Restriction map for
pFN21ASDA1383
(Circular) (Six-base) MAPSORT of: pFN21ASDA1383.seq Check: 6179 from: 1 to: 7444
pFN21ASDA1383 7444 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5254 278
Size: 53 83 186 936 3718 2468
Fragments arranged by size:
3718 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4787 1937
Size: 2850 4594
Acc65I G'GTAC_C
Cuts at: 4765 4765
Size: 7444
AclI AA'CG_TT
Cuts at: 1563 5571 5944 1563
Size: 4008 373 3063
Fragments arranged by size:
4008 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3378 3395 3717 4136 4173 5507 6891 7159
Size: 1746 17 322 419 37 1334 1384 268
Cuts at: 7159 1632
Size: 1917
Fragments arranged by size:
1917 1746 1384 1334 419 322 268 37
17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7247
AflIII A'CryG_T
Cuts at: 1203 2837 3164 6343 7320 1203
Size: 1634 327 3179 977 1327
Fragments arranged by size:
3179 1634 1327 977 327
AgeI A'CCGG_T
Cuts at: 6275 6275
Size: 7444
AhdI GACnn_n'nnGTC
Cuts at: 6173 6173
Size: 7444
AleI CACnn'nnGTG
Cuts at: 1166 4265 1166
Size: 3099 4345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7444
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4596 4628 4596
Size: 32 7412
AlwNI CAG_nnn'CTG
Cuts at: 2416 2674 2849 5187 6759 2416
Size: 258 175 2338 1572 3101
Fragments arranged by size:
3101 2338 1572 258 175
ApaLI G'TGCA_C
Cuts at: 5501 6657 5501
Size: 1156 6288
ApoI r'AATT_y
Cuts at: 1493 3626 3633 3889 4352 4753 4979 5131 7240
Size: 2133 7 256 463 401 226 152 2109
Cuts at: 7240 1493
Size: 1697
Fragments arranged by size:
2133 2109 1697 463 401 256 226 152
7
AseI AT'TA_AT
Cuts at: 160 1032 3189 5998 160
Size: 872 2157 2809 1606
Fragments arranged by size:
2809 2157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
AvaI C'yCGr_G
Cuts at: 1870 1943 4769 1870
Size: 73 2826 4545
Fragments arranged by size:
4545 2826 73
BamHI G'GATC_C
Cuts at: 4774 5141 4774
Size: 367 7077
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2391 3473 4765 6220 618
Size: 325 217 627 604 1082 1292 1455 1842
Fragments arranged by size:
1842 1455 1292 1082 627 604 325 217
BanII G_rGCy'C
Cuts at: 1391 3780 4501 4763 1391
Size: 2389 721 262 4072
Fragments arranged by size:
4072 2389 721 262
BbeI G_GCGC'C
Cuts at: 2395 2395
Size: 7444
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3401 928
Size: 971 1502 4971
Fragments arranged by size:
4971 1502 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3317 3351 5635 5669 1903
Size: 34 1380 34 2284 34 3678
Fragments arranged by size:
3678 2284 1380 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4721 5336 6546 4721
Size: 615 1210 5619
Fragments arranged by size:
5619 1210 615
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7444
BfrBI ATG'CAT
Cuts at: 3722 3722
Size: 7444
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2389 6055 136
Size: 107 122 71 631 605 717 3666 1525
Fragments arranged by size:
3666 1525 717 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7439 7439
Size: 7444
BlpI GC'TnA_GC
Cuts at: 2588 4861 2588
Size: 2273 5171
Bme1580I G_kGCm'C
Cuts at: 1790 2186 3478 5505 6661 1790
Size: 396 1292 2027 1156 2573
Fragments arranged by size:
2573 2027 1292 1156 396
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 2547 5175
Size: 416 53 83 399 24 666 464 2628
Cuts at: 5175 6133 442
Size: 958 1753
Fragments arranged by size:
2628 1753 958 666 464 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 6104 1239
Size: 839 4026 2579
Fragments arranged by size:
4026 2579 839
Bpu10I CC'TnA_GC
Cuts at: 4307 4307
Size: 7444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 3733 4485 5569 6434 6732 2618
Size: 1115 752 1084 865 298 3330
Fragments arranged by size:
3330 1115 1084 865 752 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2900 6107 882
Size: 617 1401 3207 2219
Fragments arranged by size:
3207 2219 1401 617
BsaAI yAC'GTr
Cuts at: 493 1204 4741 5235 493
Size: 711 3537 494 2702
Fragments arranged by size:
3537 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7438 7438
Size: 7444
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2392 2399 5251 5633
Size: 53 83 186 936 859 7 2852 382
Cuts at: 5633 6268 275
Size: 635 1451
Fragments arranged by size:
2852 1451 936 859 635 382 186 83
53 7
BsaWI w'CCGG_w
Cuts at: 2477 5877 6275 6549 6696 7107 2477
Size: 3400 398 274 147 411 2814
Fragments arranged by size:
3400 2814 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 2625 2655 3924
Size: 30 165 30 747 30 445 30 1269
Cuts at: 3924 3954 1178
Size: 30 4668
Fragments arranged by size:
4668 1269 747 445 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2153 2643 2646 2916 1186
Size: 958 9 490 3 270 5714
Fragments arranged by size:
5714 958 490 270 9 3
BseYI C'CCAG_C
Cuts at: 2655 2722 3058 3779 6647 7143 2655
Size: 67 336 721 2868 496 2956
Fragments arranged by size:
2956 2868 721 496 336 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2955 1163
Size: 573 582 637 5652
Fragments arranged by size:
5652 637 582 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2293 2472 4894 5655 6683 1540
Size: 275 186 292 179 2422 761 1028 2301
Fragments arranged by size:
2422 2301 1028 761 292 275 186 179
BsiHKAI G_wGCw'C
Cuts at: 4501 5505 5590 6661 4501
Size: 1004 85 1071 5284
Fragments arranged by size:
5284 1071 1004 85
BsmI GAATG_Cn'
Cuts at: 3010 4955 5048 3010
Size: 1945 93 5406
Fragments arranged by size:
5406 1945 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 3478 3780 4501 4763 5505 5590
Size: 399 396 1292 302 721 262 742 85
Cuts at: 5590 6661 1391
Size: 1071 2174
Fragments arranged by size:
2174 1292 1071 742 721 399 396 302
262 85
BspEI T'CCGG_A
Cuts at: 2477 7107 2477
Size: 4630 2814
BspHI T'CATG_A
Cuts at: 4198 5333 7063 4198
Size: 1135 1730 4579
Fragments arranged by size:
4579 1730 1135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4593 4799 844
Size: 3749 206 3489
Fragments arranged by size:
3749 3489 206
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 2533 2602 2636 5331 1394
Size: 429 221 489 69 34 2695 3507
Fragments arranged by size:
3507 2695 489 429 221 69 34
BsrDI GCAATG_nn'
Cuts at: 65 1248 2544 2860 4657 4691 5939 6113 65
Size: 1183 1296 316 1797 34 1248 174 1396
Fragments arranged by size:
1797 1396 1296 1248 1183 316 174 34
BsrFI r'CCGG_y
Cuts at: 1700 1810 2340 2467 3351 6088 6275 1700
Size: 110 530 127 884 2737 187 2869
Fragments arranged by size:
2869 2737 884 530 187 127 110
BsrGI T'GTAC_A
Cuts at: 96 4336 96
Size: 4240 3204
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7444
BssSI C'ACGA_G
Cuts at: 5504 6516 5504
Size: 1012 6432
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2304 794
Size: 1510 5934
BstBI TT'CG_AA
Cuts at: 2007 6248 2007
Size: 4241 3203
BstEII G'GTnAC_C
Cuts at: 1184 4329 1184
Size: 3145 4299
BstXI CCAn_nnnn'nTGG
Cuts at: 2224 2224
Size: 7444
BstYI r'GATC_y
Cuts at: 1972 4774 5141 5526 5543 6995 7081 7093 7439
Size: 2802 367 385 17 1452 86 12 346
Cuts at: 7439 1972
Size: 1977
Fragments arranged by size:
2802 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3012 3012
Size: 7444
BtgI C'CryG_G
Cuts at: 513 1065 2022 2190 2267 513
Size: 552 957 168 77 5690
Fragments arranged by size:
5690 957 552 168 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6705
Fragments arranged by size:
6705 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 2335 3475 4960 5754 5774 2124
Size: 211 1140 1485 794 20 3794
Fragments arranged by size:
3794 1485 1140 794 211 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7409
DraI TTT'AAA
Cuts at: 3543 4748 3543
Size: 1205 6239
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6451 809
Size: 507 5135 1802
Fragments arranged by size:
5135 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 2469 4891
Size: 54 1425 50 275 18 609 30 2422
Cuts at: 4891 5780 7409 8
Size: 889 1629 43
Fragments arranged by size:
2422 1629 1425 889 609 275 54 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2469 4891 1537
Size: 275 657 2422 4090
Fragments arranged by size:
4090 2422 657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2130 2315 5374 1353
Size: 701 76 185 3059 3423
Fragments arranged by size:
3423 3059 701 185 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2189 2303 6031 6405 6551 387
Size: 1064 138 600 114 3728 374 146 1280
Fragments arranged by size:
3728 1280 1064 600 374 146 138 114
EcoICRI GAG'CTC
Cuts at: 4499 4499
Size: 7444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 3378 3395 3717 4136 4173 5507
Size: 393 446 1300 17 322 419 37 1334
Cuts at: 5507 6104 6891 7159 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1334 1300 787 597 446 419 393
322 268 37 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2879 2879
Size: 7444
EcoO109I rG'GnC_Cy
Cuts at: 2421 2421
Size: 7444
EcoRI G'AATT_C
Cuts at: 3633 4753 5131 3633
Size: 1120 378 5946
Fragments arranged by size:
5946 1120 378
FseI GG_CCGG'CC
Cuts at: 2471 2471
Size: 7444
FspI TGC'GCA
Cuts at: 5950 5950
Size: 7444
HaeII r_GCGC'y
Cuts at: 1678 2253 2395 6591 1678
Size: 575 142 4196 2531
Fragments arranged by size:
4196 2531 575 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 2625 2657 3305
Size: 32 135 32 578 32 443 32 648
Cuts at: 3305 3337 4118 4150 4207 4239 4379 4411 6085
Size: 32 781 32 57 32 140 32 1674
Cuts at: 6085 6117 6159 6191 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1674 781 648 578 443 140 135
57 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3991 4788 5034 669
Size: 988 281 2053 797 246 3079
Fragments arranged by size:
3079 2053 988 797 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7444
HpaI GTT'AAC
Cuts at: 5034 5034
Size: 7444
KasI G'GCGC_C
Cuts at: 2391 2391
Size: 7444
KpnI G_GTAC'C
Cuts at: 4769 4769
Size: 7444
MfeI C'AATT_G
Cuts at: 5043 5043
Size: 7444
MluI A'CGCG_T
Cuts at: 7320 7320
Size: 7444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3997 4511 6558 6742 1305
Size: 2692 514 2047 184 2007
Fragments arranged by size:
2692 2047 2007 514 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 10
Size: 54 1425 343 609 5013
Fragments arranged by size:
5013 1425 609 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4052 4265 5402 5761 5920 518
Size: 648 2886 213 1137 359 159 2042
Fragments arranged by size:
2886 2042 1137 648 359 213 159
MspA1I CmG'CkG
Cuts at: 1606 2111 2380 2674 2722 4860 5537 6685 6930
Size: 505 269 294 48 2138 677 1148 245
Cuts at: 6930 1606
Size: 2120
Fragments arranged by size:
2138 2120 1148 677 505 294 269 245
48
NaeI GCC'GGC
Cuts at: 2342 2469 2342
Size: 127 7317
NarI GG'CG_CC
Cuts at: 2392 2392
Size: 7444
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5935
Fragments arranged by size:
5935 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7444
NgoMIV G'CCGG_C
Cuts at: 2340 2467 2340
Size: 127 7317
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7444
NotI GC'GGCC_GC
Cuts at: 4891 4891
Size: 7444
NsiI A_TGCA'T
Cuts at: 3724 3724
Size: 7444
NspI r_CATG'y
Cuts at: 1132 1229 2841 3168 3351 4802 6347 1132
Size: 97 1612 327 183 1451 1545 2229
Fragments arranged by size:
2229 1612 1545 1451 327 183 97
PasI CC'CwG_GG
Cuts at: 1348 2387 1348
Size: 1039 6405
PciI A'CATG_T
Cuts at: 2837 3164 6343 2837
Size: 327 3179 3938
Fragments arranged by size:
3938 3179 327
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7444
PfoI T'CCnGG_A
Cuts at: 4614 4723 4614
Size: 109 7335
PmeI GTTT'AAAC
Cuts at: 4748 4748
Size: 7444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5513 5545 7026 7058 5513
Size: 32 1481 32 5899
Fragments arranged by size:
5899 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7444
PsiI TTA'TAA
Cuts at: 5014 5014
Size: 7444
PstI C_TGCA'G
Cuts at: 830 2322 2379 2679 3097 3670 4796 830
Size: 1492 57 300 418 573 1126 3478
Fragments arranged by size:
3478 1492 1126 573 418 300 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7444
PvuII CAG'CTG
Cuts at: 2380 2674 2380
Size: 294 7150
SacI G_AGCT'C
Cuts at: 4501 4501
Size: 7444
SalI G'TCGA_C
Cuts at: 1936 4786 1936
Size: 2850 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 7444
SbfI CC_TGCA'GG
Cuts at: 4796 4796
Size: 7444
ScaI AGT'ACT
Cuts at: 5692 7404 5692
Size: 1712 5732
SfcI C'TryA_G
Cuts at: 826 1045 2318 2375 2675 3093 3666 4792 5927
Size: 219 1273 57 300 418 573 1126 1135
Cuts at: 5927 6608 6799 826
Size: 681 191 1471
Fragments arranged by size:
1471 1273 1135 1126 681 573 418 300
219 191 57
SfoI GGC'GCC
Cuts at: 2393 2393
Size: 7444
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7444
SmaI CCC'GGG
Cuts at: 1872 4771 1872
Size: 2899 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2597 3748 4500 5548 6449 6711
Size: 197 926 654 1151 752 1048 901 262
Cuts at: 6711 820
Size: 1553
Fragments arranged by size:
1553 1151 1048 926 901 752 654 262
197
SnaBI TAC'GTA
Cuts at: 493 4741 5235 493
Size: 4248 494 2702
Fragments arranged by size:
4248 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7444
SphI G_CATG'C
Cuts at: 1132 4802 1132
Size: 3670 3774
SspI AAT'ATT
Cuts at: 5 52 3455 3925 4675 5368 5
Size: 47 3403 470 750 693 2081
Fragments arranged by size:
3403 2081 750 693 470 47
StuI AGG'CCT
Cuts at: 1517 2162 1517
Size: 645 6799
StyI C'CwwG_G
Cuts at: 513 1065 2022 4883 513
Size: 552 957 2861 3074
Fragments arranged by size:
3074 2861 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2065 2279 5471 5488 5641 5826 6271
Size: 1255 485 214 3192 17 153 185 445
Cuts at: 6271 325
Size: 1498
Fragments arranged by size:
3192 1498 1255 485 445 214 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4336 4625 5690
Size: 274 80 33 51 1443 2359 289 1065
Cuts at: 5690 7402 96
Size: 1712 138
Fragments arranged by size:
2359 1712 1443 1065 289 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3924 3956 7404 7436 3924
Size: 32 3448 32 3932
Fragments arranged by size:
3932 3448 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7444
XbaI T'CTAG_A
Cuts at: 4780 4780
Size: 7444
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7444
XmaI C'CCGG_G
Cuts at: 1870 4769 1870
Size: 2899 4545
XmnI GAAnn'nnTTC
Cuts at: 5573 5573
Size: 7444
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5252 276
Size: 53 83 186 936 3718 2468
Fragments arranged by size:
3718 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FspAI NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI XcmI