Restriction map for
pFN21ASDA1355
(Circular) (Six-base) MAPSORT of: pFN21ASDA1355.seq Check: 1923 from: 1 to: 8266
pFN21ASDA1355 8266 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2645 2645
Size: 8266
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6076 278
Size: 53 83 186 936 4540 2468
Fragments arranged by size:
4540 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 3525 4182 5609 1937
Size: 694 894 657 1427 4594
Fragments arranged by size:
4594 1427 894 694 657
Acc65I G'GTAC_C
Cuts at: 3227 5587 3227
Size: 2360 5906
AclI AA'CG_TT
Cuts at: 1563 6393 6766 1563
Size: 4830 373 3063
Fragments arranged by size:
4830 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2397 3790 4454 5382 6329 7713 7981 1632
Size: 765 1393 664 928 947 1384 268 1917
Fragments arranged by size:
1917 1393 1384 947 928 765 664 268
AfeI AGC'GCT
Cuts at: 5113 5113
Size: 8266
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8069
AflIII A'CryG_T
Cuts at: 1203 7165 8142 1203
Size: 5962 977 1327
Fragments arranged by size:
5962 1327 977
AgeI A'CCGG_T
Cuts at: 7097 7097
Size: 8266
AhdI GACnn_n'nnGTC
Cuts at: 2702 3694 5528 6995 2702
Size: 992 1834 1467 3973
Fragments arranged by size:
3973 1834 1467 992
AleI CACnn'nnGTG
Cuts at: 1166 2924 3560 5264 1166
Size: 1758 636 1704 4168
Fragments arranged by size:
4168 1758 1704 636
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2531 4217 1184
Size: 1347 1686 5233
Fragments arranged by size:
5233 1686 1347
AlwNI CAG_nnn'CTG
Cuts at: 2473 2963 3645 3710 4474 4660 4781 4801 5193
Size: 490 682 65 764 186 121 20 392
Cuts at: 5193 6009 7581 2473
Size: 816 1572 3158
Fragments arranged by size:
3158 1572 816 764 682 490 392 186
121 65 20
ApaI G_GGCC'C
Cuts at: 3240 4568 4593 3240
Size: 1328 25 6913
Fragments arranged by size:
6913 1328 25
ApaLI G'TGCA_C
Cuts at: 6323 7479 6323
Size: 1156 7110
ApoI r'AATT_y
Cuts at: 1493 5294 5465 5575 5801 5953 8062 1493
Size: 3801 171 110 226 152 2109 1697
Fragments arranged by size:
3801 2109 1697 226 171 152 110
AseI AT'TA_AT
Cuts at: 160 1032 6820 160
Size: 872 5788 1606
Fragments arranged by size:
5788 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8266
AvaI C'yCGr_G
Cuts at: 1870 1943 2850 3501 3860 3930 5073 5591 1870
Size: 73 907 651 359 70 1143 518 4545
Fragments arranged by size:
4545 1143 907 651 518 359 73 70
BamHI G'GATC_C
Cuts at: 2598 2859 3952 4445 4486 4516 5596 5963 2598
Size: 261 1093 493 41 30 1080 367 4901
Fragments arranged by size:
4901 1093 1080 493 367 261 41 30
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 2713 3227 3587 4069
Size: 325 217 627 244 682 514 360 482
Cuts at: 4069 4702 4741 5587 7042 618
Size: 633 39 846 1455 1842
Fragments arranged by size:
1842 1455 846 682 633 627 514 482
360 325 244 217 39
BanII G_rGCy'C
Cuts at: 1391 3240 4406 4554 4568 4593 4863 5004 5506
Size: 1849 1166 148 14 25 270 141 502
Cuts at: 5506 5585 1391
Size: 79 4072
Fragments arranged by size:
4072 1849 1166 502 270 148 141 79
25 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2331 2385 2818 4731 5393 928
Size: 971 432 54 433 1913 662 3801
Fragments arranged by size:
3801 1913 971 662 433 432 54
BbvCI CC'TCA_GC
Cuts at: 2051 2772 2956 4218 5165 2051
Size: 721 184 1262 947 5152
Fragments arranged by size:
5152 1262 947 721 184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6457 6491 1903
Size: 34 4520 34 3678
Fragments arranged by size:
4520 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4885 4957 6158 7368 4885
Size: 72 1201 1210 5783
Fragments arranged by size:
5783 1210 1201 72
BclI T'GATC_A
Cuts at: 1548 3162 1548
Size: 1614 6652
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3506 4008 6877
Size: 107 122 71 631 605 1834 502 2869
Cuts at: 6877 136
Size: 1525
Fragments arranged by size:
2869 1834 1525 631 605 502 122 107
71
BglII A'GATC_T
Cuts at: 8261 8261
Size: 8266
BlpI GC'TnA_GC
Cuts at: 5367 5683 5367
Size: 316 7950
Bme1580I G_kGCm'C
Cuts at: 1790 2718 2931 3149 3240 3471 3539 4568 4593
Size: 928 213 218 91 231 68 1029 25
Cuts at: 4593 4705 6327 7483 1790
Size: 112 1622 1156 2573
Fragments arranged by size:
2573 1622 1156 1029 928 231 218 213
112 91 68 25
BmgBI CAC'GTC
Cuts at: 1318 2529 1318
Size: 1211 7055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2696 3481 3700
Size: 416 53 83 399 24 1279 785 219
Cuts at: 3700 3751 4445 4689 4758 5997 6955 442
Size: 51 694 244 69 1239 958 1753
Fragments arranged by size:
1753 1279 1239 958 785 694 416 399
244 219 83 69 53 51 24
BmtI G_CTAG'C
Cuts at: 1056 3779 1056
Size: 2723 5543
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5099 5305 5099
Size: 206 8060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2495 2632 4196 4424 4568 4632 4734
Size: 1046 210 137 1564 228 144 64 102
Cuts at: 4734 4778 4914 5160 5339 5376 6926 1239
Size: 44 136 246 179 37 1550 2579
Fragments arranged by size:
2579 1564 1550 1046 246 228 210 179
144 137 136 102 64 44 37
Bpu10I CC'TnA_GC
Cuts at: 2051 2772 2956 4218 4405 4802 5165 2051
Size: 721 184 1262 187 397 363 5152
Fragments arranged by size:
5152 1262 721 397 363 187 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3248 4874 6391 7256 7554 3248
Size: 1626 1517 865 298 3960
Fragments arranged by size:
3960 1626 1517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2318 6929 882
Size: 617 819 4611 2219
Fragments arranged by size:
4611 2219 819 617
BsaAI yAC'GTr
Cuts at: 493 1204 2266 3529 5563 6057 493
Size: 711 1062 1263 2034 494 2702
Fragments arranged by size:
2702 2034 1263 1062 711 494
BsaBI GATnn'nnATC
Cuts at: 8260 8260
Size: 8266
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6073 6455 7090 275
Size: 53 83 186 936 4540 382 635 1451
Fragments arranged by size:
4540 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3829 4169 6699 7097 7371 7518 7929 3829
Size: 340 2530 398 274 147 411 4166
Fragments arranged by size:
4166 2530 411 398 340 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4254 4284 4776 4806 1178
Size: 30 165 30 2851 30 492 30 4638
Fragments arranged by size:
4638 2851 492 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3351 4871 5266 5411 1186
Size: 2165 1520 395 145 4041
Fragments arranged by size:
4041 2165 1520 395 145
BseYI C'CCAG_C
Cuts at: 2120 2137 2679 2895 2930 2986 3274 3532 3790
Size: 17 542 216 35 56 288 258 258
Cuts at: 3790 4020 4142 4457 4502 4653 4707 4862 4985
Size: 230 122 315 45 151 54 155 123
Cuts at: 4985 5193 7469 7965 2120
Size: 208 2276 496 2421
Fragments arranged by size:
2421 2276 542 496 315 288 258 258
230 216 208 155 151 123 122 56
54 45 35 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2486 2600 3190 3840 4371 4857 1163
Size: 573 750 114 590 650 531 486 4572
Fragments arranged by size:
4572 750 650 590 573 531 486 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2083 2163 2167 4374 5716 6477
Size: 275 186 82 80 4 2207 1342 761
Cuts at: 6477 7505 1540
Size: 1028 2301
Fragments arranged by size:
2301 2207 1342 1028 761 275 186 82
80 4
BsiHKAI G_wGCw'C
Cuts at: 2982 6327 6412 7483 2982
Size: 3345 85 1071 3765
Fragments arranged by size:
3765 3345 1071 85
BsmI GAATG_Cn'
Cuts at: 3482 3684 5777 5870 3482
Size: 202 2093 93 5878
Fragments arranged by size:
5878 2093 202 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4165 4165
Size: 8266
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2718 2931 2982 3149 3240 3471 3539
Size: 399 928 213 51 167 91 231 68
Cuts at: 3539 4406 4554 4568 4593 4705 4863 5004 5506
Size: 867 148 14 25 112 158 141 502
Cuts at: 5506 5585 6327 6412 7483 1391
Size: 79 742 85 1071 2174
Fragments arranged by size:
2174 1071 928 867 742 502 399 231
213 167 158 148 141 112 91 85
79 68 51 25 14
BspEI T'CCGG_A
Cuts at: 3829 7929 3829
Size: 4100 4166
BspHI T'CATG_A
Cuts at: 6155 7885 6155
Size: 1730 6536
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2157 2645 5621 844
Size: 1313 488 2976 3489
Fragments arranged by size:
3489 2976 1313 488
BsrBI CCG'CTC
Cuts at: 1394 1823 3289 5041 6153 1394
Size: 429 1466 1752 1112 3507
Fragments arranged by size:
3507 1752 1466 1112 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2941 3496 6761 6935 65
Size: 1183 1693 555 3265 174 1396
Fragments arranged by size:
3265 1693 1396 1183 555 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2162 2166 2909 4002 4232 4289 4305
Size: 110 352 4 743 1093 230 57 16
Cuts at: 4305 6910 7097 1700
Size: 2605 187 2869
Fragments arranged by size:
2869 2605 1093 743 352 230 187 110
57 16 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8266
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8266
BssSI C'ACGA_G
Cuts at: 4109 6326 7338 4109
Size: 2217 1012 5037
Fragments arranged by size:
5037 2217 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2783 4394 794
Size: 1989 1611 4666
Fragments arranged by size:
4666 1989 1611
BstBI TT'CG_AA
Cuts at: 2007 7070 2007
Size: 5063 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8266
BstXI CCAn_nnnn'nTGG
Cuts at: 5099 5429 5099
Size: 330 7936
BstYI r'GATC_y
Cuts at: 1972 2598 2859 3952 4348 4445 4486 4516 5596
Size: 626 261 1093 396 97 41 30 1080
Cuts at: 5596 5963 6348 6365 7817 7903 7915 8261 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1093 1080 626 396 385 367
346 261 97 86 41 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 2094 5436 2094
Size: 3342 4924
BtgI C'CryG_G
Cuts at: 513 1065 2022 2503 3463 3567 3902 513
Size: 552 957 481 960 104 335 4877
Fragments arranged by size:
4877 960 957 552 481 335 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3151 487
Size: 683 56 1925 5602
Fragments arranged by size:
5602 1925 683 56
BtsI GCAGTG_nn'
Cuts at: 3074 3487 3711 3924 4992 5104 5415 5782 6576
Size: 413 224 213 1068 112 311 367 794
Cuts at: 6576 6596 3074
Size: 20 4744
Fragments arranged by size:
4744 1068 794 413 367 311 224 213
112 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8231
DraI TTT'AAA
Cuts at: 5570 5570
Size: 8266
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4378 7273 809
Size: 507 3062 2895 1802
Fragments arranged by size:
3062 2895 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2160 2164
Size: 54 1425 50 275 18 213 117 4
Cuts at: 2164 2554 2889 3507 3660 4152 4230 4266 4287
Size: 390 335 618 153 492 78 36 21
Cuts at: 4287 5340 5713 6602 8231 8
Size: 1053 373 889 1629 43
Fragments arranged by size:
1629 1425 1053 889 618 492 390 373
335 275 213 153 117 78 54 50
43 36 21 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2160 2164 5713 1537
Size: 275 348 4 3549 4090
Fragments arranged by size:
4090 3549 348 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 6196 1353
Size: 4843 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2591 3334 4094 4260 4331 5468
Size: 1064 138 1002 743 760 166 71 1137
Cuts at: 5468 6853 7227 7373 387
Size: 1385 374 146 1280
Fragments arranged by size:
1385 1280 1137 1064 1002 760 743 374
166 146 138 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2397 2495 2632 3790 4196 4424
Size: 393 653 112 98 137 1158 406 228
Cuts at: 4424 4454 4568 4632 4734 4778 4914 5160 5339
Size: 30 114 64 102 44 136 246 179
Cuts at: 5339 5376 5382 6329 6926 7713 7981 1239
Size: 37 6 947 597 787 268 1524
Fragments arranged by size:
1524 1158 947 787 653 597 406 393
268 246 228 179 137 136 114 112
102 98 64 44 37 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2555 4082 5175 5459 2555
Size: 1527 1093 284 5362
Fragments arranged by size:
5362 1527 1093 284
EcoO109I rG'GnC_Cy
Cuts at: 2292 2548 2696 3109 3237 3355 4535 4564 4589
Size: 256 148 413 128 118 1180 29 25
Cuts at: 4589 4778 2292
Size: 189 5780
Fragments arranged by size:
5780 1180 413 256 189 148 128 118
29 25
EcoRI G'AATT_C
Cuts at: 5294 5465 5575 5953 5294
Size: 171 110 378 7607
Fragments arranged by size:
7607 378 171 110
FseI GG_CCGG'CC
Cuts at: 2166 2170 2166
Size: 4 8262
FspI TGC'GCA
Cuts at: 6772 6772
Size: 8266
HaeII r_GCGC'y
Cuts at: 1678 2667 4339 4579 4903 5115 7413 1678
Size: 989 1672 240 324 212 2298 2531
Fragments arranged by size:
2531 2298 1672 989 324 240 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2296 2328 2688
Size: 32 135 32 515 32 177 32 360
Cuts at: 2688 2720 3627 3659 3680 3712 3871 3903 4254
Size: 32 907 32 21 32 159 32 351
Cuts at: 4254 4286 4437 4469 4508 4540 4776 4808 5514
Size: 32 151 32 39 32 236 32 706
Cuts at: 5514 5546 6907 6939 6981 7013 1373
Size: 32 1361 32 42 32 2626
Fragments arranged by size:
2626 1361 907 706 515 360 351 236
177 159 151 135 42 39 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 5610 5856 669
Size: 988 281 3672 246 3079
Fragments arranged by size:
3672 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8266
HpaI GTT'AAC
Cuts at: 5856 5856
Size: 8266
KpnI G_GTAC'C
Cuts at: 3231 5591 3231
Size: 2360 5906
MfeI C'AATT_G
Cuts at: 5865 5865
Size: 8266
MluI A'CGCG_T
Cuts at: 8142 8142
Size: 8266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2439 4394 7380 7564 1305
Size: 1134 1955 2986 184 2007
Fragments arranged by size:
2986 2007 1955 1134 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2556 2891 3509 3662 5342
Size: 54 1425 343 724 335 618 153 1680
Cuts at: 5342 10
Size: 2934
Fragments arranged by size:
2934 1680 1425 724 618 343 335 153
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2711 2924 3032 3560 5264 6224 6583
Size: 648 1545 213 108 528 1704 960 359
Cuts at: 6583 6742 518
Size: 159 2042
Fragments arranged by size:
2042 1704 1545 960 648 528 359 213
159 108
MspA1I CmG'CkG
Cuts at: 1606 2683 2802 3051 3090 3118 3854 3904 4201
Size: 1077 119 249 39 28 736 50 297
Cuts at: 4201 4385 5193 5445 5682 6359 7507 7752 1606
Size: 184 808 252 237 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 808 736 677 297 252
249 245 237 184 119 50 39 28
NaeI GCC'GGC
Cuts at: 2164 2168 2911 4004 4234 4291 2164
Size: 4 743 1093 230 57 6139
Fragments arranged by size:
6139 1093 743 230 57 4
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6757
Fragments arranged by size:
6757 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8266
NgoMIV G'CCGG_C
Cuts at: 2162 2166 2909 4002 4232 4289 2162
Size: 4 743 1093 230 57 6139
Fragments arranged by size:
6139 1093 743 230 57 4
NheI G'CTAG_C
Cuts at: 1052 3775 1052
Size: 2723 5543
NotI GC'GGCC_GC
Cuts at: 5713 5713
Size: 8266
NspI r_CATG'y
Cuts at: 1132 1229 2183 3037 5234 5624 7169 1132
Size: 97 954 854 2197 390 1545 2229
Fragments arranged by size:
2229 2197 1545 954 854 390 97
PasI CC'CwG_GG
Cuts at: 1348 3142 3172 3190 4015 4953 5300 1348
Size: 1794 30 18 825 938 347 4314
Fragments arranged by size:
4314 1794 938 825 347 30 18
PciI A'CATG_T
Cuts at: 7165 7165
Size: 8266
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 4660 4781 5193 1108
Size: 920 2632 121 412 4181
Fragments arranged by size:
4181 2632 920 412 121
PfoI T'CCnGG_A
Cuts at: 2358 2436 2808 4780 5176 2358
Size: 78 372 1972 396 5448
Fragments arranged by size:
5448 1972 396 372 78
PmeI GTTT'AAAC
Cuts at: 5570 5570
Size: 8266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6335 6367 7848 7880 6335
Size: 32 1481 32 6721
Fragments arranged by size:
6721 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2548 2696 3355 4535 4778 2548
Size: 148 659 1180 243 6036
Fragments arranged by size:
6036 1180 659 243 148
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8266
PsiI TTA'TAA
Cuts at: 5836 5836
Size: 8266
PspOMI G'GGCC_C
Cuts at: 3236 4564 4589 3236
Size: 1328 25 6913
Fragments arranged by size:
6913 1328 25
PspXI vC'TCGA_Gb
Cuts at: 5073 5073
Size: 8266
PstI C_TGCA'G
Cuts at: 830 2286 2847 3117 3633 3747 4999 5152 5618
Size: 1456 561 270 516 114 1252 153 466
Cuts at: 5618 830
Size: 3478
Fragments arranged by size:
3478 1456 1252 561 516 466 270 153
114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8266
PvuII CAG'CTG
Cuts at: 2683 2802 3090 3118 3854 4201 4385 5193 2683
Size: 119 288 28 736 347 184 808 5756
Fragments arranged by size:
5756 808 736 347 288 184 119 28
SacII CC_GC'GG
Cuts at: 3905 3905
Size: 8266
SalI G'TCGA_C
Cuts at: 1936 5608 1936
Size: 3672 4594
SanDI GG'GwC_CC
Cuts at: 3355 4535 3355
Size: 1180 7086
SbfI CC_TGCA'GG
Cuts at: 5618 5618
Size: 8266
ScaI AGT'ACT
Cuts at: 6514 8226 6514
Size: 1712 6554
SfcI C'TryA_G
Cuts at: 826 1045 2282 2797 2843 3113 3629 3743 4678
Size: 219 1237 515 46 270 516 114 935
Cuts at: 4678 4995 5148 5614 6749 7430 7621 826
Size: 317 153 466 1135 681 191 1471
Fragments arranged by size:
1471 1237 1135 935 681 516 515 466
317 270 219 191 153 114 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 3506 3506
Size: 8266
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8266
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8266
SmaI CCC'GGG
Cuts at: 1872 2852 3862 3932 5593 1872
Size: 980 1010 70 1661 4545
Fragments arranged by size:
4545 1661 1010 980 70
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3263 4853 5073 6370 7271 7533
Size: 197 926 1320 1590 220 1297 901 262
Cuts at: 7533 820
Size: 1553
Fragments arranged by size:
1590 1553 1320 1297 926 901 262 220
197
SnaBI TAC'GTA
Cuts at: 493 5563 6057 493
Size: 5070 494 2702
Fragments arranged by size:
5070 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8266
SphI G_CATG'C
Cuts at: 1132 3037 5624 1132
Size: 1905 2587 3774
Fragments arranged by size:
3774 2587 1905
SspI AAT'ATT
Cuts at: 5 52 3305 6190 5
Size: 47 3253 2885 2081
Fragments arranged by size:
3253 2885 2081 47
StuI AGG'CCT
Cuts at: 1517 4077 5055 1517
Size: 2560 978 4728
Fragments arranged by size:
4728 2560 978
StyI C'CwwG_G
Cuts at: 513 1065 2022 2551 3096 3294 3349 3381 3453
Size: 552 957 529 545 198 55 32 72
Cuts at: 3453 3582 3699 5705 513
Size: 129 117 2006 3074
Fragments arranged by size:
3074 2006 957 552 545 529 198 129
117 72 55 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2392 2684 2693 2909 3682 3690 3770
Size: 1255 812 292 9 216 773 8 80
Cuts at: 3770 4777 6293 6310 6463 6648 7093 325
Size: 1007 1516 17 153 185 445 1498
Fragments arranged by size:
1516 1498 1255 1007 812 773 445 292
216 185 153 80 17 9 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6512 8224 96
Size: 274 80 33 51 1443 4535 1712 138
Fragments arranged by size:
4535 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8226 8258 8226
Size: 32 8234
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8266
XbaI T'CTAG_A
Cuts at: 5602 5602
Size: 8266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3493 3565 4171 3493
Size: 72 606 7588
Fragments arranged by size:
7588 606 72
XhoI C'TCGA_G
Cuts at: 1943 5073 1943
Size: 3130 5136
XmaI C'CCGG_G
Cuts at: 1870 2850 3860 3930 5591 1870
Size: 980 1010 70 1661 4545
Fragments arranged by size:
4545 1661 1010 980 70
XmnI GAAnn'nnTTC
Cuts at: 3837 4962 6395 3837
Size: 1125 1433 5708
Fragments arranged by size:
5708 1433 1125
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6074 276
Size: 53 83 186 936 4540 2468
Fragments arranged by size:
4540 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI
NsiI PacI PmlI PsrI RsrII SacI SapI SexAI
SfoI SrfI SwaI