Restriction map for
pFN21ASDA1397
(Circular) (Six-base) MAPSORT of: pFN21ASDA1397.seq Check: 3078 from: 1 to: 6763
pFN21ASDA1397 6763 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2355 2392 2581 2355
Size: 37 189 6537
Fragments arranged by size:
6537 189 37
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3207 4573 278
Size: 53 83 186 936 1671 1366 2468
Fragments arranged by size:
2468 1671 1366 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4106 1937
Size: 2169 4594
Acc65I G'GTAC_C
Cuts at: 4084 4084
Size: 6763
AclI AA'CG_TT
Cuts at: 1563 4890 5263 1563
Size: 3327 373 3063
Fragments arranged by size:
3327 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2999 4826 6210 6478 1632
Size: 1367 1827 1384 268 1917
Fragments arranged by size:
1917 1827 1384 1367 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6566
AflIII A'CryG_T
Cuts at: 1203 2534 5662 6639 1203
Size: 1331 3128 977 1327
Fragments arranged by size:
3128 1331 1327 977
AgeI A'CCGG_T
Cuts at: 5594 5594
Size: 6763
AhdI GACnn_n'nnGTC
Cuts at: 5492 5492
Size: 6763
AleI CACnn'nnGTG
Cuts at: 1166 3041 1166
Size: 1875 4888
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6763
AlwNI CAG_nnn'CTG
Cuts at: 2311 2569 2927 3160 4506 6078 2311
Size: 258 358 233 1346 1572 2996
Fragments arranged by size:
2996 1572 1346 358 258 233
ApaLI G'TGCA_C
Cuts at: 2899 4820 5976 2899
Size: 1921 1156 3686
Fragments arranged by size:
3686 1921 1156
ApoI r'AATT_y
Cuts at: 1493 2245 3842 3963 4072 4298 4450 6559 1493
Size: 752 1597 121 109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1597 752 226 152 121 109
AseI AT'TA_AT
Cuts at: 160 1032 3981 5317 160
Size: 872 2949 1336 1606
Fragments arranged by size:
2949 1606 1336 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6763
AvaI C'yCGr_G
Cuts at: 1870 1943 2812 3690 4088 1870
Size: 73 869 878 398 4545
Fragments arranged by size:
4545 878 869 398 73
BamHI G'GATC_C
Cuts at: 4093 4460 4093
Size: 367 6396
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4084 5539 618
Size: 325 217 627 2297 1455 1842
Fragments arranged by size:
2297 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2301 3708 3752 4082 1391
Size: 910 1407 44 330 4072
Fragments arranged by size:
4072 1407 910 330 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3792 3985 928
Size: 971 1893 193 3706
Fragments arranged by size:
3706 1893 971 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4954 4988 1903
Size: 34 3017 34 3678
Fragments arranged by size:
3678 3017 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4655 5865 4655
Size: 1210 5553
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6763
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5374 136
Size: 107 122 71 631 605 3702 1525
Fragments arranged by size:
3702 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6758 6758
Size: 6763
BlpI GC'TnA_GC
Cuts at: 4180 4180
Size: 6763
Bme1580I G_kGCm'C
Cuts at: 1790 2903 3432 4824 5980 1790
Size: 1113 529 1392 1156 2573
Fragments arranged by size:
2573 1392 1156 1113 529
BmgBI CAC'GTC
Cuts at: 1318 2632 3822 1318
Size: 1314 1190 4259
Fragments arranged by size:
4259 1314 1190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2765 2887 3604
Size: 416 53 83 399 24 1348 122 717
Cuts at: 3604 4494 5452 442
Size: 890 958 1753
Fragments arranged by size:
1753 1348 958 890 717 416 399 122
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3633 1056
Size: 2577 4186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3428 3553 3607 3905 5423 1239
Size: 2189 125 54 298 1518 2579
Fragments arranged by size:
2579 2189 1518 298 125 54
Bpu10I CC'TnA_GC
Cuts at: 2869 3699 3813 2869
Size: 830 114 5819
Fragments arranged by size:
5819 830 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2421 4888 5753 6051 2421
Size: 2467 865 298 3133
Fragments arranged by size:
3133 2467 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5426 882
Size: 617 3927 2219
Fragments arranged by size:
3927 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2319 2418 2535 3121 4060 4554 493
Size: 711 1115 99 117 586 939 494 2702
Fragments arranged by size:
2702 1115 939 711 586 494 117 99
BsaBI GATnn'nnATC
Cuts at: 6757 6757
Size: 6763
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3204 4570 4952 5587
Size: 53 83 186 936 1671 1366 382 635
Cuts at: 5587 275
Size: 1451
Fragments arranged by size:
1671 1451 1366 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5196 5594 5868 6015 6426 5196
Size: 398 274 147 411 5533
Fragments arranged by size:
5533 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3630 3660 1178
Size: 30 165 30 2227 30 4281
Fragments arranged by size:
4281 2227 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2193 1186
Size: 1007 5756
BseYI C'CCAG_C
Cuts at: 2927 2946 3069 3755 5966 6462 2927
Size: 19 123 686 2211 496 3228
Fragments arranged by size:
3228 2211 686 496 123 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2149 2795 3065 3326 3331 3909 1163
Size: 573 413 646 270 261 5 578 4017
Fragments arranged by size:
4017 646 578 573 413 270 261 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2392 2583 4213 4974 6002 1540
Size: 275 186 391 191 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 391 275 191 186
BsiHKAI G_wGCw'C
Cuts at: 2903 3021 3138 3708 4824 4909 5980 2903
Size: 118 117 570 1116 85 1071 3686
Fragments arranged by size:
3686 1116 1071 570 118 117 85
BsmI GAATG_Cn'
Cuts at: 2625 4274 4367 2625
Size: 1649 93 5021
Fragments arranged by size:
5021 1649 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2128 2128
Size: 6763
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 2903 3021 3138 3432 3708 3752
Size: 399 511 602 118 117 294 276 44
Cuts at: 3752 4082 4824 4909 5980 1391
Size: 330 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 602 511 399 330 294
276 118 117 85 44
BspEI T'CCGG_A
Cuts at: 6426 6426
Size: 6763
BspHI T'CATG_A
Cuts at: 4652 6382 4652
Size: 1730 5033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2355 2392 2581 4118 844
Size: 1511 37 189 1537 3489
Fragments arranged by size:
3489 1537 1511 189 37
BsrBI CCG'CTC
Cuts at: 1394 1823 2868 3337 4650 1394
Size: 429 1045 469 1313 3507
Fragments arranged by size:
3507 1313 1045 469 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5258 5432 65
Size: 1183 4010 174 1396
Fragments arranged by size:
4010 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3326 5407 5594 1700
Size: 110 1516 2081 187 2869
Fragments arranged by size:
2869 2081 1516 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6763
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6763
BssSI C'ACGA_G
Cuts at: 2642 3041 4823 5835 2642
Size: 399 1782 1012 3570
Fragments arranged by size:
3570 1782 1012 399
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6763
BstBI TT'CG_AA
Cuts at: 2007 5567 2007
Size: 3560 3203
BstEII G'GTnAC_C
Cuts at: 1184 3074 1184
Size: 1890 4873
BstYI r'GATC_y
Cuts at: 1972 3286 4093 4460 4845 4862 6314 6400 6412
Size: 1314 807 367 385 17 1452 86 12
Cuts at: 6412 6758 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1314 807 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2163 2392 2611 2856 2988 3488 3719
Size: 552 1098 229 219 245 132 500 231
Cuts at: 3719 3926 3969 513
Size: 207 43 3307
Fragments arranged by size:
3307 1098 552 500 245 231 229 219
207 132 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2337 3160 487
Size: 683 56 1111 823 4090
Fragments arranged by size:
4090 1111 823 683 56
BtsI GCAGTG_nn'
Cuts at: 2040 2065 3249 3343 4279 5073 5093 2040
Size: 25 1184 94 936 794 20 3710
Fragments arranged by size:
3710 1184 936 794 94 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6763
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2992 3027 529
Size: 35 2428 35 4265
Fragments arranged by size:
4265 2428 35 35
DraI TTT'AAA
Cuts at: 4067 4067
Size: 6763
DraIII CAC_nnn'GTG
Cuts at: 3142 3142
Size: 6763
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3203 5770 809
Size: 507 1887 2567 1802
Fragments arranged by size:
2567 1887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2313 2580
Size: 54 1425 50 275 18 330 153 267
Cuts at: 2580 3464 4210 5099 6728 8
Size: 884 746 889 1629 43
Fragments arranged by size:
1629 1425 889 884 746 330 275 267
153 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2580 4210 1537
Size: 275 768 1630 4090
Fragments arranged by size:
4090 1630 768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2975 4693 1353
Size: 1622 1718 3423
Fragments arranged by size:
3423 1718 1622
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5350 5724 5870 387
Size: 1064 138 3761 374 146 1280
Fragments arranged by size:
3761 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3706 3706
Size: 6763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2999 3428 3553 3607 3905 4826 5423
Size: 393 1367 429 125 54 298 921 597
Cuts at: 5423 6210 6478 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1367 921 787 597 429 393 298
268 125 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2693 2693
Size: 6763
EcoRI G'AATT_C
Cuts at: 4072 4450 4072
Size: 378 6385
FspI TGC'GCA
Cuts at: 3734 5269 3734
Size: 1535 5228
HaeII r_GCGC'y
Cuts at: 1678 3052 5910 1678
Size: 1374 2858 2531
Fragments arranged by size:
2858 2531 1374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2705 2737 2834 2866 3294
Size: 32 135 32 1133 32 97 32 428
Cuts at: 3294 3326 3928 3960 5404 5436 5478 5510 1373
Size: 32 602 32 1444 32 42 32 2626
Fragments arranged by size:
2626 1444 1133 602 428 135 97 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2386 4107 4353 669
Size: 988 281 448 1721 246 3079
Fragments arranged by size:
3079 1721 988 448 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6763
HpaI GTT'AAC
Cuts at: 4353 4353
Size: 6763
KpnI G_GTAC'C
Cuts at: 4088 4088
Size: 6763
MfeI C'AATT_G
Cuts at: 4362 4362
Size: 6763
MluI A'CGCG_T
Cuts at: 6639 6639
Size: 6763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 5877 6061 1305
Size: 786 3786 184 2007
Fragments arranged by size:
3786 2007 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 2315 10
Size: 54 1425 343 330 153 4458
Fragments arranged by size:
4458 1425 343 330 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2342 2771 3041 4721 5080 5239 518
Size: 648 1176 429 270 1680 359 159 2042
Fragments arranged by size:
2042 1680 1176 648 429 359 270 159
MspA1I CmG'CkG
Cuts at: 1606 2394 3157 3490 3552 3928 4179 4856 6004
Size: 788 763 333 62 376 251 677 1148
Cuts at: 6004 6249 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 788 763 677 376 333 251
245 62
NaeI GCC'GGC
Cuts at: 3328 3328
Size: 6763
NcoI C'CATG_G
Cuts at: 513 1065 2988 3969 513
Size: 552 1923 981 3307
Fragments arranged by size:
3307 1923 981 552
NdeI CA'TA_TG
Cuts at: 387 2543 387
Size: 2156 4607
NgoMIV G'CCGG_C
Cuts at: 3326 3326
Size: 6763
NheI G'CTAG_C
Cuts at: 1052 3629 1052
Size: 2577 4186
NotI GC'GGCC_GC
Cuts at: 4210 4210
Size: 6763
NspI r_CATG'y
Cuts at: 1132 1229 2668 4121 5666 1132
Size: 97 1439 1453 1545 2229
Fragments arranged by size:
2229 1545 1453 1439 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6763
PciI A'CATG_T
Cuts at: 5662 5662
Size: 6763
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 3069 1108
Size: 1203 758 4802
Fragments arranged by size:
4802 1203 758
PfoI T'CCnGG_A
Cuts at: 2084 2669 2084
Size: 585 6178
PmeI GTTT'AAAC
Cuts at: 4067 4067
Size: 6763
PmlI CAC'GTG
Cuts at: 2319 2418 2319
Size: 99 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4832 4864 6345 6377 4832
Size: 32 1481 32 5218
Fragments arranged by size:
5218 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2500 1613
Size: 887 5876
PsiI TTA'TAA
Cuts at: 4333 4333
Size: 6763
PstI C_TGCA'G
Cuts at: 830 3156 4115 830
Size: 2326 959 3478
Fragments arranged by size:
3478 2326 959
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6763
PvuII CAG'CTG
Cuts at: 3157 3552 3157
Size: 395 6368
SacI G_AGCT'C
Cuts at: 3708 3708
Size: 6763
SacII CC_GC'GG
Cuts at: 2395 3491 3929 2395
Size: 1096 438 5229
Fragments arranged by size:
5229 1096 438
SalI G'TCGA_C
Cuts at: 1936 4105 1936
Size: 2169 4594
SbfI CC_TGCA'GG
Cuts at: 4115 4115
Size: 6763
ScaI AGT'ACT
Cuts at: 2230 5011 6723 2230
Size: 2781 1712 2270
Fragments arranged by size:
2781 2270 1712
SexAI A'CCwGG_T
Cuts at: 4033 4033
Size: 6763
SfcI C'TryA_G
Cuts at: 826 1045 3152 4111 5246 5927 6118 826
Size: 219 2107 959 1135 681 191 1471
Fragments arranged by size:
2107 1471 1135 959 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6763
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6763
SmaI CCC'GGG
Cuts at: 1872 3692 4090 1872
Size: 1820 398 4545
Fragments arranged by size:
4545 1820 398
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2436 4867 5768 6030 820
Size: 197 926 493 2431 901 262 1553
Fragments arranged by size:
2431 1553 926 901 493 262 197
SnaBI TAC'GTA
Cuts at: 493 4060 4554 493
Size: 3567 494 2702
Fragments arranged by size:
3567 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6763
SphI G_CATG'C
Cuts at: 1132 4121 1132
Size: 2989 3774
SspI AAT'ATT
Cuts at: 5 52 4687 5
Size: 47 4635 2081
Fragments arranged by size:
4635 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6763
StyI C'CwwG_G
Cuts at: 513 1065 2988 3650 3969 4202 513
Size: 552 1923 662 319 233 3074
Fragments arranged by size:
3074 1923 662 552 319 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3655 3694 4790 4807 4960 5145 5590
Size: 1255 2075 39 1096 17 153 185 445
Cuts at: 5590 325
Size: 1498
Fragments arranged by size:
2075 1498 1255 1096 445 185 153 39
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2228 5009 6721
Size: 274 80 33 51 1443 251 2781 1712
Cuts at: 6721 96
Size: 138
Fragments arranged by size:
2781 1712 1443 274 251 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2080 2112 2489 2521 3128 3160 3630 3662 6723
Size: 32 377 32 607 32 470 32 3061
Cuts at: 6723 6755 2080
Size: 32 2088
Fragments arranged by size:
3061 2088 607 470 377 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2654 3308 1316
Size: 1338 654 4771
Fragments arranged by size:
4771 1338 654
XbaI T'CTAG_A
Cuts at: 4099 4099
Size: 6763
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6763
XmaI C'CCGG_G
Cuts at: 1870 3690 4088 1870
Size: 1820 398 4545
Fragments arranged by size:
4545 1820 398
XmnI GAAnn'nnTTC
Cuts at: 4892 4892
Size: 6763
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3205 4571 276
Size: 53 83 186 936 1671 1366 2468
Fragments arranged by size:
2468 1671 1366 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI
SfoI SrfI SwaI XcmI