Restriction map for
pFN21ASDA1382
(Circular) (Six-base) MAPSORT of: pFN21ASDA1382.seq Check: 2456 from: 1 to: 6247
pFN21ASDA1382 6247 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3590 1937
Size: 1653 4594
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 4374 4747 1563
Size: 2811 373 3063
Fragments arranged by size:
3063 2811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4310 5694 5962 1632
Size: 2678 1384 268 1917
Fragments arranged by size:
2678 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 3079 5146 6123 1203
Size: 1876 2067 977 1327
Fragments arranged by size:
2067 1876 1327 977
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6247
AlwNI CAG_nnn'CTG
Cuts at: 2474 3990 5562 2474
Size: 1516 1572 3159
Fragments arranged by size:
3159 1572 1516
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 2188 2216 2662 2741 3556 3782 3934 6043
Size: 695 28 446 79 815 226 152 2109
Cuts at: 6043 1493
Size: 1697
Fragments arranged by size:
2109 1697 815 695 446 226 152 79
28
AseI AT'TA_AT
Cuts at: 160 1032 4801 160
Size: 872 3769 1606
Fragments arranged by size:
3769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 3572 1870
Size: 73 1629 4545
Fragments arranged by size:
4545 1629 73
BamHI G'GATC_C
Cuts at: 3577 3944 3577
Size: 367 5880
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3568 5023 618
Size: 325 217 627 1781 1455 1842
Fragments arranged by size:
1842 1781 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3566 1391
Size: 2175 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2073 2399 928
Size: 971 174 326 4776
Fragments arranged by size:
4776 971 326 174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4438 4472 1903
Size: 34 2501 34 3678
Fragments arranged by size:
3678 2501 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3053 4139 5349 3053
Size: 1086 1210 3951
Fragments arranged by size:
3951 1210 1086
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4858 136
Size: 107 122 71 631 605 3186 1525
Fragments arranged by size:
3186 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6242 6242
Size: 6247
BlpI GC'TnA_GC
Cuts at: 3664 3664
Size: 6247
Bme1580I G_kGCm'C
Cuts at: 1790 4308 5464 1790
Size: 2518 1156 2573
Fragments arranged by size:
2573 2518 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6247
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3978 4936 442
Size: 416 53 83 399 24 2561 958 1753
Fragments arranged by size:
2561 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3549 4907 1239
Size: 2310 1358 2579
Fragments arranged by size:
2579 2310 1358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4372 5237 5535 4372
Size: 865 298 5084
Fragments arranged by size:
5084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4910 882
Size: 617 3411 2219
Fragments arranged by size:
3411 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3544 4038 493
Size: 711 2340 494 2702
Fragments arranged by size:
2702 2340 711 494
BsaBI GATnn'nnATC
Cuts at: 2154 6241 2154
Size: 4087 2160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3256 4054 4436 5071
Size: 53 83 186 936 1723 798 382 635
Cuts at: 5071 275
Size: 1451
Fragments arranged by size:
1723 1451 936 798 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3204 3482 4680 5078 5352 5499 5910 3204
Size: 278 1198 398 274 147 411 3541
Fragments arranged by size:
3541 1198 411 398 278 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6022
Fragments arranged by size:
6022 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6247
BseYI C'CCAG_C
Cuts at: 5450 5946 5450
Size: 496 5751
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5674
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 4458 5486 1540
Size: 275 186 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4308 4393 5464 4308
Size: 85 1071 5091
Fragments arranged by size:
5091 1071 85
BsmI GAATG_Cn'
Cuts at: 3758 3851 3758
Size: 93 6154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3566 4308 4393 5464 1391
Size: 399 1776 742 85 1071 2174
Fragments arranged by size:
2174 1776 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 3204 5910 3204
Size: 2706 3541
BspHI T'CATG_A
Cuts at: 4136 5866 4136
Size: 1730 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2836 3602 844
Size: 1992 766 3489
Fragments arranged by size:
3489 1992 766
BsrBI CCG'CTC
Cuts at: 1394 1823 4134 1394
Size: 429 2311 3507
Fragments arranged by size:
3507 2311 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2272 4742 4916 65
Size: 1183 1024 2470 174 1396
Fragments arranged by size:
2470 1396 1183 1024 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4891 5078 1700
Size: 110 3081 187 2869
Fragments arranged by size:
3081 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3430 96
Size: 3334 2913
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6247
BstBI TT'CG_AA
Cuts at: 2007 5051 2007
Size: 3044 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6247
BstYI r'GATC_y
Cuts at: 1972 2155 3577 3944 4329 4346 5798 5884 5896
Size: 183 1422 367 385 17 1452 86 12
Cuts at: 5896 6242 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1422 385 367 346 183 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2307 513
Size: 552 1242 4453
Fragments arranged by size:
4453 1242 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5508
Fragments arranged by size:
5508 683 56
BtsI GCAGTG_nn'
Cuts at: 2287 3763 4557 4577 2287
Size: 1476 794 20 3957
Fragments arranged by size:
3957 1476 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3551 3551
Size: 6247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2353 5254 809
Size: 507 1037 2901 1802
Fragments arranged by size:
2901 1802 1037 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 3694 4583
Size: 54 1425 50 275 18 1705 159 889
Cuts at: 4583 6212 8
Size: 1629 43
Fragments arranged by size:
1705 1629 1425 889 275 159 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2525 3115 4177 1353
Size: 1172 590 1062 3423
Fragments arranged by size:
3423 1172 1062 590
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4834 5208 5354 387
Size: 1064 138 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3549 4310 4907 5694 5962 1239
Size: 393 1917 761 597 787 268 1524
Fragments arranged by size:
1917 1524 787 761 597 393 268
EcoRI G'AATT_C
Cuts at: 3556 3934 3556
Size: 378 5869
EcoRV GAT'ATC
Cuts at: 3331 3331
Size: 6247
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 5394 1678
Size: 3716 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4888 4920 4962 4994 1373
Size: 32 135 32 3316 32 42 32 2626
Fragments arranged by size:
3316 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3591 3837 669
Size: 988 281 1653 246 3079
Fragments arranged by size:
3079 1653 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2462 748
Size: 1714 4533
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 2429 3846 2429
Size: 1417 4830
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2112 2437 5361 5545 1305
Size: 807 325 2924 184 2007
Fragments arranged by size:
2924 2007 807 325 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3537 10
Size: 54 1425 343 1705 2720
Fragments arranged by size:
2720 1705 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3272 4205 4564 4723 518
Size: 648 2106 933 359 159 2042
Fragments arranged by size:
2106 2042 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3663 4340 5488 5733 1606
Size: 2057 677 1148 245 2120
Fragments arranged by size:
2120 2057 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2307 513
Size: 552 1242 4453
Fragments arranged by size:
4453 1242 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 3083 3605 5150 1132
Size: 97 1854 522 1545 2229
Fragments arranged by size:
2229 1854 1545 522 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6247
PciI A'CATG_T
Cuts at: 3079 5146 3079
Size: 2067 4180
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6247
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4316 4348 5829 5861 4316
Size: 32 1481 32 4702
Fragments arranged by size:
4702 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 2336 3817 2336
Size: 1481 4766
PstI C_TGCA'G
Cuts at: 830 3358 3599 830
Size: 2528 241 3478
Fragments arranged by size:
3478 2528 241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 4495 6207 4495
Size: 1712 4535
SfcI C'TryA_G
Cuts at: 826 1045 2080 3354 3595 4730 5411 5602 826
Size: 219 1035 1274 241 1135 681 191 1471
Fragments arranged by size:
1471 1274 1135 1035 681 241 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3574 1872
Size: 1702 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4351 5252 5514 820
Size: 197 926 2408 901 262 1553
Fragments arranged by size:
2408 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3544 4038 493
Size: 3051 494 2702
Fragments arranged by size:
3051 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 2361 4171 5
Size: 47 2309 1810 2081
Fragments arranged by size:
2309 2081 1810 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6247
StyI C'CwwG_G
Cuts at: 513 1065 2290 2307 3686 513
Size: 552 1225 17 1379 3074
Fragments arranged by size:
3074 1379 1225 552 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4274 4291 4444 4629 5074 325
Size: 1255 2694 17 153 185 445 1498
Fragments arranged by size:
2694 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3430 3469 4493
Size: 274 80 33 51 1443 1453 39 1024
Cuts at: 4493 6205 96
Size: 1712 138
Fragments arranged by size:
1712 1453 1443 1024 274 138 80 51
39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6207 6239 6207
Size: 32 6215
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6247
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 3572 1870
Size: 1702 4545
XmnI GAAnn'nnTTC
Cuts at: 3096 4376 3096
Size: 1280 4967
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI