Restriction map for
pFN21ASDA1379
(Circular) (Six-base) MAPSORT of: pFN21ASDA1379.seq Check: 4768 from: 1 to: 6061
pFN21ASDA1379 6061 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2698 2698
Size: 6061
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2448 4124 5508 5776 1632
Size: 816 1676 1384 268 1917
Fragments arranged by size:
1917 1676 1384 816 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 2079 3804 5376 2079
Size: 1725 1572 2764
Fragments arranged by size:
2764 1725 1572
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 3370 3596 3748 5857 1493
Size: 1877 226 152 2109 1697
Fragments arranged by size:
2109 1877 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 3386 1870
Size: 73 1443 4545
Fragments arranged by size:
4545 1443 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 2223 2276 2784 3152
Size: 325 217 627 266 170 53 508 368
Cuts at: 3152 3291 3382 4837 618
Size: 139 91 1455 1842
Fragments arranged by size:
1842 1455 627 508 368 325 266 217
170 139 91 53
BanII G_rGCy'C
Cuts at: 1391 3234 3380 1391
Size: 1843 146 4072
Fragments arranged by size:
4072 1843 146
BbeI G_GCGC'C
Cuts at: 2057 2227 3156 2057
Size: 170 929 4962
Fragments arranged by size:
4962 929 170
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2639 2743 2874 928
Size: 971 740 104 131 4115
Fragments arranged by size:
4115 971 740 131 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 2495 3020
Size: 107 122 71 631 605 379 444 525
Cuts at: 3020 4672 136
Size: 1652 1525
Fragments arranged by size:
1652 1525 631 605 525 444 379 122
107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3233 3478 3233
Size: 245 5816
Bme1580I G_kGCm'C
Cuts at: 1790 3199 4122 5278 1790
Size: 1409 923 1156 2573
Fragments arranged by size:
2573 1409 1156 923
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2218 2680 2917
Size: 416 53 83 399 24 801 462 237
Cuts at: 2917 3027 3792 4750 442
Size: 110 765 958 1753
Fragments arranged by size:
1753 958 801 765 462 416 399 237
110 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3190 3272 3190
Size: 82 5979
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 2372 2501 2855 4721 1239
Size: 1043 90 129 354 1866 2579
Fragments arranged by size:
2579 1866 1043 354 129 90
Bpu10I CC'TnA_GC
Cuts at: 2577 2577
Size: 6061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 4186 5051 5349 2741
Size: 1445 865 298 3453
Fragments arranged by size:
3453 1445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2057 3134 4724 882
Size: 617 558 1077 1590 2219
Fragments arranged by size:
2219 1590 1077 617 558
BsaAI yAC'GTr
Cuts at: 493 1204 3343 3358 3852 493
Size: 711 2139 15 494 2702
Fragments arranged by size:
2702 2139 711 494 15
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 2224 2379 3153
Size: 53 83 186 936 521 170 155 774
Cuts at: 3153 3868 4250 4885 275
Size: 715 382 635 1451
Fragments arranged by size:
1451 936 774 715 635 521 382 186
170 155 83 53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2696 2726 3174 3204 1178
Size: 30 165 30 1293 30 448 30 4035
Fragments arranged by size:
4035 1293 448 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2945 3287 1186
Size: 889 870 342 3960
Fragments arranged by size:
3960 889 870 342
BseYI C'CCAG_C
Cuts at: 3044 3265 3319 5264 5760 3044
Size: 221 54 1945 496 3345
Fragments arranged by size:
3345 1945 496 221 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2180 2297 2648 1163
Size: 573 444 117 351 4576
Fragments arranged by size:
4576 573 444 351 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3234 4122 4207 5278 3234
Size: 888 85 1071 4017
Fragments arranged by size:
4017 1071 888 85
BsiWI C'GTAC_G
Cuts at: 2187 2187
Size: 6061
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
BsmBI CGTCTCn'nnnn_
Cuts at: 2408 3243 2408
Size: 835 5226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3199 3234 3380 4122 4207 5278 1391
Size: 399 1409 35 146 742 85 1071 2174
Fragments arranged by size:
2174 1409 1071 742 399 146 85 35
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2698 3416 844
Size: 1854 718 3489
Fragments arranged by size:
3489 1854 718
BsrBI CCG'CTC
Cuts at: 1394 1823 2266 3948 1394
Size: 429 443 1682 3507
Fragments arranged by size:
3507 1682 443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2726 4556 4730 65
Size: 1183 1478 1830 174 1396
Fragments arranged by size:
1830 1478 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4705 4892 1700
Size: 110 2895 187 2869
Fragments arranged by size:
2895 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 2167 1925
Size: 242 5819
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2626 794
Size: 1832 4229
BstBI TT'CG_AA
Cuts at: 2007 4865 2007
Size: 2858 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 3343 3343
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 3391 3758 4143 4160 5612 5698 5710 6056
Size: 1419 367 385 17 1452 86 12 346
Cuts at: 6056 1972
Size: 1977
Fragments arranged by size:
1977 1452 1419 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3052 3295 513
Size: 552 1987 243 3279
Fragments arranged by size:
3279 1987 552 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 3064 3142 3577 4371 4391 3064
Size: 78 435 794 20 4734
Fragments arranged by size:
4734 794 435 78 20
ClaI AT'CG_AT
Cuts at: 1554 2118 1554
Size: 564 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DraIII CAC_nnn'GTG
Cuts at: 3099 3099
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 2529 3214
Size: 54 1425 50 275 18 295 404 685
Cuts at: 3214 3508 4397 6026 8
Size: 294 889 1629 43
Fragments arranged by size:
1629 1425 889 685 404 295 294 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3991 1353
Size: 2638 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3232 3232
Size: 6061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2372 2448 2501 2855 4124 4721
Size: 393 650 90 76 53 354 1269 597
Cuts at: 4721 5508 5776 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1269 787 650 597 393 354 268
90 76 53
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 2759 2354
Size: 405 5656
EcoO109I rG'GnC_Cy
Cuts at: 2347 2347
Size: 6061
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 2057 2227 3005 3156 5208 1678
Size: 379 170 778 151 2052 2531
Fragments arranged by size:
2531 2052 778 379 170 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 3020 3052 4702
Size: 32 135 32 458 32 958 32 1650
Cuts at: 4702 4734 4776 4808 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1650 958 458 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KasI G'GCGC_C
Cuts at: 2053 2223 3152 2053
Size: 170 929 4962
Fragments arranged by size:
4962 929 170
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5175 5359 1305
Size: 3870 184 2007
Fragments arranged by size:
3870 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2531 10
Size: 54 1425 343 699 3540
Fragments arranged by size:
3540 1425 699 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2819 2909 4019 4378 4537 518
Size: 648 1653 90 1110 359 159 2042
Fragments arranged by size:
2042 1653 1110 648 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 3054 3312 3319 3477 4154 5302 5547 1606
Size: 1448 258 7 158 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 258 245 158 7
NarI GG'CG_CC
Cuts at: 2054 2224 3153 2054
Size: 170 929 4962
Fragments arranged by size:
4962 929 170
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5509
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2908 3419 4964 1132
Size: 97 1679 511 1545 2229
Fragments arranged by size:
2229 1679 1545 511 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2220 2252 2692 2916 3099 1108
Size: 1112 32 440 224 183 4070
Fragments arranged by size:
4070 1112 440 224 183 32
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2347 2347
Size: 6061
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 6029
PstI C_TGCA'G
Cuts at: 830 2598 3413 830
Size: 1768 815 3478
Fragments arranged by size:
3478 1768 815
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 3319 3319
Size: 6061
RsrII CG'GwC_CG
Cuts at: 2103 2103
Size: 6061
SacI G_AGCT'C
Cuts at: 3234 3234
Size: 6061
SacII CC_GC'GG
Cuts at: 3055 3055
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 2594 3409 4544 5225 5416 826
Size: 219 1549 815 1135 681 191 1471
Fragments arranged by size:
1549 1471 1135 815 681 219 191
SfoI GGC'GCC
Cuts at: 2055 2225 3154 2055
Size: 170 929 4962
Fragments arranged by size:
4962 929 170
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2756 4165 5066 5328 820
Size: 197 926 813 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 813 262 197
SnaBI TAC'GTA
Cuts at: 493 3358 3852 493
Size: 2865 494 2702
Fragments arranged by size:
2865 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 2908 3419 1132
Size: 1776 511 3774
Fragments arranged by size:
3774 1776 511
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 2045 2498 1517
Size: 528 453 5080
Fragments arranged by size:
5080 528 453
StyI C'CwwG_G
Cuts at: 513 1065 2456 3177 3500 513
Size: 552 1391 721 323 3074
Fragments arranged by size:
3074 1391 721 552 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2390 2557 4088 4105 4258 4443 4888
Size: 1255 810 167 1531 17 153 185 445
Cuts at: 4888 325
Size: 1498
Fragments arranged by size:
1531 1498 1255 810 445 185 167 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2688 4307 6019
Size: 274 80 33 51 1443 711 1619 1712
Cuts at: 6019 96
Size: 138
Fragments arranged by size:
1712 1619 1443 711 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3174 3206 6021 6053 3174
Size: 32 2815 32 3182
Fragments arranged by size:
3182 2815 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI SanDI
SapI SexAI SfiI SrfI SwaI XcmI