Restriction map for
pFN21ASDA1368
(Circular) (Six-base) MAPSORT of: pFN21ASDA1368.seq Check: 7137 from: 1 to: 7870
pFN21ASDA1368 7870 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2846 5680 278
Size: 53 83 186 936 1310 2834 2468
Fragments arranged by size:
2834 2468 1310 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 4083 5213 1937
Size: 397 1749 1130 4594
Fragments arranged by size:
4594 1749 1130 397
Acc65I G'GTAC_C
Cuts at: 5191 5191
Size: 7870
AclI AA'CG_TT
Cuts at: 1563 2541 2963 5997 6370 1563
Size: 978 422 3034 373 3063
Fragments arranged by size:
3063 3034 978 422 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2713 2873 5093 5933 7317 7585 1632
Size: 1081 160 2220 840 1384 268 1917
Fragments arranged by size:
2220 1917 1384 1081 840 268 160
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7673
AflIII A'CryG_T
Cuts at: 1203 6769 7746 1203
Size: 5566 977 1327
Fragments arranged by size:
5566 1327 977
AgeI A'CCGG_T
Cuts at: 4766 6701 4766
Size: 1935 5935
AhdI GACnn_n'nnGTC
Cuts at: 6599 6599
Size: 7870
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7870
AlwNI CAG_nnn'CTG
Cuts at: 2585 2879 3092 3308 5613 7185 2585
Size: 294 213 216 2305 1572 3270
Fragments arranged by size:
3270 2305 1572 294 216 213
ApaLI G'TGCA_C
Cuts at: 4985 5927 7083 4985
Size: 942 1156 5772
Fragments arranged by size:
5772 1156 942
ApoI r'AATT_y
Cuts at: 1493 2286 2495 5179 5405 5557 7666 1493
Size: 793 209 2684 226 152 2109 1697
Fragments arranged by size:
2684 2109 1697 793 226 209 152
AseI AT'TA_AT
Cuts at: 160 1032 6424 160
Size: 872 5392 1606
Fragments arranged by size:
5392 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7870
AvaI C'yCGr_G
Cuts at: 1870 1943 4069 4744 5023 5195 1870
Size: 73 2126 675 279 172 4545
Fragments arranged by size:
4545 2126 675 279 172 73
BamHI G'GATC_C
Cuts at: 5200 5567 5200
Size: 367 7503
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3641 5191 6646 618
Size: 325 217 627 1854 1550 1455 1842
Fragments arranged by size:
1854 1842 1550 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4146 4166 4460 5189 1391
Size: 2755 20 294 729 4072
Fragments arranged by size:
4072 2755 729 294 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3477 3933 4057 928
Size: 971 1578 456 124 4741
Fragments arranged by size:
4741 1578 971 456 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6061 6095 1903
Size: 34 4124 34 3678
Fragments arranged by size:
4124 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2145 2464 5762 6972 2145
Size: 319 3298 1210 3043
Fragments arranged by size:
3298 3043 1210 319
BclI T'GATC_A
Cuts at: 1548 2208 4096 1548
Size: 660 1888 5322
Fragments arranged by size:
5322 1888 660
BfrBI ATG'CAT
Cuts at: 2414 2414
Size: 7870
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4595 6481 136
Size: 107 122 71 631 605 2923 1886 1525
Fragments arranged by size:
2923 1886 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7865 7865
Size: 7870
BlpI GC'TnA_GC
Cuts at: 4187 5287 4187
Size: 1100 6770
Bme1580I G_kGCm'C
Cuts at: 1790 3231 4989 5931 7087 1790
Size: 1441 1758 942 1156 2573
Fragments arranged by size:
2573 1758 1441 1156 942
BmgBI CAC'GTC
Cuts at: 1318 4114 1318
Size: 2796 5074
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3051 5601 6559
Size: 416 53 83 399 24 1634 2550 958
Cuts at: 6559 442
Size: 1753
Fragments arranged by size:
2550 1753 1634 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2849 2894 2901 3440 3589 4622 4643 4900
Size: 1610 45 7 539 149 1033 21 257
Cuts at: 4900 6530 1239
Size: 1630 2579
Fragments arranged by size:
2579 1630 1610 1033 539 257 149 45
21 7
Bpu10I CC'TnA_GC
Cuts at: 2787 3108 2787
Size: 321 7549
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3837 5995 6860 7158 3837
Size: 2158 865 298 4549
Fragments arranged by size:
4549 2158 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3603 6533 882
Size: 617 2104 2930 2219
Fragments arranged by size:
2930 2219 2104 617
BsaAI yAC'GTr
Cuts at: 493 1204 4561 5167 5661 493
Size: 711 3357 606 494 2702
Fragments arranged by size:
3357 2702 711 606 494
BsaBI GATnn'nnATC
Cuts at: 2207 2933 7864 2207
Size: 726 4931 2213
Fragments arranged by size:
4931 2213 726
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2843 5677 6059 6694
Size: 53 83 186 936 1310 2834 382 635
Cuts at: 6694 275
Size: 1451
Fragments arranged by size:
2834 1451 1310 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2137 2616 4766 6303 6701 6975 7122 7533 2137
Size: 479 2150 1537 398 274 147 411 2474
Fragments arranged by size:
2474 2150 1537 479 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2822 2852 4877 4907 1178
Size: 30 165 30 1419 30 2025 30 4141
Fragments arranged by size:
4141 2025 1419 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3437 3753 4789 4840 4885 1186
Size: 2251 316 1036 51 45 4171
Fragments arranged by size:
4171 2251 1036 316 51 45
BseYI C'CCAG_C
Cuts at: 2069 3308 3824 4382 4499 4511 4995 5033 7073
Size: 1239 516 558 117 12 484 38 2040
Cuts at: 7073 7569 2069
Size: 496 2370
Fragments arranged by size:
2370 2040 1239 558 516 496 484 117
38 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4145 4417 1163
Size: 573 2409 272 4616
Fragments arranged by size:
4616 2409 573 272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5320 6081 7109 1540
Size: 275 186 3319 761 1028 2301
Fragments arranged by size:
3319 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4146 4989 5931 6016 7087 4146
Size: 843 942 85 1071 4929
Fragments arranged by size:
4929 1071 942 843 85
BsiWI C'GTAC_G
Cuts at: 5078 5078
Size: 7870
BsmI GAATG_Cn'
Cuts at: 3805 3885 5381 5474 3805
Size: 80 1496 93 6201
Fragments arranged by size:
6201 1496 93 80
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3231 4146 4166 4460 4989 5189 5931
Size: 399 1441 915 20 294 529 200 742
Cuts at: 5931 6016 7087 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1441 1071 915 742 529 399 294
200 85 20
BspEI T'CCGG_A
Cuts at: 7533 7533
Size: 7870
BspHI T'CATG_A
Cuts at: 2211 5759 7489 2211
Size: 3548 1730 2592
Fragments arranged by size:
3548 2592 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4488 5225 844
Size: 3644 737 3489
Fragments arranged by size:
3644 3489 737
BsrBI CCG'CTC
Cuts at: 1394 1823 3211 5757 1394
Size: 429 1388 2546 3507
Fragments arranged by size:
3507 2546 1388 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2229 3024 4029 6365 6539 65
Size: 1183 981 795 1005 2336 174 1396
Fragments arranged by size:
2336 1396 1183 1005 981 795 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3561 4766 6514 6701 1700
Size: 110 1751 1205 1748 187 2869
Fragments arranged by size:
2869 1751 1748 1205 187 110
BsrGI T'GTAC_A
Cuts at: 96 5159 96
Size: 5063 2807
BssHII G'CGCG_C
Cuts at: 1925 2858 1925
Size: 933 6937
BssSI C'ACGA_G
Cuts at: 4849 5930 6942 4849
Size: 1081 1012 5777
Fragments arranged by size:
5777 1081 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2552 3005 794
Size: 1758 453 5659
Fragments arranged by size:
5659 1758 453
BstBI TT'CG_AA
Cuts at: 2007 6674 2007
Size: 4667 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7870
BstXI CCAn_nnnn'nTGG
Cuts at: 4519 4931 5003 4519
Size: 412 72 7386
Fragments arranged by size:
7386 412 72
BstYI r'GATC_y
Cuts at: 1972 2813 5200 5567 5952 5969 7421 7507 7519
Size: 841 2387 367 385 17 1452 86 12
Cuts at: 7519 7865 1972
Size: 346 1977
Fragments arranged by size:
2387 1977 1452 841 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2697 2757 3248 4387 4452 4473 4669
Size: 552 1632 60 491 1139 65 21 196
Cuts at: 4669 513
Size: 3714
Fragments arranged by size:
3714 1632 1139 552 491 196 65 60
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7131
Fragments arranged by size:
7131 683 56
BtsI GCAGTG_nn'
Cuts at: 2054 2748 3231 3300 3942 5386 6180 6200 2054
Size: 694 483 69 642 1444 794 20 3724
Fragments arranged by size:
3724 1444 794 694 642 483 69 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7870
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7835
DraI TTT'AAA
Cuts at: 5174 5174
Size: 7870
DraIII CAC_nnn'GTG
Cuts at: 3331 3331
Size: 7870
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6877 809
Size: 507 5561 1802
Fragments arranged by size:
5561 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2508 3375 3559
Size: 54 1425 50 275 18 678 867 184
Cuts at: 3559 4026 4287 5005 5317 6206 7835 8
Size: 467 261 718 312 889 1629 43
Fragments arranged by size:
1629 1425 889 867 718 678 467 312
275 261 184 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5317 1537
Size: 275 3505 4090
Fragments arranged by size:
4090 3505 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4807 5800 1353
Size: 3454 993 3423
Fragments arranged by size:
3454 3423 993
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6457 6831 6977 387
Size: 1064 138 4868 374 146 1280
Fragments arranged by size:
4868 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4144 4144
Size: 7870
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2713 2849 2873 2894 2901 3440 3589
Size: 393 1081 136 24 21 7 539 149
Cuts at: 3589 4622 4643 4900 5093 5933 6530 7317 7585
Size: 1033 21 257 193 840 597 787 268
Cuts at: 7585 1239
Size: 1524
Fragments arranged by size:
1524 1081 1033 840 787 597 539 393
268 257 193 149 136 24 21 21
7
EcoO109I rG'GnC_Cy
Cuts at: 4739 4739
Size: 7870
EcoRI G'AATT_C
Cuts at: 2495 5179 5557 2495
Size: 2684 378 4808
Fragments arranged by size:
4808 2684 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 7870
FspI TGC'GCA
Cuts at: 6376 6376
Size: 7870
HaeII r_GCGC'y
Cuts at: 1678 4601 7017 1678
Size: 2923 2416 2531
Fragments arranged by size:
2923 2531 2416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2700 2732 4196 4228 6511
Size: 32 135 32 1128 32 1464 32 2283
Cuts at: 6511 6543 6585 6617 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2283 1464 1128 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4353 4816 5214 5460 669
Size: 988 281 2415 463 398 246 3079
Fragments arranged by size:
3079 2415 988 463 398 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7870
HpaI GTT'AAC
Cuts at: 5460 5460
Size: 7870
KpnI G_GTAC'C
Cuts at: 5195 5195
Size: 7870
MfeI C'AATT_G
Cuts at: 5469 5469
Size: 7870
MluI A'CGCG_T
Cuts at: 7746 7746
Size: 7870
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6984 7168 1305
Size: 5679 184 2007
Fragments arranged by size:
5679 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2510 3377 4028 5007 10
Size: 54 1425 343 678 867 651 979 2873
Fragments arranged by size:
2873 1425 979 867 678 651 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 2364 3488 4982 5138 5828 6187
Size: 648 1032 166 1124 1494 156 690 359
Cuts at: 6187 6346 518
Size: 159 2042
Fragments arranged by size:
2042 1494 1124 1032 690 648 359 166
159 156
MspA1I CmG'CkG
Cuts at: 1606 2502 3305 3496 4006 4191 4389 4723 5286
Size: 896 803 191 510 185 198 334 563
Cuts at: 5286 5963 7111 7356 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 896 803 677 563 510 334
245 198 191 185
NcoI C'CATG_G
Cuts at: 513 1065 2757 3248 4452 4669 513
Size: 552 1692 491 1204 217 3714
Fragments arranged by size:
3714 1692 1204 552 491 217
NdeI CA'TA_TG
Cuts at: 387 2521 387
Size: 2134 5736
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7870
NotI GC'GGCC_GC
Cuts at: 5317 5317
Size: 7870
NsiI A_TGCA'T
Cuts at: 2416 2416
Size: 7870
NspI r_CATG'y
Cuts at: 1132 1229 2343 2537 3019 3493 4447 5228 6773
Size: 97 1114 194 482 474 954 781 1545
Cuts at: 6773 1132
Size: 2229
Fragments arranged by size:
2229 1545 1114 954 781 482 474 194
97
PasI CC'CwG_GG
Cuts at: 1348 2990 4735 1348
Size: 1642 1745 4483
Fragments arranged by size:
4483 1745 1642
PciI A'CATG_T
Cuts at: 6769 6769
Size: 7870
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3620 1108
Size: 2512 5358
PfoI T'CCnGG_A
Cuts at: 2826 2878 3417 2826
Size: 52 539 7279
Fragments arranged by size:
7279 539 52
PmeI GTTT'AAAC
Cuts at: 5174 5174
Size: 7870
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5939 5971 7452 7484 5939
Size: 32 1481 32 6325
Fragments arranged by size:
6325 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4739 4739
Size: 7870
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7870
PsiI TTA'TAA
Cuts at: 2979 5440 2979
Size: 2461 5409
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3512 3544 3512
Size: 32 7838
PstI C_TGCA'G
Cuts at: 830 2456 3001 3650 4368 5222 830
Size: 1626 545 649 718 854 3478
Fragments arranged by size:
3478 1626 854 718 649 545
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7870
PvuII CAG'CTG
Cuts at: 3305 3496 4006 3305
Size: 191 510 7169
Fragments arranged by size:
7169 510 191
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 7870
SacI G_AGCT'C
Cuts at: 4146 4146
Size: 7870
SacII CC_GC'GG
Cuts at: 4390 4390
Size: 7870
SalI G'TCGA_C
Cuts at: 1936 5212 1936
Size: 3276 4594
SanDI GG'GwC_CC
Cuts at: 4739 4739
Size: 7870
SbfI CC_TGCA'GG
Cuts at: 5222 5222
Size: 7870
ScaI AGT'ACT
Cuts at: 6118 7830 6118
Size: 1712 6158
SfcI C'TryA_G
Cuts at: 826 1045 2452 2997 3646 4364 5218 6353 7034
Size: 219 1407 545 649 718 854 1135 681
Cuts at: 7034 7225 826
Size: 191 1471
Fragments arranged by size:
1471 1407 1135 854 718 681 649 545
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7870
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7870
SmaI CCC'GGG
Cuts at: 1872 4746 5197 1872
Size: 2874 451 4545
Fragments arranged by size:
4545 2874 451
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3852 5023 5974 6875 7137 820
Size: 197 926 1909 1171 951 901 262 1553
Fragments arranged by size:
1909 1553 1171 951 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5167 5661 493
Size: 4674 494 2702
Fragments arranged by size:
4674 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7870
SphI G_CATG'C
Cuts at: 1132 3493 5228 1132
Size: 2361 1735 3774
Fragments arranged by size:
3774 2361 1735
SspI AAT'ATT
Cuts at: 5 52 5794 5
Size: 47 5742 2081
Fragments arranged by size:
5742 2081 47
StuI AGG'CCT
Cuts at: 1517 4730 1517
Size: 3213 4657
StyI C'CwwG_G
Cuts at: 513 1065 2757 3248 3556 4452 4669 5309 513
Size: 552 1692 491 308 896 217 640 3074
Fragments arranged by size:
3074 1692 896 640 552 491 308 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3676 4163 5897 5914 6067 6252 6697
Size: 1255 2096 487 1734 17 153 185 445
Cuts at: 6697 325
Size: 1498
Fragments arranged by size:
2096 1734 1498 1255 487 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5159 6116 7828
Size: 274 80 33 51 1443 3182 957 1712
Cuts at: 7828 96
Size: 138
Fragments arranged by size:
3182 1712 1443 957 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2822 2854 4787 4819 7830 7862 2822
Size: 32 1933 32 3011 32 2830
Fragments arranged by size:
3011 2830 1933 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2606 5062 1316
Size: 1290 2456 4124
Fragments arranged by size:
4124 2456 1290
XbaI T'CTAG_A
Cuts at: 2318 5206 2318
Size: 2888 4982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3292 3621 3292
Size: 329 7541
XhoI C'TCGA_G
Cuts at: 1943 5023 1943
Size: 3080 4790
XmaI C'CCGG_G
Cuts at: 1870 4744 5195 1870
Size: 2874 451 4545
Fragments arranged by size:
4545 2874 451
XmnI GAAnn'nnTTC
Cuts at: 3102 5999 3102
Size: 2897 4973
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2844 5678 276
Size: 53 83 186 936 1310 2834 2468
Fragments arranged by size:
2834 2468 1310 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI SapI SexAI SfiI SfoI SrfI SwaI