 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 874 414 288 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2162 2765 2843 3060 3889 1353
Size: 809 603 78 217 829 3423
Fragments arranged by size:
3423 829 809 603 217 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2311 2650 3011 4022 4619 5406
Size: 393 557 122 339 361 1011 597 787
Cuts at: 5406 5674 1239
Size: 268 1524
Fragments arranged by size:
1524 1011 787 597 557 393 361 339
268 122
EcoO109I rG'GnC_Cy
Cuts at: 2296 2297 2499 2906 3100 3101 3131 2296
Size: 1 202 407 194 1 30 5124
Fragments arranged by size:
5124 407 202 194 30 1 1
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2191 2204 2274 2827 5106 1678
Size: 513 13 70 553 2279 2531
Fragments arranged by size:
2531 2279 553 513 70 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2443 2450 2475 2482 3216
Size: 32 135 32 871 7 25 7 734
Cuts at: 3216 3248 4600 4632 4674 4706 1373
Size: 32 1352 32 42 32 2626
Fragments arranged by size:
2626 1352 871 734 135 42 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2796 2947 3303 3549 669
Size: 988 281 858 151 356 246 3079
Fragments arranged by size:
3079 988 858 356 281 246 151
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 2187 2270 2187
Size: 83 5876
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2873 3150 3248 3917 4276 4435
Size: 648 861 846 277 98 669 359 159
Cuts at: 4435 518
Size: 2042
Fragments arranged by size:
2042 861 846 669 648 359 277 159
98
MspA1I CmG'CkG
Cuts at: 1606 2145 2562 3375 4052 5200 5445 1606
Size: 539 417 813 677 1148 245 2120
Fragments arranged by size:
2120 1148 813 677 539 417 245
NaeI GCC'GGC
Cuts at: 3118 3118
Size: 5959
NarI GG'CG_CC
Cuts at: 2188 2271 2188
Size: 83 5876
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NgoMIV G'CCGG_C
Cuts at: 3116 3116
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 2155 3317 4862 1132
Size: 97 926 1162 1545 2229
Fragments arranged by size:
2229 1545 1162 926 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2499 2906 2499
Size: 407 5552
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspOMI G'GGCC_C
Cuts at: 2296 3100 2296
Size: 804 5155
PstI C_TGCA'G
Cuts at: 830 2694 3311 830
Size: 1864 617 3478
Fragments arranged by size:
3478 1864 617
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SapI GCTCTTCn'nnn_
Cuts at: 2162 2162
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2690 3307 4442 5123 5314 826
Size: 219 1645 617 1135 681 191 1471
Fragments arranged by size:
1645 1471 1135 681 617 219 191
SfoI GGC'GCC
Cuts at: 2189 2272 2189
Size: 83 5876
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2135 2196 3184 4063 4964 5226
Size: 197 926 192 61 988 879 901 262
Cuts at: 5226 820
Size: 1553
Fragments arranged by size:
1553 988 926 901 879 262 197 192
61
SnaBI TAC'GTA
Cuts at: 493 3256 3750 493
Size: 2763 494 2702
Fragments arranged by size:
2763 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 2155 3317 1132
Size: 1023 1162 3774
Fragments arranged by size:
3774 1162 1023
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 3398 513
Size: 552 2333 3074
Fragments arranged by size:
3074 2333 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 2563 3056 3986 4003 4156 4341
Size: 1255 888 95 493 930 17 153 185
Cuts at: 4341 4786 325
Size: 445 1498
Fragments arranged by size:
1498 1255 930 888 493 445 185 153
95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4205 5917 96
Size: 274 80 33 51 1443 2228 1712 138
Fragments arranged by size:
2228 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2668 2700 2864 2896 2898 2930 5919 5951 2668
Size: 32 164 32 2 32 2989 32 2676
Fragments arranged by size:
2989 2676 164 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 2174 4088 2174
Size: 1914 4045
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI XcmI