Restriction map for
pFN21ASDA1369
(Circular) (Six-base) MAPSORT of: pFN21ASDA1369.seq Check: 1181 from: 1 to: 6619
pFN21ASDA1369 6619 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4429 278
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2565 2984 3962 1937
Size: 628 419 978 4594
Fragments arranged by size:
4594 978 628 419
Acc65I G'GTAC_C
Cuts at: 3940 3940
Size: 6619
AclI AA'CG_TT
Cuts at: 1563 4746 5119 1563
Size: 3183 373 3063
Fragments arranged by size:
3183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2669 3371 4682 6066 6334 1632
Size: 1037 702 1311 1384 268 1917
Fragments arranged by size:
1917 1384 1311 1037 702 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 2728 5518 6495 1203
Size: 1525 2790 977 1327
Fragments arranged by size:
2790 1525 1327 977
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6619
AlwNI CAG_nnn'CTG
Cuts at: 3049 3754 4362 5934 3049
Size: 705 608 1572 3734
Fragments arranged by size:
3734 1572 705 608
ApaI G_GGCC'C
Cuts at: 2123 2123
Size: 6619
ApaLI G'TGCA_C
Cuts at: 3338 4676 5832 3338
Size: 1338 1156 4125
Fragments arranged by size:
4125 1338 1156
ApoI r'AATT_y
Cuts at: 1493 2519 3285 3775 3928 4154 4306 6415 1493
Size: 1026 766 490 153 226 152 2109 1697
Fragments arranged by size:
2109 1697 1026 766 490 226 153 152
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 3944 1870
Size: 73 2001 4545
Fragments arranged by size:
4545 2001 73
BamHI G'GATC_C
Cuts at: 2434 3949 4316 2434
Size: 1515 367 4737
Fragments arranged by size:
4737 1515 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3940 5395 618
Size: 325 217 627 2153 1455 1842
Fragments arranged by size:
2153 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2123 2874 2997 3189 3591 3938 1391
Size: 732 751 123 192 402 347 4072
Fragments arranged by size:
4072 751 732 402 347 192 123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 2675 928
Size: 971 429 347 4872
Fragments arranged by size:
4872 971 429 347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4810 4844 1903
Size: 34 2873 34 3678
Fragments arranged by size:
3678 2873 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2468 2546 3905 4511 5721 2468
Size: 78 1359 606 1210 3366
Fragments arranged by size:
3366 1359 1210 606 78
BclI T'GATC_A
Cuts at: 1548 3382 1548
Size: 1834 4785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3058 5230 136
Size: 107 122 71 631 605 1386 2172 1525
Fragments arranged by size:
2172 1525 1386 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3346 6614 3346
Size: 3268 3351
BlpI GC'TnA_GC
Cuts at: 2868 4036 2868
Size: 1168 5451
Bme1580I G_kGCm'C
Cuts at: 1790 2123 3342 4680 5836 1790
Size: 333 1219 1338 1156 2573
Fragments arranged by size:
2573 1338 1219 1156 333
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6619
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4350 5308 442
Size: 416 53 83 399 24 2933 958 1753
Fragments arranged by size:
2933 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 6619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2981 3011 5279 1239
Size: 1022 720 30 2268 2579
Fragments arranged by size:
2579 2268 1022 720 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2269 2999 3852 4744 5609 5907 2269
Size: 730 853 892 865 298 2981
Fragments arranged by size:
2981 892 865 853 730 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3727 5282 882
Size: 617 2228 1555 2219
Fragments arranged by size:
2228 2219 1555 617
BsaAI yAC'GTr
Cuts at: 493 1204 2257 3916 4410 493
Size: 711 1053 1659 494 2702
Fragments arranged by size:
2702 1659 1053 711 494
BsaBI GATnn'nnATC
Cuts at: 6613 6613
Size: 6619
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4426 4808 5443 275
Size: 53 83 186 936 2893 382 635 1451
Fragments arranged by size:
2893 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5052 5450 5724 5871 6282 5052
Size: 398 274 147 411 5389
Fragments arranged by size:
5389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6394
Fragments arranged by size:
6394 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 3092 1186
Size: 889 1017 4713
Fragments arranged by size:
4713 1017 889
BseYI C'CCAG_C
Cuts at: 2075 5822 6318 2075
Size: 3747 496 2376
Fragments arranged by size:
3747 2376 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3146 3403 1163
Size: 573 1410 257 4379
Fragments arranged by size:
4379 1410 573 257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4069 4830 5858 1540
Size: 275 186 2068 761 1028 2301
Fragments arranged by size:
2301 2068 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 2793 2997 3189 3342 3591 4680 4765 5836
Size: 546 204 192 153 249 1089 85 1071
Cuts at: 5836 2247
Size: 3030
Fragments arranged by size:
3030 1089 1071 546 249 204 192 153
85
BsmI GAATG_Cn'
Cuts at: 4130 4223 4130
Size: 93 6526
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2247 2793 2874 2997 3189 3342
Size: 399 333 124 546 81 123 192 153
Cuts at: 3342 3591 3938 4680 4765 5836 1391
Size: 249 347 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 399 347 333 249
192 153 124 123 85 81
BspEI T'CCGG_A
Cuts at: 6282 6282
Size: 6619
BspHI T'CATG_A
Cuts at: 4508 6238 4508
Size: 1730 4889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2669 3974 844
Size: 1825 1305 3489
Fragments arranged by size:
3489 1825 1305
BsrBI CCG'CTC
Cuts at: 1394 1823 4506 1394
Size: 429 2683 3507
Fragments arranged by size:
3507 2683 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2825 5114 5288 65
Size: 1183 1577 2289 174 1396
Fragments arranged by size:
2289 1577 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2095 5263 5450 1700
Size: 110 285 3168 187 2869
Fragments arranged by size:
3168 2869 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 3561 96
Size: 3465 3154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 2748 4679 5691 2748
Size: 1931 1012 3676
Fragments arranged by size:
3676 1931 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6619
BstBI TT'CG_AA
Cuts at: 2007 5423 2007
Size: 3416 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6619
BstXI CCAn_nnnn'nTGG
Cuts at: 3005 3779 3005
Size: 774 5845
BstYI r'GATC_y
Cuts at: 1972 2434 3346 3533 3949 4316 4701 4718 6170
Size: 462 912 187 416 367 385 17 1452
Cuts at: 6170 6256 6268 6614 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 912 462 416 385 367 346
187 86 17 12
BstZ17I GTA'TAC
Cuts at: 2566 2566
Size: 6619
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3711 487
Size: 683 56 2485 3395
Fragments arranged by size:
3395 2485 683 56
BtsI GCAGTG_nn'
Cuts at: 4135 4929 4949 4135
Size: 794 20 5805
Fragments arranged by size:
5805 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6584
DraI TTT'AAA
Cuts at: 3923 3923
Size: 6619
DraIII CAC_nnn'GTG
Cuts at: 2718 2718
Size: 6619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5626 809
Size: 507 4310 1802
Fragments arranged by size:
4310 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 3267 4066
Size: 54 1425 50 275 18 267 1170 799
Cuts at: 4066 4955 6584 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1170 889 799 275 267 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4066 1537
Size: 275 2254 4090
Fragments arranged by size:
4090 2254 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2554 2799 3461 4549 1353
Size: 699 502 245 662 1088 3423
Fragments arranged by size:
3423 1088 699 662 502 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5206 5580 5726 387
Size: 1064 138 3617 374 146 1280
Fragments arranged by size:
3617 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2995 3187 3589 2995
Size: 192 402 6025
Fragments arranged by size:
6025 402 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2669 2981 3011 3371 4682 5279
Size: 393 629 408 312 30 360 1311 597
Cuts at: 5279 6066 6334 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1311 787 629 597 408 393 360
312 268 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2621 2621
Size: 6619
EcoO109I rG'GnC_Cy
Cuts at: 2119 2653 2119
Size: 534 6085
EcoRI G'AATT_C
Cuts at: 3928 4306 3928
Size: 378 6241
FspI TGC'GCA
Cuts at: 5125 5125
Size: 6619
HaeII r_GCGC'y
Cuts at: 1678 5766 1678
Size: 4088 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2362 2394 2413 2445 2762
Size: 32 135 32 790 32 19 32 317
Cuts at: 2762 2794 3536 3568 5260 5292 5334 5366 1373
Size: 32 742 32 1692 32 42 32 2626
Fragments arranged by size:
2626 1692 790 742 317 135 42 32
32 32 32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3121 3790 3963 4209 669
Size: 988 281 1047 136 669 173 246 3079
Fragments arranged by size:
3079 1047 988 669 281 246 173 136
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6619
HpaI GTT'AAC
Cuts at: 3790 4209 3790
Size: 419 6200
KpnI G_GTAC'C
Cuts at: 3944 3944
Size: 6619
MfeI C'AATT_G
Cuts at: 3033 4218 3033
Size: 1185 5434
MluI A'CGCG_T
Cuts at: 6495 6495
Size: 6619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2820 3507 5733 5917 1305
Size: 1515 687 2226 184 2007
Fragments arranged by size:
2226 2007 1515 687 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3269 10
Size: 54 1425 343 1437 3360
Fragments arranged by size:
3360 1437 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2259 2733 3170 4577 4936 5095 518
Size: 648 1093 474 437 1407 359 159 2042
Fragments arranged by size:
2042 1407 1093 648 474 437 359 159
MspA1I CmG'CkG
Cuts at: 1606 3049 3178 3751 4035 4712 5860 6105 1606
Size: 1443 129 573 284 677 1148 245 2120
Fragments arranged by size:
2120 1443 1148 677 573 284 245 129
NaeI GCC'GGC
Cuts at: 2097 2097
Size: 6619
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6067
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6619
NgoMIV G'CCGG_C
Cuts at: 2095 2095
Size: 6619
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 4066 4066
Size: 6619
NspI r_CATG'y
Cuts at: 1132 1229 2732 3977 5522 1132
Size: 97 1503 1245 1545 2229
Fragments arranged by size:
2229 1545 1503 1245 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6619
PciI A'CATG_T
Cuts at: 2728 5518 2728
Size: 2790 3829
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6619
PfoI T'CCnGG_A
Cuts at: 2997 2997
Size: 6619
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PmlI CAC'GTG
Cuts at: 2257 2257
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3612 3644 4688 4720 6201 6233 3612
Size: 32 1044 32 1481 32 3998
Fragments arranged by size:
3998 1481 1044 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2653 2653
Size: 6619
PshAI GACnn'nnGTC
Cuts at: 1613 3408 1613
Size: 1795 4824
PsiI TTA'TAA
Cuts at: 4189 4189
Size: 6619
PspOMI G'GGCC_C
Cuts at: 2119 2119
Size: 6619
PstI C_TGCA'G
Cuts at: 830 3759 3971 830
Size: 2929 212 3478
Fragments arranged by size:
3478 2929 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
PvuII CAG'CTG
Cuts at: 3049 3178 3751 3049
Size: 129 573 5917
Fragments arranged by size:
5917 573 129
SacI G_AGCT'C
Cuts at: 2997 3189 3591 2997
Size: 192 402 6025
Fragments arranged by size:
6025 402 192
SalI G'TCGA_C
Cuts at: 1936 2983 3961 1936
Size: 1047 978 4594
Fragments arranged by size:
4594 1047 978
SbfI CC_TGCA'GG
Cuts at: 3971 3971
Size: 6619
ScaI AGT'ACT
Cuts at: 4867 6579 4867
Size: 1712 4907
SfcI C'TryA_G
Cuts at: 826 1045 3431 3755 3800 3967 5102 5783 5974
Size: 219 2386 324 45 167 1135 681 191
Cuts at: 5974 826
Size: 1471
Fragments arranged by size:
2386 1471 1135 681 324 219 191 167
45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 3946 1872
Size: 2074 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2248 2978 3867 4723 5624 5886
Size: 197 926 305 730 889 856 901 262
Cuts at: 5886 820
Size: 1553
Fragments arranged by size:
1553 926 901 889 856 730 305 262
197
SnaBI TAC'GTA
Cuts at: 493 3916 4410 493
Size: 3423 494 2702
Fragments arranged by size:
3423 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3977 1132
Size: 2845 3774
SspI AAT'ATT
Cuts at: 5 52 2698 3364 4543 5
Size: 47 2646 666 1179 2081
Fragments arranged by size:
2646 2081 1179 666 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6619
StyI C'CwwG_G
Cuts at: 513 1065 2487 4058 513
Size: 552 1422 1571 3074
Fragments arranged by size:
3074 1571 1422 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3560 4646 4663 4816 5001 5446 325
Size: 1255 1980 1086 17 153 185 445 1498
Fragments arranged by size:
1980 1498 1255 1086 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2940 3561 4865
Size: 274 80 33 51 1443 963 621 1304
Cuts at: 4865 6577 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1304 963 621 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6579 6611 6579
Size: 32 6587
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6619
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3034 3034
Size: 6619
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 3944 1870
Size: 2074 4545
XmnI GAAnn'nnTTC
Cuts at: 2730 4748 2730
Size: 2018 4601
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4427 276
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI Bsu36I EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI