Restriction map for
pFN21ASDA1388
(Circular) (Six-base) MAPSORT of: pFN21ASDA1388.seq Check: 132 from: 1 to: 6475
pFN21ASDA1388 6475 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3186 3186
Size: 6475
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4285 278
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3818 1937
Size: 1881 4594
Acc65I G'GTAC_C
Cuts at: 3796 3796
Size: 6475
AclI AA'CG_TT
Cuts at: 1563 4602 4975 1563
Size: 3039 373 3063
Fragments arranged by size:
3063 3039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2722 4538 5922 6190 1632
Size: 1090 1816 1384 268 1917
Fragments arranged by size:
1917 1816 1384 1090 268
AfeI AGC'GCT
Cuts at: 3671 3671
Size: 6475
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6278
AflIII A'CryG_T
Cuts at: 1203 2850 2892 3513 5374 6351 1203
Size: 1647 42 621 1861 977 1327
Fragments arranged by size:
1861 1647 1327 977 621 42
AgeI A'CCGG_T
Cuts at: 2696 5306 2696
Size: 2610 3865
AhdI GACnn_n'nnGTC
Cuts at: 5204 5204
Size: 6475
AleI CACnn'nnGTG
Cuts at: 1166 2697 3481 1166
Size: 1531 784 4160
Fragments arranged by size:
4160 1531 784
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2198 3732 1184
Size: 1014 1534 3927
Fragments arranged by size:
3927 1534 1014
AlwNI CAG_nnn'CTG
Cuts at: 2311 4218 5790 2311
Size: 1907 1572 2996
Fragments arranged by size:
2996 1907 1572
ApaLI G'TGCA_C
Cuts at: 2338 2716 2759 2941 3197 3626 4532 5688 2338
Size: 378 43 182 256 429 906 1156 3125
Fragments arranged by size:
3125 1156 906 429 378 256 182 43
ApoI r'AATT_y
Cuts at: 1493 3784 4010 4162 6271 1493
Size: 2291 226 152 2109 1697
Fragments arranged by size:
2291 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 2863 3206 3800 1870
Size: 73 920 343 594 4545
Fragments arranged by size:
4545 920 594 343 73
BamHI G'GATC_C
Cuts at: 2688 3805 4172 2688
Size: 1117 367 4991
Fragments arranged by size:
4991 1117 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3531 3740 3796 5251 618
Size: 325 217 627 1744 209 56 1455 1842
Fragments arranged by size:
1842 1744 1455 627 325 217 209 56
BanII G_rGCy'C
Cuts at: 1391 2673 3132 3794 1391
Size: 1282 459 662 4072
Fragments arranged by size:
4072 1282 662 459
BbeI G_GCGC'C
Cuts at: 3744 3744
Size: 6475
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2822 928
Size: 971 923 4581
Fragments arranged by size:
4581 971 923
BbvCI CC'TCA_GC
Cuts at: 2085 2532 2085
Size: 447 6028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4666 4700 1903
Size: 34 2729 34 3678
Fragments arranged by size:
3678 2729 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4367 5577 4367
Size: 1210 5265
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3072 3240 3285
Size: 107 122 71 631 605 1400 168 45
Cuts at: 3285 3728 5086 136
Size: 443 1358 1525
Fragments arranged by size:
1525 1400 1358 631 605 443 168 122
107 71 45
BglII A'GATC_T
Cuts at: 2355 6470 2355
Size: 4115 2360
BlpI GC'TnA_GC
Cuts at: 2403 3892 2403
Size: 1489 4986
Bme1580I G_kGCm'C
Cuts at: 1790 2141 2342 2720 2763 2945 3068 3201 3227
Size: 351 201 378 43 182 123 133 26
Cuts at: 3227 3314 3488 3572 3630 4536 5692 1790
Size: 87 174 84 58 906 1156 2573
Fragments arranged by size:
2573 1156 906 378 351 201 182 174
133 123 87 84 58 43 26
BmgBI CAC'GTC
Cuts at: 1318 3682 1318
Size: 2364 4111
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4206 5164 442
Size: 416 53 83 399 24 2789 958 1753
Fragments arranged by size:
2789 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 5135 1239
Size: 1163 2733 2579
Fragments arranged by size:
2733 2579 1163
Bpu10I CC'TnA_GC
Cuts at: 2085 2532 2085
Size: 447 6028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 3464 4600 5465 5763 2336
Size: 1128 1136 865 298 3048
Fragments arranged by size:
3048 1136 1128 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5138 882
Size: 617 3639 2219
Fragments arranged by size:
3639 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3196 3772 4266 493
Size: 711 1992 576 494 2702
Fragments arranged by size:
2702 1992 711 576 494
BsaBI GATnn'nnATC
Cuts at: 6469 6469
Size: 6475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3741 4282 4664 5299
Size: 53 83 186 936 2208 541 382 635
Cuts at: 5299 275
Size: 1451
Fragments arranged by size:
2208 1451 936 635 541 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2696 4908 5306 5580 5727 6138 2696
Size: 2212 398 274 147 411 3033
Fragments arranged by size:
3033 2212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2116 2146 2375 2405 2462
Size: 30 165 30 713 30 229 30 57
Cuts at: 2462 2492 1178
Size: 30 5161
Fragments arranged by size:
5161 713 229 165 57 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3140 3418 1186
Size: 1954 278 4243
Fragments arranged by size:
4243 1954 278
BseYI C'CCAG_C
Cuts at: 3170 5678 6174 3170
Size: 2508 496 3471
Fragments arranged by size:
3471 2508 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 3385 3464 1163
Size: 573 681 968 79 4174
Fragments arranged by size:
4174 968 681 573 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3294 3925 4686 5714 1540
Size: 275 186 1293 631 761 1028 2301
Fragments arranged by size:
2301 1293 1028 761 631 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2304 2342 2388 2673 2720 2763 2874 2945
Size: 180 38 46 285 47 43 111 71
Cuts at: 2945 3201 3630 3702 4536 4621 5692 2124
Size: 256 429 72 834 85 1071 2907
Fragments arranged by size:
2907 1071 834 429 285 256 180 111
85 72 71 47 46 43 38
BsmI GAATG_Cn'
Cuts at: 3986 4079 3986
Size: 93 6382
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2141 2304 2342 2388 2673 2720
Size: 399 334 17 163 38 46 285 47
Cuts at: 2720 2763 2874 2945 3068 3132 3201 3227 3314
Size: 43 111 71 123 64 69 26 87
Cuts at: 3314 3488 3572 3630 3702 3794 4536 4621 5692
Size: 174 84 58 72 92 742 85 1071
Cuts at: 5692 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 334 285 174 163
123 111 92 87 85 84 72 71
69 64 58 47 46 43 38 26
17
BspEI T'CCGG_A
Cuts at: 6138 6138
Size: 6475
BspHI T'CATG_A
Cuts at: 4364 6094 4364
Size: 1730 4745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2385 3018 3186 3531 3830 844
Size: 1541 633 168 345 299 3489
Fragments arranged by size:
3489 1541 633 345 299 168
BsrBI CCG'CTC
Cuts at: 1394 1823 2328 2686 2788 2832 2959 3043 3298
Size: 429 505 358 102 44 127 84 255
Cuts at: 3298 4362 1394
Size: 1064 3507
Fragments arranged by size:
3507 1064 505 429 358 255 127 102
84 44
BsrDI GCAATG_nn'
Cuts at: 65 1248 4970 5144 65
Size: 1183 3722 174 1396
Fragments arranged by size:
3722 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2696 3105 3534 5119 5306 1700
Size: 110 886 409 429 1585 187 2869
Fragments arranged by size:
2869 1585 886 429 409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6475
BssHII G'CGCG_C
Cuts at: 1925 3337 3705 1925
Size: 1412 368 4695
Fragments arranged by size:
4695 1412 368
BssSI C'ACGA_G
Cuts at: 4535 5547 4535
Size: 1012 5463
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2128 2758 2895 3408 794
Size: 1334 630 137 513 3861
Fragments arranged by size:
3861 1334 630 513 137
BstBI TT'CG_AA
Cuts at: 2007 5279 2007
Size: 3272 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6475
BstYI r'GATC_y
Cuts at: 1972 2355 2688 3805 4172 4557 4574 6026 6112
Size: 383 333 1117 367 385 17 1452 86
Cuts at: 6112 6124 6470 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1117 385 383 367 346 333
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3069 3726 513
Size: 552 2004 657 3262
Fragments arranged by size:
3262 2004 657 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5736
Fragments arranged by size:
5736 683 56
BtsI GCAGTG_nn'
Cuts at: 3991 4785 4805 3991
Size: 794 20 5661
Fragments arranged by size:
5661 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6440
DraI TTT'AAA
Cuts at: 3779 3779
Size: 6475
DraIII CAC_nnn'GTG
Cuts at: 2346 2724 2758 2842 2346
Size: 378 34 84 5979
Fragments arranged by size:
5979 378 84 34
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5482 809
Size: 507 4166 1802
Fragments arranged by size:
4166 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3291 3922 4811
Size: 54 1425 50 275 18 1461 631 889
Cuts at: 4811 6440 8
Size: 1629 43
Fragments arranged by size:
1629 1461 1425 889 631 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3291 3922 1537
Size: 275 1479 631 4090
Fragments arranged by size:
4090 1479 631 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3221 3436 4405 1353
Size: 1868 215 969 3423
Fragments arranged by size:
3423 1868 969 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 5062 5436 5582 387
Size: 1064 138 570 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 570 374 146 138
EcoICRI GAG'CTC
Cuts at: 2671 2671
Size: 6475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2402 2722 4538 5135 5922 6190 1239
Size: 393 770 320 1816 597 787 268 1524
Fragments arranged by size:
1816 1524 787 770 597 393 320 268
EcoO109I rG'GnC_Cy
Cuts at: 2246 2246
Size: 6475
EcoRI G'AATT_C
Cuts at: 3784 4162 3784
Size: 378 6097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 6475
FspI TGC'GCA
Cuts at: 2803 4981 2803
Size: 2178 4297
HaeII r_GCGC'y
Cuts at: 1678 3039 3673 3744 5622 1678
Size: 1361 634 71 1878 2531
Fragments arranged by size:
2531 1878 1361 634 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5116 5148 5190 5222 1373
Size: 32 135 32 3544 32 42 32 2626
Fragments arranged by size:
3544 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3819 4065 669
Size: 988 281 1881 246 3079
Fragments arranged by size:
3079 1881 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2734 748
Size: 1986 4489
HpaI GTT'AAC
Cuts at: 4065 4065
Size: 6475
KasI G'GCGC_C
Cuts at: 3740 3740
Size: 6475
KpnI G_GTAC'C
Cuts at: 3800 3800
Size: 6475
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 3513 6351 3513
Size: 2838 3637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2007 2646 2901 5589 5773 1305
Size: 702 639 255 2688 184 2007
Fragments arranged by size:
2688 2007 702 639 255 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4653
Fragments arranged by size:
4653 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2335 2697 3481 4433 4792 4951 518
Size: 648 1169 362 784 952 359 159 2042
Fragments arranged by size:
2042 1169 952 784 648 362 359 159
MspA1I CmG'CkG
Cuts at: 1606 2531 2540 2631 2886 2970 3364 3453 3529
Size: 925 9 91 255 84 394 89 76
Cuts at: 3529 3598 3728 3734 3891 4568 5716 5961 1606
Size: 69 130 6 157 677 1148 245 2120
Fragments arranged by size:
2120 1148 925 677 394 255 245 157
130 91 89 84 76 69 9 6
NarI GG'CG_CC
Cuts at: 3741 3741
Size: 6475
NcoI C'CATG_G
Cuts at: 513 1065 3069 513
Size: 552 2004 3919
Fragments arranged by size:
3919 2004 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 3922 3922
Size: 6475
NspI r_CATG'y
Cuts at: 1132 1229 2854 3833 5378 1132
Size: 97 1625 979 1545 2229
Fragments arranged by size:
2229 1625 1545 979 97
PasI CC'CwG_GG
Cuts at: 1348 2155 1348
Size: 807 5668
PciI A'CATG_T
Cuts at: 2850 5374 2850
Size: 2524 3951
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 1108
Size: 1203 5272
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PmlI CAC'GTG
Cuts at: 3196 3196
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 4544 4576 6057 6089 3629
Size: 32 883 32 1481 32 4015
Fragments arranged by size:
4015 1481 883 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6475
PsiI TTA'TAA
Cuts at: 4045 4045
Size: 6475
PstI C_TGCA'G
Cuts at: 830 2187 2205 2229 2934 3354 3528 3827 830
Size: 1357 18 24 705 420 174 299 3478
Fragments arranged by size:
3478 1357 705 420 299 174 24 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
PvuII CAG'CTG
Cuts at: 2531 2631 3364 2531
Size: 100 733 5642
Fragments arranged by size:
5642 733 100
SacI G_AGCT'C
Cuts at: 2673 2673
Size: 6475
SacII CC_GC'GG
Cuts at: 3729 3729
Size: 6475
SalI G'TCGA_C
Cuts at: 1936 3817 1936
Size: 1881 4594
SbfI CC_TGCA'GG
Cuts at: 2229 3827 2229
Size: 1598 4877
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SexAI A'CCwGG_T
Cuts at: 2754 2838 3747 2754
Size: 84 909 5482
Fragments arranged by size:
5482 909 84
SfcI C'TryA_G
Cuts at: 826 1045 2183 2201 2225 2626 2930 3350 3524
Size: 219 1138 18 24 401 304 420 174
Cuts at: 3524 3823 4958 5639 5830 826
Size: 299 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 681 420 401 304 299
219 191 174 24 18
SfoI GGC'GCC
Cuts at: 3742 3742
Size: 6475
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 2696 3105 3534 1700
Size: 996 409 429 4641
Fragments arranged by size:
4641 996 429 409
SmaI CCC'GGG
Cuts at: 1872 3802 1872
Size: 1930 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 3206 3479 4579 5480 5742
Size: 197 926 408 855 273 1100 901 262
Cuts at: 5742 820
Size: 1553
Fragments arranged by size:
1553 1100 926 901 855 408 273 262
197
SnaBI TAC'GTA
Cuts at: 493 3772 4266 493
Size: 3279 494 2702
Fragments arranged by size:
3279 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6475
SphI G_CATG'C
Cuts at: 1132 3833 1132
Size: 2701 3774
SspI AAT'ATT
Cuts at: 5 52 4399 5
Size: 47 4347 2081
Fragments arranged by size:
4347 2081 47
StuI AGG'CCT
Cuts at: 1517 2990 3329 3587 1517
Size: 1473 339 258 4405
Fragments arranged by size:
4405 1473 339 258
StyI C'CwwG_G
Cuts at: 513 1065 3069 3914 513
Size: 552 2004 845 3074
Fragments arranged by size:
3074 2004 845 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3137 4502 4519 4672 4857 5302 325
Size: 1255 1557 1365 17 153 185 445 1498
Fragments arranged by size:
1557 1498 1365 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4721 6433 96
Size: 274 80 33 51 1443 2744 1712 138
Fragments arranged by size:
2744 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2116 2148 2375 2407 2462 2494 6435 6467 2116
Size: 32 227 32 55 32 3941 32 2124
Fragments arranged by size:
3941 2124 227 55 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6475
XbaI T'CTAG_A
Cuts at: 3811 3811
Size: 6475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2377 2377
Size: 6475
XhoI C'TCGA_G
Cuts at: 1943 3206 1943
Size: 1263 5212
XmaI C'CCGG_G
Cuts at: 1870 3800 1870
Size: 1930 4545
XmnI GAAnn'nnTTC
Cuts at: 2414 2819 2990 3503 4604 2414
Size: 405 171 513 1101 4285
Fragments arranged by size:
4285 1101 513 405 171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4283 276
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI